##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937071_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1120634 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.738606895739377 33.0 31.0 34.0 30.0 34.0 2 31.831113458988394 33.0 31.0 34.0 30.0 34.0 3 31.82883349960826 33.0 31.0 34.0 30.0 34.0 4 35.39841910918284 37.0 35.0 37.0 33.0 37.0 5 35.42539044862105 37.0 35.0 37.0 33.0 37.0 6 35.48199144412895 37.0 35.0 37.0 33.0 37.0 7 35.42268126792512 37.0 35.0 37.0 33.0 37.0 8 35.38961248721706 37.0 35.0 37.0 33.0 37.0 9 36.99975281849382 39.0 37.0 39.0 33.0 39.0 10-11 36.87850270471894 39.0 37.0 39.0 33.0 39.0 12-13 36.76922929341784 39.0 37.0 39.0 33.0 39.0 14-15 37.90660197709511 40.0 38.0 41.0 33.0 41.0 16-17 37.84415830681561 40.0 38.0 41.0 33.0 41.0 18-19 37.83569791742889 40.0 38.0 41.0 32.5 41.0 20-21 37.78867498219758 40.0 38.0 41.0 32.0 41.0 22-23 37.65345509773931 40.0 37.5 41.0 32.0 41.0 24-25 37.48601282845246 40.0 37.0 41.0 32.0 41.0 26-27 37.42795105270766 40.0 37.0 41.0 32.0 41.0 28-29 37.48231938349184 40.0 37.0 41.0 32.0 41.0 30-31 37.319385276548815 40.0 37.0 41.0 31.5 41.0 32-33 37.099307624077085 40.0 36.0 41.0 31.0 41.0 34-35 36.88880044688989 39.0 36.0 41.0 30.5 41.0 36-37 36.723152251314886 39.0 36.0 41.0 30.0 41.0 38-39 36.54695065471867 39.0 35.0 41.0 30.0 41.0 40-41 36.32437084721684 39.0 35.0 41.0 30.0 41.0 42-43 36.10649328862055 39.0 35.0 41.0 29.5 41.0 44-45 35.81838941170801 39.0 35.0 40.5 28.5 41.0 46-47 35.41602610665034 39.0 35.0 40.0 26.5 41.0 48-49 35.019542508972606 38.5 35.0 40.0 24.5 41.0 50-51 34.35646741041232 37.5 33.5 39.5 24.0 40.5 52-53 34.64597986496929 38.5 34.5 40.0 24.0 40.5 54-55 34.95257550636515 39.0 35.0 40.5 24.5 41.0 56-57 34.829723174560115 39.0 35.0 41.0 24.0 41.0 58-59 34.59865486858332 38.0 34.0 40.0 23.5 41.0 60-61 34.3104506020699 38.0 34.0 40.0 23.0 41.0 62-63 34.01755524105105 38.0 34.0 40.0 22.0 41.0 64-65 33.70906781339849 37.0 34.0 40.0 22.0 41.0 66-67 33.32323175987878 37.0 33.0 39.5 20.5 41.0 68-69 32.89663172811105 36.0 33.0 39.0 19.0 41.0 70-71 32.53899890597644 36.0 32.0 39.0 18.0 40.5 72-73 32.1580966667083 35.0 32.0 38.5 16.0 40.0 74-75 31.72878299248461 35.0 31.5 37.5 12.0 40.0 76-77 31.292006132242996 35.0 31.0 37.0 8.0 39.0 78-79 30.86320779130385 35.0 31.0 36.5 7.0 39.0 80-81 30.445717335008574 35.0 31.0 36.0 4.0 38.5 82-83 30.024432151799786 34.5 30.0 35.5 2.0 37.0 84-85 29.66436454721167 34.0 30.0 35.0 2.0 37.0 86-87 29.336741076926096 34.0 30.0 35.0 2.0 36.5 88-89 28.973421295445256 34.0 29.0 35.0 2.0 36.0 90-91 28.639886885459482 34.0 29.0 35.0 2.0 36.0 92-93 28.28842958539541 34.0 29.0 35.0 2.0 35.0 94-95 27.904309078610858 34.0 29.0 35.0 2.0 35.0 96-97 27.484830015866017 34.0 27.0 35.0 2.0 35.0 98-99 26.911701322644145 34.0 26.5 35.0 2.0 35.0 100-101 24.816773362221742 31.5 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3715472980867176 1101 2 0.34896609793245403 1101 3 0.38480897129590375 1101 4 0.11396874196659468 1101 5 0.15913075737461213 1101 6 0.05012222132427979 1101 7 0.13274393801605555 1101 8 0.12383184620158971 1101 9 0.24002176763962524 1101 10-11 0.23586525357738708 1101 12-13 0.2056602802495533 1101 14-15 0.5275897159739884 1101 16-17 0.5812585445778922 1101 18-19 0.5840992164989132 1101 20-21 0.5304996087175766 1101 22-23 0.5222832013652408 1101 24-25 0.4698493645077306 1101 26-27 0.618044501574218 1101 28-29 0.6045194590119252 1101 30-31 0.6036957877127449 