##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937074_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 574973 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.238626857261124 33.0 31.0 34.0 31.0 34.0 2 32.308960594671404 33.0 31.0 34.0 31.0 34.0 3 32.453141277938265 34.0 31.0 34.0 31.0 34.0 4 36.111335662718076 37.0 35.0 37.0 35.0 37.0 5 36.02343240465204 37.0 35.0 37.0 35.0 37.0 6 36.05242854881882 37.0 35.0 37.0 35.0 37.0 7 36.012593634831546 37.0 35.0 37.0 35.0 37.0 8 35.99158047421357 37.0 35.0 37.0 35.0 37.0 9 37.68475041436729 39.0 37.0 39.0 35.0 39.0 10-11 37.60093256552916 39.0 37.0 39.0 35.0 39.0 12-13 37.54253591038188 39.0 37.0 39.0 35.0 39.0 14-15 38.807976200621596 40.0 38.0 41.0 35.0 41.0 16-17 38.74707508004724 40.0 38.0 41.0 35.0 41.0 18-19 38.77076227927225 40.0 38.0 41.0 34.5 41.0 20-21 38.84530404036363 40.0 38.0 41.0 35.0 41.0 22-23 38.77817393164548 40.0 38.0 41.0 35.0 41.0 24-25 38.713732644837236 40.0 38.0 41.0 34.0 41.0 26-27 38.54900751861392 40.0 38.0 41.0 34.0 41.0 28-29 38.400508371697455 40.0 38.0 41.0 34.0 41.0 30-31 38.36517540823657 40.0 38.0 41.0 34.0 41.0 32-33 38.281924542543734 40.0 38.0 41.0 34.0 41.0 34-35 38.16172150692293 40.0 38.0 41.0 34.0 41.0 36-37 38.01959831157289 40.0 38.0 41.0 33.5 41.0 38-39 37.88051438937133 40.0 37.5 41.0 33.0 41.0 40-41 37.68434604755353 40.0 37.0 41.0 33.0 41.0 42-43 37.485401053614694 40.0 37.0 41.0 33.0 41.0 44-45 37.248156869974764 40.0 37.0 41.0 32.0 41.0 46-47 36.944446087033654 40.0 37.0 41.0 31.0 41.0 48-49 36.614538421804156 40.0 36.5 41.0 30.5 41.0 50-51 36.38323016906881 39.5 36.0 41.0 30.0 41.0 52-53 36.19714056138288 39.0 36.0 41.0 30.0 41.0 54-55 35.94450782906328 39.0 35.5 40.0 29.0 41.0 56-57 35.718878973447445 39.0 35.0 40.0 28.5 41.0 58-59 35.41937099655114 38.5 35.0 40.0 28.0 41.0 60-61 35.12645028549167 38.0 34.5 40.0 27.0 41.0 62-63 34.822975165790396 38.0 34.0 40.0 26.5 41.0 64-65 34.459904204197414 38.0 34.0 40.0 26.0 41.0 66-67 34.07762451454242 37.0 34.0 40.0 26.0 41.0 68-69 33.65455943148635 36.5 33.0 39.0 24.5 40.5 70-71 33.29199023258484 36.0 33.0 39.0 24.0 40.0 72-73 32.91940491118713 36.0 32.5 39.0 22.5 40.0 74-75 32.441568560610676 35.0 32.0 38.0 22.0 40.0 76-77 30.9116341115148 34.0 30.5 36.0 20.0 39.0 78-79 31.600175138658685 35.0 31.0 37.0 20.5 39.0 80-81 31.21161776292104 35.0 31.0 36.0 17.5 39.0 82-83 31.05968975934522 34.5 31.0 36.0 18.0 37.5 84-85 30.712413452457767 34.0 31.0 35.5 18.0 37.0 86-87 30.343991804832577 34.0 31.0 35.0 14.5 37.0 88-89 29.967981105199723 34.0 30.5 35.0 8.5 36.0 90-91 29.553755567652743 34.0 30.0 35.0 4.5 36.0 92-93 29.18468345470135 34.0 29.5 35.0 2.0 35.5 94-95 28.745824586545805 34.0 29.0 35.0 2.0 35.0 96-97 28.1913272449315 34.0 29.0 35.0 2.0 35.0 98-99 27.500220879936972 33.5 28.5 35.0 2.0 35.0 100-101 24.472508274301575 31.0 21.