##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937067_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1062101 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9101055361025 33.0 31.0 34.0 30.0 34.0 2 32.01042556216405 33.0 31.0 34.0 30.0 34.0 3 32.00751623433176 33.0 31.0 34.0 30.0 34.0 4 35.59009077291143 37.0 35.0 37.0 33.0 37.0 5 35.61847037146185 37.0 35.0 37.0 33.0 37.0 6 35.69751370161595 37.0 35.0 37.0 33.0 37.0 7 35.64606190936644 37.0 35.0 37.0 33.0 37.0 8 35.6209654260753 37.0 35.0 37.0 33.0 37.0 9 37.242805533560364 39.0 37.0 39.0 34.0 39.0 10-11 37.12723648692544 39.0 37.0 39.0 33.5 39.0 12-13 37.04555357729632 39.0 37.0 39.0 33.0 39.0 14-15 38.17756974148409 40.0 38.0 41.0 33.0 41.0 16-17 38.12978709181142 40.0 38.0 41.0 33.0 41.0 18-19 38.14743936781907 40.0 38.0 41.0 33.0 41.0 20-21 38.11518396084742 40.0 38.0 41.0 33.0 41.0 22-23 37.98159826607827 40.0 38.0 41.0 33.0 41.0 24-25 37.820944053343325 40.0 37.5 41.0 33.0 41.0 26-27 37.77035140725788 40.0 37.0 41.0 33.0 41.0 28-29 37.83220616495041 40.0 37.0 41.0 33.0 41.0 30-31 37.676412601061486 40.0 37.0 41.0 33.0 41.0 32-33 37.45262503283586 40.0 36.5 41.0 33.0 41.0 34-35 37.25206971841661 40.0 36.0 41.0 32.0 41.0 36-37 37.07773460339459 39.5 36.0 41.0 31.0 41.0 38-39 36.90298709821383 39.0 35.5 41.0 31.0 41.0 40-41 36.72253768709379 39.0 35.0 41.0 31.0 41.0 42-43 36.500988135779934 39.0 35.0 41.0 30.0 41.0 44-45 36.216547672961426 39.0 35.0 41.0 30.0 41.0 46-47 35.80658195407028 39.0 35.0 40.5 28.5 41.0 48-49 35.419651238441546 39.0 35.0 40.0 27.0 41.0 50-51 34.75014899712927 38.5 34.0 39.5 26.0 40.5 52-53 35.04812724966834 38.5 35.0 40.0 27.0 40.5 54-55 35.37372199065814 39.0 35.0 40.5 27.0 41.0 56-57 35.25734087436129 39.0 35.0 41.0 26.5 41.0 58-59 35.039767875183244 38.5 35.0 40.5 26.0 41.0 60-61 34.76858603842761 38.0 34.5 40.0 26.0 41.0 62-63 34.47800491666988 38.0 34.0 40.0 25.5 41.0 64-65 34.176248304069006 37.5 34.0 40.0 24.5 41.0 66-67 33.79391225504919 37.0 34.0 39.5 23.0 41.0 68-69 33.35260347179788 36.0 33.0 39.0 22.0 41.0 70-71 32.98228087535931 36.0 33.0 39.0 22.0 40.5 72-73 32.607066559583316 35.0 33.0 38.5 21.0 40.0 74-75 32.18146155591606 35.0 32.0 37.5 19.5 39.5 76-77 31.753208499003392 35.0 32.0 37.0 18.5 39.0 78-79 31.314986051232417 35.0 31.5 36.5 16.0 39.0 80-81 30.87412025786625 35.0 31.0 36.0 11.0 38.0 82-83 30.465061232406335 35.0 31.0 35.5 7.0 37.0 84-85 30.102859803352036 34.0 31.0 35.0 4.5 37.0 86-87 29.81397249414133 34.0 30.5 35.0 2.0 36.0 88-89 29.42713781457696 34.0 30.0 35.0 2.0 36.0 90-91 29.078673308847275 34.0 29.5 35.0 2.0 36.0 92-93 28.748982441406234 34.0 29.0 35.0 2.0 35.0 94-95 28.367356776803714 34.0 29.0 35.0 2.0 35.0 96-97 27.955765977058682 34.0 29.0 35.0 2.0 35.0 98-99 27.426360581526616 34.0 28.0 35.0 2.0 35.0 100-101 25.356312629401536 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6337802303930751 1101 2 0.4834358652958386 1101 3 0.6163960590700341 1101 4 0.4163556212404629 1101 5 0.28134395583246885 1101 6 0.24658895911876755 1101 7 0.38562711713673536 1101 8 0.3868481237600676 1101 9 0.5148553795750175 1101 10-11 0.48725867880293094 1101 12-13 0.5971334855098007 1101 14-15 0.7950817438585389 1101 16-17 0.7656994888640725 1101 18-19 0.7001337819527791 1101 20-21 0.7594170057398628 1101 22-23 0.7976778386503796 1101 24-25 0.842679820978482 1101 26-27 0.8993126841543742 1101 28-29 0.7723047614103109 1101 30-31 0.7041131249141372 1101 32-33 