##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937066_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 969453 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.97921817767339 33.0 31.0 34.0 30.0 34.0 2 32.09914766368251 33.0 31.0 34.0 30.0 34.0 3 32.09731260824403 34.0 31.0 34.0 30.0 34.0 4 35.688532605500214 37.0 35.0 37.0 35.0 37.0 5 35.69846501068128 37.0 35.0 37.0 35.0 37.0 6 35.785675014673224 37.0 35.0 37.0 35.0 37.0 7 35.736459632390634 37.0 35.0 37.0 35.0 37.0 8 35.69012525620118 37.0 35.0 37.0 33.0 37.0 9 37.34793538211755 39.0 37.0 39.0 34.0 39.0 10-11 37.17783791478287 39.0 37.0 39.0 34.0 39.0 12-13 37.14361088160024 39.0 37.0 39.0 33.5 39.0 14-15 38.30864105841129 40.0 38.0 41.0 33.5 41.0 16-17 38.26242014826918 40.0 38.0 41.0 33.0 41.0 18-19 38.29818413063862 40.0 38.0 41.0 33.5 41.0 20-21 38.248947602410844 40.0 38.0 41.0 34.0 41.0 22-23 38.146951940939886 40.0 38.0 41.0 33.5 41.0 24-25 37.99955851392487 40.0 38.0 41.0 33.0 41.0 26-27 38.06305462977576 40.0 38.0 41.0 33.0 41.0 28-29 38.019517191653435 40.0 38.0 41.0 33.0 41.0 30-31 37.8460461724292 40.0 37.5 41.0 33.0 41.0 32-33 37.605798837076165 40.0 37.0 41.0 33.0 41.0 34-35 37.420005920864654 40.0 36.0 41.0 33.0 41.0 36-37 37.23557511297608 40.0 36.0 41.0 32.0 41.0 38-39 37.06073167033368 39.5 36.0 41.0 31.5 41.0 40-41 36.91583810664365 39.0 36.0 41.0 31.0 41.0 42-43 36.68319041768915 39.0 35.0 41.0 31.0 41.0 44-45 36.38034283250451 39.0 35.0 41.0 30.0 41.0 46-47 35.94250159626098 39.0 35.0 41.0 29.5 41.0 48-49 35.568195673230164 39.0 35.0 40.5 27.5 41.0 50-51 34.88146563061851 38.0 34.0 40.0 26.5 40.5 52-53 35.11913831820625 38.5 35.0 40.0 27.5 40.5 54-55 35.515123992602014 39.0 35.0 40.5 28.0 41.0 56-57 35.412316017383 39.0 35.0 41.0 27.0 41.0 58-59 35.20121862534852 39.0 35.0 41.0 26.5 41.0 60-61 34.922233981430765 38.5 35.0 40.5 26.0 41.0 62-63 34.62670134601677 38.0 34.5 40.0 26.0 41.0 64-65 34.32138948458564 37.5 34.0 40.0 25.5 41.0 66-67 33.94457235162509 37.0 34.0 40.0 24.5 41.0 68-69 33.5616259890887 36.5 34.0 39.0 23.0 41.0 70-71 33.12440572157701 36.0 33.0 39.0 22.0 41.0 72-73 32.739717655213816 35.5 33.0 38.5 21.5 40.0 74-75 32.316098872250635 35.0 33.0 37.5 20.0 39.5 76-77 31.88425586387375 35.0 32.0 37.0 18.5 39.0 78-79 31.43431553669956 35.0 32.0 36.5 16.5 39.0 80-81 30.985046206469008 35.0 31.0 36.0 12.0 38.5 82-83 30.58323920808951 35.0 31.0 36.0 7.5 37.0 84-85 30.216687657885426 34.5 31.0 35.0 4.5 37.0 86-87 29.921850775643584 34.0 31.0 35.0 2.0 36.0 88-89 29.56902913292341 34.0 30.5 35.0 2.0 36.0 90-91 29.213433761100333 34.0 30.0 35.0 2.0 36.0 92-93 28.879913724543634 34.0 29.5 35.0 2.0 35.0 94-95 28.49525041440895 34.0 29.0 35.0 2.0 35.0 96-97 28.079930125545022 34.0 29.0 35.0 2.0 35.0 98-99 27.562286670937116 34.0 29.0 35.0 2.0 35.0 100-101 25.449278613816244 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5192288667150038 1101 2 0.4677749182898765 1101 3 0.4802638123933818 1101 4 0.43106570104545483 1101 5 0.26966566596430397 1101 6 0.28234300736574625 1101 7 0.3251240711093075 1101 8 0.3871199795369549 1101 9 0.42297027939449805 1101 10-11 0.49415581201579784 1101 12-13 0.5384433611539521 1101 14-15 0.7268877236097353 1101 16-17 0.8684713122660241 1101 18-19 