##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937071_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1120634 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.145965587337166 33.0 31.0 34.0 31.0 34.0 2 32.13999396770043 33.0 31.0 34.0 30.0 34.0 3 32.319594086918656 33.0 31.0 34.0 30.0 34.0 4 36.05570061233195 37.0 35.0 37.0 35.0 37.0 5 35.985875852419255 37.0 35.0 37.0 35.0 37.0 6 36.033860296939054 37.0 35.0 37.0 35.0 37.0 7 35.97194712992824 37.0 35.0 37.0 35.0 37.0 8 35.95454983518258 37.0 35.0 37.0 35.0 37.0 9 37.63673509816764 39.0 37.0 39.0 35.0 39.0 10-11 37.566543135403705 39.0 37.0 39.0 35.0 39.0 12-13 37.47791294927693 39.0 37.0 39.0 35.0 39.0 14-15 38.6852460303721 40.0 38.0 41.0 34.5 41.0 16-17 38.60722590961902 40.0 38.0 41.0 34.0 41.0 18-19 38.67119505565599 40.0 38.0 41.0 34.5 41.0 20-21 38.750559504708946 40.0 38.0 41.0 34.0 41.0 22-23 38.670090770046244 40.0 38.0 41.0 34.0 41.0 24-25 38.57535377295353 40.0 38.0 41.0 34.0 41.0 26-27 38.41108604593471 40.0 38.0 41.0 34.0 41.0 28-29 38.244281362157494 40.0 38.0 41.0 34.0 41.0 30-31 38.09749124156504 40.0 37.0 41.0 33.5 41.0 32-33 38.072894896995805 40.0 37.0 41.0 33.5 41.0 34-35 37.98615069683768 40.0 37.0 41.0 33.0 41.0 36-37 37.81752070702834 40.0 37.0 41.0 33.0 41.0 38-39 37.662771252701596 40.0 37.0 41.0 33.0 41.0 40-41 37.485728168161955 40.0 37.0 41.0 33.0 41.0 42-43 37.25282161704892 40.0 36.5 41.0 32.0 41.0 44-45 36.883808629757795 40.0 36.0 41.0 31.0 41.0 46-47 36.59617502235342 39.0 36.0 41.0 30.5 41.0 48-49 36.236426433608116 39.0 36.0 41.0 30.0 41.0 50-51 35.96781777101177 39.0 35.0 40.5 29.0 41.0 52-53 35.71068609376478 39.0 35.0 40.0 28.0 41.0 54-55 35.472790848751686 39.0 35.0 40.0 28.0 41.0 56-57 35.197241472238034 38.0 34.5 40.0 27.5 41.0 58-59 34.87649982063725 38.0 34.0 40.0 26.5 41.0 60-61 34.5390515547449 38.0 34.0 40.0 26.0 41.0 62-63 34.17426563891512 37.0 34.0 40.0 26.0 41.0 64-65 33.76509770362134 36.5 33.0 39.5 25.0 41.0 66-67 33.38277930171671 36.0 33.0 39.0 23.5 40.0 68-69 32.944722362519784 35.5 32.0 39.0 22.5 40.0 70-71 32.47385497852109 35.0 32.0 38.0 22.0 40.0 72-73 32.13438330445088 35.0 31.5 37.5 21.5 39.5 74-75 31.692065384416324 35.0 31.0 37.0 20.0 39.0 76-77 30.3054913557861 33.5 29.5 35.5 18.5 38.0 78-79 31.009301877330152 34.0 31.0 36.0 18.5 38.5 80-81 30.84295853954101 34.0 31.0 36.0 18.0 37.5 82-83 30.513506193815285 34.0 31.0 35.0 16.0 37.0 84-85 30.129490092215654 34.0 30.0 35.0 11.0 36.5 86-87 29.7812613217161 34.0 30.0 35.0 7.0 36.0 88-89 29.420284856607957 34.0 30.0 35.0 4.0 36.0 90-91 29.005067220876754 34.0 29.0 35.0 2.0 35.0 92-93 28.552442635151174 34.0 29.0 35.0 2.0 35.0 94-95 28.087935490088647 33.5 29.0 35.0 2.0 35.0 96-97 27.45839988792059 33.0 27.0 35.0 2.0 35.0 98-99 26.766579007954427 33.0 26.0 35.0 2.0 35.0 100-101 23.782798398049678 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2589505859914496 1101 2 0.16384828218524916 1101 3 0.12854458058823326 1101 4 0.19329129575100268 1101 5 0.21151191398092095 1101 6 0.22621146957256144 1101 7 0.2129009354519411 1101 8 0.24543768105425556 1101 9 0.27744230958001737 1101 10-11 0.2965813434016624 1101 12-13 0.28703633263533845 1101 14-15 0.30807405045977276 1101 16-17 0.3823323839688868 1101 18-19 0.3371626626793258 1101 20-21 0.34033434571976073 1101 22-23 0.3306031744236506 1101 24-25 0.3630696632977646 1101 26-27 0.38719089398470175 1101 28-29 0.4183823926518144 1101 30-31 0.2949760815861424 1101 