FastQCFastQC Report
Thu 26 May 2016
SRR937067_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937067_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062101
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93840.8835317921741905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73220.6893882973464859No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61570.5797000473589611No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55290.5205719606704071No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28760.2707840403125503No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27180.25590786563613066No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25080.23613573473709187No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25000.23538251070284277No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18710.17616027101000753No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC17200.1619431673635558No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA16880.15893027122655942No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC16700.15723551714949896No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15270.14377163753729635No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14890.14019382337461314No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14580.13727508024189788No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13080.12315212959972734No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG12980.12221059955691597No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12650.11910355041563844No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA12420.11693803131717227No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTGC753.0797962E-431.664039
TATAGGG4050.028.1458032
TACGCTT350.008383064527.14954626-27
GTATATG2207.6397555E-1125.9130331
ATTCCGT1109.481329E-525.9069356
GTACATG58450.025.3588641
CTGGTCG5900.024.1505329
CATGGGT6650.023.568364
GTATAGG6700.023.3990821
TACATGG62250.023.3474522
GAGTACT68300.023.26069312-13
ACATGGG59500.022.98863
AGTACTT73000.022.05586412-13
GTACTTT73150.021.62106714-15
ATAACCC7300.021.4698053
CCCTGGT7100.021.406677
AATAACC6750.021.1093542
CATGGGG19300.020.9169644
GTGGTAC3900.020.708281
GATTGGC4850.020.56539
Produced by FastQC (version 0.11.5)