1101 32-33 0.640480021730923 1101 34-35 0.5730373818767163 1101 36-37 0.6489131303387978 1101 38-39 0.7031270429191068 1101 40-41 0.6217321105113243 1101 42-43 0.7229929974332094 1101 44-45 0.5961739064635694 1101 46-47 0.7655962859833423 1101 48-49 0.7827763416242206 1101 50-51 0.6077939000517247 1101 52-53 0.7167904961305851 1101 54-55 0.7839148261463009 1101 56-57 0.9192510351755487 1101 58-59 0.7013443277340272 1101 60-61 0.7214348284315619 1101 62-63 0.7534285051014038 1101 64-65 0.8229856173864576 1101 66-67 0.8065923925775991 1101 68-69 0.7083894528832246 1101 70-71 0.8762910489361886 1101 72-73 0.8787526907989687 1101 74-75 0.8333593183812837 1101 76-77 0.7932189464105193 1101 78-79 0.8362069032624149 1101 80-81 0.8438303643221481 1101 82-83 0.6722730937784682 1101 84-85 0.7651145183618944 1101 86-87 0.6815250454486268 1101 88-89 0.664104018480522 1101 90-91 0.8049479252935114 1101 92-93 0.732220806104543 1101 94-95 0.7871866790938675 1101 96-97 1.0229607016164195 1101 98-99 1.0370279312144106 1101 100-101 1.0398791910818481 1102 1 0.41656141703798966 1102 2 0.3618158898949986 1102 3 0.4186873175587209 1102 4 0.2880897866976255 1102 5 0.284164954497534 1102 6 0.2890036958804316 1102 7 0.2902826491592023 1102 8 0.280702077933185 1102 9 0.42260228848262216 1102 10-11 0.4662414576134779 1102 12-13 0.3285712820884683 1102 14-15 0.4748274210675163 1102 16-17 0.47074533794638285 1102 18-19 0.4635433556473032 1102 20-21 0.4777616978465744 1102 22-23 0.46916109325237443 1102 24-25 0.5859703045658193 1102 26-27 0.5748538326903869 1102 28-29 0.6990635013378537 1102 30-31 0.5109948216291471 1102 32-33 0.7422125966612754 1102 34-35 0.5741281214147733 1102 36-37 0.6789863776024347 1102 38-39 0.6402667836632787 1102 40-41 0.6329898033771499 1102 42-43 0.593363301722178 1102 44-45 0.5604239367949262 1102 46-47 0.5583694526012479 1102 48-49 0.5858074556534874 1102 50-51 0.5650183545035432 1102 52-53 0.6462736497357326 1102 54-55 0.8126671723663037 1102 56-57 0.7219699146042231 1102 58-59 0.874730877737953 1102 60-61 0.8477952791912671 1102 62-63 0.8908902340603575 1102 64-65 0.9317390103357823 1102 66-67 0.852797166139112 1102 68-69 0.7611124954399031 1102 70-71 0.8855248668576152 1102 72-73 1.0039629287633645 1102 74-75 0.9145843247449221 1102 76-77 0.9583922395875106 1102 78-79 0.9910925123994048 1102 80-81 1.0397400315811716 1102 82-83 0.9134918673447459 1102 84-85 0.8766857542399684 1102 86-87 1.0769722486592315 1102 88-89 1.0334156987903498 1102 90-91 0.7974227740530182 1102 92-93 0.8928443951015126 1102 94-95 1.0367221929547021 1102 96-97 1.1295784098977144 1102 98-99 1.2859639546421135 1102 100-101 1.4930558956448756 1103 1 0.2554054233594094 1103 2 0.27825695122834304 1103 3 0.35311464670460424 1103 4 0.33071018929022955 1103 5 0.24493690484558073 1103 6 0.24511283788255867 1103 7 0.20953829913062805 1103 8 0.28973657654378115 1103 9 0.4262253893955048 1103 10-11 0.3806502822029856 1103 12-13 0.197907112508382 1103 14-15 0.30291034366052116 1103 16-17 0.3528823145934368 1103 18-19 0.4350951715354654 1103 20-21 0.22296249777288324 1103 22-23 0.2971467528919618 1103 24-25 0.543654212041254 1103 26-27 0.5535445257660783 1103 28-29 0.4848464301020172 1103 30-31 0.5152180461442484 1103 32-33 0.5259883310971958 1103 34-35 0.5190786313409674 1103 36-37 0.7006552220648814 1103 38-39 0.7439010195073905 1103 40-41 0.9200771736820315 1103 42-43 0.9250878834467642 1103 44-45 