##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937067_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1062101 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.141530796035404 33.0 31.0 34.0 31.0 34.0 2 32.133061733300316 33.0 31.0 34.0 30.0 34.0 3 32.29934441263119 33.0 31.0 34.0 30.0 34.0 4 36.03910550879813 37.0 35.0 37.0 35.0 37.0 5 35.980739119914205 37.0 35.0 37.0 35.0 37.0 6 36.03690232849795 37.0 35.0 37.0 35.0 37.0 7 35.97541570905215 37.0 35.0 37.0 35.0 37.0 8 35.96003016662257 37.0 35.0 37.0 35.0 37.0 9 37.62558080634516 39.0 37.0 39.0 35.0 39.0 10-11 37.55764329381104 39.0 37.0 39.0 35.0 39.0 12-13 37.479840900253365 39.0 37.0 39.0 35.0 39.0 14-15 38.62442790280774 40.0 38.0 41.0 34.0 41.0 16-17 38.56305756232223 40.0 38.0 41.0 34.0 41.0 18-19 38.67958461577571 40.0 38.0 41.0 34.5 41.0 20-21 38.76760449335798 40.0 38.0 41.0 35.0 41.0 22-23 38.68522061461198 40.0 38.0 41.0 34.0 41.0 24-25 38.57503005834661 40.0 38.0 41.0 34.0 41.0 26-27 38.39215197048115 40.0 38.0 41.0 34.0 41.0 28-29 38.23291287740055 40.0 38.0 41.0 34.0 41.0 30-31 38.05174460809283 40.0 37.0 41.0 33.5 41.0 32-33 37.964370149354906 40.0 37.0 41.0 33.0 41.0 34-35 37.85058436062107 40.0 37.0 41.0 33.0 41.0 36-37 37.66238427418861 40.0 36.5 41.0 33.0 41.0 38-39 37.48585868952199 40.0 36.0 41.0 33.0 41.0 40-41 37.315153172815016 40.0 36.0 41.0 33.0 41.0 42-43 37.051709300716226 40.0 36.0 41.0 32.0 41.0 44-45 36.66069469852678 39.0 35.5 41.0 31.0 41.0 46-47 36.29418012034637 39.0 35.0 41.0 30.0 41.0 48-49 35.89213313988029 39.0 35.0 41.0 29.0 41.0 50-51 35.62513122574971 39.0 35.0 40.5 28.0 41.0 52-53 35.39340185161298 39.0 35.0 40.0 27.5 41.0 54-55 35.168859176293026 38.5 35.0 40.0 27.0 41.0 56-57 34.90656679543659 38.0 34.5 40.0 26.0 41.0 58-59 34.61315213901503 38.0 34.0 40.0 26.0 41.0 60-61 34.279420224630236 38.0 34.0 40.0 25.5 41.0 62-63 33.941015496642976 37.0 33.5 40.0 24.5 41.0 64-65 33.54523063249164 36.5 33.0 39.5 23.0 41.0 66-67 33.158395952927265 36.0 33.0 39.0 22.0 40.0 68-69 32.71735173961798 35.5 32.0 39.0 21.5 40.0 70-71 32.22869435204373 35.0 32.0 38.0 20.0 40.0 72-73 31.89903125973895 35.0 31.5 37.5 19.5 39.5 74-75 31.461478239828416 35.0 31.0 37.0 18.0 39.0 76-77 30.034443522791147 33.5 29.5 35.5 15.5 38.0 78-79 30.745456411395903 34.0 31.0 36.0 15.5 38.0 80-81 30.561905600314848 34.0 31.0 36.0 11.0 37.0 82-83 30.226498233218877 34.0 30.5 35.0 7.0 37.0 84-85 29.83767598373413 34.0 30.0 35.0 4.5 36.5 86-87 29.50629601139628 34.0 30.0 35.0 2.0 36.0 88-89 29.141369794398084 34.0 29.5 35.0 2.0 36.0 90-91 28.715259189097836 34.0 29.0 35.0 2.0 35.0 92-93 28.28656549612513 34.0 29.0 35.0 2.0 35.0 94-95 27.835013807538076 33.5 28.5 35.0 2.0 35.0 96-97 27.226712431303614 33.0 27.0 35.0 2.0 35.0 98-99 26.561281365896463 33.0 25.5 35.0 2.0 35.0 100-101 23.623745293526696 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3980349610384053 1101 2 0.24876173245629474 1101 3 0.24196714653155738 1101 4 0.20717459449132747 1101 5 0.27945344574779085 1101 6 0.25030470014651485 1101 7 0.2787749485164355 1101 8 0.2548920791288225 1101 9 0.30076540688185815 1101 10-11 0.38822359027522424 1101 12-13 0.3506046287853195 1101 14-15 0.4637815793754072 1101 16-17 0.5672828243991148 1101 18-19 0.45958000645287456 1101 20-21 0.5480415669760816 1101 22-23 0.4177579821653623 1101 24-25 0.4056083818947229 