##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937068_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1019384 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75029920030136 33.0 31.0 34.0 30.0 34.0 2 31.836502240568816 33.0 31.0 34.0 30.0 34.0 3 31.846570085463377 33.0 31.0 34.0 30.0 34.0 4 35.440284524771826 37.0 35.0 37.0 33.0 37.0 5 35.44352765984163 37.0 35.0 37.0 33.0 37.0 6 35.51202785211461 37.0 35.0 37.0 33.0 37.0 7 35.45965014165417 37.0 35.0 37.0 33.0 37.0 8 35.4233733313452 37.0 35.0 37.0 33.0 37.0 9 37.048983503763054 39.0 37.0 39.0 33.0 39.0 10-11 36.9183786482817 39.0 37.0 39.0 33.0 39.0 12-13 36.83748665860951 39.0 37.0 39.0 33.0 39.0 14-15 37.975303712830495 40.0 38.0 41.0 33.0 41.0 16-17 37.915397436098665 40.0 38.0 41.0 33.0 41.0 18-19 37.90453254122097 40.0 38.0 41.0 33.0 41.0 20-21 37.84332989334735 40.0 38.0 41.0 32.5 41.0 22-23 37.73960744920461 40.0 37.5 41.0 32.0 41.0 24-25 37.695832973638986 40.0 37.5 41.0 32.0 41.0 26-27 37.70306184911672 40.0 37.0 41.0 32.0 41.0 28-29 37.61557273804572 40.0 37.0 41.0 32.0 41.0 30-31 37.43087099660187 40.0 37.0 41.0 32.0 41.0 32-33 37.18909557144315 40.0 36.0 41.0 31.0 41.0 34-35 37.00752464233302 39.5 36.0 41.0 31.0 41.0 36-37 36.818897000541504 39.0 36.0 41.0 30.5 41.0 38-39 36.640808566742265 39.0 35.0 41.0 30.0 41.0 40-41 36.45267337921725 39.0 35.0 41.0 30.0 41.0 42-43 36.20371763731822 39.0 35.0 41.0 30.0 41.0 44-45 35.90408570273812 39.0 35.0 41.0 28.5 41.0 46-47 35.49261465747942 39.0 35.0 40.0 26.5 41.0 48-49 35.18666322014079 39.0 35.0 40.0 25.5 41.0 50-51 34.470392903949836 38.0 34.0 40.0 24.5 40.5 52-53 34.6508464916067 38.5 34.5 40.0 24.5 40.5 54-55 35.028101775189725 39.0 35.0 40.5 25.0 41.0 56-57 34.9062051199548 39.0 35.0 41.0 24.5 41.0 58-59 34.68938937632923 38.5 34.5 40.5 24.0 41.0 60-61 34.41390290606876 38.0 34.0 40.0 23.0 41.0 62-63 34.1064814633151 38.0 34.0 40.0 22.5 41.0 64-65 33.780279560989776 37.0 34.0 40.0 22.0 41.0 66-67 33.40553952190735 37.0 33.0 40.0 20.5 41.0 68-69 33.01810259921678 36.0 33.0 39.0 19.5 41.0 70-71 32.60174428870769 36.0 32.5 39.0 18.0 41.0 72-73 32.21630563163636 35.0 32.0 38.5 17.0 40.0 74-75 31.81102067523132 35.0 32.0 37.5 12.5 40.0 76-77 31.36625256036979 35.0 31.0 37.0 9.0 39.0 78-79 30.90988773612299 35.0 31.0 36.5 7.0 39.0 80-81 30.484116387936243 35.0 31.0 36.0 4.0 38.5 82-83 30.087090340833285 34.5 30.0 35.5 2.0 37.0 84-85 29.704613766745407 34.0 30.0 35.0 2.0 37.0 86-87 29.368834511822826 34.0 30.0 35.0 2.0 36.5 88-89 29.004579236087675 34.0 29.0 35.0 2.0 36.0 90-91 28.63254769547099 34.0 29.0 35.0 2.0 36.0 92-93 28.26562414163848 34.0 29.0 35.0 2.0 35.0 94-95 27.90816365569795 34.0 29.0 35.0 2.0 35.0 96-97 27.443871494942044 34.0 27.0 35.0 2.0 35.0 98-99 26.89952265289626 34.0 26.5 35.0 2.0 35.0 100-101 24.780676859750592 31.5 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3194808340084556 1101 2 0.30022712858495026 1101 3 0.3048406122077836 1101 4 0.3601627460651642 1101 5 0.25034671069104064 1101 6 0.14757374086902786 1101 7 0.25677529530157983 1101 8 0.19367213541731587 1101 9 0.17166975953771413 1101 10-11 0.20393651463005824 1101 12-13 0.2784702784241233 1101 14-15 0.3969819844832685 1101 16-17 0.481106894081492 1101 18-19 0.46587779878628766 1101 20-21 0.38073640767499484 1101 22-23 0.44943112907700566 1101 24-25 0.47688729716860223 1101 26-27 0.4922125098474339 1101 28-29 0.48466741922431567 1101 30-31 0.410261921994838 1101 32-33 0.5854747202594694 1101 34-35 