0.9501364677513777 1103 46-47 0.9081156298317907 1103 48-49 0.9657234830677695 1103 50-51 1.0258421784267355 1103 52-53 1.1276856931700152 1103 54-55 1.2014383768081132 1103 56-57 1.319042813970384 1103 58-59 1.2034387427557576 1103 60-61 1.0605390961741463 1103 62-63 1.2360377741349353 1103 64-65 1.214929601900245 1103 66-67 1.2563069501214486 1103 68-69 1.1716632228773562 1103 70-71 1.1196996253548193 1103 72-73 1.0622397601465252 1103 74-75 0.9329167145158124 1103 76-77 0.8703406468451576 1103 78-79 0.9223866328874131 1103 80-81 1.0396953129819018 1103 82-83 1.0112427006758438 1103 84-85 1.1588030994219416 1103 86-87 1.1175221801056168 1103 88-89 1.1461014263127716 1103 90-91 1.0292813327010144 1103 92-93 1.1306160941310317 1103 94-95 1.0971763729363353 1103 96-97 0.9938829507137221 1103 98-99 0.9937739685674885 1103 100-101 1.3650802082529232 1104 1 0.44842927972155877 1104 2 0.5026900398325935 1104 3 0.5102899257776485 1104 4 0.46834773130602514 1104 5 0.46173419400030724 1104 6 0.518370848963599 1104 7 0.4614440997768412 1104 8 0.5004915696259999 1104 9 0.573227125663351 1104 10-11 0.7110817878012412 1104 12-13 0.6835172941341696 1104 14-15 0.664572625918467 1104 16-17 0.7896956542531939 1104 18-19 0.7143939040809357 1104 20-21 0.6920274849975669 1104 22-23 0.6552960845233216 1104 24-25 0.6892288285192123 1104 26-27 0.7615394933186437 1104 28-29 0.8433106833493866 1104 30-31 0.7962323141929275 1104 32-33 0.8326254091284682 1104 34-35 0.8479706951062909 1104 36-37 0.8620399534052581 1104 38-39 0.8621393899769743 1104 40-41 0.9137582714567927 1104 42-43 1.1335470979482736 1104 44-45 1.102034710278808 1104 46-47 1.0565337260676344 1104 48-49 0.9105040771778974 1104 50-51 0.8907366062036601 1104 52-53 1.0715031520045102 1104 54-55 1.1690746696348313 1104 56-57 1.1365286792385163 1104 58-59 1.0348677625727234 1104 60-61 1.1489861442038887 1104 62-63 1.149828769056299 1104 64-65 1.2289614593709146 1104 66-67 1.0637497026203562 1104 68-69 1.1187899830267583 1104 70-71 1.0512763434097039 1104 72-73 1.0928758598213122 1104 74-75 1.0862313170112543 1104 76-77 1.0793042608016492 1104 78-79 1.2507333573876878 1104 80-81 1.282467599459796 1104 82-83 1.2702342376822813 1104 84-85 1.38456620698447 1104 86-87 1.33656643499058 1104 88-89 1.4162023481933126 1104 90-91 1.262142511053721 1104 92-93 1.3303874085508696 1104 94-95 1.2523469134887897 1104 96-97 0.9622585220340198 1104 98-99 1.250523788537297 1104 100-101 1.3900996809061787 1105 1 0.2912440988688445 1105 2 0.4498671897854649 1105 3 0.3381812184375832 1105 4 0.29297042523425887 1105 5 0.35834816866585584 1105 6 0.45070826823280896 1105 7 0.32597609177744147 1105 8 0.509625874615736 1105 9 0.47072348701335187 1105 10-11 0.3914092325578693 1105 12-13 0.43930370369206884 1105 14-15 0.5313620431270891 1105 16-17 0.6088842746705723 1105 18-19 0.6408155645650879 1105 20-21 0.7321698928727969 1105 22-23 0.5880834141478957 1105 24-25 0.6734875899609918 1105 26-27 0.5987644574945392 1105 28-29 0.6476632286047419 1105 30-31 0.49078564319212603 1105 32-33 0.5762541161086077 1105 34-35 0.39847257010769255 1105 36-37 0.45217611877430386 1105 38-39 0.3890372805722748 1105 40-41 0.49750177306409427 1105 42-43 0.4232263793514548 1105 44-45 0.28772508647135453 1105 46-47 0.45618150249817546 1105 48-49 0.6019071901076458 1105 50-51 0.497414850563473 1105 52-53 0.2982349132010924 1105 54-55 0.47704421295332367 1105 56-57 0.6648890596827144 1105 58-59 