0.6809645946899465 1101 20-21 0.6942192902024331 1101 22-23 0.7715681277201085 1101 24-25 0.7611818951497611 1101 26-27 0.7250675349984732 1101 28-29 0.7918026632814374 1101 30-31 0.7945182551565253 1101 32-33 0.8340813942105925 1101 34-35 0.7647909253774401 1101 36-37 0.805337187234727 1101 38-39 0.7546842158666891 1101 40-41 1.087582944583147 1101 42-43 0.9663212561303567 1101 44-45 1.05768576366183 1101 46-47 1.1068754874498907 1101 48-49 1.2957443051948587 1101 50-51 1.1639903210190212 1101 52-53 1.2062575515412775 1101 54-55 1.3572943511534916 1101 56-57 1.3101877802978166 1101 58-59 1.0897364661270146 1101 60-61 1.0652081365824486 1101 62-63 1.190653188059045 1101 64-65 1.1770927312082264 1101 66-67 1.1968050517883526 1101 68-69 1.2085065411205491 1101 70-71 1.1928443072526207 1101 72-73 1.1767145071237906 1101 74-75 1.1655762285495896 1101 76-77 1.027924764357568 1101 78-79 1.1747725249482066 1101 80-81 1.1598770948764106 1101 82-83 1.240901301646879 1101 84-85 1.2402964892673154 1101 86-87 1.2694293658085805 1101 88-89 1.2321672634316592 1101 90-91 1.4223207578237798 1101 92-93 1.286325292918825 1101 94-95 1.10869948221373 1101 96-97 1.1402295312792923 1101 98-99 1.574231922049396 1101 100-101 1.8090856468023624 1102 1 0.44667683514968815 1102 2 0.34085103045517684 1102 3 0.4212655894482893 1102 4 0.41113859041625744 1102 5 0.3200255948821109 1102 6 0.2335854398225976 1102 7 0.3622012675792874 1102 8 0.3945595144907159 1102 9 0.19753128659307606 1102 10-11 0.43081735835476564 1102 12-13 0.5502442105259604 1102 14-15 0.44378179306547594 1102 16-17 0.5211173169044372 1102 18-19 0.28819329262072557 1102 20-21 0.30886643938469405 1102 22-23 0.38862785483676276 1102 24-25 0.44557995544914775 1102 26-27 0.4656898054120191 1102 28-29 0.40261257864543865 1102 30-31 0.4739586334583876 1102 32-33 0.4966969780785462 1102 34-35 0.5134581341964548 1102 36-37 0.557293453612246 1102 38-39 0.6109897488325586 1102 40-41 0.4325394217976921 1102 42-43 0.5927782905570282 1102 44-45 0.47612797925774686 1102 46-47 0.4271158416560823 1102 48-49 0.6635856352752825 1102 50-51 0.6020674873694318 1102 52-53 0.6733368563814963 1102 54-55 0.6824472079951533 1102 56-57 0.8164345800930022 1102 58-59 0.6636830340014725 1102 60-61 0.6913489154746486 1102 62-63 0.5583198246061372 1102 64-65 0.5551024297112761 1102 66-67 0.4121961544659811 1102 68-69 0.30380186355059635 1102 70-71 0.4290902034605679 1102 72-73 0.5710399792481198 1102 74-75 0.6346042698736412 1102 76-77 0.6567220897393291 1102 78-79 0.6453517243699061 1102 80-81 0.5498984797744555 1102 82-83 0.42713665600367534 1102 84-85 0.4254324791471369 1102 86-87 0.1812031075633449 1102 88-89 0.10022244249253376 1102 90-91 0.40201414055321294 1102 92-93 0.5102131276226025 1102 94-95 0.5170456163663175 1102 96-97 0.4855263596880768 1102 98-99 0.8317332021313213 1102 100-101 0.7548824601513537 1103 1 0.10238334062578502 1103 2 0.14323298030256382 1103 3 0.14259541169977297 1103 4 0.07966453451823696 1103 5 0.08165106618500317 1103 6 -0.007500202645530862 1103 7 -0.11122622658552928 1103 8 -0.02703962369827906 1103 9 0.032153346560754414 1103 10-11 0.2041640577383248 1103 12-13 -0.03353997763611005 1103 14-15 0.07838589863732182 1103 16-17 0.005586764507519604 