FastQCFastQC Report
Thu 26 May 2016
SRR937072_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937072_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659642
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43710.6626321550174185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31920.48389884209919926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21130.32032526734198247No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7260.11005969904887804No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.040.7071231
GTATCAA32250.039.7944531
GTCTACG550.002570256134.568921
ATCAACG39800.032.2504043
TATCAAC39900.032.1695752
TCAACGC40150.032.0901074
CAACGCA40600.031.6173275
AACGCAG41750.030.5186796
CGCAGAG44400.028.4765388
ACGCAGA44500.028.4125467
GCAGAGT48050.025.8168149
GTATAGG1701.1139673E-725.164141
AGAGTAC39750.024.15109610-11
GAGTACT34000.023.90230412-13
CTTTGCG1452.3174525E-522.9500962
AGTACTT36050.021.75210212-13
CAGAGTA46750.021.09400610-11
GTACTTT39550.020.72679714-15
GTATAGC4700.020.2264961
CTAGTAC1656.277219E-520.1682663
Produced by FastQC (version 0.11.5)