FastQCFastQC Report
Thu 26 May 2016
SRR937074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937074_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574973
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20720.36036474756205944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15710.27323022124517155No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10420.1812259010423098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10200.1773996344176161No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG601.4923844E-647.4934739
GGTATCA22650.027.4877571
GTATCAA32650.027.0748421
ATCAACG37350.023.5323983
GAGTACT20350.023.4570512-13
TCAACGC37600.023.3678024
TATCAAC37950.023.1603452
CAACGCA38050.023.0914425
GTATAGG1652.4146193E-623.0431731
GCATGCG1050.002196648622.6159429
AACGCAG39000.022.5289576
AGAGTAC32050.021.63509410-11
GTGTATC2005.3476106E-721.3869461
GTACTTT22850.021.10125214-15
AGTACTT21400.020.86343412-13
ACGCAGA43000.020.433247
CGCAGAG42950.020.3464498
GTACATG21100.020.2719861
CTAGACT2451.7547427E-719.3850964
GCAGAGT44850.019.2727159
Produced by FastQC (version 0.11.5)