0.5253912445189499 1105 60-61 0.6297027051791133 1105 62-63 0.5786127401000698 1105 64-65 0.6099084306868789 1105 66-67 0.6223803329672535 1105 68-69 0.8078029471936503 1105 70-71 0.6941152889997895 1105 72-73 0.6718773062831005 1105 74-75 0.8332689385151681 1105 76-77 0.558531337306654 1105 78-79 0.6436445698286093 1105 80-81 0.7765487475410389 1105 82-83 0.7287884957463646 1105 84-85 0.785918862467863 1105 86-87 0.7655677689747797 1105 88-89 0.938434668403513 1105 90-91 0.8330340421661475 1105 92-93 0.8958608942088517 1105 94-95 0.935123838936395 1105 96-97 1.1633504544664817 1105 98-99 1.2354647265553993 1105 100-101 1.1542645460567975 1106 1 0.5568527809715249 1106 2 0.3795645833625443 1106 3 0.4660473201902917 1106 4 0.17120704361679628 1106 5 0.22465450840562085 1106 6 0.3334751413103447 1106 7 0.4860733737039169 1106 8 0.32376727914535763 1106 9 0.37413620487276944 1106 10-11 0.3801470336596324 1106 12-13 0.38871083743568846 1106 14-15 0.5081743234240079 1106 16-17 0.5464317043675777 1106 18-19 0.41609494877397424 1106 20-21 0.5312555157173904 1106 22-23 0.4814071366485777 1106 24-25 0.6200110774244862 1106 26-27 0.6060070763036975 1106 28-29 0.3908133296691574 1106 30-31 0.47061875409653453 1106 32-33 0.5585969865281015 1106 34-35 0.5531023032643603 1106 36-37 0.8105935667176141 1106 38-39 0.9121540291927062 1106 40-41 0.5604150606746074 1106 42-43 0.7930325053849998 1106 44-45 0.787582747819549 1106 46-47 0.6200458026162536 1106 48-49 0.7808344086676584 1106 50-51 0.6485685866160722 1106 52-53 0.7027855473019429 1106 54-55 0.7880034168718737 1106 56-57 0.5754964214853615 1106 58-59 0.8350404570571257 1106 60-61 0.9790180126504211 1106 62-63 0.9621769475196018 1106 64-65 0.8563065491205677 1106 66-67 1.0189786374324115 1106 68-69 0.803051056547794 1106 70-71 0.8574760512688684 1106 72-73 1.0725522848926587 1106 74-75 0.8512087629668059 1106 76-77 0.6847492446985086 1106 78-79 0.8750701687412814 1106 80-81 1.1027619763922551 1106 82-83 1.1608921658632525 1106 84-85 1.0640462091723073 1106 86-87 0.9638415460716594 1106 88-89 1.089212138772094 1106 90-91 1.1961248764292414 1106 92-93 1.1202314124246335 1106 94-95 1.1124599571908718 1106 96-97 0.8521700110835617 1106 98-99 1.0711539826436436 1106 100-101 1.2194385996633592 1107 1 0.17519554264459103 1107 2 0.2789920437683975 1107 3 0.20996286571500633 1107 4 0.3221103078741052 1107 5 0.19979788756166528 1107 6 0.17470971644883804 1107 7 0.216994419907671 1107 8 0.20698288036077628 1107 9 0.22397548343379725 1107 10-11 0.3420540985637288 1107 12-13 0.3126602859022327 1107 14-15 0.2883787181050579 1107 16-17 0.09935986334990332 1107 18-19 0.27713538804667337 1107 20-21 0.17806160456850506 1107 22-23 0.4767296054080461 1107 24-25 0.5282376702276821 1107 26-27 0.4448088466821005 1107 28-29 0.41475251932044443 1107 30-31 0.6307461588116823 1107 32-33 0.5442263404181347 1107 34-35 0.7901087809734193 1107 36-37 0.6635671575960629 1107 38-39 0.7413530599789198 1107 40-41 0.8634492274145558 1107 42-43 0.7838034797770419 1107 44-45 0.9893389592879274 1107 46-47 0.9908355359847576 1107 48-49 0.985770852864583 1107 50-51 0.7865638187095527 1107 52-53 0.8563087175689645 1107 54-55 0.7163646444124012 1107 56-57 0.661101838139821 1107 58-59 0.7698320077014742 1107 60-61 1.0000026635087451 1107 62-63 1.0605961129405514 1107 64-65 1.1434879152039485 1107 66-67 0.9671838332780709 1107 68-69 1.1020544859231762 1107 70-71 1.0060637543689879 