FastQCFastQC Report
Thu 26 May 2016
SRR937066_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937066_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969453
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94810.9779741771906426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66940.6904924735907775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49300.5085341940248779No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41960.432821395157888No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32510.3353437453904418No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25380.261797116518284No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20020.2065082061739971No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19830.2045483380834347No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18320.18897254431107027No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14800.15266340915959825No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT14060.14503023870161832No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT13540.13966638919060542No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13160.1357466530094806No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12870.13275527539756957No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12200.12584416160453368No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT11820.12192442542340887No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA11440.11800468924228404No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11320.1167668778166657No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC10820.1116093302099225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGGA1153.859732E-628.9169062
GTACATG52100.025.3488751
ACACCGA950.001224724325.005926
CCCTTAC950.001225461425.003341
GAGTACT54400.024.32582512-13
CAACGCT1000.001651677923.7543984
ACATGGG52700.023.5278113
TACATGG54300.023.2719862
CATGGGG15750.022.924884
GTATAGG4150.022.8946251
CTCCCGC2154.40632E-822.0993967
GTACTTT60100.021.93795814-15
GTATCAA125550.021.9085431
AGTACTT59900.021.8939212-13
CAACGCG1100.002873954821.5949084
AAACGGT550.002879287321.58933666-67
CATGGGA32700.021.3571644
TACACTG2901.05501385E-1021.2992445
GACAGTA1805.154481E-621.1171997
ATGGGAT14250.020.6726285
Produced by FastQC (version 0.11.5)