##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937073_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 621994 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24554899243401 33.0 31.0 34.0 31.0 34.0 2 32.368286510802356 34.0 31.0 34.0 31.0 34.0 3 32.48127634671717 34.0 31.0 34.0 31.0 34.0 4 36.10552352595041 37.0 35.0 37.0 35.0 37.0 5 36.02228478088213 37.0 35.0 37.0 35.0 37.0 6 36.05479795625038 37.0 35.0 37.0 35.0 37.0 7 36.004582037768856 37.0 35.0 37.0 35.0 37.0 8 35.97070389746525 37.0 35.0 37.0 35.0 37.0 9 37.66787139425782 39.0 37.0 39.0 35.0 39.0 10-11 37.589385910475016 39.0 37.0 39.0 35.0 39.0 12-13 37.519330572320634 39.0 37.0 39.0 35.0 39.0 14-15 38.76810548011717 40.0 38.0 41.0 35.0 41.0 16-17 38.692466486814986 40.0 38.0 41.0 34.0 41.0 18-19 38.827448496287744 40.0 38.0 41.0 35.0 41.0 20-21 38.85083055463558 40.0 38.0 41.0 35.0 41.0 22-23 38.7756586076393 40.0 38.0 41.0 35.0 41.0 24-25 38.68037392643659 40.0 38.0 41.0 34.0 41.0 26-27 38.509818422685754 40.0 38.0 41.0 34.0 41.0 28-29 38.427523416624595 40.0 38.0 41.0 34.0 41.0 30-31 38.43135946649003 40.0 38.0 41.0 34.0 41.0 32-33 38.27683546786625 40.0 38.0 41.0 34.0 41.0 34-35 38.132006900388106 40.0 38.0 41.0 34.0 41.0 36-37 38.00393572928356 40.0 37.5 41.0 33.5 41.0 38-39 37.843805567256275 40.0 37.0 41.0 33.0 41.0 40-41 37.69318514326505 40.0 37.0 41.0 33.0 41.0 42-43 37.474958761660076 40.0 37.0 41.0 33.0 41.0 44-45 37.23038807448303 40.0 37.0 41.0 32.0 41.0 46-47 36.90353041990759 40.0 37.0 41.0 31.0 41.0 48-49 36.5714637118686 40.0 36.0 41.0 30.5 41.0 50-51 36.287977858307315 39.0 36.0 41.0 30.0 41.0 52-53 36.07228124387052 39.0 36.0 40.0 29.5 41.0 54-55 35.80378267314475 39.0 35.0 40.0 28.5 41.0 56-57 35.5447922327225 39.0 35.0 40.0 28.0 41.0 58-59 35.225379987588305 38.0 35.0 40.0 27.5 41.0 60-61 34.929467165278126 38.0 34.0 40.0 26.5 41.0 62-63 34.60940298459471 38.0 34.0 40.0 26.0 41.0 64-65 34.277122448126505 37.5 34.0 40.0 26.0 41.0 66-67 33.91442200407077 37.0 33.0 39.5 25.0 41.0 68-69 33.63728347861877 36.5 33.0 39.0 24.5 40.0 70-71 33.19751235542465 36.0 32.5 39.0 23.5 40.0 72-73 32.68557654896993 35.5 32.0 38.5 22.0 40.0 74-75 32.1835355324971 35.0 31.5 38.0 21.0 39.5 76-77 30.70212011691431 34.0 29.5 36.0 20.0 38.5 78-79 31.432627967472357 34.5 31.0 36.0 20.0 39.0 80-81 31.106076585947775 35.0 31.0 36.0 18.0 38.5 82-83 30.810730489361635 34.0 31.0 36.0 17.5 37.5 84-85 30.44949227806056 34.0 31.0 35.0 16.0 37.0 86-87 30.112905429955916 34.0 30.0 35.0 11.5 36.5 88-89 29.714703035720603 34.0 30.0 35.0 7.0 36.0 90-91 29.305097798371044 34.0 29.5 35.0 3.5 36.0 92-93 28.874479496586787 34.0 29.0 35.0 2.0 35.0 94-95 28.31278517156114 33.5 29.0 35.0 2.0 35.0 96-97 27.68873735116416 33.0 27.5 35.0 2.0 35.0 98-99 26.92574044122612 33.0 26.5 34.0 2.0 35.0 100-101 23.51224207950559 29.