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2095494604575876 1101 2 0.22304743836403418 1101 3 0.19024797428321705 1101 4 0.14494832756079745 1101 5 0.15439869930509076 1101 6 0.280450534850452 1101 7 0.13336521108481492 1101 8 0.1703989342095511 1101 9 0.23499364452198535 1101 10-11 0.29730796040863794 1101 12-13 0.2626979845859623 1101 14-15 0.37001302276021875 1101 16-17 0.23188074176810858 1101 18-19 0.28091942822454286 1101 20-21 0.2767508517176367 1101 22-23 0.42511510411795683 1101 24-25 0.27294676107789684 1101 26-27 0.2701656909108081 1101 28-29 0.18748498595282825 1101 30-31 0.20737280928317858 1101 32-33 0.3045165543759296 1101 34-35 0.20461547458286589 1101 36-37 0.33950722740764405 1101 38-39 0.33029379759069144 1101 40-41 0.5479767360916767 1101 42-43 0.5476494163176753 1101 44-45 0.29269017997254565 1101 46-47 0.4352778719027768 1101 48-49 0.5098215282666771 1101 50-51 0.42302800012880937 1101 52-53 0.4459138545865997 1101 54-55 0.46143323817286586 1101 56-57 0.690077397470148 1101 58-59 0.6158433805244883 1101 60-61 0.6437879407884495 1101 62-63 0.5842223766781345 1101 64-65 0.7335368108012474 1101 66-67 0.7848574326037294 1101 68-69 0.8529895473463966 1101 70-71 0.8169273769206953 1101 72-73 0.8663545061622528 1101 74-75 0.7763484361666073 1101 76-77 0.8167174963100869 1101 78-79 0.9640564386533619 1101 80-81 1.2976908819060071 1101 82-83 1.097527564647649 1101 84-85 0.9728487250559894 1101 86-87 1.0767858600554447 1101 88-89 0.9940175504878894 1101 90-91 0.8449665940431395 1101 92-93 1.0165249161804937 1101 94-95 1.1810462709351377 1101 96-97 1.1001568159634587 1101 98-99 1.151599689940685 1101 100-101 1.1472993544820511 1102 1 0.3127561502760017 1102 2 0.3010981994345414 1102 3 0.1589153666016756 1102 4 0.1155248751860185 1102 5 0.15529712533180628 1102 6 0.13735891267315026 1102 7 0.14064764512824723 1102 8 0.12270460638771397 1102 9 0.28407016623106784 1102 10-11 0.28532174779265773 1102 12-13 0.29966735171394276 1102 14-15 0.24174543002364146 1102 16-17 0.24063175681674664 1102 18-19 0.16778685855333464 1102 20-21 0.1447081419265288 1102 22-23 0.26008547333650966 1102 24-25 0.37618368638759137 1102 26-27 0.3214148486364081 1102 28-29 0.2691078543507004 1102 30-31 0.34750999202648103 1102 32-33 0.32852785381096083 1102 34-35 0.2709910166620375 1102 36-37 0.21765387817907822 1102 38-39 0.2782498783799312 1102 40-41 0.4194197465099663 1102 42-43 0.22812938314501707 1102 44-45 0.16015506360909626 1102 46-47 0.20285765040620873 1102 48-49 0.40746143220420095 1102 50-51 0.5324675205075309 1102 52-53 0.40663795140928727 1102 54-55 0.5066093815063795 1102 56-57 0.5794414155077732 1102 58-59 0.42424107226069907 1102 60-61 0.4431236238513918 1102 62-63 0.46349637933885646 1102 64-65 0.7023467418643321 1102 66-67 0.25677298055721565 1102 68-69 0.20303014929183405 1102 70-71 0.376940507762626 1102 72-73 0.7783260329681738 1102 74-75 0.7981870995851139 1102 76-77 0.7693557588923703 1102 78-79 0.6143527464679401 1102 80-81 0.8279177344777509 1102 82-83 0.5344520104742827 1102 84-85 0.6314425155499528 1102 86-87 0.6331750512992471 1102 88-89 0.4276679245441102 1102 90-91 0.26429830329865567 1102 92-93 0.6161594986715535 1102 94-95 0.7790567756086837 1102 96-97 0.7567205091855271 1102 98-99 0.5744300774732984 1102 100-101 1.3107912945639413 1103 1 0.10130567121901635 1103 2 0.11009024330758876 1103 3 0.011659787946570077 1103 4 0.07674407148273588 1103 5 0.08110736199297008 1103 6 0.15008664718237696 1103 7 0.196404890743068 1103 8 0.13457386526006587 1103 9 0.08959038293008348 1103 10-11 0.04289604271064462 1103 12-13 0.22457831503393066 1103 14-15 0.278369126939225 1103 16-17 