1101 26-27 0.3834125355804616 1101 28-29 0.3958783682034195 1101 30-31 0.3733667443680062 1101 32-33 0.49610875224538376 1101 34-35 0.48921975843515497 1101 36-37 0.5642575889263455 1101 38-39 0.45569429037367826 1101 40-41 0.4195822201136039 1101 42-43 0.5568917428975908 1101 44-45 0.7057385247132402 1101 46-47 0.8820634990217044 1101 48-49 0.5728628964792861 1101 50-51 0.584139092649778 1101 52-53 0.600444508207751 1101 54-55 0.5798191645760724 1101 56-57 0.6070727422497271 1101 58-59 0.5563735736115305 1101 60-61 0.5710756768527219 1101 62-63 0.6041553582240482 1101 64-65 0.48118666443172486 1101 66-67 0.4863877062560604 1101 68-69 0.525470639470818 1101 70-71 0.29818165120590123 1101 72-73 0.28398466549302626 1101 74-75 0.405716283386667 1101 76-77 0.37345598862422236 1101 78-79 0.44798717383639897 1101 80-81 0.5955990479996771 1101 82-83 0.5336946582442224 1101 84-85 0.4366030396176406 1101 86-87 0.4367154731760152 1101 88-89 0.5532730998870292 1101 90-91 0.5374972163891769 1101 92-93 0.5821070755311979 1101 94-95 0.48846915427261095 1101 96-97 0.3320493535178528 1101 98-99 0.30416853180908987 1101 100-101 0.7540135363496319 1102 1 0.21031908445327474 1102 2 0.13215472344646173 1102 3 0.17343885503506584 1102 4 0.10543840370920066 1102 5 0.11184984793217723 1102 6 0.22190383128627644 1102 7 0.13412195722033715 1102 8 0.2858796428018948 1102 9 0.2978200012862757 1102 10-11 0.33118704613620054 1102 12-13 0.27645704492042 1102 14-15 0.3518381929620631 1102 16-17 0.3365721334410736 1102 18-19 0.399805711254551 1102 20-21 0.35546856283064443 1102 22-23 0.2985608365547918 1102 24-25 0.3896369563826667 1102 26-27 0.37688537604977057 1102 28-29 0.25157108538024175 1102 30-31 0.3048919917943138 1102 32-33 0.42388551023415744 1102 34-35 0.24097162847324682 1102 36-37 0.38470372112160334 1102 38-39 0.3396287893258432 1102 40-41 0.47547876761792196 1102 42-43 0.4275952443431379 1102 44-45 0.5389999629216788 1102 46-47 0.4842452101256782 1102 48-49 0.522122529661182 1102 50-51 0.5310001820903665 1102 52-53 0.4552301230640339 1102 54-55 0.3000404332955853 1102 56-57 0.4478453089056842 1102 58-59 0.18326388000713223 1102 60-61 0.16716198159626572 1102 62-63 0.4124779837728241 1102 64-65 0.4294302053431096 1102 66-67 0.4172880779691539 1102 68-69 0.07891548673192261 1102 70-71 0.2368134257917376 1102 72-73 0.419430491751573 1102 74-75 0.14343368965531056 1102 76-77 0.2507279510551932 1102 78-79 0.17573677994289838 1102 80-81 0.22378614358959936 1102 82-83 0.2587267815979004 1102 84-85 0.21528269329729355 1102 86-87 0.13441559402101433 1102 88-89 0.22376244564139824 1102 90-91 0.4429279752651638 1102 92-93 0.2683854320392385 1102 94-95 0.2856679213477058 1102 96-97 0.3757218176850152 1102 98-99 0.43345049837020966 1102 100-101 0.5057351951384277 1103 1 0.0679565415524479 1103 2 -0.22965386750328776 1103 3 -0.08554573555942113 1103 4 0.07827052429461645 1103 5 0.05430247463631588 1103 6 0.06916579117995525 1103 7 0.16759570286681225 1103 8 0.20107874703900563 1103 9 0.06613492664994425 1103 10-11 0.18992993300489758 1103 12-13 0.14178651691514688 1103 14-15 0.20845348437047306 1103 16-17 0.25798828889273295 1103 18-19 0.3320628497615701 1103 20-21 0.19335815942014278 1103 22-23 0.2522097063836952 1103 24-25 0.25467302162363836 1103 26-27 0.17810309337694719 1103 28-29 0.2598784614674301 1103 30-31 