0.7083534206300115 1101 34-35 0.744961109665816 1101 36-37 0.7841677405105685 1101 38-39 0.8669838142472273 1101 40-41 0.7706157639284612 1101 42-43 0.4965751548850008 1101 44-45 0.546151962999005 1101 46-47 0.7026870273505992 1101 48-49 0.7157459527236014 1101 50-51 0.853984797762088 1101 52-53 0.7250657136552547 1101 54-55 0.734483436154882 1101 56-57 0.7590311724174654 1101 58-59 0.939728537079084 1101 60-61 0.8563449872195363 1101 62-63 0.7794024427695234 1101 64-65 0.6928624166266886 1101 66-67 0.5763431930503202 1101 68-69 0.5141706783467725 1101 70-71 0.5653458535239864 1101 72-73 0.5811941118057433 1101 74-75 0.5521074773880486 1101 76-77 0.504046749573174 1101 78-79 0.830573149192606 1101 80-81 0.8456860960773547 1101 82-83 0.6243204048883406 1101 84-85 0.6866221280854141 1101 86-87 0.8182685086517374 1101 88-89 0.8610748195831768 1101 90-91 0.6114896436905894 1101 92-93 0.6264677305205169 1101 94-95 0.7806024776820522 1101 96-97 0.6476561327457482 1101 98-99 0.6215654058896654 1101 100-101 0.9307756741460373 1102 1 0.5168412800618931 1102 2 0.45830316525147907 1102 3 0.3775237417524906 1102 4 0.4122377132356263 1102 5 0.27172224656411714 1102 6 0.2463465042826769 1102 7 0.29007620162115444 1102 8 0.20714625907586992 1102 9 0.2619428621748625 1102 10-11 0.4486613220960294 1102 12-13 0.39780960562055157 1102 14-15 0.5941910856357211 1102 16-17 0.27765931937513955 1102 18-19 0.3102067478561281 1102 20-21 0.4081443090608019 1102 22-23 0.41604321885321127 1102 24-25 0.5261179376321508 1102 26-27 0.5844208327784273 1102 28-29 0.5186296967811828 1102 30-31 0.42847792383724226 1102 32-33 0.42437150148908387 1102 34-35 0.32799118324359 1102 36-37 0.3617223119697357 1102 38-39 0.2186546110593639 1102 40-41 0.25749681573883976 1102 42-43 0.1917130558532918 1102 44-45 0.24065810467829607 1102 46-47 0.289971915607218 1102 48-49 0.08121564204488152 1102 50-51 0.03945050990624566 1102 52-53 -0.02926367309462563 1102 54-55 0.11660755467476491 1102 56-57 0.2585485572705366 1102 58-59 0.2067397649560334 1102 60-61 0.25380341807019846 1102 62-63 0.16116134935175097 1102 64-65 0.11879705325038259 1102 66-67 0.05242697381608252 1102 68-69 0.12101409994284751 1102 70-71 0.21455921377975784 1102 72-73 0.21439627477822398 1102 74-75 0.06165729153149613 1102 76-77 0.3232783130626835 1102 78-79 0.35737626362823605 1102 80-81 0.316625551204087 1102 82-83 0.31512165311008644 1102 84-85 0.4585004264654806 1102 86-87 0.4736510824972058 1102 88-89 0.48846571490693336 1102 90-91 0.17385713485666443 1102 92-93 0.23939126164192714 1102 94-95 0.40287543440730644 1102 96-97 0.10163789891832664 1102 98-99 0.2655526024386994 1102 100-101 0.6401040666016371 1103 1 0.27337235580207064 1103 2 0.2972498191456978 1103 3 0.2806868251561312 1103 4 0.3100750675631403 1103 5 0.30524674364907867 1103 6 0.1718386346377443 1103 7 0.20563690985747485 1103 8 0.27466629003424714 1103 9 0.2631117312424962 1103 10-11 0.4141035574431413 1103 12-13 0.15907166701710906 1103 14-15 0.43760624509040014 1103 16-17 0.4667797969461773 1103 18-19 0.4769157073882013 1103 20-21 0.24611716972906805 1103 22-23 0.38022697712411 1103 24-25 0.48172946074625855 1103 26-27 0.5503055572298337 1103 28-29 0.6306270196428017 1103 30-31 0.6592383707036547 1103 32-33 0.7103177937793035 1103 34-35 0.6354299167414439 1103 36-37 0.7125091178645135 1103 38-39 0.8586802088161463 1103 40-41 0.8054148965731969 1103 42-43 0.7080629489580801 1103 44-45 