0.5563233110575894 1101 36-37 0.3916535380109636 1101 38-39 0.4315082360794662 1101 40-41 0.6241500083311706 1101 42-43 0.6271693813830908 1101 44-45 0.6019647033165327 1101 46-47 0.6446644272095341 1101 48-49 0.8173309530212052 1101 50-51 0.8767994408228148 1101 52-53 0.7372311328782999 1101 54-55 0.7699562055557791 1101 56-57 0.577997646046768 1101 58-59 0.7584714779395938 1101 60-61 0.8091367918551313 1101 62-63 0.8023918739439964 1101 64-65 0.7162188674874628 1101 66-67 0.7751142191447897 1101 68-69 0.731698243900361 1101 70-71 0.8595149767842116 1101 72-73 0.6646823367309835 1101 74-75 0.7859692899163484 1101 76-77 0.7313949987722417 1101 78-79 0.7005628311305117 1101 80-81 0.6913815293314904 1101 82-83 0.8669255664902238 1101 84-85 0.5076347657564213 1101 86-87 0.8139360159985785 1101 88-89 0.8398383286611413 1101 90-91 0.8189679569774597 1101 92-93 0.6884312029669175 1101 94-95 0.8283817298097667 1101 96-97 0.8511924226723444 1101 98-99 0.8208004118656014 1101 100-101 0.9941340304665758 1102 1 0.12850285752934099 1102 2 0.10635389127480721 1102 3 0.23394091434512632 1102 4 0.16850802456971792 1102 5 0.19442554704485815 1102 6 0.1373750513742067 1102 7 0.02447723974454874 1102 8 0.04947748863025225 1102 9 0.2039092881963498 1102 10-11 0.2730875104754489 1102 12-13 0.14597657073178283 1102 14-15 0.0834756371123575 1102 16-17 0.2557943213669276 1102 18-19 0.200616177333643 1102 20-21 0.11817199700266912 1102 22-23 0.13663416501553627 1102 24-25 -0.04108885334233037 1102 26-27 0.17192206012283862 1102 28-29 -0.0019777461341590197 1102 30-31 0.030173900736876647 1102 32-33 0.012611618138464564 1102 34-35 0.10932701095226349 1102 36-37 0.06781461753471518 1102 38-39 0.14708993483379373 1102 40-41 0.05636649035834296 1102 42-43 0.042715466652957446 1102 44-45 0.10986139401563833 1102 46-47 0.31051225016877737 1102 48-49 0.445414272588728 1102 50-51 0.27840098188311657 1102 52-53 0.5241961091034213 1102 54-55 0.24848131883507563 1102 56-57 0.37515465089055766 1102 58-59 0.29715917876500697 1102 60-61 0.25510487471288457 1102 62-63 0.13657047246013576 1102 64-65 0.4456554106526056 1102 66-67 0.09578478286805847 1102 68-69 0.301949394455697 1102 70-71 0.18171398955355045 1102 72-73 0.1587370607131504 1102 74-75 0.25002653615028336 1102 76-77 0.2557094169935574 1102 78-79 0.2027218431183151 1102 80-81 0.1201596670926186 1102 82-83 0.2618542418284626 1102 84-85 0.21779828582519656 1102 86-87 0.10178666672177172 1102 88-89 0.25266611037623576 1102 90-91 0.13769576515843696 1102 92-93 0.38004706935151944 1102 94-95 0.24302704259563157 1102 96-97 0.1659978629207295 1102 98-99 0.2476537784500259 1102 100-101 0.49341354569689244 1103 1 0.1700607572849897 1103 2 0.19703271599618688 1103 3 0.26107900362338654 1103 4 0.2052146221023463 1103 5 0.055764125029241995 1103 6 0.019289944991228936 1103 7 0.1964272963629412 1103 8 0.33214630083598706 1103 9 0.1284518711869964 1103 10-11 0.10371210073708426 1103 12-13 0.04394426844917376 1103 14-15 0.27353612939437966 1103 16-17 0.4955881112232987 1103 18-19 0.2884774920723032 1103 20-21 0.30621210666355836 1103 22-23 0.41342956402620956 1103 24-25 0.31647566182543585 1103 26-27 0.5481214760594284 1103 28-29 0.4449097322614577 1103 30-31 0.44261057753525535 1103 32-33 0.4800456130130115 1103 34-35 0.5616334654489563 1103 36-37 0.2064909391271854 1103 38-39 0.3891171712602812 1103 40-41 0.4378120473045328 1103 42-43 0.2519457545127821 1103 44-45 0.4525010030507417 1103 46-47 0.44350885306526067 