##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937073_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 621994 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.044378241590756 33.0 31.0 34.0 30.0 34.0 2 32.179328096412505 33.0 31.0 34.0 30.0 34.0 3 32.19122853275112 34.0 31.0 34.0 30.0 34.0 4 35.744119718196636 37.0 35.0 37.0 35.0 37.0 5 35.742225809252176 37.0 35.0 37.0 35.0 37.0 6 35.832196130509296 37.0 35.0 37.0 35.0 37.0 7 35.78516673794281 37.0 35.0 37.0 35.0 37.0 8 35.75540117750332 37.0 35.0 37.0 35.0 37.0 9 37.42770348267025 39.0 37.0 39.0 35.0 39.0 10-11 37.33245497544992 39.0 37.0 39.0 34.0 39.0 12-13 37.266440673061155 39.0 37.0 39.0 34.0 39.0 14-15 38.514295957838826 40.0 38.0 41.0 34.0 41.0 16-17 38.452428801564 40.0 38.0 41.0 34.0 41.0 18-19 38.436189095071654 40.0 38.0 41.0 34.0 41.0 20-21 38.361163451737475 40.0 38.0 41.0 34.0 41.0 22-23 38.24376923250064 40.0 38.0 41.0 34.0 41.0 24-25 38.34534577503963 40.0 38.0 41.0 34.0 41.0 26-27 38.28691273549262 40.0 38.0 41.0 34.0 41.0 28-29 38.20160403476561 40.0 38.0 41.0 34.0 41.0 30-31 38.00954028495452 40.0 38.0 41.0 33.5 41.0 32-33 37.759789483499844 40.0 37.0 41.0 33.0 41.0 34-35 37.561138853429455 40.0 37.0 41.0 33.0 41.0 36-37 37.3890286723023 40.0 37.0 41.0 32.5 41.0 38-39 37.23594198657865 40.0 37.0 41.0 32.0 41.0 40-41 37.05118940054084 40.0 36.0 41.0 31.5 41.0 42-43 36.78799956269675 40.0 36.0 41.0 31.0 41.0 44-45 36.46649404978183 39.0 36.0 41.0 30.0 41.0 46-47 36.05612674720335 39.0 36.0 41.0 29.5 41.0 48-49 35.73104081389853 39.0 35.5 41.0 27.5 41.0 50-51 35.01008852175423 38.5 35.0 40.0 26.0 40.5 52-53 35.18503152763532 38.5 35.5 40.0 27.0 40.5 54-55 35.601994553002115 39.5 36.0 41.0 27.0 41.0 56-57 35.54192243011991 39.5 36.0 41.0 26.5 41.0 58-59 35.35050740039293 39.0 35.5 41.0 26.0 41.0 60-61 35.16123547815574 39.0 35.0 41.0 26.0 41.0 62-63 34.890365501918026 39.0 35.0 41.0 24.5 41.0 64-65 34.626895918610145 39.0 35.0 40.5 23.0 41.0 66-67 34.32123300224761 38.0 34.0 40.0 22.5 41.0 68-69 33.97028267153702 38.0 34.0 40.0 22.0 41.0 70-71 33.641720016591805 37.0 34.0 40.0 20.5 41.0 72-73 33.261417312707195 37.0 34.0 39.0 19.5 41.0 74-75 32.83163744344801 36.0 33.0 39.0 18.0 40.5 76-77 32.415059470027046 36.0 33.0 39.0 15.5 40.0 78-79 31.980093859426297 35.0 32.0 37.5 12.0 39.5 80-81 31.518751788602465 35.0 32.0 37.0 7.5 39.0 82-83 31.080025691566156 35.0 31.5 36.5 7.0 39.0 84-85 30.654054540719045 35.0 31.0 36.0 2.0 37.5 86-87 30.270066109962478 35.0 31.0 36.0 2.0 37.0 88-89 29.9005271755033 34.0 31.0 35.0 2.0 37.0 90-91 29.5120210162799 34.0 30.0 35.0 2.0 36.0 92-93 29.18063518297604 34.0 30.0 35.0 2.0 36.0 94-95 28.835193747849658 34.0 30.0 35.0 2.0 36.0 96-97 28.40728688701177 34.0 29.0 35.0 2.0 35.0 98-99 27.86418277346727 34.0 29.0 35.0 2.0 35.0 100-101 25.65245163136622 32.