32-33 0.4206421671698024 1101 34-35 0.5169200165325094 1101 36-37 0.4894162447532082 1101 38-39 0.4114801174669651 1101 40-41 0.3603258914119607 1101 42-43 0.5704182466928955 1101 44-45 0.49322509807539205 1101 46-47 0.29754921345909224 1101 48-49 0.22868412065997035 1101 50-51 0.3215728695849194 1101 52-53 0.2542678936016429 1101 54-55 0.11051869787439017 1101 56-57 0.053213788032628884 1101 58-59 0.29966553641531135 1101 60-61 0.18601774966953855 1101 62-63 0.2423997357824632 1101 64-65 0.2321633725251786 1101 66-67 0.11544693388334792 1101 68-69 0.10688567093703938 1101 70-71 0.16603409479512976 1101 72-73 0.43488302778278154 1101 74-75 0.27126402770282354 1101 76-77 0.07456867896621944 1101 78-79 0.2376841663294904 1101 80-81 0.45941495423121026 1101 82-83 0.4925324964202602 1101 84-85 0.4444251882820751 1101 86-87 0.3478509996622563 1101 88-89 0.38378562338539624 1101 90-91 0.47798660561434403 1101 92-93 0.5827571464509376 1101 94-95 0.6696975896401121 1101 96-97 0.4911752624258696 1101 98-99 0.5383979815825128 1101 100-101 0.6769353922884704 1102 1 0.20248429662312617 1102 2 0.05468749742372836 1102 3 0.13246981556707027 1102 4 0.1337097971676542 1102 5 0.10509403584400445 1102 6 0.15330796136406377 1102 7 0.28047563981037626 1102 8 0.231067752636271 1102 9 0.22515936912702017 1102 10-11 0.2876746283951306 1102 12-13 0.2722191653227881 1102 14-15 0.32210973917198515 1102 16-17 0.34054052609569396 1102 18-19 0.4395012701011112 1102 20-21 0.35970538034033694 1102 22-23 0.3370530491729724 1102 24-25 0.42018064277473854 1102 26-27 0.36931204338866763 1102 28-29 0.38600277247182646 1102 30-31 0.38573524582035645 1102 32-33 0.4785857722483371 1102 34-35 0.4465328218384812 1102 36-37 0.5235021526838466 1102 38-39 0.5179693635125773 1102 40-41 0.6688297345738476 1102 42-43 0.6222688165656436 1102 44-45 0.7307297667260784 1102 46-47 0.8120392778567407 1102 48-49 0.41106362189344736 1102 50-51 0.54022154202287 1102 52-53 0.4838691610957042 1102 54-55 0.3696407060721896 1102 56-57 0.5222954257833976 1102 58-59 0.6476429698825754 1102 60-61 0.6827580204393868 1102 62-63 0.6845905402653187 1102 64-65 0.6678002240585172 1102 66-67 0.5310121558256569 1102 68-69 0.3233195592748075 1102 70-71 0.4391329131804511 1102 72-73 0.4410819253864915 1102 74-75 0.37992107397539954 1102 76-77 0.4428550403209748 1102 78-79 0.5715759535484466 1102 80-81 0.5136688384296413 1102 82-83 0.6229091762424126 1102 84-85 0.6234248243057436 1102 86-87 0.5748984854897792 1102 88-89 0.675552502490536 1102 90-91 0.8072329367091058 1102 92-93 0.7422840485841249 1102 94-95 0.7349332107907038 1102 96-97 1.0673622891674235 1102 98-99 1.1048765938097418 1102 100-101 1.201622741375079 1103 1 0.11626147099794082 1103 2 -0.16047112391974494 1103 3 0.018931080763572083 1103 4 0.1222426067567568 1103 5 0.16819779283240166 1103 6 0.16474832061540212 1103 7 0.2201024004934311 1103 8 0.2550167984755163 1103 9 0.12317279007303483 1103 10-11 0.12464984445303884 1103 12-13 0.16120658419207246 1103 14-15 0.26151201248669764 1103 16-17 0.45250695494884496 1103 18-19 0.4430865837976157 1103 20-21 0.3093685598506326 1103 22-23 0.343270499622534 1103 24-25 0.4459984788774918 1103 26-27 0.38075821605790594 1103 28-29 0.3246730023164801 1103 30-31 0.3797150043935815 1103 32-33 0.4698325536271568 1103 34-35 0.4618925427228646 1103 36-37 0.6268394570287938 1103 38-39 0.5790442376520843 1103 40-41 0.25233881200231423 1103 42-43 0.1990043677626332 