##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937066_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 969453 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.228911561468166 33.0 31.0 34.0 31.0 34.0 2 32.29995677975106 33.0 31.0 34.0 31.0 34.0 3 32.424995332419414 34.0 31.0 34.0 31.0 34.0 4 36.099719119957335 37.0 35.0 37.0 35.0 37.0 5 36.02289951137394 37.0 35.0 37.0 35.0 37.0 6 36.07298445618302 37.0 35.0 37.0 35.0 37.0 7 36.02686566548353 37.0 35.0 37.0 35.0 37.0 8 36.01635148893242 37.0 35.0 37.0 35.0 37.0 9 37.6948083094281 39.0 37.0 39.0 35.0 39.0 10-11 37.60154798633869 39.0 37.0 39.0 35.0 39.0 12-13 37.54443639867018 39.0 37.0 39.0 35.0 39.0 14-15 38.717257566895974 40.0 38.0 41.0 34.5 41.0 16-17 38.66500542058253 40.0 38.0 41.0 34.5 41.0 18-19 38.752607913947344 40.0 38.0 41.0 34.5 41.0 20-21 38.852400271080704 40.0 38.0 41.0 35.0 41.0 22-23 38.77468324921374 40.0 38.0 41.0 35.0 41.0 24-25 38.676082285577536 40.0 38.0 41.0 34.0 41.0 26-27 38.48773896207449 40.0 38.0 41.0 34.0 41.0 28-29 38.34254110307565 40.0 38.0 41.0 34.0 41.0 30-31 38.25093635276801 40.0 37.5 41.0 34.0 41.0 32-33 38.117738044031015 40.0 37.0 41.0 34.0 41.0 34-35 37.972559267958324 40.0 37.0 41.0 33.0 41.0 36-37 37.77998056636062 40.0 37.0 41.0 33.0 41.0 38-39 37.61976702325951 40.0 37.0 41.0 33.0 41.0 40-41 37.41326088010455 40.0 36.0 41.0 33.0 41.0 42-43 37.15918719112737 40.0 36.0 41.0 32.5 41.0 44-45 36.85902514098156 40.0 36.0 41.0 31.5 41.0 46-47 36.428425617332664 39.5 35.5 41.0 30.5 41.0 48-49 35.99777554971721 39.0 35.0 41.0 29.5 41.0 50-51 35.74648487342863 39.0 35.0 41.0 28.5 41.0 52-53 35.55265907681961 39.0 35.0 40.0 28.0 41.0 54-55 35.303683623651686 39.0 35.0 40.0 27.0 41.0 56-57 35.06515529891598 38.0 35.0 40.0 27.0 41.0 58-59 34.751747635006545 38.0 34.0 40.0 26.0 41.0 60-61 34.41283899271032 38.0 34.0 40.0 26.0 41.0 62-63 34.10426704543696 37.0 34.0 40.0 25.0 41.0 64-65 33.703225427122305 37.0 33.0 39.5 24.0 41.0 66-67 33.268139352810294 36.0 33.0 39.0 22.5 40.0 68-69 32.82724691140262 36.0 32.5 39.0 22.0 40.0 70-71 32.42757410622279 35.0 32.0 38.0 21.0 40.0 72-73 32.04166163805775 35.0 32.0 37.5 20.0 39.5 74-75 31.572769386447824 35.0 31.0 37.0 18.5 39.0 76-77 30.089794966852445 33.5 29.5 35.5 16.0 38.0 78-79 30.78429743370746 34.0 31.0 36.0 15.5 38.0 80-81 30.466632730003415 34.0 31.0 36.0 9.5 37.0 82-83 30.270720189632712 34.0 31.0 35.0 7.5 37.0 84-85 29.931533555520485 34.0 30.0 35.0 6.0 36.5 86-87 29.599276602372676 34.0 30.0 35.0 2.0 36.0 88-89 29.219108610732032 34.0 29.5 35.0 2.0 36.0 90-91 28.783830675649053 34.0 29.0 35.0 2.0 35.0 92-93 28.387761448982054 34.0 29.0 35.0 2.0 35.0 94-95 27.927579779525153 33.5 29.0 35.0 2.0 35.0 96-97 27.329840642094048 33.0 27.0 35.0 2.0 35.0 98-99 26.64532937646281 33.0 25.5 35.0 2.0 35.0 100-101 23.523385868113255 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3749170177499508 1101 2 0.3599020649635065 1101 3 0.27070343193933155 1101 4 0.20369670475771073 1101 5 0.2511205136387389 1101 6 0.29761597243803806 1101 7 0.2955629656421266 1101 8 0.33738667219429885 1101 9 0.43433377384572225 1101 10-11 0.42449610772747803 1101 12-13 0.31176706089529915 1101 14-15 0.4916852920867498 1101 16-17 0.49259240937150395 1101 18-19 0.5480147536622084 