FastQCFastQC Report
Thu 26 May 2016
SRR937073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937073_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621994
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20410.3281382135518992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17860.28714103351479275No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11500.1848892433045978No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10670.17154506313565726No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG753.0713243E-431.6763441
GGTATCA26850.029.1988641
CATCCGA700.00837945227.148976
CCGATTG350.00839907627.13805614-15
GTATAAT4050.024.6371571
GTATCAA35650.023.8572181
GAGTACT22800.020.93379812-13
TACATGG21450.020.8138432
GTACATG21550.020.5051061
TAGGACG1656.325351E-520.1479514
TCAACGC40550.020.1444034
CAACGCA40600.020.1276865
ATCAACG40750.020.0471463
TATCAAC41900.019.7236332
CGCAGAA1456.1323826E-419.6595979
AACGCAG41650.019.6202646
GTACTTT24150.019.56693314-15
GGACGTG1707.932628E-519.5632296
AGTACTT23150.019.3864212-13
TATAATG4750.018.9981672
Produced by FastQC (version 0.11.5)