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3110278662029806 1101 2 0.3868545888566217 1101 3 0.20414850396549156 1101 4 0.3076948513173363 1101 5 0.33267811254403057 1101 6 0.0589128516841555 1101 7 0.2487211965087397 1101 8 0.19704396682258363 1101 9 0.2781236216431253 1101 10-11 0.3799385856508053 1101 12-13 0.4457256652794044 1101 14-15 0.5037161969701884 1101 16-17 0.648883476684702 1101 18-19 0.670351903251138 1101 20-21 0.6326339611040481 1101 22-23 0.5440795728194558 1101 24-25 0.5640074749863757 1101 26-27 0.669570978496111 1101 28-29 0.5640826806446526 1101 30-31 0.6277539949639603 1101 32-33 0.6893309727317813 1101 34-35 0.8311418621787894 1101 36-37 0.8950206517630974 1101 38-39 0.9779906942369436 1101 40-41 1.0474089374381563 1101 42-43 1.0601246575633851 1101 44-45 1.2456258973196341 1101 46-47 1.20724947845396 1101 48-49 1.3763297229889844 1101 50-51 1.3870290020029117 1101 52-53 1.4504085405603746 1101 54-55 1.4892771639369116 1101 56-57 1.4648620168051067 1101 58-59 1.2981014621171383 1101 60-61 1.519024078353084 1101 62-63 1.6403096645022615 1101 64-65 1.5692053013764422 1101 66-67 1.2694099393408393 1101 68-69 1.3376186677151551 1101 70-71 1.2008223070550414 1101 72-73 1.1468033833179518 1101 74-75 1.2119526469684416 1101 76-77 1.0788882363830865 1101 78-79 1.1757087683204013 1101 80-81 1.2030783671569019 1101 82-83 1.1568569990601247 1101 84-85 1.1909279891436526 1101 86-87 1.1858417066636058 1101 88-89 1.2177925122077227 1101 90-91 1.011661639036877 1101 92-93 1.0828692492518002 1101 94-95 1.0956474837651804 1101 96-97 0.8591537644123193 1101 98-99 0.6290021154881238 1101 100-101 0.8730218221713173 1102 1 0.2032055146591638 1102 2 0.2293105270196918 1102 3 0.22901145909473541 1102 4 0.11770017687345558 1102 5 0.12907303024990568 1102 6 -0.02935362100010508 1102 7 0.14001433225330118 1102 8 0.08423121639965103 1102 9 0.24496321488487638 1102 10-11 0.311632868489923 1102 12-13 0.3221580634764436 1102 14-15 0.4440187288773245 1102 16-17 0.43065033492578664 1102 18-19 0.32308238475197015 1102 20-21 0.344028299214699 1102 22-23 0.42750533339119556 1102 24-25 0.14458633101659757 1102 26-27 0.3231939526428107 1102 28-29 0.1169043853938021 1102 30-31 0.38172632223197667 1102 32-33 0.42422369721573716 1102 34-35 0.4399033259843321 1102 36-37 0.4637211656708544 1102 38-39 0.5586140699003295 1102 40-41 0.5625670325354548 1102 42-43 0.6953278434891388 1102 44-45 0.5932914725552934 1102 46-47 0.6913555494199315 1102 48-49 0.7745087019942005 1102 50-51 1.0797393740694474 1102 52-53 1.1932664994107967 1102 54-55 1.2830374433522067 1102 56-57 1.485345248023286 1102 58-59 1.298235441013155 1102 60-61 1.3111354513553692 1102 62-63 1.4463708025584765 1102 64-65 1.4055053502640433 1102 66-67 1.2652603735332661 1102 68-69 1.429840247809338 1102 70-71 1.5002878194592242 1102 72-73 1.2766196936315595 1102 74-75 1.3130336340611066 1102 76-77 1.3691873243185952 1102 78-79 1.5979984886531078 1102 80-81 1.3360782512378542 1102 82-83 1.241920578136238 1102 84-85 1.090054900337904 1102 86-87 1.1631866733410767 1102 88-89 1.4160354986855133 1102 90-91 1.397399480557059 1102 92-93 1.0937269997839358 1102 94-95 1.3986178672892216 1102 96-97 1.2937985229395075 1102 98-99 1.1076184116898418 1102 100-101 1.38458096692397 1103 1 0.35909437448278325 1103 2 0.29989451584241067 1103 3 0.3304211921741924 1103 4 0.35001717214662875 1103 5 0.4160567246347 1103 6 0.21869714723547418 1103 7 0.10095264020859673 1103 8 0.2237708631147015 1103 9 0.4369987907726056 1103 10-11 0.38273699041803866 1103 12-13 0.39000349573442605 1103 14-15 0.4715878112733023 1103 16-17 0.4293631829232254 1103 18-19 0.5072196431131388 1103 20-21 0.4211448759480163 1103 22-23 0.7146588568113756 1103 24-25 0.4284624075298993 1103 26-27 0.5273193280410169 1103 28-29 0.6288623772947304 