0.8916721818585458 1103 46-47 0.6478734387847638 1103 48-49 0.6066865202350868 1103 50-51 0.7007116197133314 1103 52-53 0.7853484590367472 1103 54-55 0.8761837167241069 1103 56-57 0.7137810305784527 1103 58-59 0.8480309767100707 1103 60-61 1.042155645741147 1103 62-63 1.0795427273801863 1103 64-65 1.0153188351333782 1103 66-67 0.8776858061523569 1103 68-69 1.0525289986620052 1103 70-71 1.0045695861049992 1103 72-73 0.943233717920755 1103 74-75 0.7928078900812778 1103 76-77 0.7964585567964626 1103 78-79 1.049368955421091 1103 80-81 1.1287920983934079 1103 82-83 1.1537860021277275 1103 84-85 1.2102703828826797 1103 86-87 1.272433523768278 1103 88-89 1.3137118593835915 1103 90-91 1.414182641895259 1103 92-93 1.0987574396145305 1103 94-95 0.9906341874444635 1103 96-97 0.875827303286755 1103 98-99 0.9282640931987665 1103 100-101 1.4360777329489949 1104 1 0.4377505681179237 1104 2 0.3729681140338599 1104 3 0.40865933129720844 1104 4 0.3646333964135451 1104 5 0.24807157237957966 1104 6 0.36066447445782046 1104 7 0.4692995818561272 1104 8 0.4402597542475064 1104 9 0.5890212257625222 1104 10-11 0.5362096375914405 1104 12-13 0.614170539911477 1104 14-15 0.724834271715757 1104 16-17 0.6591938705353471 1104 18-19 0.6938875913203049 1104 20-21 0.5731584688495559 1104 22-23 0.6648106156207021 1104 24-25 0.4969978855853583 1104 26-27 0.6518042058697304 1104 28-29 0.6768908492093928 1104 30-31 0.8077705084451665 1104 32-33 0.7943103337529891 1104 34-35 0.9353759595571987 1104 36-37 0.9411765860573169 1104 38-39 0.9242665435516173 1104 40-41 0.8015771310811672 1104 42-43 0.821332527476315 1104 44-45 0.8129862418368958 1104 46-47 0.9153657745961326 1104 48-49 0.7438204053467885 1104 50-51 0.7253950060856553 1104 52-53 0.8735376688961694 1104 54-55 0.8845592328010738 1104 56-57 1.0748890810551615 1104 58-59 0.9912143857671012 1104 60-61 0.9380385343462976 1104 62-63 0.8950083470938353 1104 64-65 0.9290861808287971 1104 66-67 0.9299400174052721 1104 68-69 0.9007464671788767 1104 70-71 0.7128402664516145 1104 72-73 0.730852245489146 1104 74-75 0.7456245199123046 1104 76-77 0.8046234292112224 1104 78-79 0.9031101488956743 1104 80-81 1.0333867227671512 1104 82-83 0.9768509970398718 1104 84-85 0.988851487173978 1104 86-87 0.9292390964273771 1104 88-89 1.0603059133991088 1104 90-91 1.0003885606580987 1104 92-93 0.8119952595498461 1104 94-95 0.5752740515955033 1104 96-97 0.750039820057232 1104 98-99 0.851756694745216 1104 100-101 1.036571773353689 1105 1 0.44328204873778887 1105 2 0.45069751553020865 1105 3 0.5255081448519263 1105 4 0.4119850780176151 1105 5 0.39181705125382393 1105 6 0.3349258965620123 1105 7 0.2777677140750754 1105 8 0.42288759385409946 1105 9 0.4949232264349561 1105 10-11 0.5636755826892568 1105 12-13 0.44234653523837864 1105 14-15 0.7921345186159172 1105 16-17 0.8204070265443235 1105 18-19 0.7668415802692294 1105 20-21 0.7739393288228555 1105 22-23 0.8639604741710158 1105 24-25 0.7665817891890114 1105 26-27 0.5974967829029154 1105 28-29 0.6997359066213278 1105 30-31 0.5643424847672023 1105 32-33 0.7399901752206759 1105 34-35 0.765619786204077 1105 36-37 0.7429326507336143 1105 38-39 0.7252767286078736 1105 40-41 0.7852887673126574 1105 42-43 0.8381409268364806 1105 44-45 0.8680934583177375 1105 46-47 0.8952796651925254 1105 48-49 0.9569000009331532 1105 50-51 0.7945198350045573 1105 52-53 1.047640066239019 1105 54-55 1.140182409492617 1105 56-57 1.1003432313212471 1105 58-59 