1101 20-21 0.4190115165790118 1101 22-23 0.5016924832936311 1101 24-25 0.5612064762102733 1101 26-27 0.5109207112508685 1101 28-29 0.4777131927931393 1101 30-31 0.3728904602389349 1101 32-33 0.5780543202435169 1101 34-35 0.6522963737043526 1101 36-37 0.6732486411815302 1101 38-39 0.7223131293733474 1101 40-41 0.7924346104904529 1101 42-43 0.7008329817823977 1101 44-45 0.7895263546470304 1101 46-47 0.7402816008200688 1101 48-49 0.6655358603532378 1101 50-51 0.7528065435531417 1101 52-53 0.7158949044188958 1101 54-55 0.7251104989454547 1101 56-57 0.7404759135532188 1101 58-59 0.7937465596119324 1101 60-61 0.5855005946289467 1101 62-63 0.5680258105397229 1101 64-65 0.8871195575671464 1101 66-67 0.686672838479403 1101 68-69 0.7293402554669228 1101 70-71 0.7005272258129267 1101 72-73 0.7528007827473076 1101 74-75 0.817327246916161 1101 76-77 0.7696638416916812 1101 78-79 0.9027997941635562 1101 80-81 1.1058068476375027 1101 82-83 1.022242989848305 1101 84-85 0.9500817318353434 1101 86-87 0.8001030526354462 1101 88-89 0.6879776775267175 1101 90-91 0.6753553366369367 1101 92-93 0.8334460999596764 1101 94-95 1.0931161672488017 1101 96-97 1.1022742546795392 1101 98-99 1.327925434065719 1101 100-101 1.5124176200913517 1102 1 0.28887744062878085 1102 2 0.21233422062146445 1102 3 0.17157388764849912 1102 4 0.19072119197195292 1102 5 0.2552529192891697 1102 6 0.33274142046671074 1102 7 0.3470312949093213 1102 8 0.2778222012446321 1102 9 0.2920549268230417 1102 10-11 0.30465031994999947 1102 12-13 0.30333659193352247 1102 14-15 0.33731042385988275 1102 16-17 0.4333863613609026 1102 18-19 0.481284835888637 1102 20-21 0.3070630812909272 1102 22-23 0.4800244612461029 1102 24-25 0.355071118841515 1102 26-27 0.39220741948255267 1102 28-29 0.26704182747281635 1102 30-31 0.4577885054785398 1102 32-33 0.5038999273035429 1102 34-35 0.5191558056491701 1102 36-37 0.5500324729938271 1102 38-39 0.36920629524184534 1102 40-41 0.46792330059014375 1102 42-43 0.2772132112935637 1102 44-45 0.3688763742247545 1102 46-47 0.5089120637579398 1102 48-49 0.6006402246796156 1102 50-51 0.6771461718775882 1102 52-53 0.6470234848833414 1102 54-55 0.5395859665518543 1102 56-57 0.42675042335714153 1102 58-59 0.42608293502232186 1102 60-61 0.39173534659424547 1102 62-63 0.2839590655621578 1102 64-65 0.37750538931921085 1102 66-67 0.318253212564521 1102 68-69 0.33156609203810916 1102 70-71 0.44581200639182583 1102 72-73 0.4692912499019144 1102 74-75 0.6944484179652228 1102 76-77 0.6293548148551409 1102 78-79 0.5729000923473428 1102 80-81 0.6527301330807127 1102 82-83 0.6728107438316222 1102 84-85 0.5018934242542485 1102 86-87 0.5099069742040747 1102 88-89 0.5386689217663481 1102 90-91 0.5075079826415738 1102 92-93 0.6461234289557893 1102 94-95 0.6603882373671723 1102 96-97 0.8487156269273655 1102 98-99 0.8328650443184209 1102 100-101 0.7977554111217451 1103 1 0.1864416275938865 1103 2 0.1506668557282964 1103 3 0.0686832299191309 1103 4 0.11831709836970106 1103 5 0.07880310182552819 1103 6 0.09404969744318947 1103 7 0.10532323338559024 1103 8 0.176613271925099 1103 9 0.1461275822781829 1103 10-11 0.14340488335894008 1103 12-13 0.21267287776750265 1103 14-15 0.26696510098000203 1103 16-17 0.32805689819733175 1103 18-19 0.34165948428172754 1103 