1103 18-19 0.0027842316356370134 1103 20-21 0.2639744649996274 1103 22-23 0.3075188716540467 1103 24-25 0.2542579134358647 1103 26-27 0.2800652916768982 1103 28-29 0.2566583025068283 1103 30-31 0.3073627869086195 1103 32-33 0.3277909992647352 1103 34-35 0.4363140800938794 1103 36-37 0.16775488158519636 1103 38-39 0.13737971831008622 1103 40-41 0.059186737615512186 1103 42-43 0.29457073774118214 1103 44-45 0.4419667487831518 1103 46-47 0.410660637873697 1103 48-49 0.1629093530321768 1103 50-51 0.32508203043006034 1103 52-53 0.20267733157450607 1103 54-55 0.21341255601119258 1103 56-57 0.15655966238398378 1103 58-59 0.2797791498671742 1103 60-61 0.38425393672068253 1103 62-63 0.2752627894753701 1103 64-65 0.13332673996323763 1103 66-67 0.43692534232881286 1103 68-69 0.33229848213937885 1103 70-71 0.19160441735727574 1103 72-73 0.020797594904351513 1103 74-75 0.0069006371008910605 1103 76-77 0.3612944758785339 1103 78-79 0.19707235397072154 1103 80-81 -0.015135334337699646 1103 82-83 0.04241584685890132 1103 84-85 -0.11899268094622428 1103 86-87 -0.09500731795583661 1103 88-89 0.055144939350512345 1103 90-91 0.15421654644225313 1103 92-93 0.024459940717338924 1103 94-95 -0.10764766270484216 1103 96-97 -0.06726255646489676 1103 98-99 0.20683344750806754 1103 100-101 0.3156670672493256 1104 1 0.46557955569885223 1104 2 0.338437991332583 1104 3 0.3823816853774815 1104 4 0.3644353942283374 1104 5 0.3976479237697603 1104 6 0.39939171720885014 1104 7 0.3938538944816301 1104 8 0.38604418690106 1104 9 0.3850157150861051 1104 10-11 0.508866106971837 1104 12-13 0.5063443211598866 1104 14-15 0.5597400832051207 1104 16-17 0.6607970012057436 1104 18-19 0.5965762079552874 1104 20-21 0.6911070812356783 1104 22-23 0.5693923613833292 1104 24-25 0.7774568991652302 1104 26-27 0.623158466393086 1104 28-29 0.5455851466591639 1104 30-31 0.7573857588057464 1104 32-33 0.732630353807032 1104 34-35 0.8924272350998166 1104 36-37 0.7757096462372317 1104 38-39 0.8762620215321135 1104 40-41 0.768374795034731 1104 42-43 0.672088440130608 1104 44-45 0.8833927950566149 1104 46-47 0.9329968622758074 1104 48-49 0.9342872460006717 1104 50-51 0.7114465435836621 1104 52-53 0.6877824236199146 1104 54-55 0.9460547450400938 1104 56-57 0.8593971300742709 1104 58-59 0.888918457764241 1104 60-61 0.8730520681059062 1104 62-63 0.905044363558865 1104 64-65 1.0101789587679306 1104 66-67 0.9956781612085521 1104 68-69 0.9588683745747844 1104 70-71 1.1497543554559044 1104 72-73 1.0668737844453062 1104 74-75 1.0060001738050701 1104 76-77 1.0008733010501238 1104 78-79 0.9756758730549286 1104 80-81 1.0169016942561733 1104 82-83 0.9773413723191275 1104 84-85 0.8714169903978224 1104 86-87 0.9746299715707352 1104 88-89 1.0430355931201838 1104 90-91 1.0668944032870016 1104 92-93 0.984310851351399 1104 94-95 1.0467077037758266 1104 96-97 1.1895536087363645 1104 98-99 1.6073432131947136 1104 100-101 1.6258245036557497 1105 1 0.15189898310762118 1105 2 0.16497949986805338 1105 3 0.2619091677365333 1105 4 0.17883567417465684 1105 5 0.27820355949322106 1105 6 0.20932945079808007 1105 7 0.2251880407175051 1105 8 0.2005977879631189 1105 9 0.15714626531049447 1105 10-11 0.18275389756044547 1105 12-13 0.09770657025438823 1105 14-15 0.21713503118417066 