1103 44-45 0.4022262657069433 1103 46-47 0.37576104588705306 1103 48-49 0.4234587232392073 1103 50-51 0.16237442060382534 1103 52-53 0.29096072600520273 1103 54-55 0.19052907682156928 1103 56-57 0.1966288727019716 1103 58-59 0.31218706823486997 1103 60-61 0.27623666238918787 1103 62-63 0.12723088735319976 1103 64-65 0.1642544863223705 1103 66-67 0.29318768884899526 1103 68-69 0.30892553654165766 1103 70-71 0.03681577424065807 1103 72-73 0.17985979289959175 1103 74-75 0.3027267099498978 1103 76-77 0.41439634733830744 1103 78-79 0.3587040177786669 1103 80-81 0.40068998048900184 1103 82-83 0.49423341917888663 1103 84-85 0.5853386640861586 1103 86-87 0.14103013883477544 1103 88-89 0.4290128277020777 1103 90-91 0.7360802790380312 1103 92-93 0.5445913941506753 1103 94-95 0.6602023943990112 1103 96-97 0.902608460584549 1103 98-99 0.991134165786562 1103 100-101 0.7933155460630203 1104 1 0.26808150799072905 1104 2 0.19651037338557842 1104 3 0.16890147813166578 1104 4 0.1359737140841375 1104 5 0.1327470665181849 1104 6 0.16307031327142596 1104 7 0.23232293380268487 1104 8 0.11620774804816847 1104 9 0.21494987175740476 1104 10-11 0.2626101811252468 1104 12-13 0.28883985377127175 1104 14-15 0.4041337139452068 1104 16-17 0.37319866978102567 1104 18-19 0.4737297692087665 1104 20-21 0.3669792705447392 1104 22-23 0.4049486854220845 1104 24-25 0.5303043076567064 1104 26-27 0.25446467552014695 1104 28-29 0.44490981287433584 1104 30-31 0.31902357299085793 1104 32-33 0.4467188816201215 1104 34-35 0.4089235142140666 1104 36-37 0.33045438977203645 1104 38-39 0.24853846303938099 1104 40-41 0.4485420645127647 1104 42-43 0.5107468500587871 1104 44-45 0.6307047849035143 1104 46-47 0.7643740576084355 1104 48-49 0.6543967802568176 1104 50-51 0.7324843702373585 1104 52-53 0.6581965959971612 1104 54-55 0.5535657294987217 1104 56-57 0.41937371900972664 1104 58-59 0.45017915950335663 1104 60-61 0.4588392376082169 1104 62-63 0.4715383642222051 1104 64-65 0.3647755766229963 1104 66-67 0.5144269473975456 1104 68-69 0.32262016544851946 1104 70-71 0.37729852808322306 1104 72-73 0.48088759208037146 1104 74-75 0.3790408778535088 1104 76-77 0.4017643164507909 1104 78-79 0.5574933016259145 1104 80-81 0.6306226502474992 1104 82-83 0.5524191242568897 1104 84-85 0.599926358209089 1104 86-87 0.6661574721415136 1104 88-89 0.8216694329730281 1104 90-91 0.7960717971491746 1104 92-93 0.8975261486902717 1104 94-95 0.7518703032564957 1104 96-97 1.0011526829276072 1104 98-99 1.069490862804674 1104 100-101 1.0893809678795279 1105 1 0.2556634197948071 1105 2 0.07422586357415639 1105 3 0.18449705821233664 1105 4 0.2622359938249019 1105 5 0.21243406388395414 1105 6 0.1739407098449135 1105 7 0.28191261555859626 1105 8 0.3127578354432501 1105 9 0.24805371331697046 1105 10-11 0.4254029321695967 1105 12-13 0.3718103531492787 1105 14-15 0.5005989932383059 1105 16-17 0.4948268728615659 1105 18-19 0.3829510756134695 1105 20-21 0.38139777265818964 1105 22-23 0.39201629345118505 1105 24-25 0.41455109023527825 1105 26-27 0.35441282335169433 1105 28-29 0.3735711833462716 1105 30-31 0.5006116103244196 1105 32-33 0.5446660531461944 1105 34-35 0.5659841261371383 1105 36-37 0.5118455913251267 1105 38-39 0.5803223238551212 1105 40-41 0.4269590211841461 1105 42-43 0.45243903039185085 1105 44-45 0.4972020415751288 1105 46-47 0.6800037746373135 1105 48-49 0.906265876589373 1105 50-51 0.8187715525162886 1105 52-53 0.8968925983186935 1105 54-55 0.6440491694233188 