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14306439475368649 1101 2 0.13870126193570087 1101 3 0.1101540047906937 1101 4 0.1377087226635183 1101 5 0.09948020908658606 1101 6 0.16863606351945748 1101 7 0.18129318821736007 1101 8 0.21449368900398014 1101 9 0.27903642920799854 1101 10-11 0.23107905850667976 1101 12-13 0.32839238576415397 1101 14-15 0.4113233828614824 1101 16-17 0.32535953889093605 1101 18-19 0.17698417104514164 1101 20-21 0.25087500751634195 1101 22-23 0.24159432731077857 1101 24-25 0.27664704677535923 1101 26-27 0.3007275396045088 1101 28-29 0.26815444371598574 1101 30-31 0.20654807082359383 1101 32-33 0.10907214315749059 1101 34-35 0.3541297857086292 1101 36-37 0.27259717943078954 1101 38-39 0.2082427569833456 1101 40-41 0.2521312501335302 1101 42-43 0.1740243481768431 1101 44-45 0.08168288154537606 1101 46-47 -0.0761826815194766 1101 48-49 -0.05353653995332053 1101 50-51 -0.02961198174936186 1101 52-53 -0.1691350117790762 1101 54-55 -0.10525840858161928 1101 56-57 0.09549700633625235 1101 58-59 0.06539364067904785 1101 60-61 0.2807372001020454 1101 62-63 0.10234085577402396 1101 64-65 0.05924464546142616 1101 66-67 0.08385133142660806 1101 68-69 0.23780547218245118 1101 70-71 0.01392429782484328 1101 72-73 0.3345953811773441 1101 74-75 0.4236100526764979 1101 76-77 0.4246249073174404 1101 78-79 0.36978830504351734 1101 80-81 0.4976446771806451 1101 82-83 0.19104684463161803 1101 84-85 -0.03438524343393823 1101 86-87 -0.180709382552255 1101 88-89 0.05902693282914484 1101 90-91 0.06605290677765296 1101 92-93 0.2670587694428157 1101 94-95 0.08853559317211435 1101 96-97 0.2609500176691597 1101 98-99 0.007919307036182488 1101 100-101 0.5089320611309667 1102 1 0.24491691548892192 1102 2 0.26568973555068 1102 3 0.26585092184809866 1102 4 0.1859150854430922 1102 5 0.22502557459277028 1102 6 0.16959617560813456 1102 7 0.15364145606763202 1102 8 0.20974436813838082 1102 9 0.23957439437516825 1102 10-11 0.2886030445090384 1102 12-13 0.29633908988331115 1102 14-15 0.36287510207532137 1102 16-17 0.34333665075953945 1102 18-19 0.2569674719827404 1102 20-21 0.3505490062025842 1102 22-23 0.4202364952993065 1102 24-25 0.4661667051333751 1102 26-27 0.3391068233592094 1102 28-29 0.3682018193256056 1102 30-31 0.3179702127051556 1102 32-33 0.377280673119202 1102 34-35 0.4141233513616527 1102 36-37 0.37932585998646573 1102 38-39 0.38823731382819204 1102 40-41 0.42309462139535015 1102 42-43 0.6641846692557962 1102 44-45 0.5145523147012554 1102 46-47 0.4920108965654393 1102 48-49 0.6662035493883494 1102 50-51 0.7285934906375431 1102 52-53 0.5296430502896925 1102 54-55 0.5737168166625253 1102 56-57 0.6379754563138178 1102 58-59 0.5641689307706628 1102 60-61 0.5549962002078814 1102 62-63 0.563105619072374 1102 64-65 0.6470848216583747 1102 66-67 0.8682461080136434 1102 68-69 0.6786472796459648 1102 70-71 0.5515589277154902 1102 72-73 0.8382027314527747 1102 74-75 0.9080021412856887 1102 76-77 0.6702540033336888 1102 78-79 0.6359636317588233 1102 80-81 1.01754033324044 1102 82-83 0.8834081995740526 1102 84-85 0.8060752911712576 1102 86-87 0.5860577192868242 1102 88-89 0.7815158995200591 1102 90-91 0.7815010514849412 1102 92-93 0.727921811931342 1102 94-95 0.5068655861329745 1102 96-97 0.28897932670765414 1102 98-99 0.8127217154643347 1102 100-101 1.2101763428780892 1103 1 0.18028353328537605 1103 2 0.24112181103385666 1103 3 0.2405053324348856 1103 4 0.1601527812027399 1103 5 0.24563115958535775 1103 6 0.17649681092080982 1103 7 0.11260264987956958 1103 8 0.1528115920470654 1103 9 0.24438841383808096 1103 10-11 0.2225932866903051 1103 12-13 0.23665052084755445 1103 14-15 0.30385469622954986 1103 16-17 0.3132115146428305 1103 18-19 0.3388681579906532 1103 20-21 0.2768508091279074 1103 