0.31902885523804514 1103 18-19 0.2707028026409475 1103 20-21 0.2776425771426432 1103 22-23 0.23746688130550098 1103 24-25 0.0891074797236584 1103 26-27 0.04837075378807043 1103 28-29 0.0780803612949299 1103 30-31 0.12611751466346277 1103 32-33 0.08018763083713765 1103 34-35 0.25205685955984336 1103 36-37 0.1819547376890256 1103 38-39 0.2675752452432292 1103 40-41 0.5112456104157133 1103 42-43 0.07343195845700023 1103 44-45 0.1676193196485869 1103 46-47 0.1790007618574876 1103 48-49 0.15045830514635838 1103 50-51 0.2447259090326881 1103 52-53 0.13024040019092098 1103 54-55 0.046302674708556424 1103 56-57 -0.017160652878374094 1103 58-59 -0.0376400811536044 1103 60-61 0.1551378007663189 1103 62-63 0.1438819923444825 1103 64-65 -0.14085676283085746 1103 66-67 -0.14102532248813304 1103 68-69 -0.13521844548583317 1103 70-71 -0.004669151294983465 1103 72-73 0.17147109926157356 1103 74-75 0.07316879807974175 1103 76-77 0.19780750325280394 1103 78-79 0.2972591979066017 1103 80-81 0.5100128287549239 1103 82-83 0.25260020842244657 1103 84-85 0.22768315075296996 1103 86-87 0.21464289523826352 1103 88-89 0.08520337545067846 1103 90-91 0.1682128142950745 1103 92-93 0.1633406071509249 1103 94-95 0.2810845938968569 1103 96-97 0.5306420419840094 1103 98-99 0.7883542764879472 1103 100-101 0.6250991069220859 1104 1 0.13307099131616695 1104 2 0.1192607373187684 1104 3 0.07576093577160492 1104 4 0.19300505224134668 1104 5 0.02329239108583181 1104 6 0.21909991110646843 1104 7 0.17591504108771971 1104 8 0.119229177261154 1104 9 0.34271871688134325 1104 10-11 0.13804975441512823 1104 12-13 0.1594768872515644 1104 14-15 0.25077744091514376 1104 16-17 0.24168875248614086 1104 18-19 0.1753493376015598 1104 20-21 0.17290221864594457 1104 22-23 0.15687549037158277 1104 24-25 0.3677981874103793 1104 26-27 0.42027438793680716 1104 28-29 0.23761896135697214 1104 30-31 0.38877045081049033 1104 32-33 0.4857239761077565 1104 34-35 0.5000710541528832 1104 36-37 0.3751010797060843 1104 38-39 0.33827354960980216 1104 40-41 0.4169506867230055 1104 42-43 0.3594999199893536 1104 44-45 0.5022871162331128 1104 46-47 0.41983594265695245 1104 48-49 0.5494580251890326 1104 50-51 0.49219153859618103 1104 52-53 0.40307016343782465 1104 54-55 0.30578225673983894 1104 56-57 0.38682533412253406 1104 58-59 0.5903912135355256 1104 60-61 0.453681266032703 1104 62-63 0.5167518575866481 1104 64-65 0.5885886946723744 1104 66-67 0.23616874846970148 1104 68-69 0.1845344354263716 1104 70-71 0.4803659946989782 1104 72-73 0.48757696852906207 1104 74-75 0.6688635083995749 1104 76-77 0.701779870708144 1104 78-79 0.8431714426347092 1104 80-81 0.8246667031373178 1104 82-83 0.7281175619934146 1104 84-85 0.3179232330755575 1104 86-87 0.3258328651995974 1104 88-89 0.6003112346851118 1104 90-91 0.6500791460218025 1104 92-93 0.6001451591057076 1104 94-95 0.8108501107207786 1104 96-97 0.9456901265462534 1104 98-99 1.2859277274537995 1104 100-101 1.385326250676389 1105 1 0.12799449397210338 1105 2 0.04793743399063288 1105 3 0.0357554224495118 1105 4 0.08277465899304559 1105 5 0.047794866779497625 1105 6 0.09834755469140077 1105 7 0.10291653604621587 1105 8 0.09517200835205131 1105 9 0.00970558033120028 1105 10-11 -0.004164268507572899 1105 12-13 0.05666403272905285 1105 