FastQCFastQC Report
Thu 26 May 2016
SRR937071_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937071_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120634
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60990.5442454896067762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49880.44510518153116896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33300.2971532186244572No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17000.151699841339813No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12030.10734994654811472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49550.031.0643371
GTATCAA71850.029.5557161
TGTACCG1151.2823222E-424.7809095
ATCAACG88150.024.0862063
TCAACGC88250.024.004024
TATCAAC88750.023.977962
CAACGCA89750.023.655765
AACGCAG90600.023.3803566
GTACATG46850.021.4974521
GTATAGG4650.021.454931
GAGTACT54300.021.42943212-13
TACATGG48050.021.3531992
ACGCAGA100550.021.01957
CGCAGAG101000.020.8788248
GTACTTT56700.020.56470914-15
AGAGTAC81350.020.5508410-11
AGTACTT56200.020.45142212-13
ACATGGG46150.020.2757873
GTGTAGG5000.019.9530831
GCAGAGT106850.019.4245689
Produced by FastQC (version 0.11.5)