##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937069_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 959172 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.245362666966926 33.0 31.0 34.0 31.0 34.0 2 32.37465855967439 34.0 31.0 34.0 31.0 34.0 3 32.47282969060815 34.0 31.0 34.0 31.0 34.0 4 36.10657733962209 37.0 35.0 37.0 35.0 37.0 5 36.026562493483965 37.0 35.0 37.0 35.0 37.0 6 36.06783559153103 37.0 35.0 37.0 35.0 37.0 7 36.01699590897149 37.0 35.0 37.0 35.0 37.0 8 35.98417906277498 37.0 35.0 37.0 35.0 37.0 9 37.667545549703284 39.0 37.0 39.0 35.0 39.0 10-11 37.583363567743845 39.0 37.0 39.0 35.0 39.0 12-13 37.501180184575865 39.0 37.0 39.0 35.0 39.0 14-15 38.716605572306115 40.0 38.0 41.0 35.0 41.0 16-17 38.63290994732957 40.0 38.0 41.0 34.0 41.0 18-19 38.79291826700529 40.0 38.0 41.0 35.0 41.0 20-21 38.81745401241905 40.0 38.0 41.0 35.0 41.0 22-23 38.73675003023441 40.0 38.0 41.0 34.5 41.0 24-25 38.62927191369222 40.0 38.0 41.0 34.0 41.0 26-27 38.444650177444714 40.0 38.0 41.0 34.0 41.0 28-29 38.36861063500602 40.0 38.0 41.0 34.0 41.0 30-31 38.35435511044943 40.0 38.0 41.0 34.0 41.0 32-33 38.18292496027824 40.0 38.0 41.0 34.0 41.0 34-35 38.03406114857398 40.0 37.0 41.0 33.0 41.0 36-37 37.88500654731372 40.0 37.0 41.0 33.0 41.0 38-39 37.72057305676145 40.0 37.0 41.0 33.0 41.0 40-41 37.550845416671876 40.0 37.0 41.0 33.0 41.0 42-43 37.301248889667335 40.0 37.0 41.0 32.0 41.0 44-45 37.02588326181331 40.0 36.0 41.0 31.5 41.0 46-47 36.64547599387805 39.5 36.0 41.0 30.5 41.0 48-49 36.25524619150684 39.0 36.0 41.0 30.0 41.0 50-51 35.942578599041674 39.0 35.0 40.0 29.0 41.0 52-53 35.696136355106276 39.0 35.0 40.0 28.0 41.0 54-55 35.40825420258306 38.5 35.0 40.0 28.0 41.0 56-57 35.124824327649264 38.0 34.5 40.0 27.0 41.0 58-59 34.764038149570666 38.0 34.0 40.0 26.0 41.0 60-61 34.428620727043736 38.0 34.0 40.0 26.0 41.0 62-63 34.06529902874563 37.0 33.5 40.0 25.5 41.0 64-65 33.66848125258035 36.5 33.0 39.0 24.5 40.5 66-67 33.261203412943665 36.0 33.0 39.0 23.0 40.0 68-69 32.95349791278311 36.0 32.0 39.0 22.5 40.0 70-71 32.49051160792851 35.0 32.0 38.0 22.0 40.0 72-73 31.972738466093674 35.0 31.0 37.5 20.0 39.5 74-75 31.464064317974252 35.0 31.0 37.0 18.5 39.0 76-77 30.062271417430868 33.5 29.5 35.5 18.0 37.5 78-79 30.78731395411876 34.0 30.5 36.0 18.0 38.0 80-81 30.519895284683038 34.0 30.5 35.5 13.0 37.5 82-83 30.21558437902691 34.0 30.0 35.0 11.5 37.0 84-85 29.867892307114886 34.0 30.0 35.0 7.5 36.5 86-87 29.542305238268007 34.0 30.0 35.0 6.5 36.0 88-89 29.146359047178194 34.0 29.0 35.0 2.0 36.0 90-91 28.717111738040728 34.0 29.0 35.0 2.0 35.0 92-93 28.235071499168033 33.5 29.0 35.0 2.0 35.0 94-95 27.64468207996063 33.0 27.0 35.0 2.0 35.0 96-97 26.967279591147364 33.0 26.0 34.5 2.0 35.0 98-99 26.160835595701293 32.5 25.0 34.0 2.0 35.0 100-101 22.616281021547753 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2446537085067746 1101 2 0.15075185477544295 1101 3 0.13908397818248375 1101 4 0.206802998802182 1101 5 0.2955826693681871 1101 6 0.33113131367246496 1101 7 0.314504942494807 1101 8 0.36102318999691363 1101 9 0.3756367860922012 1101 10-11 0.36607483133846586 1101 12-13 0.38482963860964503 1101 14-15 0.416973410992874 1101 16-17 0.4820608131106425 1101 18-19 0.39149995098198076 1101 20-21 0.3354950175393938 1101 22-23 0.33619838826023596 1101 24-25 0.36273026967977273 1101 26-27 0.29354182718841315 1101 28-29 0.45056471350635974 1101 30-31 0.3959372502692915 1101 32-33 0.3777884633184456 1101 34-35 