20-21 0.2750114923268896 1103 22-23 0.09282893787546698 1103 24-25 0.13812815397228206 1103 26-27 0.2647222318591176 1103 28-29 0.16552468102479878 1103 30-31 0.17187672141006516 1103 32-33 0.22348218836045675 1103 34-35 0.3770377854205478 1103 36-37 0.4788193543152701 1103 38-39 0.4437319999518863 1103 40-41 0.5338099485044481 1103 42-43 0.31232303234808256 1103 44-45 0.5513977698797916 1103 46-47 0.4394764302670069 1103 48-49 0.3898346707865059 1103 50-51 0.6020219873063937 1103 52-53 0.5156511705617177 1103 54-55 0.3395380881629677 1103 56-57 0.3966020003515709 1103 58-59 0.46029802868441294 1103 60-61 0.400790346893487 1103 62-63 0.2768790488047159 1103 64-65 0.31409944042938776 1103 66-67 0.2412946872189039 1103 68-69 0.46677765466903054 1103 70-71 0.3868138616793928 1103 72-73 0.5280290558747396 1103 74-75 0.3011753316045791 1103 76-77 0.41797771269462913 1103 78-79 0.5011573189916696 1103 80-81 0.6426397626216804 1103 82-83 0.6054368734623559 1103 84-85 0.5610352041333542 1103 86-87 0.3748420391391427 1103 88-89 0.4560667313300577 1103 90-91 0.5929738992134794 1103 92-93 0.7018389116297961 1103 94-95 0.6207090225727505 1103 96-97 0.331814555648414 1103 98-99 0.3596320561084738 1103 100-101 0.7930723062082272 1104 1 0.2659910276941915 1104 2 0.33765283761030673 1104 3 0.28570628248093044 1104 4 0.19876959213572576 1104 5 0.20411217028879491 1104 6 0.21022436803320232 1104 7 0.21509032289883123 1104 8 0.2913535141295611 1104 9 0.3561433294259828 1104 10-11 0.37395768189715284 1104 12-13 0.3125375013966405 1104 14-15 0.45559681762954085 1104 16-17 0.41566838204160206 1104 18-19 0.5027626263462466 1104 20-21 0.4017304390566423 1104 22-23 0.3445649820095653 1104 24-25 0.38533820829819376 1104 26-27 0.41292253277342894 1104 28-29 0.42410933158892306 1104 30-31 0.5272256662810193 1104 32-33 0.4545023159509398 1104 34-35 0.4598137725983733 1104 36-37 0.5104390308849887 1104 38-39 0.616727921124955 1104 40-41 0.5806287791471973 1104 42-43 0.646765797252634 1104 44-45 0.5114591101058608 1104 46-47 0.3455994053205771 1104 48-49 0.5864346546535657 1104 50-51 0.5549392428836555 1104 52-53 0.6114816835539898 1104 54-55 0.6469862435722362 1104 56-57 0.6059294673755034 1104 58-59 0.6457513817046063 1104 60-61 0.5385156380754381 1104 62-63 0.46603157869449774 1104 64-65 0.6162316182704473 1104 66-67 0.4010975497627882 1104 68-69 0.4765792897069616 1104 70-71 0.36240665937589256 1104 72-73 0.42988031021046424 1104 74-75 0.40379132214927793 1104 76-77 0.4397326253488103 1104 78-79 0.5562048234720649 1104 80-81 0.6762664114252104 1104 82-83 0.583088374000706 1104 84-85 0.4112750938682481 1104 86-87 0.6247868013712363 1104 88-89 0.830362230591799 1104 90-91 0.7625135143377584 1104 92-93 0.9833709092028435 1104 94-95 0.9509090008283607 1104 96-97 1.089746874477303 1104 98-99 1.1703050450153256 1104 100-101 1.3432176779847076 1105 1 0.15995775160074288 1105 2 0.16426108592558109 1105 3 0.18740039062102198 1105 4 0.1480672010478301 1105 5 0.11922818927896373 1105 6 0.0753180201782584 1105 7 0.011048778287793937 1105 8 0.09368652037785097 1105 9 -0.016758876955059065 1105 10-11 -0.016769457022604684 1105 12-13 0.01657092900742896 1105 14-15 0.24798469479949148 1105 16-17 0.14560405932409992 1105 18-19 0.04363533013707155 