1103 30-31 0.7926618926446238 1103 32-33 0.7821959283941169 1103 34-35 0.6417479227848517 1103 36-37 0.7878502722383232 1103 38-39 0.863714213266455 1103 40-41 0.818757427613555 1103 42-43 0.7632087624112316 1103 44-45 0.9101387156470508 1103 46-47 0.9838751634061396 1103 48-49 1.181681672106322 1103 50-51 1.0484051566112242 1103 52-53 0.8767781785789097 1103 54-55 1.0854137317715882 1103 56-57 1.1309409474451542 1103 58-59 1.1243837387685787 1103 60-61 1.292809631055782 1103 62-63 1.4504416298844305 1103 64-65 1.3411583961351212 1103 66-67 1.2659128212005797 1103 68-69 1.248389329921416 1103 70-71 1.1144073547664206 1103 72-73 1.083841939511558 1103 74-75 1.1750477962818024 1103 76-77 1.0139141074220746 1103 78-79 1.051228586211014 1103 80-81 1.2214340202541472 1103 82-83 1.1586060020396474 1103 84-85 1.2468765524753174 1103 86-87 1.1242927825223923 1103 88-89 1.2162841081152322 1103 90-91 1.0989049262391468 1103 92-93 1.4643010523406979 1103 94-95 1.4114827969343757 1103 96-97 1.092301188494936 1103 98-99 1.1097506532506038 1103 100-101 1.1326804025846187 1104 1 0.34484726075151073 1104 2 0.35270709260487365 1104 3 0.3842514199174616 1104 4 0.3440165405011939 1104 5 0.4290756389063759 1104 6 0.3350602731785557 1104 7 0.3483997399033001 1104 8 0.45986589066146166 1104 9 0.36214880656373793 1104 10-11 0.48913415503186286 1104 12-13 0.32023299357630464 1104 14-15 0.4363297139297231 1104 16-17 0.475885623881922 1104 18-19 0.42743931535933655 1104 20-21 0.5286298743689031 1104 22-23 0.5613620185476975 1104 24-25 0.500215574529939 1104 26-27 0.4215011717438628 1104 28-29 0.44680812597002983 1104 30-31 0.4171749992874183 1104 32-33 0.5167557066163866 1104 34-35 0.5432814175386795 1104 36-37 0.5843727131970198 1104 38-39 0.26859016512103295 1104 40-41 0.3620075942977721 1104 42-43 0.6137017967657314 1104 44-45 0.3947335852824594 1104 46-47 0.4714440303044114 1104 48-49 0.5242703656530097 1104 50-51 0.5194284222181338 1104 52-53 0.5586218814248198 1104 54-55 0.4954218027966064 1104 56-57 0.6379065929517012 1104 58-59 0.8614437801764439 1104 60-61 0.7994243624310116 1104 62-63 0.7332257826022541 1104 64-65 0.720417616403914 1104 66-67 0.7237768068832793 1104 68-69 0.4850370472249992 1104 70-71 0.3937876404105296 1104 72-73 0.4509858493968082 1104 74-75 0.36206748256456933 1104 76-77 0.265449356746565 1104 78-79 0.5233221399184238 1104 80-81 0.40633944889575346 1104 82-83 0.23660544057703703 1104 84-85 0.42673759047931625 1104 86-87 0.4856290635344287 1104 88-89 0.5090429950156086 1104 90-91 0.5499436320678264 1104 92-93 0.48152216333484077 1104 94-95 0.6123723642572507 1104 96-97 0.6063991637203969 1104 98-99 0.49665980158200895 1104 100-101 0.5973696681213383 1105 1 0.24365045268994479 1105 2 0.2767043774985538 1105 3 0.25105755659807727 1105 4 0.26830593571463623 1105 5 0.10180739383088167 1105 6 0.2921268101685044 1105 7 0.07973760516784267 1105 8 0.16382474531884839 1105 9 0.13505920467802213 1105 10-11 0.20829118881196962 1105 12-13 0.3660596995512222 1105 14-15 0.33737607673412384 1105 16-17 0.4586713770031139 1105 18-19 0.3221056848501931 1105 20-21 0.27899374589439674 1105 22-23 0.21379041774937946 1105 24-25 0.13735123866722887 1105 26-27 0.15241353527093793 1105 28-29 0.056060762561152444 1105 30-31 -0.11277137398566595 1105 32-33 0.22963912251731955 