FastQCFastQC Report
Thu 26 May 2016
SRR937066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937066_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969453
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86080.8879233959769065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65750.6782175102867287No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT57740.5955935976267029No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48750.5028608916574605No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28870.29779679881335147No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23420.24157952989985074No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22700.23415266134614054No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21690.2237344151805193No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17400.17948265671466282No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16620.17143688244814345No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15640.1613280891389268No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15240.15720205105353224No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14340.14791846536139452No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14100.1454428425101578No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12940.1334773320625136No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG12000.12378114256183642No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA11810.121821274471274No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT10940.11284714163554087No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC10020.1033572540391334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACG300.003950245731.6689136-37
TAGTGCG650.005840866429.2335975
ACCGTCC700.00839542327.1398818
ATGGGGA11000.026.3434565
CATGGGG18100.025.9831754
GTACATG56100.025.2432821
GTATGGT3250.024.8575331
GAGTACT58950.023.68758212-13
ACATGGG57000.023.6689553
TACATGG59550.023.1364442
GTATAGG7200.022.4408281
ATGGGAT13250.022.2285655
GTACTTT62600.022.15583414-15
ATGGGTA4150.021.749095
AGTACTT63050.021.65758112-13
TATAGGG3750.021.5376382
TCGAAAT1005.389029E-721.37541220-21
GTAAGGT2901.05501385E-1021.2951624
TAGGACA5150.021.2156434
AGGGGTC4850.020.5689965
Produced by FastQC (version 0.11.5)