1103 48-49 0.5763308945566621 1103 50-51 0.5180785550400415 1103 52-53 0.4819274995319276 1103 54-55 0.5675632069094618 1103 56-57 0.5841227061977889 1103 58-59 0.5125891805529577 1103 60-61 0.5584736690391239 1103 62-63 0.5658079121168456 1103 64-65 0.5514304866404487 1103 66-67 0.42679080229709854 1103 68-69 0.40113140768652045 1103 70-71 0.368426546916929 1103 72-73 0.23228286201239 1103 74-75 0.42957686692089325 1103 76-77 0.3626287804836359 1103 78-79 0.5471494013756626 1103 80-81 0.6172765989717561 1103 82-83 0.5677276550235284 1103 84-85 0.5743987983706411 1103 86-87 0.6246336778666546 1103 88-89 0.4867921011980947 1103 90-91 0.5285682843788919 1103 92-93 0.7552824826657805 1103 94-95 0.8759968262537718 1103 96-97 0.7427843221852868 1103 98-99 0.6151280021820718 1103 100-101 0.8313165792507355 1104 1 0.23715360159024357 1104 2 0.21933170759101728 1104 3 0.1737115115065997 1104 4 0.30555039842365517 1104 5 0.311337258618714 1104 6 0.17999012471765496 1104 7 0.18800167835155435 1104 8 0.20062960349744685 1104 9 0.17358365263105213 1104 10-11 0.35603616793539317 1104 12-13 0.16843441578179608 1104 14-15 0.01807276883302933 1104 16-17 0.3049435713552384 1104 18-19 0.4869207598646881 1104 20-21 0.43877649069695934 1104 22-23 0.3674960089944008 1104 24-25 0.1953491968397998 1104 26-27 0.19521195271113356 1104 28-29 0.2095063163831128 1104 30-31 0.3848328356310091 1104 32-33 0.24946091020734684 1104 34-35 0.30093597623791624 1104 36-37 0.28884384865053647 1104 38-39 0.2642465850507847 1104 40-41 0.46857378602319955 1104 42-43 0.5403388710981929 1104 44-45 0.32763256925356643 1104 46-47 0.39623864998515756 1104 48-49 0.2761834543130135 1104 50-51 0.5589603983659472 1104 52-53 0.6045003855939797 1104 54-55 0.7587915886182657 1104 56-57 0.7742840295008762 1104 58-59 0.6489208242426301 1104 60-61 0.7315211620168824 1104 62-63 0.5936488273225606 1104 64-65 0.6292127486105485 1104 66-67 0.4799962823388526 1104 68-69 0.5392673110001596 1104 70-71 0.5658447283437553 1104 72-73 0.7024461224865703 1104 74-75 0.8913710102069778 1104 76-77 0.9514265505844755 1104 78-79 0.932434972679701 1104 80-81 0.8109721832978884 1104 82-83 0.758902226424425 1104 84-85 0.8550744405966668 1104 86-87 0.8244544895413384 1104 88-89 0.7438961380473934 1104 90-91 0.8911238435218003 1104 92-93 0.7582721833728492 1104 94-95 0.9751385518427291 1104 96-97 1.0006201792220644 1104 98-99 0.959601683560475 1104 100-101 1.1971212047848034 1105 1 0.13233390077262186 1105 2 0.0986974205068698 1105 3 0.0756905251758262 1105 4 0.06674783372513104 1105 5 0.044029863510822054 1105 6 0.06566962501761253 1105 7 0.06209525407136596 1105 8 0.05432946951884077 1105 9 0.1535890799205788 1105 10-11 0.15113712647261224 1105 12-13 0.0611657418292495 1105 14-15 0.28860520297238423 1105 16-17 0.4399595672567784 1105 18-19 0.13394889293793 1105 20-21 0.18331573693649972 1105 22-23 0.1948607435378804 1105 24-25 0.13310877278109245 1105 26-27 0.2883505080055997 1105 28-29 0.39870181197617427 1105 30-31 0.4052502745101165 1105 32-33 0.21542413849545028 1105 34-35 0.0039020021355824497 1105 36-37 0.00698346871593003 1105 38-39 0.39950778248609 1105 40-41 0.2991589962941603 1105 42-43 0.3981980365855051 1105 44-45 0.2262319999965996 1105 46-47 0.31686362792918743 1105 48-49 0.29535328588450227 1105 50-51 0.19460491333541086 1105 52-53 0.4062966237278687 1105 54-55 0.3576494848986229 1105 56-57 0.44575686932404324 1105 58-59 0.24491783314897475 1105 60-61 0.41345157345653405 