1105 34-35 -0.18077648446759298 1105 36-37 0.058092915411656065 1105 38-39 0.13076897040671298 1105 40-41 2.7201998862125265E-4 1105 42-43 0.19448296392375397 1105 44-45 -0.0123601365407211 1105 46-47 0.12087196685737212 1105 48-49 -0.08574125121982945 1105 50-51 -0.09488295725174822 1105 52-53 -0.026125569786003666 1105 54-55 0.13796404059804956 1105 56-57 0.24894903843851068 1105 58-59 0.06312738248765726 1105 60-61 4.055491756176366E-4 1105 62-63 -0.03516956929781401 1105 64-65 0.10917255104212131 1105 66-67 0.1358093881591813 1105 68-69 0.1416926035685222 1105 70-71 0.09252312354073666 1105 72-73 -0.05007959344580826 1105 74-75 -0.11444127567952478 1105 76-77 -0.06619885462648512 1105 78-79 -0.01207217524328641 1105 80-81 0.15809211081436203 1105 82-83 0.1010941124375293 1105 84-85 -0.07239283255720963 1105 86-87 -0.3452382170704631 1105 88-89 -0.3317594134593058 1105 90-91 -0.3297617924039251 1105 92-93 -0.6688884796117947 1105 94-95 -0.5652729226356037 1105 96-97 -0.5438206788439608 1105 98-99 -0.44647915175125874 1105 100-101 -0.23701475581346898 1106 1 -0.05199145459702237 1106 2 0.0868387382149578 1106 3 0.05091808658412589 1106 4 0.07910312422009724 1106 5 0.22099902010648265 1106 6 0.17934942364323803 1106 7 0.16343342244831405 1106 8 0.10973523594950052 1106 9 0.16050902999528205 1106 10-11 0.18021767016303158 1106 12-13 0.22655516332354608 1106 14-15 0.22813722397854974 1106 16-17 0.15076715561863097 1106 18-19 0.1567543170774286 1106 20-21 0.3819150212855362 1106 22-23 0.2684737103464201 1106 24-25 0.22073635542158598 1106 26-27 0.1688081663658494 1106 28-29 0.3507429504452162 1106 30-31 0.3769996742488644 1106 32-33 -0.04337342978393366 1106 34-35 0.19125287327989327 1106 36-37 0.027406060115247044 1106 38-39 -0.1627601733026367 1106 40-41 -0.11440063974597336 1106 42-43 -0.007859027597582724 1106 44-45 -0.011842302330521193 1106 46-47 -0.031171061713266113 1106 48-49 -0.014898079036498757 1106 50-51 -0.09087492046480605 1106 52-53 -0.026415556943710783 1106 54-55 -0.15786773923264974 1106 56-57 -0.06317380564505726 1106 58-59 -0.07313896381224794 1106 60-61 0.029808175630734013 1106 62-63 0.019261710650262387 1106 64-65 -0.06647336082939859 1106 66-67 0.1270683466910114 1106 68-69 -0.027311221500177396 1106 70-71 0.015945800536464105 1106 72-73 -0.1761307968925152 1106 74-75 0.12957601307864053 1106 76-77 -0.0647454666098568 1106 78-79 -0.08583248014406664 1106 80-81 -0.10278240759982182 1106 82-83 -0.002828309093331427 1106 84-85 0.0035182104299344985 1106 86-87 0.07498769673430772 1106 88-89 0.1907629224562939 1106 90-91 0.3208623704307705 1106 92-93 0.01982756772515515 1106 94-95 -0.050604405574691924 1106 96-97 0.04482563348601687 1106 98-99 0.0764091203395516 1106 100-101 -0.11307631594437595 1107 1 0.1997634163215416 1107 2 0.409257528317319 1107 3 0.3309519275316717 1107 4 0.25382795710947903 1107 5 0.1659069626773899 1107 6 0.19154556711545467 1107 7 0.10677496472436587 1107 8 0.3133337683915727 1107 9 0.45470748537381667 1107 10-11 0.2746456408788873 1107 12-13 0.22860739900396965 1107 14-15 0.24402320343944695 1107 16-17 0.3277536359925577 1107 18-19 0.30022234408317416 1107 20-21 0.5757675283636559 1107 22-23 0.4407442368914616 1107 24-25 0.49388473624951246 1107 26-27 0.47677335374752516 1107 28-29 0.3294750876183272 1107 