0.46046737925369285 1101 36-37 0.3820124419741333 1101 38-39 0.3738558115897135 1101 40-41 0.487809402854225 1101 42-43 0.4432343021702181 1101 44-45 0.5016672950029886 1101 46-47 0.38520779978887987 1101 48-49 0.3828634995921689 1101 50-51 0.41830997940226666 1101 52-53 0.5477549172745384 1101 54-55 0.44969591973881506 1101 56-57 0.42468742741829857 1101 58-59 0.5472393528440094 1101 60-61 0.7012106290338451 1101 62-63 0.6755616597592322 1101 64-65 0.6942209841992124 1101 66-67 0.3918891560234812 1101 68-69 0.2517601569955019 1101 70-71 0.4467954548666597 1101 72-73 0.6167369617973186 1101 74-75 0.6133243990939867 1101 76-77 0.4632586287214693 1101 78-79 0.623223751075006 1101 80-81 0.8268720336187805 1101 82-83 0.8293682313757635 1101 84-85 0.7640202467078616 1101 86-87 0.39475178374907927 1101 88-89 0.3679179188823234 1101 90-91 0.7122021743577349 1101 92-93 0.8849533429701317 1101 94-95 0.649446931602494 1101 96-97 0.6580327531905894 1101 98-99 0.6614546645732382 1101 100-101 0.8639418673907926 1102 1 0.267883200282796 1102 2 0.2250050862099897 1102 3 0.21159527527262156 1102 4 0.18092427315936277 1102 5 0.1677059167738264 1102 6 0.1825249368403874 1102 7 0.07056903881590415 1102 8 0.2006698578914765 1102 9 0.21415866497932257 1102 10-11 0.3110878378152151 1102 12-13 0.36855173650009476 1102 14-15 0.3668693339481095 1102 16-17 0.3733385259892046 1102 18-19 0.3997198929006842 1102 20-21 0.3457293864288076 1102 22-23 0.26105678040236313 1102 24-25 0.36387726179010116 1102 26-27 0.19962673891846094 1102 28-29 0.3720928925239235 1102 30-31 0.5099100666067429 1102 32-33 0.46927533951849654 1102 34-35 0.2966602031099157 1102 36-37 0.2724330646197899 1102 38-39 0.352255977103745 1102 40-41 0.3321452146930284 1102 42-43 0.3137980339062736 1102 44-45 0.15178600591102054 1102 46-47 0.12904908797253967 1102 48-49 0.18910534689032943 1102 50-51 0.278562892928754 1102 52-53 0.38445084325746137 1102 54-55 0.49426536877385274 1102 56-57 0.4186759373125568 1102 58-59 0.4937134748752854 1102 60-61 0.30525335991458036 1102 62-63 0.31442289288929004 1102 64-65 0.26932928740276196 1102 66-67 0.2064137699291848 1102 68-69 0.2510885131332756 1102 70-71 0.1745727981140135 1102 72-73 0.5372062041921275 1102 74-75 0.4182471802344665 1102 76-77 0.26410209432306075 1102 78-79 0.2525293238015216 1102 80-81 0.5562790341561943 1102 82-83 0.5114800996911804 1102 84-85 0.6752050597854904 1102 86-87 0.4955475631410664 1102 88-89 0.5261831500847443 1102 90-91 0.43638286724592845 1102 92-93 0.2875326875244433 1102 94-95 0.4254499189290648 1102 96-97 0.4606441408592872 1102 98-99 0.4509987692709636 1102 100-101 0.7490514069034866 1103 1 0.17059320526431776 1103 2 0.23075550907658737 1103 3 0.18088545041397452 1103 4 0.14248042224481594 1103 5 0.17788961396475855 1103 6 0.17520857070687157 1103 7 0.023836721112921566 1103 8 -0.003995324637728004 1103 9 0.05760993189046815 1103 10-11 0.15123384992283917 1103 12-13 0.11760763932380058 1103 14-15 0.2043517790035949 1103 16-17 0.29987597325401083 1103 18-19 0.13197089413058194 1103 20-21 0.10827705566691748 1103 22-23 0.16849641813022487 1103 24-25 0.15383907011121778 1103 26-27 0.0938065184368071 1103 28-29 0.12629214782840847 1103 30-31 0.207968398274879 1103 32-33 0.053212600468697246 1103 34-35 0.2072390448635275 1103 36-37 0.08430428802000023 1103 38-39 -0.04350860229526177 1103 40-41 0.07970525330686229 1103 42-43 0.23240838590577795 1103 44-45 0.006907828079086187 1103 46-47 -0.25959388315432363 1103 48-49 -0.03663111645325756 1103 50-51 -0.03868837567104322 1103 52-53 -0.03237938811599861 1103 54-55 -0.08743330253847859 1103 56-57 -0.05309599335139126 1103 58-59 -0.05247959996295748 1103 60-61 -0.25360938926713317 1103 62-63 -0.19731802286667488 1103 64-65 -0.1981514971449414 1103 66-67 -0.22914164177824148 1103 68-69 -0.29110076786685113 1103 70-71 -0.2755697522570699 1103 72-73 -0.1618565939604899 1103 74-75 0.03558822883105606 1103 76-77 -0.1711825701840084 1103 78-79 -0.09254333044979646 1103 80-81 0.0053839078872464086 1103 82-83 -0.09674194678615322 1103 84-85 -0.10144032048486551 1103 86-87 -0.2448390818733266 1103 88-89 -0.052301172188855105 1103 90-91 -0.06775855172832834 1103 92-93 -0.09341481510000804 1103 94-95 -0.1898031114976284 1103 96-97 0.15894141678220564 1103 98-99 0.13687826143702253 1103 100-101 0.27756823572764944 1104 1 0.23231743838341146 1104 2 0.20510710781899633 1104 3 0.1639092709307377 1104 4 0.17303046096445485 1104 5 0.07848030219716406 1104 6 0.09800197884116812 1104 7 0.06405872999035012 1104 8 0.2598643514853265 1104 9 0.2217958545537897 1104 10-11 0.27856954796340716 1104 12-13 0.23033033831464422 1104 14-15 0.26835918825673133 1104 16-17 0.19758528526589458 1104 18-19 0.18351564890213723 1104 20-21 0.22138725639832302 1104 22-23 0.1499583226478407 1104 24-25 0.24261177972858405 1104 26-27 0.08032671005626213 1104 28-29 0.11322121638292515 1104 30-31 0.31251111568503376 1104 32-33 0.15652044235577023 1104 34-35 0.13298955847891136 1104 36-37 0.15613501846536337 1104 38-39 0.1278410641295622 1104 40-41 0.16515259337765542 1104 42-43 0.22188415620671975 1104 44-45 0.2134548058708674 1104 46-47 0.14823994241920957 1104 48-49 0.060126755114801256 1104 50-51 0.2754680492238464 1104 52-53 0.43026990234968565 1104 54-55 0.3259855560017044 1104 56-57 0.2728421333998483 1104 58-59 0.2795794664174096 1104 60-61 0.169422504965965 1104 62-63 0.29020405465565347 1104 64-65 0.25290449853604713 1104 66-67 0.3792763983973515 1104 68-69 0.3835776270636799 1104 70-71 0.17803783092908532 1104 72-73 0.38392846196923713 1104 74-75 0.5231161081919034 1104 76-77 0.1879118422180035 1104 78-79 0.23918808473464992 1104 80-81 0.5963805538138658 1104 82-83 0.6726444385585246 1104 84-85 0.5885607299532687 1104 86-87 0.6450255337518165 1104 88-89 0.5499003507021456 1104 90-91 0.5421032301567479 1104 92-93 0.412486570745461 1104 94-95 0.2900287817583127 1104 96-97 0.28376339194789324 1104 98-99 0.3917145518758396 1104 100-101 0.6074534448259108 1105 1 0.26177193387724174 1105 2 0.22370106198854955 1105 3 0.1897703822854524 1105 4 0.15731442966247755 1105 5 0.20158495101456708 1105 6 0.2496074183930972 1105 7 0.21851651412128348 1105 8 0.17890760656371185 1105 9 0.22617778078893025 1105 10-11 0.2247053262888059 1105 12-13 0.29153516340299745 1105 14-15 0.3765235587238962 1105 16-17 0.25017424655351306 1105 18-19 0.12914059226449837 1105 20-21 0.1630073705797983 1105 22-23 0.30977548904117924 1105 24-25 0.2571618064761694 1105 26-27 0.22610395385681414 1105 28-29 0.24283115479040873 1105 30-31 0.13607171576629895 1105 32-33 0.07506565161539669 1105 34-35 0.19620958730199334 1105 36-37 0.22623012585032853 1105 38-39 0.2884113284190519 1105 40-41 0.05275903559849837 1105 42-43 0.05791965302478985 1105 44-45 -0.1646971700884592 1105 46-47 0.05630865008713215 1105 48-49 0.01455099514730307 1105 50-51 -0.23541697417662988 1105 52-53 0.028865800469169756 1105 54-55 0.06359250978832165 1105 56-57 -0.04932225752995123 1105 58-59 