0.4032609674399694 1103 32-33 0.45495173451745075 1103 34-35 0.3928302413095537 1103 36-37 0.3324273605072534 1103 38-39 0.2998777251316156 1103 40-41 0.34174952019831295 1103 42-43 0.40661428247649667 1103 44-45 0.5267450765097408 1103 46-47 0.4950116520057648 1103 48-49 0.6215334957782517 1103 50-51 0.29197676664543337 1103 52-53 0.48563340668210486 1103 54-55 0.5206672452805279 1103 56-57 0.5709294421028375 1103 58-59 0.5211590275352265 1103 60-61 0.32603460182959765 1103 62-63 0.36008267447452624 1103 64-65 0.3199728333794667 1103 66-67 0.321683469738538 1103 68-69 0.47742529761936936 1103 70-71 0.36965452303783763 1103 72-73 0.3341490433055192 1103 74-75 0.18982526237894604 1103 76-77 0.353919899339477 1103 78-79 0.5290040623936854 1103 80-81 0.5503613413616115 1103 82-83 0.6479593881283421 1103 84-85 0.5278949015558219 1103 86-87 0.3531606378258516 1103 88-89 0.7011051003222661 1103 90-91 0.8685350893480575 1103 92-93 0.7724923336004785 1103 94-95 0.7378481714254157 1103 96-97 1.0485986629756034 1103 98-99 1.2059222837423746 1103 100-101 1.324788871128554 1104 1 0.20449252273062513 1104 2 0.05236558948976011 1104 3 0.18455939183138526 1104 4 0.1412205725806288 1104 5 0.049144945583691424 1104 6 0.1311695633218477 1104 7 0.14139001095099246 1104 8 0.24259661286223633 1104 9 0.2802542092948528 1104 10-11 0.15566307016957381 1104 12-13 0.2364173719987832 1104 14-15 0.25769792175745465 1104 16-17 0.2823694826265637 1104 18-19 0.33667027478863787 1104 20-21 0.32001009183269247 1104 22-23 0.2771888040510575 1104 24-25 0.3634939680294096 1104 26-27 0.25165256871219555 1104 28-29 0.231570383769089 1104 30-31 0.28573800728079135 1104 32-33 0.23749293965845197 1104 34-35 0.21019003364730793 1104 36-37 0.20301194527998945 1104 38-39 0.09034156247582104 1104 40-41 0.12743498708189094 1104 42-43 0.09922846754477632 1104 44-45 0.14922626441036613 1104 46-47 0.10637976638851399 1104 48-49 0.18712712202510318 1104 50-51 0.2605163255252023 1104 52-53 0.15573464526683978 1104 54-55 0.22050164695530583 1104 56-57 0.19016266676564442 1104 58-59 0.03153727873913681 1104 60-61 0.055229489345883565 1104 62-63 0.2504823465185453 1104 64-65 0.03570516037621019 1104 66-67 0.34086986669489505 1104 68-69 0.10426437882411932 1104 70-71 0.10743313978951363 1104 72-73 0.28795812885276817 1104 74-75 0.1648599375664226 1104 76-77 0.055428107960960205 1104 78-79 -0.09091213383547014 1104 80-81 0.0756871909037038 1104 82-83 0.15581477639322117 1104 84-85 0.141275473080686 1104 86-87 0.05686683204578813 1104 88-89 0.2543096385589507 1104 90-91 0.19202704276015936 1104 92-93 0.19642404616585907 1104 94-95 0.14078681254694558 1104 96-97 0.20298993519850583 1104 98-99 0.41167982316514795 1104 100-101 0.6292921085596106 1105 1 0.3124493644462234 1105 2 0.10859565832973317 1105 3 0.21911098106014748 1105 4 0.22160406592926307 1105 5 0.20428026521242515 1105 6 0.3165331722075351 1105 7 0.37379964786850906 1105 8 0.2899924444267441 1105 9 0.3465445258500637 1105 10-11 0.3959738519295257 1105 12-13 0.43551501473051246 1105 14-15 0.4394357151424302 1105 16-17 0.5375010664894191 1105 18-19 0.42462421279530105 1105 20-21 0.4615217525164752 1105 22-23 0.48386835344977186 1105 24-25 0.42177149128455227 1105 26-27 0.5150635437131257 1105 28-29 0.36725807034512314 1105 30-31 0.3483868011378277 1105 32-33 0.2801851023612443 1105 34-35 