30-31 0.24226830771119268 1107 32-33 0.3546807157321439 1107 34-35 0.34014047777638723 1107 36-37 0.5910630603330347 1107 38-39 0.30349939681681093 1107 40-41 0.6449810090788048 1107 42-43 0.8108908676965214 1107 44-45 0.926917309671289 1107 46-47 0.8524629869707852 1107 48-49 0.7500204178893313 1107 50-51 0.7529248148386785 1107 52-53 0.925679398744748 1107 54-55 0.7566126681989473 1107 56-57 0.47796304915717513 1107 58-59 0.6780379305269335 1107 60-61 0.9494317369716399 1107 62-63 0.8554137268671198 1107 64-65 0.7904941887174388 1107 66-67 0.6754592263881207 1107 68-69 0.9635709885482342 1107 70-71 0.791565240653064 1107 72-73 0.9331025883749433 1107 74-75 0.8815905546343217 1107 76-77 0.7515626870481427 1107 78-79 0.8751164066227766 1107 80-81 0.6422335914620163 1107 82-83 0.6885531386961219 1107 84-85 0.5196219781671125 1107 86-87 1.022877663395949 1107 88-89 0.8984156674262245 1107 90-91 0.8811352758939037 1107 92-93 0.5703167536995899 1107 94-95 0.7271468467078925 1107 96-97 0.6947226028783895 1107 98-99 0.5609545291951576 1107 100-101 0.6765527738681563 1108 1 -0.15625133612056175 1108 2 0.06387718616138471 1108 3 -0.019559832351141893 1108 4 0.08145408526901576 1108 5 0.17613908946731982 1108 6 0.1268915462765534 1108 7 -0.04064197601712749 1108 8 0.24439588894765052 1108 9 0.3417456654555622 1108 10-11 0.18105023980838553 1108 12-13 0.20074849917255477 1108 14-15 0.21317942148323965 1108 16-17 0.17320460807530935 1108 18-19 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68-69 -0.8546435606885652 2316 70-71 -1.0606813893278755 2316 72-73 -1.6446663982635137 2316 74-75 -1.6663468366494172 2316 76-77 -2.0171291025216114 2316 78-79 -1.8085729678530846 2316 80-81 -1.797685391768642 2316 82-83 -1.2198481284132647 2316 84-85 -1.1210461286659736 2316 86-87 -1.52108889866604 2316 88-89 -1.4157406350798425 2316 90-91 -1.639237448657969 2316 92-93 -1.934420089965304 2316 94-95 -1.7429237688595336 2316 96-97 -1.5499649313848316 2316 98-99 -1.5289240049169344 2316 100-101 -1.774666774464837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1005.0 3 315.0 4 285.0 5 308.0 6 360.0 7 402.0 8 506.0 9 644.0 10 908.0 11 1335.0 12 2074.0 13 2669.0 14 3062.0 15 4183.0 16 5738.0 17 8345.0 18 7397.0 19 3858.0 20 3278.0 21 3082.0 22 3113.0 23 3369.0 24 3766.0 25 4345.0 26 4938.0 27 5766.0 28 6901.0 29 8320.0 30 10570.0 31 12936.0 32 16787.0 33 22329.0 34 31395.0 35 45210.0 36 70916.0 37 121729.0 38 157355.0 39 42463.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.068981931159584 20.032355381735613 16.19232476312387 22.706337923980936 2 13.84085270457787 20.70194733465503 43.222918445412404 22.234281515354695 3 16.984263021557748 25.433864331069877 33.00520492482043 24.57666772255194 4 11.026096241549437 17.882711641998416 41.955802988492444 29.13538912795971 5 11.799886434377246 36.55880077276098 37.85869283716202 13.782619955699756 6 27.662705714322477 38.23246522639835 20.307011336001132 13.797817723278047 7 24.353216430690562 33.42675896668999 24.792686750765817 17.42733785185364 8 23.018474613892472 34.385203581044784 23.747744154102023 18.848577650960724 9 24.065357059575597 16.43430923988646 24.025312995022635 35.475020705515306 10-11 21.71774267469217 26.59310670884183 31.89180366357857 19.79734695288743 12-13 23.298752033504268 24.624560630392356 