1105 56-57 0.6729198561918963 1105 58-59 0.8328698146403255 1105 60-61 0.9004751791468664 1105 62-63 0.821959480314824 1105 64-65 0.8593856505657627 1105 66-67 0.7320390256909448 1105 68-69 0.7394162750736797 1105 70-71 0.5503430489624392 1105 72-73 0.609014750244107 1105 74-75 0.5142184124797389 1105 76-77 0.5579906258586433 1105 78-79 0.7059791845637413 1105 80-81 0.6782895276221801 1105 82-83 0.7374956540083382 1105 84-85 0.7452153468780978 1105 86-87 0.7621236321341378 1105 88-89 0.73439160433038 1105 90-91 0.885733307093318 1105 92-93 0.9846569128855087 1105 94-95 0.9791532046383971 1105 96-97 1.2961004145920718 1105 98-99 1.1315559963268171 1105 100-101 0.7966701342631239 1106 1 0.20692095601186367 1106 2 0.16477544936471133 1106 3 0.11830046593446752 1106 4 0.2057263595995522 1106 5 0.06898604286700305 1106 6 0.07778401203819385 1106 7 0.15662129710619155 1106 8 0.20133720054150217 1106 9 0.3490507182579847 1106 10-11 0.25667969005194635 1106 12-13 0.14625751220459193 1106 14-15 0.33724536772614755 1106 16-17 0.40125368105805137 1106 18-19 0.29568915584171407 1106 20-21 0.17864012978626675 1106 22-23 0.2421079214802475 1106 24-25 0.3652507699534908 1106 26-27 0.16140993772298629 1106 28-29 0.265491343653693 1106 30-31 0.25731163736480056 1106 32-33 0.24823926873889945 1106 34-35 0.2014814034643777 1106 36-37 0.16441954200379172 1106 38-39 0.2792820225888164 1106 40-41 0.341856366865656 1106 42-43 0.18424327390751927 1106 44-45 0.25206911154786127 1106 46-47 0.0699946316587372 1106 48-49 0.10296044797557613 1106 50-51 0.11829687888414497 1106 52-53 0.1336485616738301 1106 54-55 0.336037842597122 1106 56-57 0.36428723502760363 1106 58-59 0.2903161085621022 1106 60-61 0.2575572605740888 1106 62-63 0.27073738999045105 1106 64-65 0.28901683089812735 1106 66-67 0.2629324604296599 1106 68-69 0.3802395554919258 1106 70-71 0.28332763277453665 1106 72-73 0.1311993591141487 1106 74-75 0.22623441150279078 1106 76-77 0.13204320155386284 1106 78-79 0.09578329644588379 1106 80-81 0.1982815661733568 1106 82-83 0.30878115045304355 1106 84-85 0.17467564153100312 1106 86-87 0.059252990611057044 1106 88-89 0.27164071758833686 1106 90-91 0.5099089125480205 1106 92-93 0.5881882595043102 1106 94-95 0.698047054894964 1106 96-97 0.5492610066891466 1106 98-99 0.513911712908758 1106 100-101 0.6139647958896575 1107 1 0.14565433428857943 1107 2 -0.037706206896579886 1107 3 -0.004932331009385393 1107 4 0.05362525653265493 1107 5 0.020260578533481066 1107 6 0.08314986093073173 1107 7 0.1294667016454767 1107 8 0.12788212926715659 1107 9 0.20890064644628126 1107 10-11 0.17984458340912113 1107 12-13 0.2161708496414505 1107 14-15 0.04281688143876039 1107 16-17 0.17097161249007797 1107 18-19 0.11785308193916677 1107 20-21 0.1790416891261799 1107 22-23 0.2592538213737541 1107 24-25 0.2972206578881469 1107 26-27 0.3508961176332903 1107 28-29 0.35567854260631293 1107 30-31 0.40061605060942185 1107 32-33 0.2067829077849268 1107 34-35 0.21859603839710928 1107 36-37 0.2617502959778619 1107 38-39 0.2897958426785152 1107 40-41 0.39565603662950366 1107 42-43 0.3462578121142812 1107 44-45 0.2789828691076295 1107 46-47 0.272962848872055 1107 48-49 0.4912443924411889 1107 50-51 0.39261075954970437 1107 52-53 0.3456865486011651 1107 54-55 0.41725444038316795 1107 56-57 0.36247289027279095 1107 58-59 0.2078020149040114 1107 60-61 0.31883951477776407 1107 62-63 0.21559723503347783 1107 64-65 0.2510792435760649 1107 66-67 