FastQCFastQC Report
Thu 26 May 2016
SRR937069_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937069_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959172
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50990.5316043420783759No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40720.42453282622928945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26940.28086724800140117No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15580.16243176406317114No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9880.10300550891810853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64000.029.0936051
GGTATCA45400.028.8765091
CCGTCCA1351.3413939E-524.6320749
TATCAAC78600.023.8670162
ATCAACG78300.023.837153
ACTGCGT1201.7093768E-423.752368
TCAACGC78750.023.6406294
CAACGCA79050.023.5570535
AACGCAG80800.023.0468446
GTACATG40600.022.6969341
TATACCG1050.00219469122.6212945
GAGTACT47050.022.20683112-13
ACATGGG39700.021.8919333
TACATGG41700.021.8669762
TACGGTG1100.002877105321.5908031
GTACTTT48300.021.33713514-15
TAAGGTG4050.021.1132095
AGTACTT49400.020.66974312-13
ACGCAGA89700.020.6542267
CGCAGAG89900.020.5025928
Produced by FastQC (version 0.11.5)