31.386208599139714 20.690478736963662 14-15 20.44294001535697 26.10355099759997 30.57612755128705 22.877381435756007 16-17 18.91275840138677 28.795508432944484 31.440245772271503 20.851487393397242 18-19 18.563438010788 27.361393290984243 34.39715257169825 19.6780161265295 20-21 19.861731182199197 26.385080024344575 34.434869019278686 19.318319774177546 22-23 19.04440333694297 26.514901201309115 34.38310208353934 20.057593378208576 24-25 18.94572511744713 26.384043268133162 34.620616030688986 20.049615583730716 26-27 18.94857506664051 26.660868111267956 34.32155294102515 20.069003881066376 28-29 18.79031966333458 26.966835883646244 34.339470965823 19.903373487196184 30-31 19.220362254298273 26.63281317826217 34.27959112145776 19.867233445981793 32-33 18.820288396455897 26.55761900091802 34.455080539750575 20.167012062875507 34-35 19.178400434730882 26.756126264883584 34.1865837934128 19.878889506972737 36-37 19.18925262944659 26.536670771743783 34.146712026161026 20.12736457264861 38-39 19.03905593716966 26.827172353364386 34.0582884841859 20.075483225280045 40-41 19.53477005788646 26.652850873843537 33.72752287604291 20.08485619222709 42-43 19.17889817176546 26.71595442717649 33.76506346127412 20.340083939783938 44-45 19.369430202590703 26.911396108959423 33.34064852819887 20.378525160250998 46-47 19.531393816816415 26.78605162272596 32.90388082833663 20.778673732120996 48-49 19.585441829618762 26.887517835969373 32.89197934042083 20.635060993991036 50-51 19.64536635401628 27.185873175625485 32.40087524960048 20.767885220757755 52-53 19.63394998163158 27.28357220315712 32.25253078217307 20.82994703303823 54-55 19.77653694158352 26.974333662388943 32.22520908965211 21.023920306375427 56-57 19.7842090962731 27.200634735872388 31.957134427990113 21.0580217398644 58-59 19.928953784589613 27.35415951555452 31.973771261411972 20.743115438443898 60-61 19.79869224721576 27.04318285226445 32.165268182570905 20.992856717948882 62-63 19.53112941812008 26.79385643360601 32.319882826567884 21.355131321706025 64-65 19.585539627276667 26.832044592392634 32.35601244072448 21.226403339606215 66-67 19.58369543458984 26.812187825840883 32.23522526382811 21.368891475741158 68-69 19.650237604642918 26.569271943357563 32.345022361607654 21.435468090391865 70-71 19.627003793318035 26.275280076799014 32.450557419964845 21.647158709918106 72-73 19.475048398196567 26.31471047909391 32.502202841504754 21.70803828120478 74-75 19.442097316609814 26.61651004110667 32.0410954168432 21.90029722544031 76-77 19.288395234848387 26.6623033281445 32.08211653008452 21.967184906922594 78-79 19.49070712966603 26.552275561322812 31.877958165400788 22.079059143610362 80-81 19.258726716091317 26.513278150781378 31.95103533629431 22.276959796832994 82-83 19.49136528730512 26.29500588191306 32.2135854099609 22.000043420820926 84-85 19.735946108694307 26.12400177836807 31.931997520575443 22.208054592362185 86-87 19.351934108075298 26.145330444464992 32.12970965265244 22.373025794807273 88-89 19.588577014787624 26.004881507671975 31.941556969413398 22.464984508127003 90-91 19.454322526180377 26.069827742155937 32.0276577355229 22.448191996140782 92-93 19.229096195331866 26.18144598133486 32.1162747877518 22.473183035581474 94-95 19.180052417474634 26.432717508401264 31.85205730548454 22.53517276863956 