14-15 0.2093575484249257 1105 16-17 -0.07409207175551558 1105 18-19 -0.062160337218465145 1105 20-21 -0.05604696336790482 1105 22-23 -0.08458893279709656 1105 24-25 0.09626813450369553 1105 26-27 0.16220744348353122 1105 28-29 0.12486728350462073 1105 30-31 0.2164632315393149 1105 32-33 0.11341463632807347 1105 34-35 -0.03534355827117963 1105 36-37 0.01296381919100753 1105 38-39 0.3189207288033771 1105 40-41 0.13943655883513628 1105 42-43 0.12557178402234825 1105 44-45 0.34825019818016045 1105 46-47 0.21536060947205726 1105 48-49 0.16145798277886314 1105 50-51 0.20762247893566865 1105 52-53 0.1526752187300886 1105 54-55 0.16876135912858814 1105 56-57 0.025262731410400363 1105 58-59 -0.09210504906824468 1105 60-61 -0.12408944338341854 1105 62-63 -0.01637472616814506 1105 64-65 -0.049152115573917854 1105 66-67 -0.44446040640163886 1105 68-69 -0.3240483227190296 1105 70-71 -0.3822229341261476 1105 72-73 -0.3713084922044132 1105 74-75 -0.18058250875534299 1105 76-77 -0.5863336189066644 1105 78-79 -0.19614384517131 1105 80-81 -0.10624606974562312 1105 82-83 -0.2894847790479673 1105 84-85 -0.7247106505297332 1105 86-87 -0.579235150110474 1105 88-89 -0.5542080990511202 1105 90-91 -0.44620360258130987 1105 92-93 -0.13118217318857717 1105 94-95 -0.21558308796026537 1105 96-97 -0.007489045819276896 1105 98-99 0.1237438157517694 1105 100-101 0.3735669708010718 1106 1 0.042157949937191574 1106 2 0.0192172821500165 1106 3 -0.11732969239699997 1106 4 0.0024758648529967786 1106 5 0.024390841184811052 1106 6 -0.048523573998060954 1106 7 -0.09258961827203649 1106 8 -0.2449975621526832 1106 9 0.015433699257854983 1106 10-11 -0.07514492474087575 1106 12-13 -0.35380711641797546 1106 14-15 -0.08340729183557016 1106 16-17 -0.17248728923081558 1106 18-19 -0.12245617637652373 1106 20-21 -0.3700351093440162 1106 22-23 -0.29117626956275444 1106 24-25 -0.26936684566383207 1106 26-27 -0.07850283189806362 1106 28-29 -0.18808235678314844 1106 30-31 -0.29247985096151297 1106 32-33 -0.18206367296762238 1106 34-35 -0.08246841762539248 1106 36-37 -0.1534272385788853 1106 38-39 0.10814101134511844 1106 40-41 0.10124767865965367 1106 42-43 -0.005502977021897948 1106 44-45 -0.12587666146955456 1106 46-47 0.045264458904362925 1106 48-49 0.23355101925016442 1106 50-51 -0.16204292618099458 1106 52-53 -0.08254134735354057 1106 54-55 -0.09291650237368287 1106 56-57 0.10883899835892663 1106 58-59 -0.023316478029343557 1106 60-61 0.04825419829092681 1106 62-63 -0.09612656450789814 1106 64-65 0.09436340376417007 1106 66-67 -0.12681831154671386 1106 68-69 -0.24759100516075705 1106 70-71 0.00841147777560991 1106 72-73 -0.20472422407389956 1106 74-75 -0.41835342945953613 1106 76-77 -0.5637493166801981 1106 78-79 -0.38462055195754274 1106 80-81 0.27692275064829985 1106 82-83 0.18339615790863206 1106 84-85 -0.10070937761441812 1106 86-87 -0.04321136250548818 1106 88-89 -0.46464941424541095 1106 90-91 -0.5384402398118091 1106 92-93 -0.3137617019826031 1106 94-95 0.2650688833536776 1106 96-97 0.259563189169981 1106 98-99 -0.03706971351959609 1106 100-101 -0.3184145151002511 1107 1 0.07057179029347793 1107 2 0.02605524035488571 1107 3 0.11638733398189771 1107 4 0.08874170932028136 1107 5 0.0630239347099888 1107 6 0.06311397310710731 1107 7 