0.2864281004224267 1105 36-37 0.33281834770347984 1105 38-39 0.5041001335114075 1105 40-41 0.07019328982156736 1105 42-43 0.1453634781237909 1105 44-45 0.21656429348820438 1105 46-47 -0.2579376412616554 1105 48-49 -0.23969813922380467 1105 50-51 -0.28567557057975534 1105 52-53 -0.1526417908804376 1105 54-55 -0.1947227876704858 1105 56-57 -0.14502680448763527 1105 58-59 -0.14488766123390207 1105 60-61 -0.13063759889630688 1105 62-63 -0.12765569682313327 1105 64-65 -0.1955332290332379 1105 66-67 -0.12450892298488014 1105 68-69 -0.13680756523448423 1105 70-71 -0.2948337059675019 1105 72-73 -0.07415390336991123 1105 74-75 0.0401324786683368 1105 76-77 0.07833599416273884 1105 78-79 0.005607321014402089 1105 80-81 0.0800121889509171 1105 82-83 0.0919688393782323 1105 84-85 0.21304845058797994 1105 86-87 0.1156300048657144 1105 88-89 0.25315622084259104 1105 90-91 0.3336312485533135 1105 92-93 0.4527264530179309 1105 94-95 0.5695754734786469 1105 96-97 0.7654914210144845 1105 98-99 0.5952209726263504 1105 100-101 0.6098890499861973 1106 1 0.38868701731546196 1106 2 0.20012807444764036 1106 3 0.29024907293847946 1106 4 0.19099494081167023 1106 5 0.16505950635384892 1106 6 0.3201775356443477 1106 7 0.2933718099883009 1106 8 0.3455711916829358 1106 9 0.3566907315571868 1106 10-11 0.43246060326223557 1106 12-13 0.4343302781359739 1106 14-15 0.5453765469078746 1106 16-17 0.6587195127108032 1106 18-19 0.6197666947645644 1106 20-21 0.5372596405691539 1106 22-23 0.6227363371437136 1106 24-25 0.593967418691264 1106 26-27 0.7048208148012449 1106 28-29 0.6567076430952028 1106 30-31 0.5693759435022088 1106 32-33 0.5878633518124872 1106 34-35 0.5130969229373221 1106 36-37 0.4587448724761387 1106 38-39 0.6119307622351471 1106 40-41 0.5044374690313518 1106 42-43 0.47261143986727916 1106 44-45 0.7684482108604271 1106 46-47 0.5144498626580685 1106 48-49 0.41013968219357366 1106 50-51 0.24490072684891118 1106 52-53 0.2585005147012467 1106 54-55 0.5059352360046461 1106 56-57 0.38013145437093954 1106 58-59 0.39578780521325996 1106 60-61 0.23106620715574877 1106 62-63 0.1360682370119335 1106 64-65 0.1892156146481696 1106 66-67 0.19057466513051935 1106 68-69 0.07009996847514799 1106 70-71 0.1030991295608743 1106 72-73 0.24688374557959847 1106 74-75 0.35842191109229304 1106 76-77 0.4821748739056062 1106 78-79 0.49540329937752503 1106 80-81 0.2823752925884193 1106 82-83 0.3520185218805878 1106 84-85 0.20122019329729213 1106 86-87 0.17083343854398336 1106 88-89 0.44266703928096973 1106 90-91 0.7308751925796528 1106 92-93 0.7077902465268693 1106 94-95 0.6805957776492342 1106 96-97 0.7699687258122268 1106 98-99 1.1640508370038987 1106 100-101 1.1510035255271198 1107 1 0.21206653682069998 1107 2 0.07435599230365142 1107 3 0.21169789152395424 1107 4 0.16472234328550428 1107 5 0.11804872297294366 1107 6 0.08186977319588351 1107 7 0.07541966124107091 1107 8 0.12735738736958524 1107 9 0.2912193550903268 1107 10-11 0.2732689603824383 1107 12-13 0.28254328955367214 1107 14-15 0.35941426891865547 1107 16-17 0.27145242580944284 1107 18-19 0.37925864687366584 1107 20-21 0.247324459788949 1107 22-23 0.36743979416877437 1107 24-25 0.344371299915494 1107 26-27 0.3561950020831972 1107 28-29 0.3463806727494969 1107 30-31 0.2598146130232948 1107 32-33 0.2892375390944366 1107 34-35 0.36824777133567466 1107 36-37 0.3539602427584043 1107 