FastQCFastQC Report
Thu 26 May 2016
SRR937068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937068_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019384
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53410.5239438719854344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43170.42349104949655875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28450.2790901171688No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16420.16107767043626345No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11110.10898738846205158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67400.030.1005021
GGTATCA46250.028.4559961
TATCAAC81000.025.0404452
ATCAACG81100.024.9497783
TCAACGC81650.024.7817124
CAACGCA82800.024.4375235
AACGCAG84450.024.017486
GTACATG43900.022.8361931
GAGTACT47000.021.98220612-13
ACGCAGA92100.021.7130937
TACATGG45800.021.572332
CGCAGAG92650.021.431448
GTAAGGT3151.4551915E-1121.110354
GTACTTT48400.020.9522414-15
AGTACTT48400.020.80656412-13
TAATACC3201.8189894E-1120.7804994
ACATGGG43300.020.622813
AGAGTAC72550.020.52677210-11
GCAGAGT95400.020.0667579
GTATAGG4200.019.2311551
Produced by FastQC (version 0.11.5)