0.13384409190268087 1107 8 0.11579037892302324 1107 9 0.10340903327635687 1107 10-11 0.14300341373120062 1107 12-13 0.12257691921066538 1107 14-15 0.18308808329290116 1107 16-17 0.041592875209062186 1107 18-19 0.10413169618257712 1107 20-21 -0.053483208007946814 1107 22-23 0.0028901915540657797 1107 24-25 0.1399873852371627 1107 26-27 0.1505396484034094 1107 28-29 0.15343871207604565 1107 30-31 0.10779250258422479 1107 32-33 0.14249610777921617 1107 34-35 0.19558399758528822 1107 36-37 -0.21145053422808502 1107 38-39 -0.17943178755862021 1107 40-41 -0.0029917869107478623 1107 42-43 -0.17445981155427148 1107 44-45 -0.12004589750929284 1107 46-47 -0.10576329032483045 1107 48-49 0.020803502977464916 1107 50-51 -0.05323511528684577 1107 52-53 -0.07216793180027281 1107 54-55 -0.021114515391829514 1107 56-57 0.0833354012366243 1107 58-59 0.16711860247767873 1107 60-61 0.3119746505005807 1107 62-63 0.20754763898644768 1107 64-65 -0.0640607140844125 1107 66-67 -0.035573022064014026 1107 68-69 0.14941139292078276 1107 70-71 -0.09208526768516379 1107 72-73 0.017944498091225114 1107 74-75 0.005226563688800923 1107 76-77 -0.10248347221291709 1107 78-79 0.11138492937167754 1107 80-81 0.2739028808298656 1107 82-83 0.26962603115288886 1107 84-85 0.24977366452329974 1107 86-87 0.1990000112526502 1107 88-89 0.05850235005743443 1107 90-91 -0.005810914700468572 1107 92-93 0.23047433775703396 1107 94-95 0.5425663139806041 1107 96-97 0.6191640798859801 1107 98-99 0.8841635855554308 1107 100-101 1.0722038473526467 1108 1 0.06578466974261232 1108 2 0.10294012057988766 1108 3 0.009639200920119606 1108 4 0.14279660215318302 1108 5 0.11133350701141609 1108 6 0.18402562086193086 1108 7 0.15680597726782253 1108 8 0.2091702644821396 1108 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2316 90-91 -1.496834482327813 2316 92-93 -1.44624831066373 2316 94-95 -1.3105085356980162 2316 96-97 -1.2577659083647639 2316 98-99 -1.6031596572395124 2316 100-101 -1.8853149860152456 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 3.0 5 5.0 6 20.0 7 66.0 8 161.0 9 289.0 10 529.0 11 871.0 12 1309.0 13 1639.0 14 2217.0 15 3013.0 16 4006.0 17 5555.0 18 5081.0 19 3378.0 20 2673.0 21 2658.0 22 2817.0 23 3167.0 24 3426.0 25 4096.0 26 4808.0 27 6080.0 28 7513.0 29 9152.0 30 11454.0 31 14621.0 32 18871.0 33 24914.0 34 34430.0 35 48071.0 36 76614.0 37 130746.0 38 128124.0 39 12593.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.85143619621549 22.076939269367468 15.04217864120233 22.02944589321471 2 14.715658564160542 23.73039961734139 39.55420272209418 21.99973909640388 3 18.23214990789911 27.254732455171478 30.935463278791747 23.577654358137668 4 12.366145888589552 20.088943306903108 39.01052745085421 28.534383353653126 5 12.697976491541754 40.00751380139214 34.64819060009531 12.646319106970791 6 28.754393684573014 40.27893483694017 18.15737434627365 12.809297132213166 7 25.965741000012176 35.26304713438718 22.130604393597615 16.640607472003033 8 24.465059976937965 37.202050190357426 20.914272198076077 17.418617634628532 9 25.457631776211322 17.903511655370444 21.634656356581463 35.004200211836775 10-11 22.940903311981607 29.287636115087146 28.77961226005395 18.9918483128773 12-13 24.51041094175021 26.867308321100854 28.554086112019366 