38-39 0.3881329902719344 1107 40-41 0.3540375522853978 1107 42-43 0.3910323881704869 1107 44-45 0.5102808900548439 1107 46-47 0.4706602178753201 1107 48-49 0.1176003347466974 1107 50-51 0.10904548977310924 1107 52-53 0.21204426271901156 1107 54-55 0.06288744421905079 1107 56-57 0.06394498513528646 1107 58-59 0.035422181204381786 1107 60-61 0.01010055936000498 1107 62-63 -0.02558832023513702 1107 64-65 -0.02390928623987776 1107 66-67 -0.20903532931921376 1107 68-69 -0.02814475876874667 1107 70-71 0.0010212473851751724 1107 72-73 -0.1928586624445039 1107 74-75 0.07332042044846077 1107 76-77 0.04858830952247928 1107 78-79 0.08979851821672824 1107 80-81 0.09188458928997534 1107 82-83 0.1535631817283516 1107 84-85 0.27772322612317524 1107 86-87 0.19323472303494427 1107 88-89 0.1960923485102768 1107 90-91 0.49627570399735177 1107 92-93 0.570800177544946 1107 94-95 0.6313192949343609 1107 96-97 0.974086827620031 1107 98-99 1.1001023157542917 1107 100-101 0.9573679998373024 1108 1 0.14146798061821642 1108 2 0.07026377781155446 1108 3 0.11546887416172069 1108 4 0.12138484906855496 1108 5 0.09938672347923472 1108 6 0.0561389270816548 1108 7 0.15003274271001033 1108 8 0.17998441798263087 1108 9 0.3028107621382219 1108 10-11 0.2735347622836386 1108 12-13 0.225643351530465 1108 14-15 0.34125288482836424 1108 16-17 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-0.6027321722312777 2316 82-83 -0.379625882253098 2316 84-85 -0.3521095670369192 2316 86-87 -0.23599923515874366 2316 88-89 -0.2548263644129598 2316 90-91 -0.4248347348609798 2316 92-93 -0.7994038497791998 2316 94-95 -0.7152430526058247 2316 96-97 -0.5810564500892674 2316 98-99 -0.7163182812898583 2316 100-101 -0.6968265646223983 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 6.0 5 20.0 6 65.0 7 132.0 8 325.0 9 603.0 10 1064.0 11 1933.0 12 2943.0 13 3901.0 14 4780.0 15 6818.0 16 9668.0 17 13813.0 18 11830.0 19 6604.0 20 5297.0 21 5379.0 22 5706.0 23 6216.0 24 7331.0 25 8364.0 26 10272.0 27 12636.0 28 15956.0 29 19762.0 30 24523.0 31 30928.0 32 39083.0 33 51146.0 34 70632.0 35 101931.0 36 152317.0 37 233006.0 38 183135.0 39 13972.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.239333591981406 21.28422535529582 15.807415164059469 20.669025888663302 2 14.866247927410159 23.03580625510203 39.76397191540727 22.333973902080544 3 18.3264584006154 28.062059881119062 31.622367996113255 21.989113722152283 4 11.996339324300278 18.866602830809555 39.6039352263772 29.533122618512962 5 11.785940593872962 39.694975609159016 35.05483113779672 13.464252659171304 6 28.046617254937406 39.90863349452404 17.727116946114098 14.317632304424457 7 25.892264483321263 35.27442305392802 22.567816055158595 16.265496407592124 8 25.723071534628062 33.92426897253651 20.882006513504837 19.47065297933059 9 26.469139940551795 17.498712457666453 22.18376595069584 33.848381651085916 10-11 23.507296864702003 27.797147161284247 28.405658600885037 20.289897373128706 12-13 25.562414643894684 26.265082815135564 28.43150962555592 19.740992915413834 14-15 22.576995973076006 27.157398401846905 28.133011832208048 22.13259379286904 16-17 19.808756417704153 30.375171476159046 28.75103215230943 21.065039953827366 18-19 19.612494480280123 28.890802287164778 32.03221727500492 19.464485957550178 20-21 20.856228744458967 27.38528127930581 32.04078615842934 19.71770381780588 22-23 