22-23 0.26853607574554417 1103 24-25 0.23414982564811027 1103 26-27 0.3142889713365662 1103 28-29 0.34900454794590985 1103 30-31 0.256201111247762 1103 32-33 0.48028426436961524 1103 34-35 0.5657431654926413 1103 36-37 0.5477917005235895 1103 38-39 0.4872096200355216 1103 40-41 0.6113853403146052 1103 42-43 0.41299941467446644 1103 44-45 0.4259710106981558 1103 46-47 0.3056273323660079 1103 48-49 0.3794500113679362 1103 50-51 0.5900464566129315 1103 52-53 0.6328245818130043 1103 54-55 0.3554165467610986 1103 56-57 0.5185826027196327 1103 58-59 0.566892278765053 1103 60-61 0.3995255760684344 1103 62-63 0.6725069489097635 1103 64-65 0.660743638674937 1103 66-67 0.5044332788748349 1103 68-69 0.41237816500781577 1103 70-71 0.39161039351749594 1103 72-73 0.5864988646230742 1103 74-75 0.7873236565459685 1103 76-77 0.5028355263401991 1103 78-79 0.6165078146834553 1103 80-81 0.6635273998722298 1103 82-83 0.7887850375297738 1103 84-85 0.5478560728106601 1103 86-87 0.6009093951698823 1103 88-89 0.4215224493443479 1103 90-91 0.5605164689196407 1103 92-93 0.679607732648055 1103 94-95 0.6030496544458153 1103 96-97 0.6454083949495235 1103 98-99 0.32655036151629346 1103 100-101 0.5983629337857934 1104 1 0.21839280794586813 1104 2 0.3230778064017841 1104 3 0.23504861769298202 1104 4 0.24165258819996183 1104 5 0.13568976224529194 1104 6 0.06767833880093832 1104 7 0.13371013487799388 1104 8 0.2845973940613149 1104 9 0.19041782044228484 1104 10-11 0.3311573460649768 1104 12-13 0.19297048435247888 1104 14-15 0.31400488747775057 1104 16-17 0.3821398228858044 1104 18-19 0.4346063058658771 1104 20-21 0.31571454917072117 1104 22-23 0.18777318842407453 1104 24-25 0.20383961755603508 1104 26-27 0.05801638123936925 1104 28-29 0.2276847598980396 1104 30-31 0.37409071366204927 1104 32-33 0.3402519802695281 1104 34-35 0.20656500638817477 1104 36-37 0.37765098395693286 1104 38-39 0.39398050819918495 1104 40-41 0.47654658535794425 1104 42-43 0.500289304838347 1104 44-45 0.49625911119996147 1104 46-47 0.5287911741739251 1104 48-49 0.5189822428308943 1104 50-51 0.773539455876012 1104 52-53 0.8569885288951511 1104 54-55 0.657720298033162 1104 56-57 0.5943361908166267 1104 58-59 0.6584457756243722 1104 60-61 1.0361539930947927 1104 62-63 0.6977727663642312 1104 64-65 0.5373095929859133 1104 66-67 0.35434730491266464 1104 68-69 0.5407513270950517 1104 70-71 0.5982900440070225 1104 72-73 0.5753047038342203 1104 74-75 0.6092522806116598 1104 76-77 0.7189196755488894 1104 78-79 0.585118545637588 1104 80-81 0.8911829425609312 1104 82-83 0.8519495811659468 1104 84-85 0.7909255187172448 1104 86-87 0.7207728895728032 1104 88-89 0.4455162030728097 1104 90-91 0.7719948701548596 1104 92-93 0.9006397711082421 1104 94-95 0.9544375198351567 1104 96-97 0.9089010432984743 1104 98-99 0.8545971734614106 1104 100-101 0.9682702924551272 1105 1 0.0953670853527413 1105 2 0.12531405442202015 1105 3 0.07601734331527865 1105 4 0.07574743456344635 1105 5 0.17732983505336364 1105 6 0.052377161241764725 1105 7 0.11477699605973868 1105 8 0.058708314934875716 1105 9 0.10268341457441466 1105 10-11 0.14256045492926006 1105 12-13 0.14296305495706463 1105 14-15 0.09234971948899329 1105 16-17 0.1869738265532277 1105 18-19 0.04069469540933568 1105 20-21 0.240621090753379 1105 22-23 0.12839036856327368 