1105 20-21 0.04848760726974177 1105 22-23 -0.06055359834513041 1105 24-25 0.18495292135321506 1105 26-27 0.08838051196504892 1105 28-29 0.08620582648784136 1105 30-31 0.1431923468969032 1105 32-33 0.01702125561818235 1105 34-35 0.10543997211063783 1105 36-37 0.056997242403191706 1105 38-39 0.13365998363668297 1105 40-41 0.14812434185373036 1105 42-43 0.20093053988960463 1105 44-45 0.22541241390533884 1105 46-47 0.21597181329183002 1105 48-49 0.2531497226920649 1105 50-51 0.25376786955936126 1105 52-53 0.3074765752551727 1105 54-55 0.17533982731803377 1105 56-57 0.11649960547273253 1105 58-59 0.41861336383999515 1105 60-61 0.4736148097806563 1105 62-63 0.458591897025201 1105 64-65 0.4698568509577896 1105 66-67 0.2492690601741927 1105 68-69 0.236629939788493 1105 70-71 0.5332983749850655 1105 72-73 0.41255953357588027 1105 74-75 0.22183523331682764 1105 76-77 0.27821153557840006 1105 78-79 0.2994496754901341 1105 80-81 0.5957556897697778 1105 82-83 0.45971447971651713 1105 84-85 0.14937557150861736 1105 86-87 0.11974884988564582 1105 88-89 0.44247604175790656 1105 90-91 0.20753436600950081 1105 92-93 0.3423840262752371 1105 94-95 0.23738589804188592 1105 96-97 0.49292008043987323 1105 98-99 0.40213017957715635 1105 100-101 0.8399050399957027 1106 1 0.11708145109085422 1106 2 0.04598100104244196 1106 3 0.12387803511393258 1106 4 0.11604813181502038 1106 5 0.14088002104530517 1106 6 0.24860267342474174 1106 7 0.21582566531659353 1106 8 0.11882357127825571 1106 9 0.3478775697424581 1106 10-11 0.11038577891126522 1106 12-13 0.1111084190601872 1106 14-15 0.18882991570195884 1106 16-17 0.21252137194752407 1106 18-19 0.35714110690620515 1106 20-21 0.20584561958958858 1106 22-23 0.26547096766035594 1106 24-25 0.2237471681627241 1106 26-27 0.17260971117516277 1106 28-29 0.30319203297786856 1106 30-31 0.14553915935822204 1106 32-33 0.24904253534938192 1106 34-35 0.2311031216287418 1106 36-37 0.3382813209200961 1106 38-39 0.22188097452942657 1106 40-41 0.3484065488153263 1106 42-43 0.32059034418681875 1106 44-45 0.16743472520246172 1106 46-47 0.29514633950834224 1106 48-49 0.31469552421878433 1106 50-51 0.1482976228677444 1106 52-53 0.3054834425368469 1106 54-55 0.39522178670380725 1106 56-57 0.1550322766389911 1106 58-59 0.2974025265031983 1106 60-61 0.2294081576541558 1106 62-63 0.309127206052878 1106 64-65 0.49047711680706385 1106 66-67 0.4652329111571234 1106 68-69 0.48099316888454524 1106 70-71 0.4364490720288927 1106 72-73 0.34162279803991424 1106 74-75 0.5543531383538145 1106 76-77 0.49127410741959565 1106 78-79 0.3814223242566719 1106 80-81 0.7235390756638473 1106 82-83 0.5507999081111059 1106 84-85 0.6392330680455025 1106 86-87 0.6989031132002133 1106 88-89 0.23430972385876458 1106 90-91 0.06679930161031322 1106 92-93 0.4645877458072043 1106 94-95 0.4012961960170678 1106 96-97 0.49926052666581455 1106 98-99 0.6542780471830376 1106 100-101 0.7732971971474036 1107 1 0.18322132806723346 1107 2 -0.034237593434710334 1107 3 0.09386717145287804 1107 4 0.1005931059037195 1107 5 0.10831839913007002 1107 6 -0.05159037676830991 1107 7 0.08704723996022068 1107 8 0.05628411710382153 1107 9 0.23078125017465112 1107 10-11 0.14839758213219056 1107 12-13 0.13620310907882782 1107 14-15 0.13986820276686274 1107 