1107 72-73 1.0496526551870389 1107 74-75 0.9350706695431938 1107 76-77 1.072092309250884 1107 78-79 1.0936322736547694 1107 80-81 1.108907495244047 1107 82-83 1.1787239408336987 1107 84-85 1.1067106677511198 1107 86-87 1.2157399154085482 1107 88-89 1.0635817101490233 1107 90-91 1.171774845652795 1107 92-93 1.3814579759169305 1107 94-95 1.4133637099631962 1107 96-97 1.1742308605134681 1107 98-99 1.2357478801141148 1107 100-101 1.4108153326703672 1108 1 0.10042056177417535 1108 2 0.15904678987740084 1108 3 0.169830181341311 1108 4 0.09266810288384875 1108 5 0.17691890486111816 1108 6 0.19154848363588428 1108 7 0.19531290973546334 1108 8 0.05404875712681445 1108 9 0.08427587706936635 1108 10-11 0.16696081718924205 1108 12-13 -0.08122021927655254 1108 14-15 0.19501530333623407 1108 16-17 0.1912469475279579 1108 18-19 0.3405118977061363 1108 20-21 0.39476035108771157 1108 22-23 0.3381229491015958 1108 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-2.030851659326512 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3588.0 3 1094.0 4 1055.0 5 1073.0 6 1126.0 7 1408.0 8 1702.0 9 2138.0 10 2627.0 11 3435.0 12 4330.0 13 5207.0 14 6051.0 15 7383.0 16 9514.0 17 12845.0 18 11223.0 19 7237.0 20 6565.0 21 6918.0 22 7402.0 23 8262.0 24 9143.0 25 10360.0 26 11768.0 27 14006.0 28 16355.0 29 19857.0 30 24026.0 31 29512.0 32 37630.0 33 49351.0 34 67577.0 35 97567.0 36 148116.0 37 220674.0 38 209849.0 39 42634.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25897672159484 19.073792330054783 15.355336747594661 22.31189420075571 2 14.92511318542045 20.019685197606695 41.48735779811597 23.567843818856883 3 18.352920795571546 25.388333605691482 30.79120488565869 25.46754071307828 4 12.265576210569765 17.059878297523333 38.635935198032314 32.038610293874584 5 13.048682447521214 36.931308619919605 35.12130415364002 14.89870477891916 6 29.941073433560007 37.10265656360844 18.210872608034272 14.745397394797276 7 26.77380799245348 33.34530343309608 22.070898722236514 17.809989852213935 8 25.50811238092686 34.18668524497917 20.71928437769352 19.58591799640045 9 25.909015381481833 16.767226670049435 21.447152844654003 35.87660510381473 10-11 23.851274064433255 26.684582906977734 28.2663603308177 21.19778269777131 12-13 25.30255206574959 24.50113518428212 28.662867237920693 21.5334455120476 14-15 22.564096609485972 25.402126423358055 27.469107821284688 24.564669145871285 16-17 20.892890560900565 28.592203588016364 28.17660754826028 22.338298302822793 18-19 20.81592919563635 26.795048695861855 31.40956769863491 20.979454409866886 20-21 21.656654679069476 25.881780414900334 31.497199545498177 20.964365360532007 22-23 21.374997097711567 25.686726093626934 31.545865980357313 21.39241082830419 24-25 21.176750004241136 25.940706242862614 31.475790257530694 21.406753495365553 26-27 20.9822747876644 26.04998200961927 31.570261141759737 21.397482060956587 28-29 20.914983947062588 25.967942344260166 31.754589854257816 21.362483854419438 30-31 21.087924905799685 25.82697854138944 31.57287405928024 21.512222493530633 32-33 20.93888020398561 25.960981323638777 31.576344168580967 21.523794303794645 34-35 21.22478750215377 26.047357045483153 31.327775640127165 21.400079812235912 36-37 21.332496481248278 25.78828743369279 31.361775888214176 21.517440196844753 38-39 21.03979180058567 26.086261695593173 31.312093779015786 21.561852724805373 40-41 21.150881610403875 26.084864069716307 31.306399516057386 