1105 62-63 0.2189988327376895 1105 64-65 0.1256588474724012 1105 66-67 0.10418477478142307 1105 68-69 0.10874087608443261 1105 70-71 0.18592016280071988 1105 72-73 0.24390252224660003 1105 74-75 0.42529339509915687 1105 76-77 0.4201213726338686 1105 78-79 0.4540297439851564 1105 80-81 0.3200711072099658 1105 82-83 0.1383780670762853 1105 84-85 0.11664515416354959 1105 86-87 0.2433168152687628 1105 88-89 0.2561096690346645 1105 90-91 0.35054329390530015 1105 92-93 0.3522394346697375 1105 94-95 0.4361892312882638 1105 96-97 0.3220912545676846 1105 98-99 0.30811428983982836 1105 100-101 0.15708338412143164 1106 1 0.008380836118039525 1106 2 0.11483399564532348 1106 3 0.10866751731508018 1106 4 0.046256384187536526 1106 5 0.07167507699382725 1106 6 0.022730563442046048 1106 7 -0.026374607843806075 1106 8 0.032440536863248326 1106 9 0.0916917984607295 1106 10-11 0.2453832780975631 1106 12-13 0.15666303709915752 1106 14-15 0.14278360687560365 1106 16-17 0.36442407463809445 1106 18-19 0.3348349333291907 1106 20-21 0.04839819707174087 1106 22-23 -0.04425874009171338 1106 24-25 0.0017673302269827218 1106 26-27 -0.22000457354432257 1106 28-29 0.0027083748896004067 1106 30-31 -0.047207051644079456 1106 32-33 -0.038580776627654245 1106 34-35 0.21175976487574388 1106 36-37 -0.048806003316364865 1106 38-39 -0.08774852109652187 1106 40-41 0.19799094836072584 1106 42-43 0.035103517763808156 1106 44-45 -0.10331154639339957 1106 46-47 0.06612408394338587 1106 48-49 0.2517052821432344 1106 50-51 0.11678016917446854 1106 52-53 -0.010544499468785773 1106 54-55 0.21198521354364885 1106 56-57 0.15950195145462231 1106 58-59 0.35298973016364954 1106 60-61 0.18473631633062126 1106 62-63 0.3600029961545417 1106 64-65 0.15162409949800804 1106 66-67 0.19138740747954586 1106 68-69 0.20473996273780415 1106 70-71 0.2211503120277527 1106 72-73 0.29567060125265954 1106 74-75 0.46542022787354753 1106 76-77 0.48538210798776404 1106 78-79 0.47098668330096416 1106 80-81 0.7013489921377065 1106 82-83 0.5757428109547433 1106 84-85 0.5731760533705703 1106 86-87 0.7105603131666527 1106 88-89 0.6873673881476172 1106 90-91 0.6421468606651324 1106 92-93 0.5731469120283172 1106 94-95 0.7089915105746023 1106 96-97 0.9062227199868467 1106 98-99 1.2203812255932682 1106 100-101 1.1777454976557529 1107 1 0.21540457473796337 1107 2 0.2677928146702513 1107 3 0.27032198851392053 1107 4 0.19170860915137666 1107 5 0.31831423295400896 1107 6 0.21491128325826026 1107 7 0.2203943022217132 1107 8 0.2956137692294476 1107 9 0.33080308919501533 1107 10-11 0.23400426848299816 1107 12-13 0.294183244705728 1107 14-15 0.27354383830587636 1107 16-17 0.4659088019417652 1107 18-19 0.4417576339162821 1107 20-21 0.5282788658371658 1107 22-23 0.6915863632861488 1107 24-25 0.6544613232500467 1107 26-27 0.6082818214186645 1107 28-29 0.5265586684316688 1107 30-31 0.5339264887285893 1107 32-33 0.36983515972901415 1107 34-35 0.2632561913200604 1107 36-37 0.2750655613905266 1107 38-39 0.5247631780441182 1107 40-41 0.4818993121827333 1107 42-43 0.44703132343044416 1107 44-45 0.4859422611450981 1107 46-47 0.49407545806062814 1107 48-49 0.3539866145689956 1107 50-51 0.26990171261019213 1107 52-53 0.5433289796429364 1107 54-55 0.6850739993855655 1107 56-57 0.631050704964359 1107 58-59 0.5132945459553682 1107 60-61 0.5201411065969381 1107 62-63 0.37402175732190557 1107 64-65 0.4729846031991869 1107 66-67 0.4716296860467182 1107 68-69 0.6939235754324358 1107 70-71 0.5363921651716268 1107 72-73 