1.2384367589293603 1105 60-61 0.9934021602127459 1105 62-63 0.9648251417387854 1105 64-65 0.9545106147753657 1105 66-67 1.1268675197740805 1105 68-69 1.2176391759827183 1105 70-71 1.1506121707752541 1105 72-73 1.1288322998954854 1105 74-75 1.2644701852352256 1105 76-77 1.0388355731672583 1105 78-79 1.0867956347659948 1105 80-81 1.166395270878727 1105 82-83 1.3067738874358987 1105 84-85 1.3080378713261815 1105 86-87 1.419935765822288 1105 88-89 1.4991989720064858 1105 90-91 1.2896646201722461 1105 92-93 1.2086104858523576 1105 94-95 1.32166733944306 1105 96-97 1.2315032044573542 1105 98-99 1.3459059759499006 1105 100-101 1.4423833515110047 1106 1 0.35673748147532436 1106 2 0.37293802979800006 1106 3 0.41509735777133017 1106 4 0.33705367318851387 1106 5 0.3208867047502224 1106 6 0.33325021453002535 1106 7 0.4523884807730951 1106 8 0.32319823198517383 1106 9 0.3982834098780472 1106 10-11 0.46726409005834313 1106 12-13 0.5726881391894594 1106 14-15 0.8154348282741211 1106 16-17 0.6478558741454563 1106 18-19 0.6563161412601346 1106 20-21 0.8217614321467863 1106 22-23 0.8927400680876119 1106 24-25 0.8855978404590061 1106 26-27 0.8563676083968019 1106 28-29 0.7997398263453803 1106 30-31 0.7792619344379474 1106 32-33 0.8955287453053344 1106 34-35 0.8146648434320483 1106 36-37 0.8300416582594821 1106 38-39 1.0882162276671394 1106 40-41 0.9063097574349612 1106 42-43 0.8149057825906354 1106 44-45 0.9088007725228167 1106 46-47 0.7087679256189929 1106 48-49 0.756587402940049 1106 50-51 0.9487629362902226 1106 52-53 0.8523452049972491 1106 54-55 0.8826300811765293 1106 56-57 0.9520777092139951 1106 58-59 1.0927656041786449 1106 60-61 0.9722405499900972 1106 62-63 1.0079332869253648 1106 64-65 0.9114906633799436 1106 66-67 0.9608872947819265 1106 68-69 0.9759739792125757 1106 70-71 0.9381881154287512 1106 72-73 0.8249914602905903 1106 74-75 0.9211077479508134 1106 76-77 1.1251371175385394 1106 78-79 1.0913077271146854 1106 80-81 1.1558389640427258 1106 82-83 1.1272627750182131 1106 84-85 1.3083347904230784 1106 86-87 1.3312961060543316 1106 88-89 1.4140307178515776 1106 90-91 1.443260477023923 1106 92-93 1.3307358582261415 1106 94-95 1.346410107552181 1106 96-97 1.2035408730054868 1106 98-99 1.2946511742879352 1106 100-101 1.321407438215303 1107 1 0.23804992200814823 1107 2 0.3428317817802409 1107 3 0.3908981823828128 1107 4 0.2836541330679907 1107 5 0.2808167317086685 1107 6 0.20970716029596304 1107 7 0.22176585946006355 1107 8 0.22187310512187253 1107 9 0.28274166392181144 1107 10-11 0.35994764540245683 1107 12-13 0.4194144712541572 1107 14-15 0.3366175312554134 1107 16-17 0.5174889616811313 1107 18-19 0.3670212237212809 1107 20-21 0.38769516589242414 1107 22-23 0.5361223627276317 1107 24-25 0.510400112105053 1107 26-27 0.6169899323739685 1107 28-29 0.45744280763304346 1107 30-31 0.5262915490906508 1107 32-33 0.534931694877173 1107 34-35 0.6183721261786346 1107 36-37 0.5527254118275167 1107 38-39 0.605617809493971 1107 40-41 0.7779186418307731 1107 42-43 0.9195551244872391 1107 44-45 0.9493008914571632 1107 46-47 1.0995916450925094 1107 48-49 0.7957118479130969 1107 50-51 0.7358367548756988 1107 52-53 0.6894809685557632 1107 54-55 0.7888295340785234 1107 56-57 0.8038200974157093 1107 58-59 0.9001677806772221 1107 60-61 0.848960142441733 1107 62-63 0.8094447442675801 1107 64-65 0.9418357712137535 1107 66-67 0.9696701008396502 1107 68-69 0.9903615664310976 1107 70-71 0.932515442577504 1107 