21.457854803822432 42-43 21.022888590882065 26.03045978961353 31.045530760673334 21.90112085883107 44-45 21.115024789190333 26.159637297118994 30.927767865870997 21.797570047819683 46-47 21.423221909894213 26.460931872126224 30.43081032009505 21.68503589788451 48-49 21.473465261240914 26.591827670937544 30.057021958475516 21.87768510934602 50-51 21.470312062080378 26.81906790175248 29.91130481972662 21.79931521644052 52-53 21.519025195831194 26.91936887678815 29.614586355419668 21.94701957196099 54-55 21.419309280315456 26.91901668570429 29.584640635343195 22.07703339863706 56-57 21.435079147057156 27.087082073585496 29.46673886269824 22.011099916659116 58-59 21.533783192596072 26.79393136402352 29.642142253855617 22.03014318952479 60-61 21.45145220214054 26.76431935948119 29.99499148284248 21.789236955535795 62-63 21.28391031859372 26.81915642551125 30.119599314840702 21.777333941054337 64-65 21.23645115273441 26.81310401836805 29.944536986947046 22.0059078419505 66-67 21.211467796321124 26.93849088629035 29.610125461109643 22.23991585627888 68-69 21.17560457789138 26.887612391625947 29.912443675847868 22.024339354634805 70-71 21.337816404147585 26.722410504452576 29.731658400000715 22.20811469139913 72-73 21.329403285799394 26.81480952582831 29.55853903978615 22.297248148586142 74-75 21.29338623512137 27.20105506040712 29.287343291842028 22.218215412629483 76-77 21.34817768767132 26.972614987280796 29.40758724338965 22.271620081658234 78-79 21.288332575115977 26.95250642550146 29.3555569147414 22.403604084641167 80-81 21.33996432760754 27.089459504335856 29.150531068725595 22.42004509933101 82-83 21.494550198267955 27.195970530800956 29.19173105203898 22.11774821889211 84-85 21.355576539712516 27.120728105599422 28.948492824088028 22.575202530600038 86-87 21.195830758735475 27.10605081095368 29.232147768225474 22.465970662085372 88-89 21.431187023180293 26.856816992331588 29.09549165906018 22.616504325427943 90-91 21.242694242408955 26.87545511002334 29.345137136843967 22.536713510723743 92-93 21.283870820829513 26.89858991297297 29.271997531756096 22.545541734441425 94-95 21.157681956948945 26.751943602376755 29.352314127045574 22.73806031362873 96-97 21.030526288995183 26.814475883575234 29.423391326598736 22.731606500830846 98-99 20.976131425467436 27.09698774944251 29.124199496826574 22.80268132826348 100-101 20.97343211605928 27.051687617645452 29.103933590563884 22.87094667573138 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 447.0 1 340.5 2 283.5 3 351.0 4 306.5 5 282.0 6 528.0 7 1321.5 8 2440.5 9 2833.0 10 2290.5 11 1832.0 12 1789.0 13 1864.5 14 1977.0 15 2077.5 16 2147.0 17 2228.0 18 2377.0 19 2471.0 20 2676.5 21 3122.5 22 3654.0 23 4411.5 24 5333.0 25 6447.5 26 8286.5 27 10252.5 28 11717.0 29 13279.0 30 15435.0 31 17828.5 32 20247.0 33 22861.0 34 25496.0 35 27914.5 36 31749.0 37 34899.0 38 35147.5 39 34994.5 40 34655.5 41 34684.5 42 34725.5 43 35452.0 44 37292.5 45 38396.0 46 38420.0 47 39007.0 48 40882.5 49 40191.0 50 37396.5 51 35835.0 52 35158.5 53 34634.5 54 33733.0 55 31465.5 56 28620.5 57 26034.0 58 23955.0 59 21363.5 60 17781.5 61 14315.5 62 11126.0 63 8435.0 64 6157.0 65 4385.0 66 3107.0 67 2336.5 68 1717.5 69 1156.0 70 805.0 71 558.5 72 359.5 73 240.5 74 169.5 75 113.0 76 77.5 77 44.5 78 27.5 79 17.0 80 13.0 81 13.0 82 9.5 83 9.0 84 6.5 85 4.5 86 3.5 87 5.0 88 5.5 89 2.5 90 0.5 91 2.0 92 3.0 93 4.0 94 3.