1105 24-25 9.706889761602611E-4 1105 26-27 0.08538272810433511 1105 28-29 0.02386182940679049 1105 30-31 0.08413323006582374 1105 32-33 -0.005768734235687134 1105 34-35 -0.0028721831390825514 1105 36-37 -0.10145637418382591 1105 38-39 -0.03183106882506337 1105 40-41 0.05965365983087878 1105 42-43 0.06346027211186112 1105 44-45 -0.06271762466139563 1105 46-47 0.09099294304542838 1105 48-49 0.07795222469641772 1105 50-51 -0.08168357795137382 1105 52-53 -0.08232090880092358 1105 54-55 -0.03946767580185906 1105 56-57 0.18781993563715815 1105 58-59 0.08434768734289122 1105 60-61 -0.01929377994393633 1105 62-63 0.22776219636075723 1105 64-65 -0.028708183531115594 1105 66-67 -0.21403437014584625 1105 68-69 -0.01727393960336343 1105 70-71 -0.3221858763093337 1105 72-73 -0.1740545108111462 1105 74-75 0.17255326230494816 1105 76-77 -0.04864493486374499 1105 78-79 0.22731717390103512 1105 80-81 -0.01571322488413074 1105 82-83 0.17647485968385368 1105 84-85 0.31792546130828114 1105 86-87 0.2672901916216297 1105 88-89 -0.03561650956830675 1105 90-91 -0.09606244425377852 1105 92-93 -0.021148957148113823 1105 94-95 -0.1933804329611668 1105 96-97 0.014765706729971129 1105 98-99 0.10319909632402613 1105 100-101 0.3784767621077343 1106 1 0.11512763881394505 1106 2 0.15590769956059347 1106 3 0.13486402296231859 1106 4 -0.004689479420839859 1106 5 0.05972623368904095 1106 6 0.05157624432998631 1106 7 0.10242492403245507 1106 8 0.16492646813095035 1106 9 0.15046225103546362 1106 10-11 0.22521715156126731 1106 12-13 0.2187498177337588 1106 14-15 0.28462156173597464 1106 16-17 0.2297193144911489 1106 18-19 -0.029841228475937953 1106 20-21 0.17226007055094072 1106 22-23 0.2507891147140384 1106 24-25 0.02855053901137694 1106 26-27 0.1054815999162031 1106 28-29 0.14832500794221914 1106 30-31 0.14994995818214818 1106 32-33 -0.039517715828409905 1106 34-35 0.04352230986670946 1106 36-37 0.1558416359519157 1106 38-39 0.02631135629610526 1106 40-41 0.18746204736419259 1106 42-43 -0.06924337482187326 1106 44-45 -0.21793670305459045 1106 46-47 -0.21063857993950563 1106 48-49 0.06369452277559873 1106 50-51 -0.13645662847818585 1106 52-53 -0.12038862770670988 1106 54-55 -0.18804121516416927 1106 56-57 0.07134176062299957 1106 58-59 0.12340274347612024 1106 60-61 0.08750089533949534 1106 62-63 0.2181580978755946 1106 64-65 -0.32386677888855786 1106 66-67 -0.20972944174505415 1106 68-69 -0.4958839344940671 1106 70-71 -0.49042330674694057 1106 72-73 -0.5912321072318747 1106 74-75 -0.5343560269503307 1106 76-77 -0.43734855417965335 1106 78-79 -0.42373161298602113 1106 80-81 -0.25766953348570354 1106 82-83 -0.35356988119191257 1106 84-85 -0.4798533751527714 1106 86-87 -0.2956888050228095 1106 88-89 -0.5345739366917712 1106 90-91 -0.1223546132196276 1106 92-93 -0.4973596535315572 1106 94-95 -0.4017983458821668 1106 96-97 -0.13709780598112786 1106 98-99 -0.17568914814896175 1106 100-101 0.3770717052840631 1107 1 0.13332133635628907 1107 2 0.1206343752738519 1107 3 0.2846772276330398 1107 4 0.21695197406460665 1107 5 0.14055081360896793 1107 6 0.024738027513386385 1107 7 0.09729014659531998 1107 8 0.14163987551229695 1107 9 -0.10269149073464945 1107 10-11 0.04843589475538579 1107 12-13 0.1728676914500724 1107 14-15 0.33608241848154563 1107 16-17 0.2673212980397466 1107 