FastQCFastQC Report
Thu 26 May 2016
SRR937072_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937072_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659642
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22610.3427616798202662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18580.2816679350314261No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12100.18343283174813005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10800.16372517213882684No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG200.002074116871.246254
TAACCGT600.003947828431.6698026
CGTAGTG700.00838807527.1434862
GTATCAA37900.023.188311
GTATAGC3350.022.6888141
GTACATG22200.022.6824231
TACATGG23500.021.426032
GAGTACT20950.021.31475812-13
ATCAACG42300.020.5485633
TCAACGC42350.020.5243034
CAACGCA42750.020.3322625
AGTACTT21950.020.23548712-13
GGTATCA28600.020.098141
TATCAAC43800.019.95482
AACGCAG43800.019.8478556
ACATGGG24750.019.7666363
TAGCCCT4850.019.5865974
AGAGTAC34000.019.4210410-11
GTGTATC2001.2822837E-519.0018791
GTACTTT23450.018.73781814-15
Produced by FastQC (version 0.11.5)