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.055861235693366436 2 0.09021678799679468 3 0.07004963261867836 4 0.12019981546160477 5 0.10119271769373409 6 0.09860489687087844 7 0.1051190665284116 8 0.09360772562674344 9 0.11029470817412286 10-11 0.10400362651856003 12-13 0.0885213191818203 14-15 0.09409851923107812 16-17 0.10328974491225502 18-19 0.0828995015321684 20-21 0.06482937337257302 22-23 0.07388674625256775 24-25 0.05809211571306957 26-27 0.052738003665782046 28-29 0.0665248421875474 30-31 0.0488562724314986 32-33 0.049525536437409534 34-35 0.04506377639800327 36-37 0.050284035644108604 38-39 0.04943630123662141 40-41 0.05947526132528551 42-43 0.05354112047287517 44-45 0.04359139558499921 46-47 0.03328472989397074 48-49 0.040289693155838566 50-51 0.024985856220675083 52-53 0.03649719712234325 54-55 0.04314521958105858 56-57 0.04778545002204109 58-59 0.05175641645711267 60-61 0.04836547882716391 62-63 0.05376420847484548 64-65 0.03819266593731763 66-67 0.05760132210873487 68-69 0.049525536437409534 70-71 0.024182739413581955 72-73 0.018427068962747872 74-75 0.028466029051411972 76-77 0.04260980837632983 78-79 0.06090302453789551 80-81 0.03926348834677513 82-83 0.06112611253986582 84-85 0.0661679013843949 86-87 0.06407087416587395 88-89 0.08709355596921028 90-91 0.06358008056153927 92-93 0.07223589503798743 94-95 0.07767924228606306 96-97 0.08856593678221435 98-99 0.11939669865451165 100-101 0.11377488100485975 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1120634.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.63313593513446 #Duplication Level Percentage of deduplicated Percentage of total 1 80.74584353315161 48.9587370714268 2 11.160732093752314 13.53420372352204 3 3.372293079872357 6.1341811417504175 4 1.4780997879760889 3.584873014801905 5 0.8334018182540152 2.526588286739196 6 0.4964126884883931 1.805943481262538 7 0.3414052359326699 1.449032905549056 8 0.24815617096783502 1.2037189477948167 9 0.1826783041946565 0.9968722605571005 >10 1.0360019357723624 11.488446381329295 >50 0.0717745083464539 2.9663052095031235 >100 0.031577692806721326 3.2006031242796578 >500 8.853548097320525E-4 0.3475004347463913 >1k 4.426774048660263E-4 0.5688485489489072 >5k 2.9511826991068416E-4 1.2341454677888057 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8104 0.7231620671869674 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5690 0.5077482924844329 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3909 0.3488203998807818 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1420 0.12671398511913792 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 8.92352007881253E-5 0.0 0.0 0.0 0.0 8 8.92352007881253E-5 0.0 0.0 0.0 0.0 9 8.92352007881253E-5 0.0 0.0 0.0 0.0 10-11 1.784704015762506E-4 0.0 0.0 0.0 0.0 12-13 1.784704015762506E-4 0.0 0.0 0.0 0.0 14-15 1.784704015762506E-4 0.0 0.0 0.0 0.0 16-17 1.784704015762506E-4 4.461760039406265E-5 0.0 0.0 0.0 18-19 1.784704015762506E-4 8.92352007881253E-5 0.0 0.0 8.92352007881253E-5 20-21 1.784704015762506E-4 8.92352007881253E-5 0.0 1.3385280118218795E-4 8.92352007881253E-5 22-23 1.784704015762506E-4 8.92352007881253E-5 0.0 4.461760039406264E-4 8.92352007881253E-5 24-25 3.569408031525012E-4 8.92352007881253E-5 0.0 7.138816063050024E-4 8.92352007881253E-5 26-27 3.569408031525012E-4 8.92352007881253E-5 0.0 0.0010708224094575034 8.92352007881253E-5 28-29 4.907936043346891E-4 8.92352007881253E-5 0.0 0.002275497620097195 8.92352007881253E-5 30-31 