18-19 0.35110397264315907 1107 20-21 0.1667232224498889 1107 22-23 0.10402913414745285 1107 24-25 0.3221835825187682 1107 26-27 0.5105007820374539 1107 28-29 0.23586333030237228 1107 30-31 0.16545061992344756 1107 32-33 0.20701861293137114 1107 34-35 0.31936372649568057 1107 36-37 0.46184672884699296 1107 38-39 0.4717972754463986 1107 40-41 0.26987815699546047 1107 42-43 0.40415294958214787 1107 44-45 0.29866546477641265 1107 46-47 0.3024245284017866 1107 48-49 0.5897788570973574 1107 50-51 0.5688590807577114 1107 52-53 0.5444729092084017 1107 54-55 0.8649776654040906 1107 56-57 0.705398821151384 1107 58-59 0.9057694319216125 1107 60-61 0.8480468737952123 1107 62-63 0.8956890489402483 1107 64-65 0.9761449481724682 1107 66-67 1.0258965320198357 1107 68-69 1.0261210578833513 1107 70-71 0.9929703371859802 1107 72-73 0.6459937727165226 1107 74-75 0.8748555794895019 1107 76-77 0.7662457900263391 1107 78-79 0.8754173374420127 1107 80-81 0.8297263888476465 1107 82-83 0.8385822020367151 1107 84-85 0.931929808241712 1107 86-87 0.6512504320481121 1107 88-89 0.7333977843382016 1107 90-91 1.1267532924948007 1107 92-93 0.8820149590566011 1107 94-95 1.108940291433342 1107 96-97 0.9854416053204069 1107 98-99 1.173178352814336 1107 100-101 1.579041013890052 1108 1 0.07415802461114396 1108 2 0.01839294990050888 1108 3 0.09187288174293684 1108 4 0.07993912863052799 1108 5 -0.049505106867876236 1108 6 0.04281928877962571 1108 7 0.20140787132899618 1108 8 0.17860696503726103 1108 9 0.12822844870786554 1108 10-11 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2316 86-87 0.04230747271354929 2316 88-89 0.08428129384003924 2316 90-91 0.1780303289523495 2316 92-93 -0.31815780164759744 2316 94-95 -0.2518125066677719 2316 96-97 0.08120081897768827 2316 98-99 -0.0958497719171163 2316 100-101 -1.1719376177147858 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 6.0 5 5.0 6 13.0 7 59.0 8 189.0 9 339.0 10 572.0 11 866.0 12 1376.0 13 1833.0 14 2335.0 15 3341.0 16 4422.0 17 5989.0 18 5400.0 19 3440.0 20 3026.0 21 3008.0 22 3131.0 23 3333.0 24 3894.0 25 4707.0 26 5632.0 27 6866.0 28 8546.0 29 10560.0 30 13429.0 31 16890.0 32 21379.0 33 28384.0 34 38868.0 35 55202.0 36 86629.0 37 144550.0 38 124603.0 39 9171.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.78819777791715 22.073847509604423 15.065072067334675 22.072882645143757 2 14.839700302270241 23.77146440285549 39.436298154222136 21.952537140652133 3 18.248948539531053 27.085235822968784 30.933356056026444 23.732459581473716 4 12.420597725690564 19.969420779400345 39.027895902030735 28.582085592878354 5 12.594534977941837 39.97816692176105 34.76861438732331 12.6586837129738 6 28.796097711553486 40.23929491281266 18.19020762258157 12.774399753052279 7 25.914719434592616 35.08651208854105 22.177705894269074 16.82106258259726 8 24.431746930034695 37.228333392283524 20.976890452319477 17.363029225362304 9 25.365196448840344 17.91608922272562 21.690562931475224 35.02815139695881 10-11 22.89363727507274 29.261909758150683 28.831933982477764 19.012518984298808 12-13 24.44774386891192 26.845596581317505 28.594568436351476 20.1120911134191 14-15 21.27456213403988 29.139670157589943 27.123010832901922 22.46275687546825 16-17 20.287816281185993 31.79709129026968 27.90525310533542 20.009839323208904 18-19 