0.40374832608886635 1107 74-75 0.5530042489745419 1107 76-77 0.5258302914302853 1107 78-79 0.46827567334605646 1107 80-81 0.5130289887343231 1107 82-83 0.6273136864758868 1107 84-85 0.5816760108282395 1107 86-87 0.47448335409237075 1107 88-89 0.6415407906831412 1107 90-91 0.600870165353296 1107 92-93 0.7167995964493166 1107 94-95 0.7959012357511526 1107 96-97 0.8060706311584589 1107 98-99 0.8269072189566664 1107 100-101 0.7630503134474687 1108 1 0.11091835066097389 1108 2 0.09219876791302539 1108 3 0.20846128076789583 1108 4 0.03197603665211801 1108 5 0.21512256238380445 1108 6 0.1838164486970939 1108 7 0.13852550351626292 1108 8 0.15134663981667984 1108 9 0.009505767728420267 1108 10-11 -0.004280341660511056 1108 12-13 0.1854652359334139 1108 14-15 0.15600881296840186 1108 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-3.2131432891278315 2316 96-97 -3.6053216980029354 2316 98-99 -3.627150606694201 2316 100-101 -3.907313495370289 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2451.0 3 845.0 4 817.0 5 877.0 6 1013.0 7 1221.0 8 1472.0 9 1762.0 10 2284.0 11 2894.0 12 3748.0 13 4891.0 14 5466.0 15 6740.0 16 8871.0 17 12114.0 18 10687.0 19 6422.0 20 5978.0 21 6390.0 22 6721.0 23 7447.0 24 8141.0 25 9298.0 26 10793.0 27 12586.0 28 14873.0 29 17988.0 30 21382.0 31 26490.0 32 33761.0 33 43899.0 34 60465.0 35 87751.0 36 133105.0 37 203166.0 38 195086.0 39 39469.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18555153227943 19.002031263799346 15.409834456906788 22.402582747014435 2 14.859997311508803 20.064484540685015 41.56858743336417 23.506930714442007 3 18.254310112218437 25.40510786686853 30.979333243718195 25.361248777194845 4 12.188630173957849 17.01028044262335 38.74043722550727 32.06065215791153 5 12.998159012248706 36.901543975060754 35.16738260634772 14.932914406342817 6 29.86356820264143 37.06038724908206 18.313265790117164 14.762778758159346 7 26.656367685408654 33.45997677821824 22.11192584733494 17.77172968903816 8 25.385739824292898 34.30450989420073 20.727116010080234 19.582634271426144 9 25.82856279055158 16.648703511747705 21.58415394521925 35.93857975248147 10-11 23.756880587601568 26.683894210122038 28.3053447103834 21.253880491892993 12-13 25.21327670167372 24.521495523422402 28.76342212541485 21.50180564948903 14-15 22.493686304802534 25.349498486970674 27.60912901924487 24.547686188981928 16-17 20.76817725234719 28.65516474856259 28.273784842258777 22.302873156831446 18-19 20.7535122895166 26.650360148623577 31.58059530896418 21.015532252895643 20-21 21.500411254433992 25.79389773015035 31.776570079129673 20.92912093628599 22-23 21.22258688272188 25.608734712021512 31.827354705633276 21.34132369962333 24-25 21.0620729641713 25.76565387220846 31.806612031588084 21.36566113203216 26-27 20.820328843749433 25.907641023603546 31.87795865933461 21.394071473312408 28-29 20.825979682832436 25.83336194675941 32.07429353451025 21.266364835897914 30-31 20.944514506221438 25.72751838569818 31.809845306539486 21.5181218015409 32-33 20.788979674124768 25.827846404919374 31.896962327206513 21.486211593749342 34-35 21.13709674254702 25.855370814382866 31.5829412434123 21.424591199657815 36-37 21.171259552843537 25.756232034767933 31.591928031157725 21.48058038123081 38-39 20.89478369847526 25.981100747778612 31.563467985804717 21.560647567941412 40-41 21.003801990416296 25.91131762929637 31.694846000012756 21.39003438027458 42-43 20.817592231981077 25.91206827035421 31.38751901930527 21.882820478359438 