96-97 19.009631928476097 26.595137403720916 31.867934843782663 22.527295824020324 98-99 19.237059718438516 26.597091110260557 31.636909835353755 22.52893933594717 100-101 19.249602533492133 26.56025206252347 31.378663949606793 22.8114814543776 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 395.0 1 243.5 2 103.0 3 107.5 4 88.0 5 86.0 6 142.5 7 315.0 8 526.5 9 623.5 10 596.0 11 567.5 12 638.0 13 728.5 14 765.5 15 814.0 16 908.5 17 1023.0 18 1160.5 19 1366.5 20 1613.5 21 1925.5 22 2331.0 23 3088.5 24 3994.5 25 4834.0 26 6207.0 27 7908.5 28 9220.5 29 10492.5 30 11789.5 31 13334.5 32 15393.5 33 17316.5 34 19567.0 35 22114.5 36 24123.5 37 25002.5 38 24820.0 39 24344.0 40 24187.0 41 24038.0 42 23847.0 43 23943.0 44 23777.5 45 23454.0 46 23284.5 47 23192.5 48 23094.0 49 21935.5 50 19684.0 51 17526.5 52 15540.0 53 13487.5 54 11647.5 55 10033.5 56 8549.5 57 7174.5 58 5899.0 59 4776.5 60 3698.0 61 2727.0 62 1878.5 63 1249.5 64 870.0 65 619.5 66 429.5 67 321.5 68 242.0 69 150.5 70 94.5 71 74.5 72 46.5 73 33.0 74 23.0 75 10.0 76 8.0 77 5.0 78 4.0 79 3.0 80 2.0 81 1.5 82 2.0 83 3.0 84 1.5 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02411598825712145 2 0.010771808088180915 3 0.013344180168940536 4 0.02282980221674164 5 0.05257285440052477 6 0.041479499802248895 7 0.01752428480017492 8 0.04437341839310347 9 0.028778412653498266 10-11 0.022990575471789117 12-13 0.03577204924806349 14-15 0.020337816763505757 16-17 0.01889085746807847 18-19 0.015996938877223896 20-21 0.014389206326749133 22-23 0.007636729614755126 24-25 0.0019292790605697162 26-27 0.0 28-29 0.0018488924330459777 30-31 0.0 32-33 4.8231976514242906E-4 34-35 0.0 36-37 0.0 38-39 2.4115988257121453E-4 40-41 8.038662752373817E-5 42-43 8.038662752373817E-5 44-45 1.6077325504747634E-4 46-47 8.038662752373817E-5 48-49 0.0010450261578085962 50-51 0.0 52-53 4.019331376186909E-4 54-55 0.0019292790605697162 56-57 4.8231976514242906E-4 58-59 7.234796477136435E-4 60-61 0.0017685058055222397 62-63 0.0016077325504747636 64-65 7.234796477136435E-4 66-67 0.007315183104660174 68-69 0.009244462165229889 70-71 0.017845831310269872 72-73 0.011254127853323345 74-75 0.012138380756084463 76-77 0.023472895236931544 78-79 0.014871526091891563 80-81 0.03850519458387058 82-83 0.02773338649568967 84-85 0.012138380756084463 86-87 0.010691421460657177 88-89 0.0088425290276112 90-91 0.017122351662556233 92-93 0.022427869079122947 94-95 0.010289488323038486 96-97 0.01672041852493754 98-99 0.028296092888355835 100-101 0.039148287604060486 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 621994.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.90824870375232 #Duplication Level Percentage of deduplicated Percentage of total 1 88.04960757662221 70.35889940500523 2 8.970101370267184 14.335701823863594 3 1.7884710239606003 4.287407621462947 4 0.5472922943278421 1.7493267507518568 5 0.22767948973293275 0.9096734645161308 6 0.10224052215751107 0.49019166432983374 7 0.06556342113056085 0.3667340713098796 8 0.039174971725405794 0.25043227068769536 9 0.0301268407821052 0.21666447772873293 >10 0.1505812475048471 2.358462148064163 >50 0.01347455890828839 0.7927598550687432 >100 0.014480014966797862 2.1121396763362665 >500 6.03333956845673E-4 0.2812329326044175 >1k 6.03333956845673E-4 1.490373838270496 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4120 