0.08311592059427397 1105 60-61 -0.2169298703069913 1105 62-63 -0.10184204765165106 1105 64-65 -0.11573673892694103 1105 66-67 -0.11348640755551287 1105 68-69 -0.3038377818819953 1105 70-71 -0.35776848442344544 1105 72-73 -0.14467318030305165 1105 74-75 0.04839768860858129 1105 76-77 -0.18841384393785887 1105 78-79 -0.10029207674473994 1105 80-81 -0.2563813167044522 1105 82-83 0.02298696838280989 1105 84-85 -0.07461998138624537 1105 86-87 -0.11928369167913999 1105 88-89 -0.21244957782480967 1105 90-91 -0.1330079257772674 1105 92-93 0.00962633343741004 1105 94-95 -0.5322689816761361 1105 96-97 -0.21781588466887314 1105 98-99 -0.22139848662021677 1105 100-101 0.27838218517558744 1106 1 0.14719616234025779 1106 2 0.11312386918802986 1106 3 0.11670536627923411 1106 4 0.164955421214799 1106 5 0.11620288443619131 1106 6 0.14393579487152408 1106 7 0.07267379495173998 1106 8 0.19465116030056606 1106 9 0.16547486736870098 1106 10-11 0.09928434497094685 1106 12-13 0.14820770666568706 1106 14-15 0.2682665446353596 1106 16-17 0.2252706833642364 1106 18-19 -0.06366545116564026 1106 20-21 0.06942796258025652 1106 22-23 0.0830308285548611 1106 24-25 0.07435433498219624 1106 26-27 0.17156721606463776 1106 28-29 0.1462443123585473 1106 30-31 0.13499089245349438 1106 32-33 0.24012220464332046 1106 34-35 0.2223661064983986 1106 36-37 0.17116466442419664 1106 38-39 0.24366617831084625 1106 40-41 0.23403431360342353 1106 42-43 0.20232368288491642 1106 44-45 -0.08622038717626879 1106 46-47 0.04384879313153078 1106 48-49 0.09703613223293672 1106 50-51 0.11555011405221194 1106 52-53 0.1718409164568513 1106 54-55 0.0429790883683836 1106 56-57 0.050042934094122415 1106 58-59 0.094483053705126 1106 60-61 0.03327201371723021 1106 62-63 0.0736151452848759 1106 64-65 -0.21367174162156033 1106 66-67 -0.011826963378368305 1106 68-69 0.015227380330756546 1106 70-71 -0.0802676301352534 1106 72-73 -0.12042499640923054 1106 74-75 0.3091600196847786 1106 76-77 0.09126395765987638 1106 78-79 0.2849651192519218 1106 80-81 0.33271839105248446 1106 82-83 0.3130977113330182 1106 84-85 0.5472907680242507 1106 86-87 0.29232255712017974 1106 88-89 0.36131119428170777 1106 90-91 0.3099953264681048 1106 92-93 0.426853458489898 1106 94-95 0.27372429622338856 1106 96-97 0.23968561564819169 1106 98-99 -0.07053119285218656 1106 100-101 0.18684031825413427 1107 1 0.07412296039902344 1107 2 0.13558887550795617 1107 3 0.09570915823654502 1107 4 0.06849653196179872 1107 5 0.09086660180273043 1107 6 0.05700946495563386 1107 7 0.096619303120832 1107 8 0.04273684605185224 1107 9 0.059083546737646486 1107 10-11 0.1512150442203577 1107 12-13 0.031631352562413895 1107 14-15 0.187153964075776 1107 16-17 0.23044268685971048 1107 18-19 0.119818273066997 1107 20-21 -0.03280859001559122 1107 22-23 0.21701136941592836 1107 24-25 0.29224753598713704 1107 26-27 0.1974330853279369 1107 28-29 0.21870600266643692 1107 30-31 0.09724004589512703 1107 32-33 0.21664581464909816 1107 34-35 0.2169668586479503 1107 36-37 0.25421418613822055 1107 38-39 0.14710975929175873 1107 40-41 0.2219474496925642 1107 42-43 0.2772099631776612 1107 44-45 0.26632045828976914 1107 46-47 0.19387102991819916 1107 48-49 0.2609085711012824 1107 50-51 0.2706936388559882 1107 52-53 0.19600684189729378 1107 54-55 0.17880391710610866 1107 56-57 0.0569421897469482 1107 58-59 0.2647631982052232 1107 60-61 0.4309535361178618 1107 62-63 0.40362271359472146 1107 64-65 0.17110561742841668 1107 66-67 0.33083801797394585 1107 68-69 0.2496274942455159 