44-45 20.950628232394976 26.031864841745083 31.21443875028449 21.803068175575454 46-47 21.284610668301656 26.306462293071736 30.73854077253219 21.67038626609442 48-49 21.298296418810615 26.48457594895731 30.31748647710083 21.899641155131246 50-51 21.292142492071076 26.67591421752084 30.211483770842552 21.820459519565535 52-53 21.39275686769256 26.851348620379106 29.828329759118173 21.92756475281016 54-55 21.289095266920636 26.789780559280977 29.872418871097338 22.048705302701045 56-57 21.273454243285947 27.038252288215865 29.7110218449257 21.977271623572488 58-59 21.363294116492902 26.71375630621019 29.880044048433657 22.04290552886325 60-61 21.32216033165843 26.655398180430506 30.2376878871294 21.784753600781663 62-63 21.146150442608107 26.687115360654722 30.414039452604193 21.752694744132977 64-65 21.078901919389654 26.715425995023352 30.16606980553004 22.039602280056958 66-67 21.051309599148354 26.78641686412449 29.900265402936604 22.26200813379055 68-69 21.04193184639678 26.734238632806097 30.196435003037404 22.02739451775972 70-71 21.25403941140531 26.622401833408173 29.991485644600502 22.132073110586017 72-73 21.209613693317905 26.720481557764025 29.809924575112678 22.25998017380539 74-75 21.085908569371085 27.117545746954576 29.576841589933238 22.219704093741093 76-77 21.191818737508672 26.91195213533659 29.6560463183632 22.24018280879154 78-79 21.136835288403518 26.86114340942653 29.55933650265664 22.44268479951332 80-81 21.214426691706258 27.026314639865273 29.372401901137142 22.38685676729133 82-83 21.40340565371684 27.057171551943654 29.428039165949937 22.11138362838957 84-85 21.219037920239074 26.93856687769985 29.248747389065322 22.59364781299576 86-87 21.042646169213885 27.01045069832409 29.512711230342692 22.434191902119334 88-89 21.31148667182557 26.737216380972384 29.33199404681582 22.61930290038623 90-91 21.13202825409054 26.71979233881355 29.618167409657325 22.530011997438585 92-93 21.105629970503905 26.672741556614486 29.578040499593456 22.64358797328815 94-95 20.964931558457867 26.59158961760643 29.590272981288457 22.85320584264724 96-97 20.834543399514143 26.723363172911263 29.692767467141795 22.7493259604328 98-99 20.836328398120777 26.992235320093716 29.399212492180123 22.772223789605388 100-101 20.669024368095933 27.068686665406826 29.388969196900796 22.87331976959645 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 391.0 1 283.0 2 247.0 3 317.5 4 261.0 5 241.5 6 472.0 7 1196.0 8 2271.5 9 2787.0 10 2274.5 11 1723.5 12 1684.0 13 1730.0 14 1826.0 15 1942.5 16 2022.0 17 2100.0 18 2195.0 19 2340.0 20 2581.5 21 2961.5 22 3520.5 23 4228.0 24 4886.0 25 5954.0 26 7815.5 27 9620.0 28 10770.5 29 12096.0 30 14072.0 31 16315.0 32 18619.0 33 21016.5 34 23475.5 35 25733.0 36 29103.5 37 31861.5 38 32033.0 39 31898.5 40 31638.5 41 31841.5 42 31823.5 43 32279.5 44 33708.0 45 34793.0 46 35329.5 47 35327.5 48 36502.5 49 36277.5 50 33988.0 51 32470.5 52 31681.5 53 31207.0 54 30275.0 55 28313.0 56 25971.5 57 23437.0 58 21530.5 59 19211.0 60 15916.0 61 12753.0 62 9915.5 63 7575.5 64 5502.5 65 3923.5 66 2832.0 67 2098.0 68 1495.5 69 1005.0 70 706.0 71 475.5 72 307.5 73 213.0 74 145.0 75 76.0 76 49.0 77 32.0 78 15.0 79 9.5 80 12.0 81 10.5 82 8.5 83 6.0 84 4.0 85 5.0 86 3.5 87 3.5 88 3.5 89 2.0 90 2.0 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03080291627100288 2 0.022072153378903336 3 0.02069877494643824 4 0.04502719289296281 5 0.08917149965076948 6 0.10525964700250348 7 0.050324509703899616 8 