FastQCFastQC Report
Thu 26 May 2016
SRR937073_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937073_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621994
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41200.6623858107956025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30320.48746450930394825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21220.3411608472107448No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7220.11607829014427791No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27200.040.3506281
GGTATCA15300.040.0594941
TACATCG550.002575412434.5544935
GACTTCG550.002576428534.5517127
ATCAACG33450.032.669233
TCAACGC33850.032.2831844
TATCAAC34150.032.1387142
CAACGCA34250.031.9061575
ACTACCC902.4082112E-531.6724038
AACGCAG34850.031.3543176
ACGCAGA38500.028.3817637
CGCAGAG38350.028.2450128
GCGTAGA700.0083812527.1477746
GCAGAGT40300.025.8173319
AGAGTAC34600.024.09757410-11
AGTACTT30400.022.19062812-13
GTACATG17400.021.8447931
TACATGG16400.021.7282432
GAGTACT29350.020.96121812-13
CTTCGGC1150.00371747920.6559169
Produced by FastQC (version 0.11.5)