1105 16-17 0.10011791492744493 1105 18-19 0.2984405927227485 1105 20-21 0.29270486763952164 1105 22-23 0.17235019627272408 1105 24-25 0.22235599583329702 1105 26-27 0.11354703621371698 1105 28-29 0.26288626211538 1105 30-31 -0.1399783806479249 1105 32-33 -0.1548139094579497 1105 34-35 -0.20934281208700867 1105 36-37 -0.06690713033962936 1105 38-39 -0.12277656488327438 1105 40-41 -0.13644165253305118 1105 42-43 -0.019722588888569703 1105 44-45 -0.15412758763288537 1105 46-47 -0.4274141826166087 1105 48-49 -0.2484201162989308 1105 50-51 -0.5058717077619619 1105 52-53 -0.48495455671169196 1105 54-55 -0.46148901461011604 1105 56-57 -0.214954592172802 1105 58-59 -0.136056650101672 1105 60-61 -0.4137206486721112 1105 62-63 -0.4015374413733852 1105 64-65 -0.18316708518653257 1105 66-67 -0.009171295493565879 1105 68-69 -0.15303890402952192 1105 70-71 -0.2055005905029148 1105 72-73 -0.0418236715064495 1105 74-75 -0.26283126676406 1105 76-77 -0.19141392531678036 1105 78-79 -0.19925991245424157 1105 80-81 -0.038901780541440445 1105 82-83 -0.07831238891840542 1105 84-85 -0.15164521107694284 1105 86-87 -0.04895352169756606 1105 88-89 -0.17669430918720863 1105 90-91 -0.041232156980822054 1105 92-93 -0.1572830028167722 1105 94-95 0.1241862748951803 1105 96-97 0.08475772423586747 1105 98-99 0.08456149917286737 1105 100-101 0.15986539890676355 1106 1 0.1956742196309449 1106 2 0.26051724044396707 1106 3 0.21099033723755412 1106 4 0.229378719946709 1106 5 0.15808139261532972 1106 6 0.24590951504989533 1106 7 0.15806625699266874 1106 8 0.23105845582836793 1106 9 0.22751011334609927 1106 10-11 0.3639005567605409 1106 12-13 0.3463489558242543 1106 14-15 0.483571928823352 1106 16-17 0.5228609960674717 1106 18-19 0.5119415800786911 1106 20-21 0.4085239309776654 1106 22-23 0.08259659316033918 1106 24-25 0.4603327266535331 1106 26-27 0.3592973530518222 1106 28-29 0.32551704993699815 1106 30-31 0.3411089635119353 1106 32-33 0.3779088350968607 1106 34-35 0.48389343124465256 1106 36-37 0.4699787195233114 1106 38-39 0.5522433296022697 1106 40-41 0.614663596663803 1106 42-43 0.4631699956261528 1106 44-45 0.579621565009397 1106 46-47 0.539885177812522 1106 48-49 0.48250144048139987 1106 50-51 0.6371714853766548 1106 52-53 0.5130117847660998 1106 54-55 0.6618531115946098 1106 56-57 0.584372679482712 1106 58-59 0.6883560941584008 1106 60-61 0.6002883848979934 1106 62-63 0.6548947405358874 1106 64-65 0.5481339404847247 1106 66-67 0.578804698493876 1106 68-69 0.5963071568623377 1106 70-71 0.5443723058394454 1106 72-73 0.5747764345974815 1106 74-75 0.5065621724767126 1106 76-77 0.6892541609810756 1106 78-79 0.6601044955742843 1106 80-81 0.9082525711930636 1106 82-83 0.7296431948005342 1106 84-85 0.8286831953010783 1106 86-87 0.5795777494863756 1106 88-89 0.7916753008808755 1106 90-91 0.8176698884670266 1106 92-93 0.773732868561698 1106 94-95 0.7516638751781244 1106 96-97 0.554691261917494 1106 98-99 0.722183445000919 1106 100-101 0.7130684110792416 1107 1 0.21991192827930206 1107 2 0.31422629985644335 1107 3 0.2672171235023626 1107 4 0.3133849151696353 1107 5 0.303059885502563 1107 6 0.14092103933899125 1107 7 0.17022548303022234 1107 8 0.15637812639518955 1107 9 0.40432720746356665 1107 10-11 0.44236481678373707 