FastQCFastQC Report
Thu 26 May 2016
SRR937067_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937067_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062101
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96190.9056577481802578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67040.6312017407007432No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49770.46859950230721936No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT47190.444308027202686No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34110.3211558976029587No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26660.25101190941351154No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22400.21090272958974712No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC20300.19113059869070834No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19780.18623464246808918No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16450.15488169204247054No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15530.14621961564860592No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT14870.14000551736605088No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13510.12720070878381623No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13500.12710655577953509No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13280.12503518968535007No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13120.12352874161685187No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC13010.12249305856975937No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12710.11966846844132525No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC12260.1154315832486741No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA856.389638E-427.9540352
GTACATG56650.026.6759951
ACATGGG58750.024.4271053
TACATGG61950.023.9335272
GAGTACT58300.023.87700512-13
GTATATG2452.9467628E-1023.2760121
GATTGGC3750.022.8126459
CATGGGA36150.022.6106784
AGTACTT63850.022.210812-13
CATGGGG18250.021.8730754
CATATAG3059.094947E-1221.8122833
GTACTTT64000.021.6401314-15
TGGGAGT4450.021.3591466
GGATATG4900.021.3363461
TAGGCAT3451.8189894E-1220.6626515
ATGGGTA4150.020.6128645
GTATAGG5900.020.1363811
CATTCCG2851.9499566E-920.0110919
GTATCAA145950.019.8618261
ACTTTTT72550.019.845316-17
Produced by FastQC (version 0.11.5)