FastQCFastQC Report
Thu 26 May 2016
SRR937071_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937071_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120634
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81040.7231620671869674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56900.5077482924844329No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39090.3488203998807818No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14200.12671398511913792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33950.042.4087141
GTATCAA59850.041.7612841
ATCAACG73500.033.8793453
TATCAAC73750.033.7000662
TCAACGC73950.033.6746864
CAACGCA74900.033.1177145
AACGCAG76850.032.401056
ACGCAGA85450.028.8064257
CGCAGAG85150.028.7404948
GCAGAGT88850.026.8483729
CGATTCG950.001223606625.0102526
GAGTACT55950.023.22424512-13
GTACATG42600.022.5317041
ACATGGG42900.021.4899563
GTACTTT60000.021.37946714-15
AGAGTAC78650.021.32549710-11
TACATGG44150.021.3120652
TGGTATC20100.021.278332
AGTACTT59400.021.07553112-13
CAGAGTA87100.020.94769510-11
Produced by FastQC (version 0.11.5)