1107 70-71 0.4343316005499531 1107 72-73 0.30784478652852343 1107 74-75 0.29537321195105903 1107 76-77 0.3521082923508203 1107 78-79 0.603024315918745 1107 80-81 0.633086663449216 1107 82-83 0.5378843162048241 1107 84-85 0.45354905805437085 1107 86-87 0.33632896287521774 1107 88-89 0.621999481346915 1107 90-91 0.5817776244254418 1107 92-93 0.7471782716228681 1107 94-95 0.9595077723402987 1107 96-97 1.0178151507823152 1107 98-99 0.8350102533972112 1107 100-101 0.7386013157105573 1108 1 -0.03590181542895721 1108 2 -0.027184007266278343 1108 3 -0.059443311552534794 1108 4 -0.004303998011209842 1108 5 0.05890311743529253 1108 6 0.008922208099207296 1108 7 0.010244367726677694 1108 8 0.0024094687619395927 1108 9 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-0.6450592449732824 2316 58-59 -0.46647663919315363 2316 60-61 -0.25487860161528175 2316 62-63 -0.9416451008481275 2316 64-65 -0.7331399588508773 2316 66-67 -0.7905488782479608 2316 68-69 -1.0292402723733218 2316 70-71 -0.9416428136059594 2316 72-73 -0.5852697519698076 2316 74-75 -0.4260471610762018 2316 76-77 -0.540869947725696 2316 78-79 -1.1084570327182703 2316 80-81 -1.1775254838487257 2316 82-83 -0.8206044451533749 2316 84-85 -0.8061257386935985 2316 86-87 -1.0389950447766232 2316 88-89 -0.9776884670031549 2316 90-91 -1.035211854292875 2316 92-93 -1.2238092941351404 2316 94-95 -0.9935637245511408 2316 96-97 -0.7201834144974555 2316 98-99 -1.1267556480853358 2316 100-101 -1.2144355087753347 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 1.0 5 21.0 6 41.0 7 89.0 8 248.0 9 469.0 10 833.0 11 1393.0 12 2112.0 13 2934.0 14 3786.0 15 5339.0 16 7195.0 17 10067.0 18 9431.0 19 6027.0 20 5224.0 21 5268.0 22 5348.0 23 6024.0 24 6820.0 25 8095.0 26 9679.0 27 11943.0 28 14385.0 29 18107.0 30 22392.0 31 28234.0 32 35689.0 33 47615.0 34 64930.0 35 95157.0 36 148322.0 37 218139.0 38 148129.0 39 9683.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42355350785166 20.432555432962147 15.388786804963155 21.755104254223042 2 15.613671267754537 21.51939119256069 39.7248105296412 23.14212701004357 3 19.040479916427483 26.09837494409211 30.265011202428777 24.59613393705163 4 13.148555521961697 17.992952239957464 37.60647226247693 31.252019975603908 5 13.469131155966977 38.280661112564914 34.015936678652714 14.2342710528154 6 30.680107425988247 37.82293478124883 17.23726297264724 14.259694820115683 7 27.626327707647846 33.88505919689065 21.103097254715525 17.385515840745978 8 26.38807221228309 35.26406108602003 19.568127509977355 18.779739191719525 9 26.60377909280087 17.397818118126885 20.681275099773554 35.31712768929869 10-11 24.504775892029024 27.468430522123526 27.352011474027808 20.67478211181964 12-13 25.698831909188343 25.17129357404094 27.824675866267988 21.305198650502724 14-15 22.85017702768638 26.53799318578941 26.37952317206924 24.23230661445497 16-17 21.580071144695633 29.597767657938306 26.929216032161072 21.89294516520499 18-19 21.44732651249494 28.110545748739664 29.866493739108407 20.575633999656993 20-21 22.46743640148192 27.018651186479026 30.084714617924092 20.429197794114966 22-23 22.10753872001743 26.903185751304655 29.989370924500204 20.99990460417771 24-25 21.952578813740466 27.187208374823395 29.99624634409555 20.863966467340585 26-27 21.727439452247324 27.20215114078649 30.145842173911685 20.9245672330545 28-29 21.469440181490626 27.365070561152997 30.151965036364846 21.013524220991524 30-31 21.77281546717323 27.19897283089765 30.00841377857896 