0.16695196194894635 1107 68-69 0.1858418456503017 1107 70-71 0.031242650703035224 1107 72-73 0.05828995910828638 1107 74-75 0.17623245744274385 1107 76-77 0.14205922484626043 1107 78-79 0.22982693060676596 1107 80-81 0.16561114771139884 1107 82-83 0.24853112846986747 1107 84-85 0.2622243709034535 1107 86-87 0.33737826540075133 1107 88-89 0.34190627434889365 1107 90-91 0.44234386677415927 1107 92-93 0.6127898755119681 1107 94-95 0.5883319568499985 1107 96-97 0.837011492761583 1107 98-99 0.880513895593829 1107 100-101 0.8325674475491418 1108 1 -0.02249747178082373 1108 2 -0.16395830720659887 1108 3 -0.06778780601470658 1108 4 0.0018917612223603442 1108 5 0.02593792358384661 1108 6 0.002983887136288388 1108 7 -0.030944919675526705 1108 8 -0.00524084437740413 1108 9 0.040138271380214974 1108 10-11 0.10975970327469753 1108 12-13 -0.03039489696453046 1108 14-15 -0.07047877121532053 1108 16-17 0.08586034536219955 1108 18-19 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-1.1880325081960272 2316 74-75 -1.291237774843026 2316 76-77 -1.6272380618997886 2316 78-79 -1.1837861867073087 2316 80-81 -1.3894210599794086 2316 82-83 -1.337955305260369 2316 84-85 -1.2776702221084513 2316 86-87 -1.1724987597798062 2316 88-89 -1.2119742643979343 2316 90-91 -1.2339571287362787 2316 92-93 -1.4131558594089775 2316 94-95 -1.2081251971613192 2316 96-97 -1.0830177869573312 2316 98-99 -1.1240888762195596 2316 100-101 -0.9406084259624734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 6.0 5 19.0 6 47.0 7 109.0 8 309.0 9 600.0 10 1016.0 11 1675.0 12 2510.0 13 3524.0 14 4461.0 15 6175.0 16 8472.0 17 11612.0 18 11034.0 19 6780.0 20 5777.0 21 5786.0 22 6127.0 23 6551.0 24 7714.0 25 9032.0 26 10821.0 27 13565.0 28 16798.0 29 20938.0 30 25754.0 31 32357.0 32 41668.0 33 54400.0 34 74284.0 35 107218.0 36 165343.0 37 250508.0 38 190956.0 39 16685.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.46758856804214 20.29429562367574 15.481981153798783 21.756134654483336 2 15.49846780425731 21.565231203608022 39.76685995356117 23.169441038573495 3 19.074067463858647 25.956630376583973 30.281902552204176 24.687399607353203 4 13.165404583476853 18.0173009207288 37.51679852654837 31.30049596924598 5 13.486576157084752 38.08559205723984 34.103592838951364 14.32423894672404 6 30.698082412792125 37.77349644440122 17.222858274876476 14.305562867930185 7 27.496934770852928 33.88082103523541 21.16462645252598 17.457617741385683 8 26.310998952378746 35.14091130556453 19.67145383773828 18.876635904318448 9 26.507941040518133 17.2996714359907 20.700960349230883 35.49142717426029 10-11 24.506126899820817 27.472488738497564 27.357375124037155 20.664009237644475 12-13 25.77225568307813 25.13515840873366 27.81030661784292 21.282279290345294 14-15 22.889676736561626 26.397155538739675 26.481928979488394 24.2312387452103 16-17 21.52924148294626 29.530828084816275 27.047546299683926 21.892384132553534 18-19 21.406810787464952 27.915447862549236 30.03094676763332 20.64679458235249 20-21 22.409255797143484 26.83195088323614 30.2599958057996 20.498797513820783 22-23 22.030877164176708 26.748072965838983 30.181977344967226 21.03907252501709 24-25 21.910787668940777 26.990143541096838 30.09651208966745 21.002556700294935 26-27 21.691539491549396 27.074456954106303 30.285503174414075 20.948500379930223 28-29 21.49962853974848 27.158630259843697 30.352608364883288 20.98913283552454 30-31 21.71215039349418 27.002634599285656 30.176737702079652 