FastQCFastQC Report
Thu 26 May 2016
SRR937070_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937070_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890325
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71020.7976862381714543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51240.5755201752169151No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34570.38828517676129504No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12550.14095976188470502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30850.042.2046281
GTATCAA51750.038.3822671
ATCAACG64650.030.6501243
TATCAAC64850.030.4090462
CAACGCA66000.030.0248785
TCAACGC66000.029.951194
AACGCAG67650.029.2909186
ACGCAGA75200.026.223767
CGCAGAG75200.025.7182438
TACATGG36750.024.8261032
GTACATG37100.024.5918961
GCAGAGT78700.024.0310679
ACATGGG36050.023.7264043
GAGTACT48900.023.65952312-13
GTACTTT51300.022.13586214-15
AGTACTT50600.021.6908912-13
AGAGTAC68850.021.29077710-11
CATGGGA18950.021.0637054
GTACTAG2308.9772584E-820.660311
CGCGGAA704.8503996E-420.35887182-83
Produced by FastQC (version 0.11.5)