16-17 0.2809569313526623 1107 18-19 0.34249718541817487 1107 20-21 0.29943092134979565 1107 22-23 0.17589714614737062 1107 24-25 0.24826380864895015 1107 26-27 0.2934624351647699 1107 28-29 0.2671951452699446 1107 30-31 0.306236929371579 1107 32-33 0.21525508140983618 1107 34-35 0.22737057321938892 1107 36-37 0.19739321662894582 1107 38-39 0.37561278890762395 1107 40-41 0.5956869633961261 1107 42-43 0.3859851795436313 1107 44-45 0.2875369023388501 1107 46-47 0.15100831229046463 1107 48-49 0.306140391138932 1107 50-51 0.0363303459765163 1107 52-53 -0.1104369591250034 1107 54-55 0.08541659857162642 1107 56-57 -0.002296290385395139 1107 58-59 0.24658371475283047 1107 60-61 0.30029354603003355 1107 62-63 0.3306912062290479 1107 64-65 0.17712472313147032 1107 66-67 0.2704228784193319 1107 68-69 0.18049549576330293 1107 70-71 0.1067571449035043 1107 72-73 0.3800786901079718 1107 74-75 0.3363825876836444 1107 76-77 0.2741770538206403 1107 78-79 0.28363102399389106 1107 80-81 0.29953148578173483 1107 82-83 0.27788162045897735 1107 84-85 0.30131081662515413 1107 86-87 0.27405689272661604 1107 88-89 0.05421885080211197 1107 90-91 0.016723696401630406 1107 92-93 0.2168760879244438 1107 94-95 0.26358644661530306 1107 96-97 0.2928032546191872 1107 98-99 0.42788265396254843 1107 100-101 0.6360095156581984 1108 1 0.026369965341196178 1108 2 -0.041845784303426115 1108 3 -0.026340067394244215 1108 4 -0.030367913455982887 1108 5 -0.08497028400347517 1108 6 0.01902031025458939 1108 7 -0.06147030568166656 1108 8 -0.022725693362609434 1108 9 -0.08641536550467777 1108 10-11 -0.08308625091572708 1108 12-13 -0.11224586488570054 1108 14-15 -0.01823667924632133 1108 16-17 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-0.9721408259045639 2316 88-89 -0.5356100465362914 2316 90-91 -0.5317553910508437 2316 92-93 -0.6894927333683327 2316 94-95 -0.8357481541451541 2316 96-97 -1.0075769735176934 2316 98-99 -1.2698359772161574 2316 100-101 -1.2551501801592941 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 7.0 5 26.0 6 45.0 7 125.0 8 266.0 9 516.0 10 935.0 11 1564.0 12 2332.0 13 3404.0 14 4385.0 15 6107.0 16 8445.0 17 12432.0 18 10927.0 19 5862.0 20 4839.0 21 4804.0 22 5191.0 23 5521.0 24 6377.0 25 7478.0 26 8889.0 27 11077.0 28 13802.0 29 17444.0 30 21335.0 31 27124.0 32 34450.0 33 45359.0 34 63554.0 35 92441.0 36 140036.0 37 218671.0 38 171276.0 39 12402.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.2798100674174 21.362978829657052 15.705344484959543 20.651866617966004 2 14.871078054642892 23.07586768930817 39.7734646193205 22.279589636728442 3 18.3527928678863 28.15532277931258 31.540332713681785 21.951551639119337 4 11.973556221910707 18.88693933589354 39.71208506240117 29.427419379794586 5 11.823936092296456 39.835650579019145 34.91486468921949 13.425548639464905 6 27.99403374892852 40.06166363918622 17.61529439797494 14.329008213910319 7 25.948034613333498 35.30496063243912 22.62131325603201 16.125691498195373 8 25.7906262603757 34.061579055405474 20.751186493826932 19.396608190391902 9 26.540739984300426 17.61209671845876 22.08069911589319 33.76646418134763 10-11 23.50010779274498 27.91311182697872 28.36125113852863 20.225529241747665 12-13 25.617848415549798 26.375646885408578 28.30611695461255 19.700387744429072 