5.354112047287517E-4 1.3385280118218795E-4 0.0 0.004194054437041889 8.92352007881253E-5 32-33 5.800288051228143E-4 1.784704015762506E-4 0.0 0.00691572806107971 1.784704015762506E-4 34-35 6.246464055168771E-4 1.784704015762506E-4 0.0 0.009548166484329407 1.784704015762506E-4 36-37 7.138816063050024E-4 2.230880019703132E-4 0.0 0.011912899305214726 1.784704015762506E-4 38-39 8.031168070931277E-4 2.6770560236437585E-4 0.0 0.01566077773831599 1.784704015762506E-4 40-41 8.031168070931277E-4 2.6770560236437585E-4 0.0 0.01905171536826475 1.784704015762506E-4 42-43 8.477344074871903E-4 2.6770560236437585E-4 0.0 0.029402998659687284 1.784704015762506E-4 44-45 8.92352007881253E-4 2.6770560236437585E-4 0.0 0.04296674917948233 1.784704015762506E-4 46-47 9.369696082753155E-4 3.123232027584385E-4 0.0 0.054701178083120805 1.784704015762506E-4 48-49 9.815872086693781E-4 3.569408031525012E-4 0.0 0.06817569340212773 1.784704015762506E-4 50-51 9.815872086693781E-4 3.569408031525012E-4 0.0 0.08513038155187153 1.784704015762506E-4 52-53 9.815872086693781E-4 3.569408031525012E-4 0.0 0.11417643940840631 4.0155840354656383E-4 54-55 9.815872086693781E-4 3.569408031525012E-4 0.0 0.13697603320977234 4.461760039406265E-4 56-57 9.815872086693781E-4 3.569408031525012E-4 0.0 0.17802422557230996 4.461760039406265E-4 58-59 0.0010708224094575034 3.569408031525012E-4 0.0 0.2378118101003539 4.907936043346891E-4 60-61 0.0010708224094575034 3.569408031525012E-4 0.0 0.2926468409846569 5.354112047287517E-4 62-63 0.0010708224094575034 3.569408031525012E-4 0.0 0.3763494593239184 5.354112047287517E-4 64-65 0.0010708224094575034 3.569408031525012E-4 0.0 0.43047060860191644 5.354112047287517E-4 66-67 0.001160057610245629 4.461760039406265E-4 0.0 0.5314848558940742 5.354112047287517E-4 68-69 0.001160057610245629 4.461760039406265E-4 0.0 0.6528447289659246 6.246464055168771E-4 70-71 0.0012939104114278168 4.461760039406265E-4 0.0 0.7423030177560203 6.246464055168771E-4 72-73 0.0014277632126100048 4.461760039406265E-4 0.0 0.8969030031214473 6.246464055168771E-4 74-75 0.00151699841339813 4.461760039406265E-4 0.0 1.0403932059887526 6.246464055168771E-4 76-77 0.00151699841339813 4.461760039406265E-4 0.0 1.1357410180308647 8.031168070931277E-4 78-79 0.00151699841339813 4.461760039406265E-4 0.0 1.239878497350607 8.031168070931277E-4 80-81 0.0016954688149743806 4.461760039406265E-4 0.0 1.4348127934722665 8.031168070931277E-4 82-83 0.0016954688149743806 4.461760039406265E-4 0.0 1.5816493163691268 8.92352007881253E-4 84-85 0.001784704015762506 4.461760039406265E-4 0.0 1.831106320172331 8.92352007881253E-4 86-87 0.0018739392165506312 4.461760039406265E-4 0.0 2.1826037760767565 8.92352007881253E-4 88-89 0.0018739392165506312 4.461760039406265E-4 0.0 2.6434143529466354 9.369696082753155E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3395 0.0 42.408714 1 GTATCAA 5985 0.0 41.761284 1 ATCAACG 7350 0.0 33.879345 3 TATCAAC 7375 0.0 33.700066 2 TCAACGC 7395 0.0 33.674686 4 CAACGCA 7490 0.0 33.117714 5 AACGCAG 7685 0.0 32.40105 6 ACGCAGA 8545 0.0 28.806425 7 CGCAGAG 8515 0.0 28.740494 8 GCAGAGT 8885 0.0 26.848372 9 CGATTCG 95 0.0012236066 25.010252 6 GAGTACT 5595 0.0 23.224245 12-13 GTACATG 4260 0.0 22.531704 1 ACATGGG 4290 0.0 21.489956 3 GTACTTT 6000 0.0 21.379467 14-15 AGAGTAC 7865 0.0 21.325497 10-11 TACATGG 4415 0.0 21.312065 2 TGGTATC 2010 0.0 21.27833 2 AGTACTT 5940 0.0 21.075531 12-13 CAGAGTA 8710 0.0 20.947695 10-11 >>END_MODULE