1107 12-13 0.36827263126504306 1107 14-15 0.43623242473480417 1107 16-17 0.5156876766317922 1107 18-19 0.48786168812807773 1107 20-21 0.660579218204056 1107 22-23 0.46211088474687756 1107 24-25 0.5320695911660636 1107 26-27 0.4619002275178161 1107 28-29 0.5618769160497195 1107 30-31 0.608466965185535 1107 32-33 0.7200591059543484 1107 34-35 0.8194300814200801 1107 36-37 0.7601797706559239 1107 38-39 0.48548686645797545 1107 40-41 0.8688989564124654 1107 42-43 1.0616219239259834 1107 44-45 0.8076391670764309 1107 46-47 0.7162416673819365 1107 48-49 1.0252697655691208 1107 50-51 1.0433384481025527 1107 52-53 1.1136217646536508 1107 54-55 1.2690932278651417 1107 56-57 1.2260773934307707 1107 58-59 1.2821652754200557 1107 60-61 1.2622890602066548 1107 62-63 1.1201787371887164 1107 64-65 1.1871979773624517 1107 66-67 1.1447757483052357 1107 68-69 0.9739184726865844 1107 70-71 0.8325639466926873 1107 72-73 0.9038994875798494 1107 74-75 0.9947442709249614 1107 76-77 1.0061867916019231 1107 78-79 0.9810944833893736 1107 80-81 0.8379195412592715 1107 82-83 1.067745283853192 1107 84-85 0.9585828532425644 1107 86-87 0.7792599139974392 1107 88-89 0.9113187085470962 1107 90-91 0.9065427102295835 1107 92-93 1.1006281369968853 1107 94-95 1.1059458459049623 1107 96-97 1.1047668671261803 1107 98-99 0.8494295803269019 1107 100-101 0.6553919132886747 1108 1 -0.10955139857177087 1108 2 -0.040173171887666115 1108 3 -0.13312900020239482 1108 4 -0.18948493651236475 1108 5 -0.13305598249151274 1108 6 -0.2782659690492508 1108 7 -0.1354989821832575 1108 8 -0.2268831280674135 1108 9 -0.19495297133072853 1108 10-11 -0.22373465205787113 1108 12-13 -0.08979342974561177 1108 14-15 0.024386939581113154 1108 16-17 0.09534692256102772 1108 18-19 0.009795554554806074 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-3.047799672797776 2316 98-99 -2.869676883551424 2316 100-101 -2.6806347026809085 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1705.0 3 520.0 4 526.0 5 473.0 6 606.0 7 688.0 8 835.0 9 1072.0 10 1446.0 11 1944.0 12 2952.0 13 3883.0 14 4521.0 15 5923.0 16 7943.0 17 11535.0 18 9454.0 19 5259.0 20 4684.0 21 4816.0 22 5259.0 23 5815.0 24 6564.0 25 7736.0 26 8875.0 27 10420.0 28 12706.0 29 15126.0 30 18991.0 31 23511.0 32 30544.0 33 41168.0 34 57490.0 35 86687.0 36 130266.0 37 199827.0 38 200997.0 39 36681.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.65874039570817 20.612776004078018 15.423311161513077 21.30517243870073 2 14.47155394354643 21.971695825572816 40.656887985430004 22.89986224545075 3 17.51519972432606 27.999991746289183 31.895433737661993 22.589374791722765 4 11.303542921535222 18.19982065075501 40.5671894074561 29.92944702025367 5 11.54200654170206 38.870075273955706 35.836926039958264 13.750992144383972 6 27.238254479398893 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19.929849175727814 28-29 19.343342432514408 27.512127083165687 33.063143364716225 20.08138711960368 30-31 19.795995213237873 27.37754554645429 32.52047785090886 20.305981389398973 32-33 19.665282972933383 27.72296071946572 32.35780174254631 20.25395456505458 34-35 20.355366290957182 27.41183412663535 32.03377258950695 20.199026992900514 36-37 20.50844301768962 26.828985244125164 32.19183204638283 