72-73 0.9333897119556767 1107 74-75 0.9810273681537964 1107 76-77 1.0902170017757733 1107 78-79 1.2231796546440314 1107 80-81 1.2549137539253756 1107 82-83 1.083888904894522 1107 84-85 1.0421091719644657 1107 86-87 1.1585191872468457 1107 88-89 1.081671159494455 1107 90-91 0.9766656665259852 1107 92-93 1.1123718992981395 1107 94-95 1.088112950083108 1107 96-97 0.8628286544834474 1107 98-99 1.060436240098273 1107 100-101 1.322279127875941 1108 1 0.08853220624596858 1108 2 0.21246402368178963 1108 3 0.25882824462148335 1108 4 0.15851774046679878 1108 5 0.23790696468905992 1108 6 0.2258534255391993 1108 7 0.147428618387778 1108 8 0.10394173351422609 1108 9 0.049745565841348593 1108 10-11 0.1257312154406094 1108 12-13 0.016868185061014174 1108 14-15 0.33668387261663213 1108 16-17 0.34639371818214926 1108 18-19 0.43252982627542735 1108 20-21 0.33505940615290086 1108 22-23 0.3694698081487715 1108 24-25 0.3933530010094657 1108 26-27 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-1.129818402053921 2316 70-71 -1.4413509676847056 2316 72-73 -1.1733993158747538 2316 74-75 -1.2281652841512098 2316 76-77 -1.7427636080551316 2316 78-79 -2.0384704720937528 2316 80-81 -1.8132614647171685 2316 82-83 -2.005549724981787 2316 84-85 -1.7148045015734041 2316 86-87 -1.516342966065281 2316 88-89 -1.823594337117644 2316 90-91 -1.7472614556937565 2316 92-93 -2.2096788845179205 2316 94-95 -2.3306003915683107 2316 96-97 -2.2681087532038333 2316 98-99 -2.2793415148061733 2316 100-101 -2.381496310685314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2370.0 3 605.0 4 598.0 5 646.0 6 662.0 7 865.0 8 1089.0 9 1296.0 10 1654.0 11 2316.0 12 3363.0 13 4232.0 14 4933.0 15 6425.0 16 8687.0 17 11949.0 18 10067.0 19 5960.0 20 5382.0 21 5608.0 22 5989.0 23 6647.0 24 7657.0 25 8754.0 26 10083.0 27 12167.0 28 14547.0 29 17914.0 30 21962.0 31 27509.0 32 35525.0 33 47039.0 34 65571.0 35 97287.0 36 144300.0 37 214246.0 38 208097.0 39 38096.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.55531218351822 20.68103346758049 15.394050731293717 21.369603617607574 2 14.435575074452444 22.00682323670223 40.49732348173559 23.06027820710974 3 17.709459045662896 27.85036250488757 31.80187777060672 22.63830067884281 4 11.344492262440665 18.259885833879338 40.250958062098455 30.144663841581544 5 11.666273951686632 38.96558874258947 35.60259757396394 13.76553973175996 6 27.41439774711816 39.70336678789981 18.470728275344687 14.411507189637346 7 25.383765625382914 34.99067802521175 23.107092429877824 16.51846391952751 8 24.94684660911524 33.8276096053078 21.411297922871036 19.814245862705924 9 26.024325340829073 17.134537817581624 22.626827629047508 34.21430921254179 10-11 22.897387852202616 27.473547691443457 28.963416358299888 20.66564809805404 12-13 25.02731704880693 26.120752899800735 28.77527739938868 20.076652652003652 14-15 22.36035058835836 26.81899163203772 28.300006455379428 22.520651324224495 16-17 19.61815570108468 30.202524892643996 28.871743974302422 21.307575431968903 18-19 19.506846784958228 28.7566852919572 32.021930066345234 19.714537856739334 20-21 20.668730381790436 27.422835631261748 31.690636823040748 20.217797163907065 22-23 20.40405696407969 27.221269805824832 31.808838958086298 20.56583427200918 24-25 20.701564480595554 27.819944124683634 31.619201852524398 19.859289542196414 26-27 20.090360438481753 27.434077246305204 32.45279321806851 20.022769097144536 28-29 19.505671414553813 27.671387231909723 