20.673316379515697 27.255082143788584 31.997568969429462 20.074032507266256 24-25 20.96347527897396 27.857238022468568 31.899518059924027 19.27976863863345 26-27 20.453148112055484 27.407043342268622 32.758989191973114 19.380819353702776 28-29 19.713634559696892 27.762825314162587 32.853829245637876 19.66971088050264 30-31 20.206590577477115 27.62309720134224 32.271832482622855 19.898479738557782 32-33 20.145251138908467 28.098332394148716 32.05025179092385 19.706164676018968 34-35 20.781214544708153 27.37546336094276 32.05271369213343 19.79060840221566 36-37 20.99312703290164 27.05215668237043 32.02518314651863 19.929533138209298 38-39 20.16711578622286 28.137434350396113 32.010831596172785 19.684618267208247 40-41 20.066832634060727 28.028559699300224 32.32607320141311 19.57853446522594 42-43 20.02830316588706 28.367521222666635 31.17312197289826 20.431053638548047 44-45 20.46335736129314 27.88128380108766 31.046016555942213 20.609342281676987 46-47 21.14325393089163 28.46210338009604 30.482581677808117 19.91206101120422 48-49 20.90026447628705 28.64784732873301 30.139986950369035 20.311901244610908 50-51 20.567621285774678 28.66404167596429 30.334272040553657 20.43406499770737 52-53 20.870792903987848 29.15225129332321 29.68987076889471 20.287085033794234 54-55 20.667365939272507 28.71199823738108 29.7553518835165 20.865283939829915 56-57 21.056192057516324 29.056109202677526 29.239472610120437 20.648226129685714 58-59 21.32027728412207 28.41611449150847 29.51357585886285 20.750032365506605 60-61 20.903011135580662 29.081877164951024 29.96974835489614 20.04536334457217 62-63 20.71422585987585 28.938584936837458 30.276122515655292 20.071066687631404 64-65 20.68922011978168 29.068476667429305 29.77570829084671 20.466594921942306 66-67 20.76093552352155 29.0858539281358 29.109015578092656 21.04419497024999 68-69 20.545174140689067 28.956850619667996 29.722408697477924 20.77556654216501 70-71 21.18089081359368 29.02132715812546 29.38584205860767 20.411939969673192 72-73 21.066405172389445 28.507270024225566 29.682487823662722 20.743836979722268 74-75 20.61721234763647 30.069729911929034 28.79809584426296 20.514961896171542 76-77 20.86093214059409 29.024153252116342 29.279968891613066 20.834945715676497 78-79 20.85775431480493 29.136031575210975 29.06904158651877 20.93717252346533 80-81 20.996091956945147 29.737896059519386 28.639727016060124 20.626284967475346 82-83 21.01645396387952 29.213730169303854 29.077629887264152 20.692185979552473 84-85 20.551112969119707 29.240002297402523 28.749262053595388 21.459622679882383 86-87 20.541167309826193 29.405470143572792 28.944155989057265 21.10920655754375 88-89 20.943340799764993 28.850461402879425 28.758896399259193 21.44730139809639 90-91 20.792062894266078 28.568684681291778 29.190377553902646 21.4488748705395 92-93 21.0221631568764 29.121161321362717 29.007141979501938 20.84953354225895 94-95 20.593362486283066 28.339717700648574 29.182529508770127 21.884390304298233 96-97 20.44922894698313 28.639286913071583 29.13839880273405 21.773085337211235 98-99 20.480685531461543 29.127695864618648 28.446900453031898 21.944718150887915 100-101 20.12873631182189 28.981864591017647 28.825663653906176 22.06373544325429 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2600.0 1 1677.0 2 875.0 3 942.0 4 809.0 5 858.0 6 1408.5 7 2832.0 8 4205.5 9 4511.0 