0.07426053381257701 9 0.06199822637985293 10-11 0.06538262323128477 12-13 0.062488718677161895 14-15 0.055621826514836416 16-17 0.044389552906461155 18-19 0.0557689742040291 20-21 0.05395415270398594 22-23 0.03173485163588991 24-25 0.007406433689365342 26-27 0.002403412256813919 28-29 0.004806824513627838 30-31 0.004463479905511564 32-33 0.006621646013671002 34-35 0.005885907567707556 36-37 0.005297316810936801 38-39 0.003090101473046467 40-41 0.004855873743358734 42-43 0.0024524614865448153 44-45 0.0015695753513886817 46-47 8.828861351561336E-4 48-49 1.9619691892358522E-4 50-51 0.001471476891926889 52-53 0.00451252913524246 54-55 0.010888929000258979 56-57 0.008387418283983268 58-59 0.005052070662282319 60-61 0.0021581661081594376 62-63 0.008338369054252372 64-65 0.0023053137973521264 66-67 0.017559624243660876 68-69 0.041299451433414694 70-71 0.050667854312015884 72-73 0.0025015107162757117 74-75 0.010643682851604499 76-77 0.017363427324737293 78-79 0.023445531811368436 80-81 0.07166092463683951 82-83 0.027909011716879997 84-85 0.011085125919182565 86-87 0.026045140987105937 88-89 0.0425256821766871 90-91 0.040858008365836626 92-93 0.04272187909561068 94-95 0.011771815135415113 96-97 0.015303359676039649 98-99 0.03399111620351114 100-101 0.07224951539361026 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1019384.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.613302623322184 #Duplication Level Percentage of deduplicated Percentage of total 1 81.272772945222 50.074839545105156 2 10.962608784866623 13.508850652061549 3 3.257979129629672 6.022045621630225 4 1.419515419686727 3.4984413252652202 5 0.7700949511931063 2.3724046638276692 6 0.475776091087772 1.7588481768679318 7 0.3284124135718003 1.416420139585848 8 0.24923454132559866 1.228493057510321 9 0.16869521764338466 0.9354482546192153 >10 0.9964303726062427 11.173685522473766 >50 0.06841942698671825 2.897149097745908 >100 0.028311141673830083 2.8819874820593636 >500 0.0011133592314635028 0.4477472438050621 >1k 3.1810263756100086E-4 0.4735203944114817 >5k 3.1810263756100086E-4 1.3101188230312704 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7790 0.7641869992073644 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5579 0.547291305337341 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3557 0.34893622030559635 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1275 0.12507553581378558 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 9.809845946179261E-5 0.0 0.0 0.0 6 0.0 9.809845946179261E-5 0.0 0.0 0.0 7 0.0 9.809845946179261E-5 0.0 0.0 0.0 8 0.0 9.809845946179261E-5 0.0 0.0 0.0 9 0.0 9.809845946179261E-5 0.0 9.809845946179261E-5 0.0 10-11 0.0 9.809845946179261E-5 0.0 9.809845946179261E-5 0.0 12-13 0.0 9.809845946179261E-5 0.0 9.809845946179261E-5 0.0 14-15 9.809845946179261E-5 9.809845946179261E-5 0.0 1.471476891926889E-4 0.0 16-17 9.809845946179261E-5 9.809845946179261E-5 0.0 1.9619691892358522E-4 0.0 18-19 9.809845946179261E-5 1.9619691892358522E-4 0.0 1.9619691892358522E-4 0.0 20-21 9.809845946179261E-5 1.9619691892358522E-4 0.0 2.4524614865448154E-4 0.0 22-23 9.809845946179261E-5 1.9619691892358522E-4 0.0 3.433446081162741E-4 4.9049229730896304E-5 24-25 9.809845946179261E-5 1.9619691892358522E-4 0.0 7.357384459634446E-4 9.809845946179261E-5 26-27 9.809845946179261E-5 1.9619691892358522E-4 0.0 0.001275279973003304 1.9619691892358522E-4 28-29 2.4524614865448154E-4 1.9619691892358522E-4 0.0 0.002844855324391986 1.9619691892358522E-4 