21.019797923350165 32-33 21.739261894360624 27.431862800806144 29.88309209065289 20.945783214180334 34-35 21.892319625677146 27.32403573081783 29.87336994824703 20.910274695257993 36-37 22.10829561556339 27.16550046554207 29.890198801581068 20.836005117313473 38-39 21.741243031519193 27.596409156764945 29.77450092565511 20.887846886060746 40-41 21.90720252219641 27.412309052466522 29.7399089206851 20.940579504651968 42-43 21.769557493338006 27.700037115345317 29.28937667071182 21.241028720604856 44-45 21.909834732456744 27.73418114790673 29.198412797704687 21.157571321931833 46-47 22.357130458036398 27.974119304127893 28.574950960230737 21.093799277604973 48-49 22.276831328064723 28.287945451133307 28.288414621431702 21.14680859937027 50-51 22.100927384186456 28.4901764214304 28.2756513819531 21.133244812430043 52-53 22.365540278490197 28.396992405950133 27.96239881898137 21.2750684965783 54-55 22.10129741767591 28.529582874587128 27.919711073299304 21.44940863443766 56-57 22.06844924692483 28.75719138306014 27.89146250680301 21.282896863212024 58-59 22.203427027235538 28.18578989427833 28.00250633490744 21.60827674357869 60-61 21.936286328370784 28.55615463892267 28.342111065990167 21.165447966716382 62-63 21.870938649331286 28.459651711078156 28.543113852056706 21.126295787533852 64-65 21.90551950302944 28.495321222534237 28.362967415107725 21.2361918593286 66-67 21.995040366743872 28.51101008347552 28.037097681420974 21.456851868359635 68-69 21.7935344973636 28.505500864030527 28.32664784826425 21.374316790341624 70-71 22.06539020919237 28.4658009831468 28.007642063669962 21.461166743990866 72-73 21.99928688493826 28.545662300400764 27.87930631836626 21.57574449629472 74-75 21.980520699102975 29.106614871993763 27.498039976146092 21.41482445275717 76-77 22.196592477678664 28.618694040276406 27.706865921857606 21.477847560187328 78-79 22.10356921678243 28.820638359540673 27.414283122694567 21.66150930098233 80-81 22.176950396942274 29.056414335900378 27.224938473817033 21.541696793340314 82-83 22.240603197212163 28.936063055839117 27.421970067454065 21.401363679494654 84-85 21.895022422506546 29.203372812098248 27.197494267950255 21.70411049744495 86-87 21.742446437713024 29.104269864886138 27.35813253231879 21.79515116508205 88-89 21.993691423996047 28.84362060759792 27.246302925288045 21.916385043117987 90-91 21.921757865056374 28.959661204789384 27.316093658693223 21.80248727146102 92-93 21.782467246438458 29.166451637117696 27.170143368671155 21.880937747772684 94-95 21.7397401091775 29.083365652875603 27.174114757311514 22.002779480635382 96-97 21.775952082448217 29.193267980159465 27.27166572398927 21.75911421340305 98-99 21.499366888338287 29.607992063818905 26.86874805492146 22.023892992921347 100-101 21.33028694630456 29.942689974342372 26.671942066517996 22.055081012835068 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 335.0 1 245.0 2 181.0 3 205.0 4 202.5 5 215.5 6 363.0 7 721.5 8 1186.0 9 1485.5 10 1445.0 11 1394.5 12 1507.5 13 1595.0 14 1695.0 15 1791.0 16 1873.0 17 1939.0 18 2002.5 19 2114.0 20 2303.5 21 2547.5 22 3038.0 23 3836.0 24 4492.0 25 5373.0 26 6878.5 27 8592.5 28 10022.0 29 11598.5 30 13554.0 31 15741.0 32 17887.0 33 19752.0 34 22228.5 35 24446.0 36 27380.0 37 29855.0 38 30581.5 39 30848.0 40 30349.5 41 30229.5 42 29686.5 43 30323.5 44 32535.0 45 33263.0 46 32972.5 47 33273.5 48 35164.5 49 34415.0 50 31750.0 51 30572.5 52 29557.5 53 28987.5 54 28512.0 55 27245.5 56 24348.0 57 21739.0 58 20365.0 59 18217.0 60 15005.5 61 12159.0 62 9891.0 63 7547.0 64 5276.0 65 3629.0 66 2591.5 67 1954.0 68 1415.5 69 972.0 70 696.0 71 452.0 72 276.0 73 188.5 74 131.0 75 81.5 76 49.5 77 24.0 78 16.0 79 11.5 80 5.0 81 2.5 82 2.5 83 1.5 84 0.5 85 0.5 86 0.5 87 1.