20.137413453399283 30.69246123138339 29.94269214289906 19.22743317231827 20-21 21.548451821495235 29.784449564220587 29.806636451770046 18.86046216251413 22-23 20.89561717373986 29.927514270854015 29.54141140173107 19.635457153675052 24-25 20.547893444171546 29.961932861949343 29.92744331131844 19.562730382560666 26-27 20.709051663497906 30.102217595253695 29.721644967702776 19.46708577354562 28-29 20.362882480803613 30.68793489305329 29.535026173970085 19.414156452173017 30-31 20.979041778976825 30.156654080425998 29.530745182085145 19.333558958512032 32-33 20.74995277122381 30.29707905092267 29.36858140834201 19.584386769511514 34-35 20.898835036993926 30.542336421251655 29.38902947616858 19.169799065585842 36-37 20.828851339292175 30.240154486094163 29.465553363465197 19.46544081114847 38-39 20.570285560842976 30.681208811793866 29.334082630063683 19.41442299729948 40-41 21.24408852561016 30.343036337430135 29.180396132089047 19.23247900487066 42-43 20.910651871239917 30.52207899111567 28.890551998893883 19.676717138750536 44-45 20.95872307449911 30.732933115110434 28.632510924542682 19.675832885847775 46-47 21.186314647982655 30.40071962166807 28.43269262460138 19.98027310574789 48-49 21.09444713304725 30.562071621163177 28.438807846718557 19.90467339907101 50-51 21.025390774168184 30.927977605502793 28.157366041819248 19.88926557850977 52-53 21.206313887272223 31.01452747132609 27.809432245327127 19.96972639607456 54-55 21.206362374973672 30.809654500465456 27.960155570454475 20.023827554106393 56-57 21.144264208642635 30.925633839955108 27.74948027455009 20.18062167685216 58-59 21.249990956424437 30.986666152187702 27.725271447944777 20.038071443443087 60-61 21.138944692714524 30.919392479225298 28.012662979996637 19.928999848063533 62-63 21.06214325910443 30.63188359192861 28.07267465230324 20.233298496663718 64-65 20.99581506544286 30.776712921206506 28.11076651988045 20.11670549347018 66-67 21.10550703573174 30.50124765261235 27.908107812620585 20.485137499035318 68-69 21.12307632944687 30.61272886293626 27.917258853868564 20.346935953748304 70-71 21.232006212363473 30.30770009911807 27.923078592668155 20.537215095850307 72-73 21.081634227982907 30.426820837500042 27.89014041936096 20.601404515156094 74-75 20.771743778878896 30.83389871928025 27.632581664774904 20.76177583706595 76-77 20.717402418672847 30.449489866461732 27.87687662581954 20.956231089045875 78-79 20.8284463194857 30.593845537938634 27.447559896233187 21.13014824634248 80-81 20.765772790780307 30.48186979177553 27.459069766694057 21.293287650750113 82-83 20.842823960359265 30.398521914403858 27.590689926039442 21.16796419919744 84-85 20.7470277432073 30.423705375949883 27.426927665705126 21.402339215137687 86-87 20.520896893864666 30.300033845354868 27.341821201662842 21.837248059117627 88-89 20.496664732012945 30.31042349623546 27.43456877854753 21.75834299320406 90-91 20.415895276964743 30.270015201367883 27.552820934960494 21.761268586706883 92-93 20.17080646730135 30.415577086788304 27.453152419530525 21.960464026379825 94-95 19.88861628890311 31.00938272716457 27.38048920086046 21.72151178307186 96-97 19.710240231138965 31.1642885796353 27.294929686388347 21.830541502837384 98-99 19.901490583966133 31.164153955731056 26.843695284828396 22.09066017547442 