FastQCFastQC Report
Thu 26 May 2016
SRR937069_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937069_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959172
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73060.7616986317365394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51650.5384852768846464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35260.3676087291955979No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12510.13042499155521636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29750.042.1719781
GTATCAA53000.041.9640121
ATCAACG64900.033.8319443
TCAACGC65900.033.318564
TATCAAC66350.033.3074722
CAACGCA66650.032.9436345
AACGCAG69100.031.9785866
AATAGCG650.00583552829.2390357
ACGCAGA75200.028.93777
CGCAGAG74900.028.9267358
GCAGAGT78150.027.1751699
GAGTACT48300.024.83556212-13
GTACTTT51100.023.28755614-15
GTACATG37650.022.7203251
ACATGGG35950.022.6061633
AGAGTAC67150.022.56976910-11
AGTACTT50750.022.4196712-13
TACATGG37400.022.3650932
CAGAGTA76550.021.31886110-11
ACGCGAA905.1555835E-621.11873278-79
Produced by FastQC (version 0.11.5)