30-31 2.9429537838537785E-4 1.9619691892358522E-4 0.0 0.005885907567707557 1.9619691892358522E-4 32-33 2.9429537838537785E-4 1.9619691892358522E-4 0.0 0.009074107500215816 1.9619691892358522E-4 34-35 2.9429537838537785E-4 1.9619691892358522E-4 0.0 0.011673716675953322 1.9619691892358522E-4 36-37 3.9239383784717044E-4 1.9619691892358522E-4 0.0 0.014910965838192477 1.9619691892358522E-4 38-39 3.9239383784717044E-4 1.9619691892358522E-4 0.0 0.01819726423016253 1.9619691892358522E-4 40-41 3.9239383784717044E-4 1.9619691892358522E-4 0.0 0.02153261185186348 1.9619691892358522E-4 42-43 4.4144306757806675E-4 1.9619691892358522E-4 0.0 0.0331572792980859 1.9619691892358522E-4 44-45 6.866892162325482E-4 2.4524614865448154E-4 0.0 0.04640057132542791 1.9619691892358522E-4 46-47 7.357384459634446E-4 2.9429537838537785E-4 0.0 0.05777999262299585 1.9619691892358522E-4 48-49 7.847876756943409E-4 2.9429537838537785E-4 0.0 0.07131758002872324 2.9429537838537785E-4 50-51 7.847876756943409E-4 2.9429537838537785E-4 0.0 0.08671903816422466 2.9429537838537785E-4 52-53 7.847876756943409E-4 2.9429537838537785E-4 0.0 0.11860103748930728 4.4144306757806675E-4 54-55 7.847876756943409E-4 2.9429537838537785E-4 0.0 0.14101653547632687 4.904922973089631E-4 56-57 7.847876756943409E-4 2.9429537838537785E-4 0.0 0.18516084223413354 4.904922973089631E-4 58-59 7.847876756943409E-4 2.9429537838537785E-4 0.0 0.24779670860048814 4.904922973089631E-4 60-61 8.338369054252372E-4 2.9429537838537785E-4 0.0 0.3073424734937963 4.904922973089631E-4 62-63 8.828861351561335E-4 2.9429537838537785E-4 0.0 0.39661207160402756 4.904922973089631E-4 64-65 8.828861351561335E-4 2.9429537838537785E-4 0.0 0.455029704213525 4.904922973089631E-4 66-67 9.809845946179261E-4 2.9429537838537785E-4 0.0 0.5740721847704104 4.904922973089631E-4 68-69 9.809845946179261E-4 2.9429537838537785E-4 0.0 0.7081727788546809 5.395415270398594E-4 70-71 9.809845946179261E-4 4.4144306757806675E-4 0.0 0.7988648046271082 5.885907567707557E-4 72-73 9.809845946179261E-4 5.885907567707557E-4 0.0 0.9591576873876773 6.37639986501652E-4 74-75 9.809845946179261E-4 5.885907567707557E-4 0.0 1.1069920657965988 6.866892162325482E-4 76-77 9.809845946179261E-4 5.885907567707557E-4 0.0 1.205188623717853 6.866892162325482E-4 78-79 9.809845946179261E-4 5.885907567707557E-4 0.0 1.307456267706772 6.866892162325482E-4 80-81 0.001275279973003304 5.885907567707557E-4 0.0 1.5010535774546196 6.866892162325482E-4 82-83 0.001275279973003304 5.885907567707557E-4 0.0 1.6587959002691823 7.847876756943409E-4 84-85 0.001275279973003304 5.885907567707557E-4 0.0 1.9280761714918029 7.847876756943409E-4 86-87 0.001275279973003304 6.866892162325482E-4 0.0 2.3089434403522127 7.847876756943409E-4 88-89 0.001275279973003304 6.866892162325482E-4 0.0 2.8149352942561388 7.847876756943409E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3465 0.0 42.384705 1 GTATCAA 6080 0.0 41.900105 1 ATCAACG 7685 0.0 32.779873 3 TATCAAC 7770 0.0 32.604794 2 TCAACGC 7780 0.0 32.44229 4 CAACGCA 7805 0.0 32.338375 5 AACGCAG 8020 0.0 31.466812 6 CGCAGAG 8745 0.0 28.69362 8 ACGCAGA 8810 0.0 28.642344 7 GCAGAGT 9130 0.0 26.908432 9 GTACATG 4335 0.0 25.216955 1 TACATGG 4315 0.0 24.343708 2 GAGTACT 5500 0.0 24.276772 12-13 ACATGGG 4370 0.0 23.493492 3 AGAGTAC 7905 0.0 23.293127 10-11 AGTACTT 5865 0.0 22.441872 12-13 CAGGACG 170 3.1293985E-6 22.368538 4 GTACTTT 6060 0.0 21.953886 14-15 CAGAGTA 8990 0.0 21.221878 10-11 ATGGGTA 375 0.0 20.28081 5 >>END_MODULE