21.108477305140504 32-33 21.759938034247146 27.184618501024882 30.06561552142164 20.989827943306334 34-35 21.88382485158521 27.106240671787468 30.027106196052532 20.982828280574786 36-37 22.08930583287784 26.971521634856714 30.014479012125335 20.924693520140107 38-39 21.7482384562666 27.393477469373767 29.92612046317713 20.9321636111825 40-41 21.924123425038573 27.203652027560654 29.888192128561652 20.98403241883912 42-43 21.78014694826024 27.534170356287284 29.441588579674168 21.24409411577831 44-45 21.88819850280512 27.469171331457602 29.384390373620526 21.258239792116747 46-47 22.380659816058905 27.72211714744512 28.76826929126059 21.128953745235382 48-49 22.23833415504191 28.053041474345964 28.457768269258654 21.250856101353477 50-51 22.11573469256634 28.260660056111192 28.40575675154734 21.217848499775126 52-53 22.367415377902038 28.210638002631594 28.048851768382438 21.373094851083938 54-55 22.081898440365418 28.33293026933302 28.105057813655925 21.48011347664564 56-57 22.082379811650004 28.6497329841834 27.946197952663088 21.32168925150351 58-59 22.252790890675612 28.050570671330792 28.109422546648698 21.587215891344893 60-61 22.003498079179735 28.336627655349965 28.438179035618834 21.221695229851463 62-63 21.89153639814605 28.289298736251084 28.688001613372432 21.131163252230433 64-65 21.89749854545844 28.24974032417537 28.511423951570013 21.341337178796174 66-67 22.028918516033148 28.29034343582168 28.18477795778541 21.495960090359766 68-69 21.853799658853674 28.30024267523682 28.377074217395787 21.468883448513722 70-71 22.060081355986654 28.28612999556946 28.11644873131688 21.53733991712701 72-73 22.10770867205528 28.34422300233618 27.906301254468453 21.641767071140087 74-75 21.975922493666513 28.913710222187323 27.62599820993708 21.484369074209084 76-77 22.18874386558566 28.397290974809135 27.8213621065754 21.59260305302981 78-79 22.163252521791275 28.595248038606787 27.482973893303065 21.758525546298873 80-81 22.172306503336962 28.891920123545866 27.40613918721563 21.529634185901546 82-83 22.231592098071367 28.8098696025611 27.46600631351158 21.49253198585595 84-85 21.96164415834401 29.002561607302614 27.274581011444464 21.761213222908918 86-87 21.888632430085725 28.970390534751555 27.432649074673538 21.708327960489182 88-89 22.058343881861248 28.69697830966849 27.338794920108565 21.905882888361695 90-91 21.93417556603079 28.748184618954024 27.408271668008915 21.909368147006266 92-93 21.920423546334973 28.963362670919025 27.256782550576325 21.859431232169676 94-95 21.862162662603485 28.818903610237967 27.258668653639795 22.06026507351875 96-97 21.857987864704707 28.910585298882623 27.302897380948238 21.928529455464428 98-99 21.5891699424854 29.29099532833297 27.03672635275325 22.083108376428385 100-101 21.404710179361253 29.51467296320638 26.91784113284173 22.162775724590638 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 473.0 1 336.0 2 222.5 3 267.5 4 258.5 5 262.5 6 428.5 7 893.0 8 1569.0 9 1935.5 10 1846.5 11 1686.5 12 1749.5 13 1900.5 14 2015.5 15 2080.0 16 2143.0 17 2269.5 18 2339.0 19 2477.0 20 2734.0 21 3103.0 22 3663.0 23 4357.5 24 5134.5 25 6249.5 26 7810.0 27 9793.0 28 11590.0 29 13497.5 30 15623.0 31 18035.0 32 20513.5 33 22581.0 34 25412.5 35 28019.5 36 31846.0 37 34994.0 38 35458.0 39 35552.5 40 35009.5 41 34931.5 42 34654.0 43 35484.0 44 37592.5 45 38612.0 46 38499.0 47 38779.5 48 40972.5 49 40134.5 50 37091.0 51 35817.5 52 35092.0 53 34629.5 54 33959.0 