32.64223052242775 20.180710831108716 30-31 19.923841371514694 27.638705727204222 32.044790881688016 20.392662019593068 32-33 19.81658566361353 28.014747752300686 31.873692698327172 20.294973885758612 34-35 20.518684647533505 27.57244168730284 31.63683408892033 20.27203957624333 36-37 20.715967499525473 27.08842301295846 31.69328489630359 20.502324591212474 38-39 20.00704606127059 27.94060019631005 31.758284302920348 20.29406943949901 40-41 19.765704969524876 27.898528366430682 32.14502569388081 20.19074097016363 42-43 19.73168165070285 28.06593352759352 31.121009399291356 21.081375422412275 44-45 20.029606296542433 27.766900574934873 31.01643417921182 21.187058949310874 46-47 20.657821990692558 28.232245267398238 30.52242046243352 20.587512279475686 48-49 20.426638584540264 28.37272806052477 30.260029689689993 20.94060366524498 50-51 20.045346668926936 28.45063193384943 30.517083874856375 20.986937522367256 52-53 20.36424807901335 28.957166565884336 29.799688045708084 20.878897309394233 54-55 20.16998557392738 28.427181301775263 29.969165035118518 21.433668089178845 56-57 20.645424436414604 28.79763111390774 29.34369571852192 21.21324873115573 58-59 20.84776044038965 28.17554541176781 29.75112445472954 21.225569693113002 60-61 20.66878935890612 28.629450470776085 30.134426708241293 20.5673334620765 62-63 20.41910292360253 28.69885155794048 30.33246457898701 20.549580939469976 64-65 20.399294885470695 28.741423312201565 30.02562468710896 20.833657115218784 66-67 20.372474426299316 28.820698634499102 29.216528081609734 21.59029885759184 68-69 20.123091966193275 28.753377092667048 29.96903625217133 21.154494688968352 70-71 20.778308983317174 28.61573842775822 29.680258004851012 20.925694584073597 72-73 20.62691461240664 28.22815540837688 29.881467069843744 21.263462909372738 74-75 20.070107001706056 29.590400501786817 29.239865493256534 21.099627003250596 76-77 20.276232920291633 28.63221518042455 29.697181768646097 21.39437013063772 78-79 20.235426563445763 28.72015657420906 29.67589986598868 21.368516996356494 80-81 20.50344380997233 29.259033378465887 29.065002649084594 21.172520162477188 82-83 20.390197534027703 28.7835566676213 29.637734953197775 21.188510845153218 84-85 20.137303975116836 28.671602412005193 29.31907158243915 21.872022030438824 86-87 20.130890261992505 28.946174641884227 29.49977082663046 21.42316426949281 88-89 20.561383967485284 28.351808032748423 29.27610841128893 21.810699588477366 90-91 20.284130734619126 28.129027517127547 29.865947272028396 21.72089447622493 92-93 20.71943259471084 28.602333409496172 29.554635843487727 21.123598152305263 94-95 20.139909032745006 28.006635502482425 29.908759485178482 21.944695979594087 96-97 19.97360418337412 28.15946428083415 29.896914017111577 21.97001751868015 98-99 19.88281767359476 28.670832290513378 29.300483444261094 22.145866591630764 100-101 19.80782110056071 28.21479960391616 29.813274087284547 22.16410520823858 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1597.0 1 1139.5 2 773.5 3 847.0 4 712.0 5 747.0 6 1321.0 7 2765.5 8 4539.0 9 4901.5 10 3661.5 11 2629.0 12 2525.0 13 2569.0 14 2496.5 15 2354.5 16 2228.5 17 2261.5 18 2295.5 19 2357.0 20 2577.0 21 2896.0 22 3355.5 23 3885.0 24 4236.5 25 5088.5 26 7085.0 27 8905.5 28 9449.5 29 10853.0 30 14066.0 31 17181.5 32 21450.5 33 26846.0 34 31916.5 35 35790.5 36 41312.0 37 46258.0 38 45070.5 39 42219.0 40 39184.5 41 36666.5 42 34399.0 43 34025.5 44 