0.6623858107956025 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3032 0.48746450930394825 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2122 0.3411608472107448 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 722 0.11607829014427791 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.6077325504747634E-4 0.0 12-13 0.0 1.6077325504747634E-4 0.0 1.6077325504747634E-4 0.0 14-15 0.0 4.0193313761869085E-4 0.0 1.6077325504747634E-4 0.0 16-17 0.0 4.82319765142429E-4 0.0 1.6077325504747634E-4 0.0 18-19 0.0 4.82319765142429E-4 0.0 1.6077325504747634E-4 0.0 20-21 0.0 4.82319765142429E-4 0.0 1.6077325504747634E-4 0.0 22-23 0.0 4.82319765142429E-4 0.0 4.0193313761869085E-4 0.0 24-25 0.0 4.82319765142429E-4 0.0 0.0010450261578085962 0.0 26-27 0.0 4.82319765142429E-4 0.0 0.001929279060569716 0.0 28-29 0.0 4.82319765142429E-4 0.0 0.00570745055418541 0.0 30-31 0.0 4.82319765142429E-4 0.0 0.010209101695514748 0.0 32-33 0.0 4.82319765142429E-4 0.0 0.016398872014842587 0.0 34-35 0.0 4.82319765142429E-4 0.0 0.02114168303874314 0.0 36-37 0.0 4.82319765142429E-4 0.0 0.026125653945214905 0.0 38-39 0.0 4.82319765142429E-4 0.0 0.0360132091306347 0.0 40-41 0.0 5.627063926661672E-4 0.0 0.056833345659282886 0.0 42-43 0.0 6.430930201899054E-4 0.0 0.0968658861661045 0.0 44-45 0.0 6.430930201899054E-4 0.0 0.14332935687482518 0.0 46-47 0.0 6.430930201899054E-4 0.0 0.2418029755914044 0.0 48-49 0.0 6.430930201899054E-4 0.0 0.33577494316665435 0.0 50-51 0.0 6.430930201899054E-4 0.0 0.3937337016112695 0.0 52-53 0.0 6.430930201899054E-4 0.0 0.47331646285977036 0.0 54-55 0.0 6.430930201899054E-4 0.0 0.5505680119100828 0.0 56-57 0.0 6.430930201899054E-4 0.0 0.7201837959851702 0.0 58-59 0.0 6.430930201899054E-4 0.0 0.9168094869082339 0.0 60-61 0.0 6.430930201899054E-4 0.0 1.1047534220587338 0.0 62-63 0.0 6.430930201899054E-4 0.0 1.3221992495104455 0.0 64-65 0.0 6.430930201899054E-4 0.0 1.5902886523021122 0.0 66-67 0.0 6.430930201899054E-4 0.0 1.9472856651350336 0.0 68-69 0.0 6.430930201899054E-4 0.0 2.332980703993929 0.0 70-71 0.0 6.430930201899054E-4 0.0 2.630893545596903 0.0 72-73 0.0 6.430930201899054E-4 0.0 2.9110409425171304 0.0 74-75 0.0 6.430930201899054E-4 0.0 3.208632237610009 0.0 76-77 0.0 6.430930201899054E-4 0.0 3.5891021456798615 0.0 78-79 0.0 6.430930201899054E-4 0.0 3.990633350160934 0.0 80-81 0.0 6.430930201899054E-4 0.0 4.455268057248141 0.0 82-83 0.0 6.430930201899054E-4 0.0 4.913632607388496 0.0 84-85 0.0 6.430930201899054E-4 0.0 5.623607301678151 0.0 86-87 0.0 6.430930201899054E-4 0.0 6.375945748672817 0.0 88-89 0.0 6.430930201899054E-4 0.0 7.206902317385698 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2720 0.0 40.350628 1 GGTATCA 1530 0.0 40.059494 1 TACATCG 55 0.0025754124 34.554493 5 GACTTCG 55 0.0025764285 34.551712 7 ATCAACG 3345 0.0 32.66923 3 TCAACGC 3385 0.0 32.283184 4 TATCAAC 3415 0.0 32.138714 2 CAACGCA 3425 0.0 31.906157 5 ACTACCC 90 2.4082112E-5 31.672403 8 AACGCAG 3485 0.0 31.354317 6 ACGCAGA 3850 0.0 28.381763 7 CGCAGAG 3835 0.0 28.245012 8 GCGTAGA 70 0.00838125 27.147774 6 GCAGAGT 4030 0.0 25.817331 9 AGAGTAC 3460 0.0 24.097574 10-11 AGTACTT 3040 0.0 22.190628 12-13 GTACATG 1740 0.0 21.844793 1 TACATGG 1640 0.0 21.728243 2 GAGTACT 2935 0.0 20.961218 12-13 CTTCGGC 115 0.003717479 20.655916 9 >>END_MODULE