FastQCFastQC Report
Thu 26 May 2016
SRR937070_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937070_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890325
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48400.5436217111728864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38700.43467273186757643No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25610.28764776907309125No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15570.17487995956532726No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9990.11220621683093253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40300.031.9457871
TATCACG600.00395125531.6653942
GTATCAA57550.028.7265361
ATCACGC700.00839451727.140243
CGATACA1000.001652119423.7530461
GCGCGCC1601.8621504E-623.7450439
ATCAACG70400.023.477853
GTACATG41000.023.4054431
TATCAAC70700.023.3795412
TCAACGC71250.023.1951584
GGGCGCG1852.545421E-723.1032877
CAACGCA72100.022.9217075
ATAGGAC1903.28826E-722.4978333
AACGCAG73700.022.4885226
GAGTACT42250.022.48114812-13
GTATAGG3600.022.4334321
ACATGGG39900.021.902653
TACATGG42950.021.8966772
AGTACTT42400.021.61755812-13
GTGTAGG4400.021.593681
Produced by FastQC (version 0.11.5)