20.06819462512957 14-15 21.310391966231457 29.183196428354023 27.07857586356229 22.427835741852224 16-17 20.248516017273854 31.786623024037652 27.837828906748662 20.127032051939832 18-19 20.14559412962243 30.633119664251694 29.996199699280552 19.225086506845322 20-21 21.639672152531688 29.787855729721503 29.711068113137813 18.861404004608996 22-23 20.885241567864927 30.043497694674357 29.47103603125712 19.600224706203594 24-25 20.501530594496817 30.0890527707239 29.878856924200786 19.530559710578498 26-27 20.746236985553224 30.2636110328418 29.55640434272913 19.433747638875847 28-29 20.300978126731202 30.737895605604553 29.50634473510046 19.454781532563782 30-31 20.959694367510544 30.31046888700227 29.40051286690512 19.329323878582066 32-33 20.74179586917219 30.43161419866619 29.34146941707343 19.48512051508819 34-35 20.948040156954416 30.62889603161351 29.272850921133188 19.15021289029888 36-37 20.802794324723703 30.28956503058467 29.4519982013622 19.45564244332943 38-39 20.598640852015677 30.66466754447148 29.35309147897356 19.38360012453928 40-41 21.208418489368178 30.452754707135714 29.132582510762273 19.206244292733835 42-43 20.815605920926753 30.60652971769494 28.93939921031118 19.638465151067123 44-45 21.054856026736214 30.714122346184585 28.59333107806672 19.63769054901248 46-47 21.092932005468228 30.525881890503303 28.405292212648575 19.975893891379894 48-49 21.02624530402115 30.552734103241963 28.462762626965354 19.95825796577153 50-51 20.87270652773212 30.984682066346984 28.255126124668884 19.887485281252008 52-53 21.276469923210044 30.953743401542756 27.875014131786518 19.89477254346068 54-55 21.13587328476751 30.948233655124675 27.911057928528756 20.004835131579064 56-57 21.137703044791383 31.045601866546775 27.714010666726963 20.10268442193488 58-59 21.201395912269366 31.110877280249476 27.600573600630995 20.08715320685016 60-61 21.181081546437834 30.9013640640517 27.98653154148108 19.931022848029386 62-63 21.011613598911254 30.70129440973264 28.11334455126115 20.17374744009496 64-65 20.98686836797792 30.891557714735974 28.057807359245242 20.063766558040875 66-67 21.08870690727199 30.57204202331788 27.8589274331635 20.480323636246634 68-69 21.01434529586303 30.69089783324811 27.971761612071756 20.3229952588171 70-71 21.19441717113828 30.476144094660068 27.805350631325098 20.524088102876554 72-73 20.991520905503627 30.475512907375307 27.93343706431466 20.599529122806405 74-75 20.73616174547074 30.79594314418501 27.61093563020065 20.8569594801436 76-77 20.67055284562744 30.47412484878949 27.95213790572465 20.90318439985842 78-79 20.806317647928545 30.63807026469936 27.43576028770346 21.119851799668638 80-81 20.824528541439484 30.355791857328455 27.467211389791835 21.35246821144023 82-83 20.715514437434884 30.483590147653018 27.56966512386747 21.231230291044632 84-85 20.75329278621978 30.52738495466712 27.300950138181364 21.418372120931735 86-87 20.530094456609266 30.31255380687441 27.295194730883015 21.86215700563331 88-89 20.388337694479734 30.351230991184657 27.477918163500814 21.78251315083479 90-91 20.277738403434643 30.315297178700284 27.621570337698525 21.785394080166547 92-93 20.093309216133015 30.544637111501473 27.486999321703742 21.875054350661774 94-95 19.82251170718345 