55 32140.0 56 28736.0 57 25745.0 58 24103.5 59 21676.0 60 17834.5 61 14375.0 62 11639.0 63 8773.5 64 6164.0 65 4407.0 66 3226.5 67 2449.0 68 1746.0 69 1155.0 70 809.5 71 549.0 72 367.0 73 236.5 74 149.5 75 88.5 76 50.5 77 23.0 78 16.5 79 10.0 80 8.0 81 7.5 82 2.0 83 1.5 84 0.5 85 0.5 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017490099354472557 2 0.001784704015762506 3 0.00303399682679626 4 0.0 5 0.0014277632126100048 6 6.24646405516877E-4 7 0.0 8 0.0 9 0.0 10-11 1.784704015762506E-4 12-13 4.0155840354656383E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.003480172830736886 22-23 0.0 24-25 0.004372524838618139 26-27 0.0029893792264021972 28-29 0.005041788844529079 30-31 0.014277632126100047 32-33 0.0013385280118218793 34-35 0.0037032608327072 36-37 0.004149436836647827 38-39 0.002320115220491258 40-41 0.00455099524019439 42-43 0.003167849627978448 44-45 4.461760039406265E-4 46-47 3.123232027584385E-4 48-49 1.3385280118218795E-4 50-51 1.784704015762506E-4 52-53 0.001740086415368443 54-55 0.00455099524019439 56-57 6.692640059109396E-4 58-59 0.0021416448189150073 60-61 0.0016954688149743806 62-63 0.0 64-65 5.354112047287518E-4 66-67 2.2308800197031324E-4 68-69 4.461760039406265E-5 70-71 3.123232027584385E-4 72-73 0.0 74-75 2.677056023643759E-4 76-77 8.477344074871903E-4 78-79 1.784704015762506E-4 80-81 0.007004963261867836 82-83 0.0019185568169446938 84-85 0.0043279072382240765 86-87 0.002632438423249696 88-89 0.001740086415368443 90-91 1.3385280118218795E-4 92-93 8.92352007881253E-5 94-95 1.3385280118218795E-4 96-97 0.0016508512145803178 98-99 0.004372524838618139 100-101 0.0016508512145803178 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1120634.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.53277413449528 #Duplication Level Percentage of deduplicated Percentage of total 1 78.77433967472928 45.321062921002095 2 12.039794254908537 13.853655269868936 3 3.739185703313993 6.453771795470935 4 1.7234550370888855 3.9662059751917216 5 0.9321532675120247 2.6814681699250538 6 0.6002501473016968 2.0720433689343625 7 0.396234833958614 1.5957542444452126 8 0.27745949296767963 1.2770411472304875 9 0.20197347935352805 1.0458085111924207 >10 1.189155293869904 12.476776842572832 >50 0.08474462812950205 3.3274916161387904 >100 0.03938607410109755 4.001128283576602 >500 0.0010897324464036742 0.376530243268447 >1k 6.227042550878138E-4 1.0050061512167565 >5k 1.5567606377195345E-4 0.5462554599653325 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6099 0.5442454896067762 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4988 0.44510518153116896 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3330 0.2971532186244572 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1700 0.151699841339813 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1203 0.10734994654811472 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 8.92352007881253E-5 0.0 0.0 0.0 0.0 8 8.92352007881253E-5 0.0 0.0 0.0 0.0 9 8.92352007881253E-5 0.0 0.0 0.0 0.0 10-11 8.92352007881253E-5 0.0 0.0 0.0 0.0 12-13 1.784704015762506E-4 0.0 0.0 0.0 0.0 14-15 1.784704015762506E-4 0.0 0.0 0.0 0.0 16-17 1.784704015762506E-4 8.92352007881253E-5 0.0 0.0 0.0 18-19 2.230880019703132E-4 8.92352007881253E-5 0.0 0.0 0.0 20-21 2.6770560236437585E-4 8.92352007881253E-5 0.0 2.230880019703132E-4 0.0 22-23 2.6770560236437585E-4 8.92352007881253E-5 0.0 5.354112047287518E-4 0.0 24-25 2.6770560236437585E-4 8.92352007881253E-5 