20.47073969180239 38-39 19.829417708434878 27.74984771280363 32.255124509289644 20.165610069471846 40-41 19.588896534009496 27.747730276018657 32.591131958105656 20.07224123186619 42-43 19.53740380846279 27.815755812754016 31.574085535681128 21.07275484310206 44-45 19.84802589944829 27.62368848455528 31.434697194209242 21.093588421787185 46-47 20.53843035436324 28.007982018280863 30.99221294184515 20.46137468551075 48-49 20.307370633922275 28.13006570230394 30.691625186897497 20.870938476876287 50-51 19.935950369530985 28.173842807620552 30.921870075907425 20.968336746941034 52-53 20.262696002050735 28.70525820099762 30.19755841055818 20.83448738639347 54-55 20.077976630736966 28.22596201953181 30.392523981501828 21.303537368229396 56-57 20.45430394021024 28.636861202790158 29.703444782272715 21.20539007472688 58-59 20.704079622108587 27.947067142985354 30.134189001412242 21.214664233493814 60-61 20.55706441137858 28.478273765768837 30.52505603400471 20.439605788847878 62-63 20.310934816136836 28.50381962892086 30.73916654201076 20.446079012931545 64-65 20.246500304321362 28.565253721489213 30.392368239165645 20.79587773502378 66-67 20.247321917232323 28.622201405442155 29.59898525074964 21.531491426575887 68-69 19.993355546854485 28.566247725641862 30.379646925728114 21.060749801775533 70-71 20.625996544881207 28.420595974461627 30.06298963206441 20.890417848592755 72-73 20.47789175476298 28.058421601590428 30.272650647529815 21.19103599611678 74-75 19.92483200427527 29.395112223259055 29.614240445767052 21.065815326698615 76-77 20.157165430208508 28.476007071567377 30.036982832310272 21.32984466591384 78-79 20.191372550638246 28.466486328357774 30.00264677338718 21.339494347616792 80-81 20.402717941125033 29.059900050506123 29.50099026041863 21.03639174795022 82-83 20.268742093848214 28.59555790346911 30.023205135877472 21.112494866805203 84-85 20.007655154875028 28.534383458262642 29.568087289399415 21.889874097462915 86-87 19.994294522328744 28.720115884132376 29.9133654683444 21.37222412519448 88-89 20.457809324350816 28.15323587368074 29.62926471953698 21.759690082431472 90-91 20.19049928040483 27.79876095512718 30.28345343780789 21.727286326660096 92-93 20.56138265551702 28.341425237972157 30.024139000194495 21.073053106316326 94-95 19.971060471234573 27.774416115345428 30.25826337034601 21.99626004307399 96-97 19.864001382687004 27.92981315667959 30.2635102192436 21.942675241389807 98-99 19.83195454113878 28.399403899422477 29.62153392695704 22.147107632481703 100-101 19.6970119199119 28.06716600456402 30.163858794095884 22.0719632814282 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1426.0 1 1009.0 2 703.0 3 786.5 4 644.5 5 682.5 6 1211.0 7 2638.5 8 4465.0 9 4855.0 10 3575.5 11 2469.0 12 2335.0 13 2369.0 14 2307.0 15 2187.0 16 2111.0 17 2158.0 18 2153.0 19 2173.0 20 2365.5 21 2707.0 22 3136.0 23 3616.0 24 3937.0 25 4796.5 26 6639.0 27 8329.0 28 8750.0 29 9830.5 30 12958.0 31 16067.0 32 19997.0 33 24846.5 34 29479.5 35 32934.5 36 37797.5 37 42335.5 38 41576.0 39 38822.5 40 35734.0 41 33575.5 42 31577.5 43 31136.5 44 31993.0 45 32220.0 46 30978.0 47 32082.5 48 42264.5 49 40823.0 50 28445.5 51 23140.5 52 20989.0 53 20318.5 54 19558.0 55 18000.5 56 16227.0 57 14377.0 58 13274.5 