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027732252401029223 2 0.0053170859866634975 3 5.212829398689703E-4 4 2.085131759475881E-4 5 1.0425658797379406E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.014231024258422889 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.648980579082792E-4 20-21 0.0010946941737248377 22-23 0.0010946941737248377 24-25 0.011155454913195965 26-27 0.005525599162611085 28-29 4.170263518951762E-4 30-31 0.003231954227187616 32-33 0.0042745201069255565 34-35 0.0 36-37 0.007819244098034554 38-39 0.01266717543881598 40-41 8.340527037903524E-4 42-43 0.0 44-45 0.0 46-47 1.5638488196069108E-4 48-49 0.0033362108151614098 50-51 0.007975628979995245 52-53 0.0 54-55 0.0037532371670565864 56-57 0.0039617503430041745 58-59 2.6064146993448514E-4 60-61 0.0022415166414365725 62-63 0.005681984044571777 64-65 5.212829398689703E-5 66-67 0.002971312757253131 68-69 4.691546458820733E-4 70-71 7.297961158165584E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.002710671287318646 80-81 0.002867056169279337 82-83 0.015534231608095314 84-85 0.00948734950561526 86-87 0.005734112338558674 88-89 1.5638488196069108E-4 90-91 8.861809977772496E-4 92-93 1.5638488196069108E-4 94-95 0.0 96-97 0.002554286405357955 98-99 0.0021372600534627782 100-101 3.1276976392138217E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 959172.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.870606330409416 #Duplication Level Percentage of deduplicated Percentage of total 1 79.64407667822103 46.886950846724915 2 11.511947945451007 13.554307111856238 3 3.6391840676545097 6.4272291783236 4 1.6376804800624267 3.856449713470041 5 0.8988159681184965 2.6456920511294912 6 0.5727181895472268 2.022976024505975 7 0.3663991461513807 1.5099097925013256 8 0.2735604662545507 1.2883736413147924 9 0.2019007842423702 1.0697419428240156 >10 1.1363491318946877 12.111153358654773 >50 0.0787228379179156 3.1396331814266807 >100 0.03740343240047593 3.8516606112725116 >500 5.318023216103368E-4 0.23511951365961065 >1k 5.318023216103368E-4 0.8686794636940075 >5k 1.7726744053677895E-4 0.5321235686419683 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5099 0.5316043420783759 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4072 0.42453282622928945 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2694 0.28086724800140117 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1558 0.16243176406317114 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 988 0.10300550891810853 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.2128293986897034E-5 0.0 12-13 2.0851317594758814E-4 0.0 0.0 1.0425658797379407E-4 0.0 14-15 2.6064146993448514E-4 0.0 0.0 1.0425658797379407E-4 0.0 16-17 3.1276976392138217E-4 0.0 0.0 1.0425658797379407E-4 0.0 18-19 3.1276976392138217E-4 0.0 0.0 1.0425658797379407E-4 1.0425658797379407E-4 20-21 3.1276976392138217E-4 0.0 0.0 4.170263518951762E-4 1.0425658797379407E-4 22-23 3.1276976392138217E-4 0.0 0.0 5.212829398689703E-4 1.0425658797379407E-4 24-25 3.1276976392138217E-4 0.0 0.0 9.904375857510436E-4 1.0425658797379407E-4 26-27 3.1276976392138217E-4 0.0 0.0 0.001563848819606911 3.1276976392138217E-4 28-29 3.1276976392138217E-4 0.0 0.0 0.002867056169279337 3.1276976392138217E-4 