35379.5 45 35404.0 46 33673.0 47 35237.5 48 46413.0 49 44900.5 50 31472.5 51 25678.0 52 23411.0 53 22665.0 54 21872.5 55 20105.0 56 18027.5 57 16098.5 58 15011.5 59 13983.0 60 12292.0 61 10198.0 62 8151.5 63 6389.5 64 4775.0 65 3585.5 66 2576.5 67 1973.0 68 1403.5 69 823.0 70 568.0 71 397.5 72 263.0 73 207.0 74 158.0 75 124.5 76 81.0 77 45.0 78 29.0 79 16.5 80 13.0 81 12.0 82 8.5 83 6.0 84 5.0 85 6.5 86 6.0 87 4.5 88 3.0 89 1.5 90 1.5 91 0.5 92 0.5 93 1.0 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05893978067999183 2 0.09613021737104098 3 0.06929661115091691 4 0.12861300384803329 5 0.10460398775634333 6 0.09980218453800532 7 0.109876555996087 8 0.09613021737104098 9 0.11599650127436091 10-11 0.10686365985909062 12-13 0.08949243056922082 14-15 0.09137549065484356 16-17 0.10780518990190198 18-19 0.0856321573936942 20-21 0.07028521769586885 22-23 0.07207412477721045 24-25 0.059363469199256946 26-27 0.054090900959513265 28-29 0.06416527241759494 30-31 0.047170655144849694 32-33 0.04853587370692618 34-35 0.04274546394363624 36-37 0.048630026711207315 38-39 0.048488797204785605 40-41 0.06228221233197219 42-43 0.060069616731365474 44-45 0.047311884651271396 46-47 0.03483661158402073 48-49 0.042651310939355105 50-51 0.026456994202999528 52-53 0.0383202727424228 54-55 0.045334671561367515 56-57 0.04754726716197424 58-59 0.05522073701088691 60-61 0.052349070380312224 62-63 0.05832778615216444 64-65 0.040438715338748384 66-67 0.06204682982126935 68-69 0.05056016329897063 70-71 0.024526857615236212 72-73 0.019018906864789693 74-75 0.027822212765076015 76-77 0.0399679503173427 78-79 0.058704398169288984 80-81 0.0389322672702502 82-83 0.05771579162433705 84-85 0.06218805932769106 86-87 0.06378866040047039 88-89 0.08662076393864614 90-91 0.06148191179558253 92-93 0.07329811383286523 94-95 0.07908852359615517 96-97 0.08925704805851797 98-99 0.12009215696059039 100-101 0.11434882369944102 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1062101.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.04509482465129 #Duplication Level Percentage of deduplicated Percentage of total 1 71.14459570575741 25.644136984755043 2 13.92516418286026 10.038677268400715 3 5.3968585878187145 5.835908386594776 4 2.6667418471438444 3.8449185101266243 5 1.5330044309560225 2.7628645040210205 6 0.9357679051894207 2.023790572585078 7 0.6722094538847627 1.696089745511233 8 0.47334689499365795 1.3649466972000524 9 0.3599857948997935 1.1678149901421473 >10 2.365050914882988 17.135683494034623 >50 0.31000809731997586 7.8127871846419685 >100 0.20071507231366 12.996966179387126 >500 0.01103407465300535 2.538901268355022 >1k 0.004991605200169087 3.5907872587652117 >5k 5.254321263335881E-4 1.545726955479431 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9619 0.9056577481802578 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6704 0.6312017407007432 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4977 0.46859950230721936 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4719 0.444308027202686 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3411 0.3211558976029587 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2666 0.25101190941351154 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2240 0.21090272958974712 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2030 0.19113059869070834 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1978 0.18623464246808918 