31.09688808497872 27.220929878645318 21.85967032919252 96-97 19.673930246674168 31.351081536569797 27.1460722230242 21.828915993731837 98-99 19.81176458311412 31.387033966031357 26.716106798336963 22.085094652517558 100-101 19.62300182794371 31.59290249981303 26.116376184206636 22.66771948803662 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 361.0 1 225.5 2 86.5 3 72.0 4 66.5 5 79.5 6 129.0 7 202.5 8 250.0 9 255.5 10 257.5 11 296.5 12 343.0 13 395.5 14 420.0 15 457.0 16 528.5 17 601.5 18 683.5 19 805.5 20 1037.0 21 1342.0 22 1697.0 23 2167.5 24 2877.0 25 3783.0 26 4999.5 27 6657.0 28 7883.5 29 9115.0 30 10533.0 31 11890.5 32 13773.0 33 15767.0 34 17869.5 35 19935.0 36 21940.5 37 23285.0 38 23286.0 39 23089.0 40 23218.0 41 23152.0 42 22810.0 43 22691.5 44 22668.0 45 22229.5 46 21784.0 47 21620.0 48 21746.5 49 20826.5 50 18596.5 51 16572.0 52 14756.0 53 12862.5 54 11163.5 55 9653.0 56 8075.0 57 6735.5 58 5672.5 59 4860.5 60 4080.5 61 3153.5 62 2266.0 63 1470.0 64 923.5 65 609.0 66 442.5 67 329.5 68 244.5 69 169.5 70 109.5 71 76.0 72 57.5 73 35.0 74 20.0 75 13.0 76 9.5 77 4.5 78 1.5 79 1.0 80 0.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02713170879328247 2 0.00834821809024076 3 0.01043527261280095 4 0.0 5 0.005391557516613824 6 0.0 7 0.0 8 3.478424204266983E-4 9 3.478424204266983E-4 10-11 0.0 12-13 1.7392121021334915E-4 14-15 0.0 16-17 0.0 18-19 0.0032175423889469587 20-21 0.0018261727072401662 22-23 0.0 24-25 0.0057393999370405225 26-27 0.00608724235746722 28-29 0.008261257485134085 30-31 0.02869699968520261 32-33 6.08724235746722E-4 34-35 0.005043715096187125 36-37 0.016261633154948146 38-39 0.009391745351520854 40-41 0.00834821809024076 42-43 0.010957036243440996 44-45 0.003913227229800356 46-47 0.0026088181532002374 48-49 0.0022609757327735386 50-51 0.004521951465547077 52-53 0.004869793885973776 54-55 0.0025218575480935625 56-57 0.0013044090766001187 58-59 0.0014783302868134679 60-61 0.0 62-63 8.696060510667457E-5 64-65 0.011043996848547672 66-67 0.0011304878663867693 68-69 0.0019131333123468407 70-71 0.005478518121720498 72-73 0.015392027103881399 74-75 0.007826454459600712 76-77 0.0051306757012938 78-79 0.013131051371107861 80-81 3.478424204266983E-4 82-83 0.007652533249387363 84-85 6.956848408533966E-4 86-87 0.0 88-89 0.0016522514970268168 90-91 0.0013044090766001187 92-93 5.217636306400475E-4 94-95 9.565666561734203E-4 96-97 6.956848408533966E-4 98-99 2.6088181532002376E-4 100-101 0.0017392121021334913 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 574973.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.87307454057844 #Duplication Level Percentage of deduplicated Percentage of total 1 86.8986360249664 68.53962596671768 2 9.430727951653653 14.8766101740539 3 2.13694246482843 5.056416669520205 4 0.7224780551764959 2.279362619994716 5 0.2940345799592642 1.1595705671317358 6 0.13952667200000635 0.6602938560633204 7 0.0844416016914763 0.466211812117635 8 0.04390535576438433 0.277036031834792 9 0.03617343436349845 0.25677989864468415 >10 0.18047598573718115 2.6487689510326877 >50 0.020521534142088668 1.0908982877771625 >100 0.011033036106592292 1.598574813346195 >500 2.2066072212371363E-4 0.09694133598422532 >1k 8.826428884948545E-4 0.9929090157809792 