100-101 19.677535527041385 31.492015157775594 26.29772778062705 22.532721534555968 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 388.0 1 236.5 2 82.5 3 78.0 4 68.0 5 73.5 6 113.0 7 190.0 8 244.0 9 252.0 10 281.0 11 318.5 12 354.5 13 399.0 14 442.0 15 502.0 16 571.0 17 649.0 18 751.5 19 901.5 20 1122.5 21 1411.0 22 1851.0 23 2382.0 24 3068.5 25 3981.0 26 5312.5 27 7070.5 28 8408.0 29 9792.5 30 11381.0 31 12840.0 32 14835.5 33 17102.0 34 19393.5 35 21509.5 36 23857.5 37 25356.0 38 25254.0 39 24960.0 40 25115.0 41 24950.0 42 24401.0 43 24474.0 44 24546.5 45 24160.0 46 23802.5 47 23545.0 48 23452.0 49 22459.5 50 20113.5 51 17990.0 52 16066.5 53 13962.0 54 12102.5 55 10531.0 56 8868.5 57 7378.0 58 6278.5 59 5324.0 60 4287.5 61 3312.5 62 2416.5 63 1574.0 64 1016.5 65 668.5 66 488.5 67 356.5 68 248.5 69 163.0 70 117.5 71 96.0 72 57.0 73 26.5 74 20.5 75 16.0 76 9.5 77 4.0 78 1.5 79 1.0 80 0.5 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023312121981884072 2 0.005466290671614195 3 3.215465100949527E-4 4 4.8231976514242906E-4 5 3.215465100949527E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.01583616562217642 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.019331376186909E-4 20-21 8.038662752373818E-4 22-23 0.0015273459229510251 24-25 0.010691421460657177 26-27 0.005868223809232886 28-29 3.215465100949527E-4 30-31 0.0029743052183783124 32-33 0.0034566249835207416 34-35 0.0 36-37 0.009807168557896056 38-39 0.012459927266179416 40-41 7.234796477136435E-4 42-43 0.0 44-45 0.0 46-47 8.038662752373817E-5 48-49 0.004340877886281861 50-51 0.008922915655134936 52-53 0.0 54-55 0.004019331376186909 56-57 0.0049839709064717665 58-59 8.842529027611198E-4 60-61 0.003778171493615694 62-63 0.007074023222088959 64-65 1.6077325504747634E-4 66-67 0.004180104631234385 68-69 9.646395302848581E-4 70-71 0.0013665726679035488 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0036173982385682177 80-81 0.0033762383559970033 82-83 0.017443898172651184 84-85 0.012218767383608201 86-87 0.007636729614755126 88-89 8.038662752373818E-4 90-91 0.0016881191779985016 92-93 5.627063926661672E-4 94-95 0.0 96-97 0.004903584278948028 98-99 0.0036977848660919555 100-101 4.8231976514242906E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 621994.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.30486563043999 #Duplication Level Percentage of deduplicated Percentage of total 1 86.42162537787883 67.67233762779024 2 9.716288713468888 15.21665364269484 3 2.247485035432093 5.279670411178042 4 0.7537000654471552 2.3607352940197326 5 0.29814440960716415 1.1673078966377926 6 0.17260181230431884 0.8109337032036066 7 0.0804967670811217 0.4412301970980441 8 0.04990737987710893 0.3126392540195472 9 0.0464281168666991 0.3271992707449114 >10 0.18227202936404424 2.6999722911223016 >50 0.01932936980275639 1.0775412654148961 >100 0.010692771741146266 1.5699301018354466 >500 2.0563022573852414E-4 0.09065339971354855 >1k 8.225209029540966E-4 0.9731956445269758 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2041 0.3281382135518992 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1786 0.28714103351479275 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1150 0.1848892433045978 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1067 