30-31 3.1276976392138217E-4 0.0 0.0 0.005629855750584879 3.1276976392138217E-4 32-33 3.1276976392138217E-4 5.2128293986897034E-5 0.0 0.008132013861955938 3.1276976392138217E-4 34-35 4.170263518951762E-4 1.0425658797379407E-4 0.0 0.010946941737248375 3.1276976392138217E-4 36-37 5.212829398689703E-4 1.0425658797379407E-4 0.0 0.013136330084698052 3.1276976392138217E-4 38-39 6.255395278427643E-4 1.0425658797379407E-4 0.0 0.016472540899859462 4.170263518951763E-4 40-41 6.255395278427643E-4 1.0425658797379407E-4 0.0 0.020486419536850535 4.170263518951763E-4 42-43 7.297961158165584E-4 1.0425658797379407E-4 0.0 0.03310146668167961 4.170263518951763E-4 44-45 8.340527037903526E-4 1.0425658797379407E-4 0.0 0.04764526070402389 4.170263518951763E-4 46-47 9.904375857510436E-4 1.0425658797379407E-4 0.0 0.05921774196911503 4.170263518951763E-4 48-49 0.0010425658797379406 1.0425658797379407E-4 0.0 0.07235407205381308 4.170263518951763E-4 50-51 0.0010425658797379406 1.0425658797379407E-4 0.0 0.09205856718086015 5.212829398689703E-4 52-53 0.0010425658797379406 1.0425658797379407E-4 0.0 0.1297473237333867 5.212829398689703E-4 54-55 0.0010425658797379406 1.0425658797379407E-4 0.0 0.15497741802304488 5.212829398689703E-4 56-57 0.0010425658797379406 1.0425658797379407E-4 0.0 0.20465568219255775 5.212829398689703E-4 58-59 0.0010425658797379406 1.0425658797379407E-4 0.0 0.2727352341394453 5.212829398689703E-4 60-61 0.0010425658797379406 1.0425658797379407E-4 0.0 0.3353413152177086 5.212829398689703E-4 62-63 0.0010425658797379406 1.0425658797379407E-4 0.0 0.4257317769909881 5.212829398689703E-4 64-65 0.0010425658797379406 1.0425658797379407E-4 0.0 0.48583569995788034 5.212829398689703E-4 66-67 0.0011468224677117346 1.0425658797379407E-4 0.0 0.6038541575442152 5.212829398689703E-4 68-69 0.0011468224677117346 1.0425658797379407E-4 0.0 0.7429324459012565 5.212829398689703E-4 70-71 0.0012510790556855287 1.0425658797379407E-4 0.0 0.8389006351311339 5.212829398689703E-4 72-73 0.0012510790556855287 1.0425658797379407E-4 0.0 1.009047386704366 7.297961158165585E-4 74-75 0.0012510790556855287 1.0425658797379407E-4 0.0 1.1665269628387818 7.297961158165585E-4 76-77 0.0012510790556855287 1.0425658797379407E-4 0.0 1.2699494981087855 7.297961158165585E-4 78-79 0.0012510790556855287 1.0425658797379407E-4 0.0 1.3858828239356447 7.297961158165585E-4 80-81 0.0012510790556855287 1.563848819606911E-4 0.0 1.6090961787875377 7.297961158165585E-4 82-83 0.001355335643659323 2.0851317594758814E-4 0.0 1.7775226966592017 7.297961158165585E-4 84-85 0.00140746393764622 2.0851317594758814E-4 0.0 2.065218751172887 7.297961158165585E-4 86-87 0.001459592231633117 2.0851317594758814E-4 0.0 2.464417226524544 7.819244098034555E-4 88-89 0.001459592231633117 2.0851317594758814E-4 0.0 2.997429032540566 8.340527037903526E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6400 0.0 29.093605 1 GGTATCA 4540 0.0 28.876509 1 CCGTCCA 135 1.3413939E-5 24.632074 9 TATCAAC 7860 0.0 23.867016 2 ATCAACG 7830 0.0 23.83715 3 ACTGCGT 120 1.7093768E-4 23.75236 8 TCAACGC 7875 0.0 23.640629 4 CAACGCA 7905 0.0 23.557053 5 AACGCAG 8080 0.0 23.046844 6 GTACATG 4060 0.0 22.696934 1 TATACCG 105 0.002194691 22.621294 5 GAGTACT 4705 0.0 22.206831 12-13 ACATGGG 3970 0.0 21.891933 3 TACATGG 4170 0.0 21.866976 2 TACGGTG 110 0.0028771053 21.590803 1 GTACTTT 4830 0.0 21.337135 14-15 TAAGGTG 405 0.0 21.113209 5 AGTACTT 4940 0.0 20.669743 12-13 ACGCAGA 8970 0.0 20.654226 7 CGCAGAG 8990 0.0 20.502592 8 >>END_MODULE