FastQCFastQC Report
Thu 26 May 2016
SRR937068_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937068_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019384
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77900.7641869992073644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55790.547291305337341No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35570.34893622030559635No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12750.12507553581378558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34650.042.3847051
GTATCAA60800.041.9001051
ATCAACG76850.032.7798733
TATCAAC77700.032.6047942
TCAACGC77800.032.442294
CAACGCA78050.032.3383755
AACGCAG80200.031.4668126
CGCAGAG87450.028.693628
ACGCAGA88100.028.6423447
GCAGAGT91300.026.9084329
GTACATG43350.025.2169551
TACATGG43150.024.3437082
GAGTACT55000.024.27677212-13
ACATGGG43700.023.4934923
AGAGTAC79050.023.29312710-11
AGTACTT58650.022.44187212-13
CAGGACG1703.1293985E-622.3685384
GTACTTT60600.021.95388614-15
CAGAGTA89900.021.22187810-11
ATGGGTA3750.020.280815
Produced by FastQC (version 0.11.5)