59 12401.0 60 10848.0 61 9070.0 62 7354.5 63 5701.0 64 4118.5 65 3115.5 66 2308.0 67 1730.5 68 1191.5 69 707.5 70 535.5 71 360.0 72 224.0 73 177.5 74 126.0 75 103.0 76 80.5 77 37.0 78 16.5 79 13.0 80 10.5 81 10.5 82 6.0 83 4.5 84 4.0 85 3.5 86 4.0 87 2.5 88 2.5 89 1.5 90 1.0 91 0.5 92 0.0 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03661858800787661 2 0.03486502182158392 3 0.019804982809893828 4 0.040641475141136287 5 0.0610653636638393 6 0.05910549557327689 7 0.059208646525411746 8 0.060549608903164985 9 0.059827552238220935 10-11 0.0656040055577733 12-13 0.0664292131748522 14-15 0.060652759855299845 16-17 0.050595542022150636 18-19 0.04409703203765422 20-21 0.05193650439990386 22-23 0.027902332552480626 24-25 0.015472642820229553 26-27 0.011656057591239596 28-29 0.013925378538206596 30-31 0.011656057591239596 32-33 0.006911113793035867 34-35 0.00897413283573314 36-37 0.004383915465731706 38-39 0.006962689269103298 40-41 0.006395359032361549 42-43 0.0034039814204505016 44-45 0.0011862359495509324 46-47 0.0024756228512367285 48-49 4.641792846068865E-4 50-51 0.005002821178540889 52-53 0.00448706641786657 54-55 0.0061890571280918205 56-57 0.0195986809056241 58-59 0.0076331704579799126 60-61 0.018102992099668577 62-63 0.012171812351913915 64-65 0.008561529027193687 66-67 0.01542106734416212 68-69 0.02305423780214203 70-71 0.017174633530454803 72-73 0.015885246628769008 74-75 0.01624627496124103 76-77 0.02264163399360258 78-79 0.03594810681899999 80-81 0.02718027588753658 82-83 0.027953908028548056 84-85 0.017690388291129122 86-87 0.02155854899618651 88-89 0.01681360519798278 90-91 0.016297850437308463 92-93 0.02831493636102008 94-95 0.01965025638169153 96-97 0.03357563491989813 98-99 0.050440815593948335 100-101 0.058796042716872304 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 969453.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.644334483560634 #Duplication Level Percentage of deduplicated Percentage of total 1 71.66492351716822 26.261134281019032 2 13.8496028512618 10.15018958692225 3 5.220217610287977 5.738742005650987 4 2.5430703033248516 3.727564752409834 5 1.4685593396760088 2.6907189826022306 6 0.9403955602300124 2.0676101673554377 7 0.6589368573804277 1.6902411823776282 8 0.43764638650987425 1.2829808458231593 9 0.33607812597715697 1.108382333482365 >10 2.3670106548763807 17.39947224149645 >50 0.30734471092761917 7.845507138318221 >100 0.19124237502917246 12.67476182673341 >500 0.009604491507850584 2.2376786645664968 >1k 0.004802245753925292 3.4506646751780625 >5k 5.649700886970931E-4 1.6743513160643513 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9481 0.9779741771906426 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6694 0.6904924735907775 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4930 0.5085341940248779 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4196 0.432821395157888 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3251 0.3353437453904418 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2538 0.261797116518284 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2002 0.2065082061739971 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1983 0.2045483380834347 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1832 