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1645 0.15488169204247054 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1553 0.14621961564860592 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1487 0.14000551736605088 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1351 0.12720070878381623 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1350 0.12710655577953509 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1328 0.12503518968535007 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1312 0.12352874161685187 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1301 0.12249305856975937 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1271 0.11966846844132525 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1226 0.1154315832486741 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 9.415300428113711E-5 0.0 4 0.0 0.0 0.0 9.415300428113711E-5 0.0 5 0.0 0.0 0.0 9.415300428113711E-5 0.0 6 0.0 0.0 0.0 9.415300428113711E-5 0.0 7 0.0 0.0 0.0 9.415300428113711E-5 0.0 8 0.0 0.0 0.0 9.415300428113711E-5 0.0 9 0.0 0.0 0.0 9.415300428113711E-5 0.0 10-11 0.0 0.0 0.0 9.415300428113711E-5 0.0 12-13 0.0 0.0 0.0 9.415300428113711E-5 0.0 14-15 0.0 0.0 0.0 1.8830600856227422E-4 0.0 16-17 0.0 0.0 0.0 1.8830600856227422E-4 0.0 18-19 0.0 0.0 0.0 2.3538251070284277E-4 0.0 20-21 0.0 0.0 0.0 3.295355149839799E-4 0.0 22-23 0.0 0.0 0.0 6.119945278273912E-4 0.0 24-25 0.0 0.0 0.0 0.001271065557795351 0.0 26-27 0.0 0.0 0.0 0.0017418305792010365 0.0 28-29 0.0 0.0 0.0 0.0032953551498397984 0.0 30-31 0.0 0.0 0.0 0.0073439343339286945 0.0 32-33 0.0 0.0 0.0 0.010921748496611905 0.0 34-35 0.0 0.0 0.0 0.014782021672138525 0.0 36-37 0.0 0.0 0.0 0.01793614731555662 0.0 38-39 0.0 0.0 0.0 0.02141980847395869 0.0 40-41 0.0 0.0 0.0 0.027068988730826915 0.0 42-43 0.0 0.0 0.0 0.03587229463111324 0.0 44-45 0.0 0.0 0.0 0.04881833271976959 0.0 46-47 0.0 0.0 0.0 0.08031251265180994 0.0 48-49 0.0 0.0 0.0 0.1420768834602359 0.0 50-51 0.0 0.0 0.0 0.18647002497879203 0.0 52-53 0.0 0.0 0.0 0.2287447239010226 0.0 54-55 0.0 0.0 0.0 0.278457510161463 0.0 56-57 0.0 0.0 0.0 0.3202143675601473 0.0 58-59 0.0 0.0 0.0 0.37567048708173706 0.0 60-61 0.0 0.0 0.0 0.46817581378795425 0.0 62-63 0.0 0.0 0.0 0.530834638137051 0.0 64-65 0.0 0.0 0.0 0.6058275060469767 0.0 66-67 0.0 0.0 0.0 0.7388657010962234 0.0 68-69 0.0 0.0 0.0 0.8780709179258845 0.0 70-71 0.0 0.0 0.0 1.0020233480620016 0.0 72-73 0.0 0.0 0.0 1.1429703954708639 0.0 74-75 0.0 0.0 0.0 1.2659342190620289 0.0 76-77 0.0 0.0 0.0 1.3626293544587567 0.0 78-79 0.0 0.0 0.0 1.4862522490798897 0.0 80-81 0.0 0.0 0.0 1.6680617003467655 0.0 82-83 0.0 0.0 0.0 1.866912845388527 0.0 84-85 0.0 0.0 0.0 2.1054965582369283 0.0 86-87 0.0 0.0 0.0 2.5125199957442845 0.0 88-89 0.0 0.0 0.0 3.0007974759462615 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTATA 85 6.389638E-4 27.954035 2 GTACATG 5665 0.0 26.675995 1 ACATGGG 5875 0.0 24.427105 3 TACATGG 6195 0.0 23.933527 2 GAGTACT 5830 0.0 23.877005 12-13 GTATATG 245 2.9467628E-10 23.276012 1 GATTGGC 375 0.0 22.812645 9 CATGGGA 3615 0.0 22.610678 4 AGTACTT 6385 0.0 22.2108 12-13 CATGGGG 1825 0.0 21.873075 4 CATATAG 305 9.094947E-12 21.812283 3 GTACTTT 6400 0.0 21.64013 14-15 TGGGAGT 445 0.0 21.359146 6 GGATATG 490 0.0 21.336346 1 TAGGCAT 345 1.8189894E-12 20.662651 5 ATGGGTA 415 0.0 20.612864 5 GTATAGG 590 0.0 20.136381 1 CATTCCG 285 1.9499566E-9 20.011091 9 GTATCAA 14595 0.0 19.861826 1 ACTTTTT 7255 0.0 19.8453 16-17 >>END_MODULE