0.17154506313565726 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 1.6077325504747634E-4 0.0 0.0 0.0 4 0.0 1.6077325504747634E-4 0.0 0.0 0.0 5 0.0 1.6077325504747634E-4 0.0 0.0 0.0 6 0.0 1.6077325504747634E-4 0.0 0.0 0.0 7 0.0 1.6077325504747634E-4 0.0 0.0 0.0 8 0.0 1.6077325504747634E-4 0.0 0.0 0.0 9 0.0 1.6077325504747634E-4 0.0 0.0 0.0 10-11 0.0 1.6077325504747634E-4 0.0 0.0 0.0 12-13 0.0 1.6077325504747634E-4 0.0 0.0 0.0 14-15 0.0 1.6077325504747634E-4 0.0 0.0 0.0 16-17 0.0 1.6077325504747634E-4 0.0 0.0 0.0 18-19 0.0 1.6077325504747634E-4 0.0 0.0 0.0 20-21 0.0 1.6077325504747634E-4 0.0 0.0 0.0 22-23 0.0 1.6077325504747634E-4 0.0 2.4115988257121453E-4 0.0 24-25 0.0 3.215465100949527E-4 0.0 8.842529027611198E-4 0.0 26-27 0.0 3.215465100949527E-4 0.0 0.0017685058055222397 0.0 28-29 0.0 3.215465100949527E-4 0.0 0.005385904044090457 0.0 30-31 0.0 3.215465100949527E-4 0.0 0.009726781930372318 0.0 32-33 0.0 3.215465100949527E-4 0.0 0.016640031897413803 0.0 34-35 0.0 3.215465100949527E-4 0.0 0.021784776058933045 0.0 36-37 0.0 3.215465100949527E-4 0.0 0.026607973710357335 0.0 38-39 0.0 3.215465100949527E-4 0.0 0.036173982385682174 0.0 40-41 0.0 3.215465100949527E-4 0.0 0.05739605205194905 0.0 42-43 0.0 3.215465100949527E-4 0.0 0.09847361871657925 0.0 44-45 0.0 3.215465100949527E-4 0.0 0.14566056907301356 0.0 46-47 0.0 3.215465100949527E-4 0.0 0.24525960057492516 0.0 48-49 0.0 4.0193313761869085E-4 0.0 0.33915118152265133 0.0 50-51 0.0 5.627063926661672E-4 0.0 0.39791380624250394 0.0 52-53 0.0 6.430930201899054E-4 0.0 0.4767730878432911 0.0 54-55 0.0 6.430930201899054E-4 0.0 0.5534619305009374 0.0 56-57 0.0 6.430930201899054E-4 0.0 0.7223542349283112 0.0 58-59 0.0 6.430930201899054E-4 0.0 0.9205072717743258 0.0 60-61 0.0 6.430930201899054E-4 0.0 1.108933526689968 0.0 62-63 0.0 6.430930201899054E-4 0.0 1.3263793541416797 0.0 64-65 0.0 6.430930201899054E-4 0.0 1.5941472104232517 0.0 66-67 0.0 6.430930201899054E-4 0.0 1.9524304092965528 0.0 68-69 0.0 6.430930201899054E-4 0.0 2.3376431283903063 0.0 70-71 0.0 6.430930201899054E-4 0.0 2.6346717170905185 0.0 72-73 0.0 6.430930201899054E-4 0.0 2.9116036489097965 0.0 74-75 0.0 6.430930201899054E-4 0.0 3.205577545764107 0.0 76-77 0.0 6.430930201899054E-4 0.0 3.5837162416357713 0.0 78-79 0.0 6.430930201899054E-4 0.0 3.9826750740360835 0.0 80-81 0.0 6.430930201899054E-4 0.0 4.445219728807674 0.0 82-83 0.0 6.430930201899054E-4 0.0 4.90253925279022 0.0 84-85 0.0 6.430930201899054E-4 0.0 5.615649025553301 0.0 86-87 0.0 6.430930201899054E-4 0.0 6.368791338823204 0.0 88-89 0.0 6.430930201899054E-4 0.0 7.198702881378277 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTACG 75 3.0713243E-4 31.676344 1 GGTATCA 2685 0.0 29.198864 1 CATCCGA 70 0.008379452 27.14897 6 CCGATTG 35 0.008399076 27.138056 14-15 GTATAAT 405 0.0 24.637157 1 GTATCAA 3565 0.0 23.857218 1 GAGTACT 2280 0.0 20.933798 12-13 TACATGG 2145 0.0 20.813843 2 GTACATG 2155 0.0 20.505106 1 TAGGACG 165 6.325351E-5 20.147951 4 TCAACGC 4055 0.0 20.144403 4 CAACGCA 4060 0.0 20.127686 5 ATCAACG 4075 0.0 20.047146 3 TATCAAC 4190 0.0 19.723633 2 CGCAGAA 145 6.1323826E-4 19.659597 9 AACGCAG 4165 0.0 19.620264 6 GTACTTT 2415 0.0 19.566933 14-15 GGACGTG 170 7.932628E-5 19.563229 6 AGTACTT 2315 0.0 19.38642 12-13 TATAATG 475 0.0 18.998167 2 >>END_MODULE