0.18897254431107027 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1480 0.15266340915959825 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1406 0.14503023870161832 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1354 0.13966638919060542 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1316 0.1357466530094806 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1287 0.13275527539756957 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1220 0.12584416160453368 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1182 0.12192442542340887 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1144 0.11800468924228404 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1132 0.1167668778166657 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1082 0.1116093302099225 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0315095213486368E-4 0.0 12-13 0.0 0.0 0.0 2.0630190426972736E-4 0.0 14-15 0.0 0.0 0.0 2.0630190426972736E-4 0.0 16-17 0.0 0.0 0.0 3.0945285640459106E-4 0.0 18-19 0.0 0.0 0.0 3.0945285640459106E-4 0.0 20-21 0.0 0.0 0.0 3.610283324720229E-4 0.0 22-23 0.0 0.0 0.0 0.001031509521348637 0.0 24-25 0.0 0.0 0.0 0.002011443566629842 0.0 26-27 0.0 0.0 0.0 0.002939802135843615 0.0 28-29 0.0 0.0 0.0 0.004229189037529411 0.0 30-31 0.0 0.0 0.0 0.0076847459340473444 0.0 32-33 0.0 0.0 0.0 0.01232653878011621 0.0 34-35 0.0 0.0 0.0 0.015936822104836438 0.0 36-37 0.0 0.0 0.0 0.020423888522703007 0.0 38-39 0.0 0.0 0.0 0.02465307756023242 0.0 40-41 0.0 0.0 0.0 0.03063583278405451 0.0 42-43 0.0 0.0 0.0 0.03914578633518077 0.0 44-45 0.0 0.0 0.0 0.05147232511529698 0.0 46-47 0.0 0.0 0.0 0.08819406407530844 0.0 48-49 0.0 0.0 0.0 0.16401001389443326 0.0 50-51 0.0 0.0 0.0 0.209241706405571 0.0 52-53 0.0 0.0 0.0 0.2529261346346857 0.0 54-55 0.0 0.0 0.0 0.3112064225908837 0.0 56-57 0.0 0.0 0.0 0.35246680344482917 0.0 58-59 0.0 0.0 0.0 0.4090966761668694 0.0 60-61 0.0 0.0 0.0 0.5052849390326297 0.0 62-63 0.0 0.0 0.0 0.5672786612656828 0.0 64-65 0.0 0.0 0.0 0.6445902998907631 0.0 66-67 0.0 0.0 0.0 0.7745089241046239 0.0 68-69 0.0 0.0 0.0 0.9251608896975924 0.0 70-71 0.0 0.0 0.0 1.04992196630471 0.0 72-73 0.0 0.0 0.0 1.2032042811771175 0.0 74-75 0.0 0.0 0.0 1.3353406508618777 0.0 76-77 0.0 0.0 0.0 1.4292595927806713 0.0 78-79 0.0 0.0 0.0 1.5570120469996998 0.0 80-81 0.0 0.0 0.0 1.7299961937298662 0.0 82-83 0.0 0.0 0.0 1.9211349080357687 0.0 84-85 0.0 0.0 0.0 2.1743189200507915 0.0 86-87 0.0 0.0 0.0 2.597959880468677 0.0 88-89 0.0 0.0 0.0 3.125164396829965 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGGGA 115 3.859732E-6 28.916906 2 GTACATG 5210 0.0 25.348875 1 ACACCGA 95 0.0012247243 25.00592 6 CCCTTAC 95 0.0012254614 25.00334 1 GAGTACT 5440 0.0 24.325825 12-13 CAACGCT 100 0.0016516779 23.754398 4 ACATGGG 5270 0.0 23.527811 3 TACATGG 5430 0.0 23.271986 2 CATGGGG 1575 0.0 22.92488 4 GTATAGG 415 0.0 22.894625 1 CTCCCGC 215 4.40632E-8 22.099396 7 GTACTTT 6010 0.0 21.937958 14-15 GTATCAA 12555 0.0 21.908543 1 AGTACTT 5990 0.0 21.89392 12-13 CAACGCG 110 0.0028739548 21.594908 4 AAACGGT 55 0.0028792873 21.589336 66-67 CATGGGA 3270 0.0 21.357164 4 TACACTG 290 1.05501385E-10 21.299244 5 GACAGTA 180 5.154481E-6 21.117199 7 ATGGGAT 1425 0.0 20.672628 5 >>END_MODULE