10 3723.5 11 2882.0 12 2734.0 13 2697.5 14 2653.0 15 2524.0 16 2425.0 17 2451.5 18 2529.5 19 2554.5 20 2711.0 21 3153.5 22 3567.0 23 3947.0 24 4373.5 25 5267.5 26 7152.0 27 8838.0 28 9344.5 29 10972.0 30 14003.5 31 16834.5 32 21127.5 33 26474.0 34 31604.0 35 35114.5 36 40069.5 37 45350.0 38 45124.0 39 42351.5 40 39143.5 41 36405.5 42 33957.0 43 33753.5 44 35092.0 45 35235.0 46 33548.5 47 35354.5 48 47051.0 49 44930.5 50 31177.5 51 25762.0 52 23341.0 53 22465.0 54 21547.0 55 20009.0 56 18065.5 57 16097.0 58 15059.0 59 14176.5 60 12446.5 61 10492.5 62 8638.5 63 6680.0 64 4804.5 65 3609.0 66 2735.0 67 2097.0 68 1497.5 69 873.0 70 583.5 71 424.5 72 298.5 73 235.0 74 174.5 75 136.5 76 101.0 77 51.5 78 28.0 79 18.0 80 12.5 81 8.0 82 6.0 83 2.5 84 1.5 85 2.5 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015911857723512172 2 0.0022596721027472906 3 0.002824590128434113 4 1.8830600856227422E-4 5 0.0020713660941850164 6 4.7076502140568554E-4 7 0.0 8 0.0 9 0.0 10-11 9.415300428113711E-5 12-13 6.119945278273912E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0023538251070284277 22-23 0.0 24-25 0.0037190436691049156 26-27 0.0018830600856227422 28-29 0.0029658196348558187 30-31 0.013699262122905448 32-33 8.944535406708024E-4 34-35 0.0022596721027472906 36-37 0.00390734967766719 38-39 0.0021655190984661535 40-41 0.0042368851926511695 42-43 0.002730437124152976 44-45 4.7076502140568554E-4 46-47 9.415300428113711E-5 48-49 1.8830600856227422E-4 50-51 1.8830600856227422E-4 52-53 0.0010827595492330767 54-55 0.0042368851926511695 56-57 2.3538251070284277E-4 58-59 0.001271065557795351 60-61 9.415300428113711E-4 62-63 0.0 64-65 1.8830600856227422E-4 66-67 4.7076502140568554E-5 68-69 0.0 70-71 4.23688519265117E-4 72-73 0.0 74-75 2.824590128434113E-4 76-77 7.532240342490969E-4 78-79 1.8830600856227422E-4 80-81 0.005696256759008794 82-83 0.0015535245706387623 84-85 0.0030128961369963875 86-87 0.0024950546134501336 88-89 0.0013181420599359193 90-91 9.415300428113711E-5 92-93 4.7076502140568554E-5 94-95 1.4122950642170566E-4 96-97 0.001365218562076488 98-99 0.00324827864769923 100-101 7.061475321085282E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1062101.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.23202239862269 #Duplication Level Percentage of deduplicated Percentage of total 1 69.24863754128499 23.70520911387372 2 14.672787658732977 10.045583915679638 3 5.861583046808651 6.019615264492223 4 2.81536351192525 3.855023472019608 5 1.6121227395745281 2.7593110865222115 6 1.013456266055415 2.0815594559780086 7 0.7080359948608866 1.696625282457628 8 0.5317121230834733 1.4561265045610146 9 0.4048178773611975 1.2471961180672266 >10 2.5623532992954643 17.359776009507865 >50 0.33538599239581024 7.990572006273561 >100 0.21657318462518302 13.753527041006365 >500 0.01107791225570716 2.469760759628697 >1k 0.004985060515068222 2.8684150912949926 >5k 0.001107791225570716 2.6916988786372102 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9384 0.8835317921741905 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7322 0.6893882973464859 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6157 0.5797000473589611 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5529 0.5205719606704071 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2876 0.2707840403125503 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2718 