0.0 8.031168070931277E-4 0.0 26-27 2.6770560236437585E-4 8.92352007881253E-5 0.0 0.0012492928110337542 0.0 28-29 3.569408031525012E-4 1.784704015762506E-4 0.0 0.002409350421279383 0.0 30-31 3.569408031525012E-4 2.230880019703132E-4 0.0 0.004417142439012202 0.0 32-33 3.569408031525012E-4 2.6770560236437585E-4 0.0 0.007183433663444086 0.0 34-35 4.0155840354656383E-4 2.6770560236437585E-4 0.0 0.009905107287481906 0.0 36-37 5.354112047287517E-4 2.6770560236437585E-4 0.0 0.012671398511913791 0.0 38-39 5.354112047287517E-4 2.6770560236437585E-4 0.0 0.01637465934462099 0.0 40-41 5.354112047287517E-4 2.6770560236437585E-4 0.0 0.01989944977575194 0.0 42-43 6.692640059109396E-4 2.6770560236437585E-4 0.0 0.03011688026599229 0.0 44-45 8.92352007881253E-4 2.6770560236437585E-4 0.0 0.04385910118736358 0.0 46-47 8.92352007881253E-4 2.6770560236437585E-4 0.0 0.05581661809297237 0.0 48-49 8.92352007881253E-4 2.6770560236437585E-4 0.0 0.06924651581158522 0.0 50-51 8.92352007881253E-4 2.6770560236437585E-4 0.0 0.08593349835896466 0.0 52-53 8.92352007881253E-4 2.6770560236437585E-4 0.0 0.11520264421746976 1.3385280118218795E-4 54-55 8.92352007881253E-4 2.6770560236437585E-4 0.0 0.1380914732196239 1.784704015762506E-4 56-57 8.92352007881253E-4 2.6770560236437585E-4 0.0 0.17980892958807246 1.784704015762506E-4 58-59 8.92352007881253E-4 2.6770560236437585E-4 0.0 0.2395072789153283 1.784704015762506E-4 60-61 8.92352007881253E-4 2.6770560236437585E-4 0.0 0.29478848580357186 1.784704015762506E-4 62-63 8.92352007881253E-4 2.6770560236437585E-4 0.0 0.37902651534756215 1.784704015762506E-4 64-65 8.92352007881253E-4 2.6770560236437585E-4 0.0 0.4334599878283186 1.784704015762506E-4 66-67 8.92352007881253E-4 2.6770560236437585E-4 0.0 0.5347419407228409 1.784704015762506E-4 68-69 8.92352007881253E-4 2.6770560236437585E-4 0.0 0.6567264602002081 1.784704015762506E-4 70-71 8.92352007881253E-4 2.6770560236437585E-4 0.0 0.7469432481970028 1.784704015762506E-4 72-73 8.92352007881253E-4 2.6770560236437585E-4 0.0 0.9021678799679467 1.784704015762506E-4 74-75 8.92352007881253E-4 2.6770560236437585E-4 0.0 1.0459257884376165 2.230880019703132E-4 76-77 8.92352007881253E-4 2.6770560236437585E-4 0.0 1.1414074532809106 2.6770560236437585E-4 78-79 9.815872086693781E-4 2.6770560236437585E-4 0.0 1.2443848749904072 3.569408031525012E-4 80-81 0.0010708224094575034 2.6770560236437585E-4 0.0 1.4401222879191602 3.569408031525012E-4 82-83 0.0010708224094575034 2.6770560236437585E-4 0.0 1.5876280748219311 3.569408031525012E-4 84-85 0.0010708224094575034 2.6770560236437585E-4 0.0 1.83614810901686 3.569408031525012E-4 86-87 0.0010708224094575034 2.6770560236437585E-4 0.0 2.186663977712616 4.461760039406264E-4 88-89 0.0010708224094575034 2.6770560236437585E-4 0.0 2.648009965787224 5.354112047287517E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4955 0.0 31.064337 1 GTATCAA 7185 0.0 29.555716 1 TGTACCG 115 1.2823222E-4 24.780909 5 ATCAACG 8815 0.0 24.086206 3 TCAACGC 8825 0.0 24.00402 4 TATCAAC 8875 0.0 23.97796 2 CAACGCA 8975 0.0 23.65576 5 AACGCAG 9060 0.0 23.380356 6 GTACATG 4685 0.0 21.497452 1 GTATAGG 465 0.0 21.45493 1 GAGTACT 5430 0.0 21.429432 12-13 TACATGG 4805 0.0 21.353199 2 ACGCAGA 10055 0.0 21.0195 7 CGCAGAG 10100 0.0 20.878824 8 GTACTTT 5670 0.0 20.564709 14-15 AGAGTAC 8135 0.0 20.55084 10-11 AGTACTT 5620 0.0 20.451422 12-13 ACATGGG 4615 0.0 20.275787 3 GTGTAGG 500 0.0 19.953083 1 GCAGAGT 10685 0.0 19.424568 9 >>END_MODULE