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2072 0.36036474756205944 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1571 0.27323022124517155 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1042 0.1812259010423098 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1020 0.1773996344176161 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 8.696060510667457E-5 0.0 0.0 0.0 14-15 0.0 2.608818153200237E-4 0.0 0.0 0.0 16-17 0.0 3.478424204266983E-4 0.0 0.0 0.0 18-19 0.0 3.478424204266983E-4 0.0 0.0 0.0 20-21 0.0 3.478424204266983E-4 0.0 1.7392121021334915E-4 0.0 22-23 0.0 3.478424204266983E-4 0.0 7.826454459600712E-4 0.0 24-25 0.0 3.478424204266983E-4 0.0 0.001217448471493444 0.0 26-27 0.0 3.478424204266983E-4 0.0 0.0020870545225601896 0.0 28-29 0.0 3.478424204266983E-4 0.0 0.005478518121720498 0.0 30-31 0.0 3.478424204266983E-4 0.0 0.011565760479187719 0.0 32-33 0.0 3.478424204266983E-4 0.0 0.018522608887721684 0.0 34-35 0.0 5.217636306400474E-4 0.0 0.024783772455402253 0.0 36-37 0.0 5.217636306400474E-4 0.0 0.03165366025882955 0.0 38-39 0.0 5.217636306400474E-4 0.0 0.04269765710737722 0.0 40-41 0.0 6.08724235746722E-4 0.0 0.06852495682405957 0.0 42-43 0.0 6.956848408533966E-4 0.0 0.11817946233997076 0.0 44-45 0.0 6.956848408533966E-4 0.0 0.16131192247288134 0.0 46-47 0.0 6.956848408533966E-4 0.0 0.27488247274219835 0.0 48-49 0.0 6.956848408533966E-4 0.0 0.3748871686148741 0.0 50-51 0.0 7.826454459600712E-4 0.0 0.43506390734869294 0.0 52-53 0.0 8.696060510667458E-4 0.0 0.5197635367225939 0.0 54-55 0.0 8.696060510667458E-4 0.0 0.5993324903952011 0.0 56-57 0.0 8.696060510667458E-4 0.0 0.7748189915004704 0.0 58-59 0.0 8.696060510667458E-4 0.0 0.9846549316228762 0.0 60-61 0.0 8.696060510667458E-4 0.0 1.1852730476039746 0.0 62-63 0.0 8.696060510667458E-4 0.0 1.4177187450541155 0.0 64-65 0.0 8.696060510667458E-4 0.0 1.7130369599963826 0.0 66-67 0.0 8.696060510667458E-4 0.0 2.086706680139763 0.0 68-69 0.0 8.696060510667458E-4 0.0 2.4847253697130123 0.0 70-71 0.0 8.696060510667458E-4 0.0 2.8049143177157885 0.0 72-73 0.0 8.696060510667458E-4 0.0 3.1082329083278695 0.0 74-75 0.0 8.696060510667458E-4 0.0 3.4377266410770595 0.0 76-77 0.0 8.696060510667458E-4 0.0 3.8530504910665373 0.0 78-79 0.0 8.696060510667458E-4 0.0 4.2616783744628 0.0 80-81 0.0 8.696060510667458E-4 0.0 4.756745099335099 0.0 82-83 0.0 8.696060510667458E-4 0.0 5.242159197040556 0.0 84-85 0.0 8.696060510667458E-4 0.0 6.0221088642423215 0.0 86-87 0.0 8.696060510667458E-4 0.0 6.782405434689977 0.0 88-89 0.0 8.696060510667458E-4 0.0 7.608705104413598 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTGCG 60 1.4923844E-6 47.493473 9 GGTATCA 2265 0.0 27.487757 1 GTATCAA 3265 0.0 27.074842 1 ATCAACG 3735 0.0 23.532398 3 GAGTACT 2035 0.0 23.45705 12-13 TCAACGC 3760 0.0 23.367802 4 TATCAAC 3795 0.0 23.160345 2 CAACGCA 3805 0.0 23.091442 5 GTATAGG 165 2.4146193E-6 23.043173 1 GCATGCG 105 0.0021966486 22.615942 9 AACGCAG 3900 0.0 22.528957 6 AGAGTAC 3205 0.0 21.635094 10-11 GTGTATC 200 5.3476106E-7 21.386946 1 GTACTTT 2285 0.0 21.101252 14-15 AGTACTT 2140 0.0 20.863434 12-13 ACGCAGA 4300 0.0 20.43324 7 CGCAGAG 4295 0.0 20.346449 8 GTACATG 2110 0.0 20.271986 1 CTAGACT 245 1.7547427E-7 19.385096 4 GCAGAGT 4485 0.0 19.272715 9 >>END_MODULE