0.25590786563613066 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2508 0.23613573473709187 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2500 0.23538251070284277 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1871 0.17616027101000753 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1720 0.1619431673635558 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1688 0.15893027122655942 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1670 0.15723551714949896 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1527 0.14377163753729635 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1489 0.14019382337461314 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1458 0.13727508024189788 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1308 0.12315212959972734 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1298 0.12221059955691597 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1265 0.11910355041563844 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1242 0.11693803131717227 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 9.415300428113711E-5 0.0 16-17 0.0 0.0 0.0 9.415300428113711E-5 0.0 18-19 0.0 0.0 0.0 1.4122950642170566E-4 0.0 20-21 0.0 0.0 0.0 2.3538251070284277E-4 0.0 22-23 0.0 0.0 0.0 5.178415235462541E-4 0.0 24-25 0.0 0.0 0.0 0.0011769125535142139 0.0 26-27 0.0 0.0 0.0 0.0016476775749198994 0.0 28-29 0.0 0.0 0.0 0.003342431651980367 0.0 30-31 0.0 0.0 0.0 0.0074851638403504 0.0 32-33 0.0 0.0 0.0 0.01101590150089304 0.0 34-35 0.0 0.0 0.0 0.014876174676419662 0.0 36-37 0.0 0.0 0.0 0.01793614731555662 0.0 38-39 0.0 0.0 0.0 0.02174934398894267 0.0 40-41 0.0 0.0 0.0 0.027445600747951465 0.0 42-43 0.0 0.0 0.0 0.036343059652518925 0.0 44-45 0.0 0.0 0.0 0.04914786823475357 0.0 46-47 0.0 0.0 0.0 0.0815835782096053 0.0 48-49 0.0 0.0 0.0 0.14391286704371808 0.0 50-51 0.0 0.0 0.0 0.1886355440772582 0.0 52-53 0.0 0.0 0.0 0.2308160899952076 0.0 54-55 0.0 0.0 0.0 0.28109379428133485 0.0 56-57 0.0 0.0 0.0 0.3230389576885814 0.0 58-59 0.0 0.0 0.0 0.3786833832187334 0.0 60-61 0.0 0.0 0.0 0.47128286292923177 0.0 62-63 0.0 0.0 0.0 0.5336592282654851 0.0 64-65 0.0 0.0 0.0 0.6087933256818325 0.0 66-67 0.0 0.0 0.0 0.7422552092503444 0.0 68-69 0.0 0.0 0.0 0.8824961091270981 0.0 70-71 0.0 0.0 0.0 1.007107610293183 0.0 72-73 0.0 0.0 0.0 1.147866351693483 0.0 74-75 0.0 0.0 0.0 1.271253863803913 0.0 76-77 0.0 0.0 0.0 1.3690788352520147 0.0 78-79 0.0 0.0 0.0 1.4932666478988343 0.0 80-81 0.0 0.0 0.0 1.67507609916571 0.0 82-83 0.0 0.0 0.0 1.8748687742502832 0.0 84-85 0.0 0.0 0.0 2.11387617561795 0.0 86-87 0.0 0.0 0.0 2.5200993125889157 0.0 88-89 0.0 0.0 0.0 3.0067291152159727 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTTGC 75 3.0797962E-4 31.66403 9 TATAGGG 405 0.0 28.145803 2 TACGCTT 35 0.0083830645 27.149546 26-27 GTATATG 220 7.6397555E-11 25.913033 1 ATTCCGT 110 9.481329E-5 25.906935 6 GTACATG 5845 0.0 25.358864 1 CTGGTCG 590 0.0 24.150532 9 CATGGGT 665 0.0 23.56836 4 GTATAGG 670 0.0 23.399082 1 TACATGG 6225 0.0 23.347452 2 GAGTACT 6830 0.0 23.260693 12-13 ACATGGG 5950 0.0 22.9886 3 AGTACTT 7300 0.0 22.055864 12-13 GTACTTT 7315 0.0 21.621067 14-15 ATAACCC 730 0.0 21.469805 3 CCCTGGT 710 0.0 21.40667 7 AATAACC 675 0.0 21.109354 2 CATGGGG 1930 0.0 20.916964 4 GTGGTAC 390 0.0 20.70828 1 GATTGGC 485 0.0 20.5653 9 >>END_MODULE