14-15 22.62889485101392 27.32391358838438 27.977271719206602 22.069919841395098 16-17 19.758987676033637 30.55193524179885 28.650398033941837 21.038679048225674 18-19 19.53451308263231 29.085788852039634 31.963503762578462 19.416194302749595 20-21 20.87834213566595 27.59639585740221 31.839773477471528 19.685488529460308 22-23 20.664178665701176 27.415408482928 31.849610037825453 20.07080281354537 24-25 20.931229342807274 28.027348988442284 31.760601446674013 19.28082022207643 26-27 20.505178982029072 27.557950854085885 32.667611582021436 19.269258581863607 28-29 19.685669279642003 27.96383922722776 32.71650403912552 19.63398745400472 30-31 20.21555568969102 27.786006472552017 32.20576999173003 19.792667846026934 32-33 20.055351000748363 28.332832189814944 31.914297753518877 19.697519055917816 34-35 20.724135512235176 27.640966022912718 31.972585613223604 19.662312851628503 36-37 20.974629166142233 27.256727005618476 31.8539526380839 19.91469119015539 38-39 20.166013156849576 28.35448600704986 31.88065373676604 19.598847099334524 40-41 20.026573905580108 28.258037162203987 32.172788155630656 19.542600776585253 42-43 19.987311379268547 28.61155025544876 31.029454621417464 20.371683743865233 44-45 20.38628192174405 28.141370910383152 30.90791776332586 20.56442940454694 46-47 21.08687018727403 28.742979157963145 30.32905764697994 19.841093007782884 48-49 20.87352627319835 28.844583746158214 30.027278677296188 20.254611303347247 50-51 20.523920168966576 28.95945782153808 30.167754513640855 20.348867495854492 52-53 20.871259634587137 29.337163098912345 29.587878987935103 20.203698278565415 54-55 20.646766810650504 28.959834955837792 29.611862549158662 20.781535684353038 56-57 20.984779443506625 29.385509551594186 29.09611731654848 20.533593688350713 58-59 21.27280435549522 28.56189436810041 29.44693957967573 20.718361696728635 60-61 20.850778738113142 29.339070589291072 29.827438772173586 19.9827119004222 62-63 20.717230310128976 29.086447336457784 30.159322049633218 20.037000303780026 64-65 20.667786986545845 29.282025571352822 29.69672961502673 20.353457827074603 66-67 20.672756061457846 29.24642450061633 29.068023456720084 21.01279598120574 68-69 20.434570249540844 29.262342557382325 29.513260483753257 20.789826709323574 70-71 21.11885175933696 29.338512409823053 29.18573962504133 20.356896205798662 72-73 21.00989926386121 28.839607784676698 29.474902108655137 20.675590842806955 74-75 20.562480625592837 30.331823956335178 28.628423499653643 20.477271918418335 76-77 20.857181614168212 29.313979283264707 29.0956504965136 20.733188606053478 78-79 20.74350819168153 29.40489220983002 28.9727927439064 20.87880685458205 80-81 21.075905691147483 30.022548798136683 28.361354289480765 20.54019122123507 82-83 20.989844181164543 29.4674513485215 28.902511360178256 20.640193110135705 84-85 20.55045757998366 29.48698640876045 28.553362325777144 21.409193685478748 86-87 20.530185578877987 29.792109571067392 28.6981421482011 20.979562701853517 88-89 20.899559179176876 29.081486608526664 28.595433556331145 21.423520655965316 90-91 20.677068854451033 28.876412085902892 29.020773258059908 21.425745801586167 92-93 20.895206776611943 29.427604461076978 28.850319561979344 20.826869200331735 94-95 20.610513003805263 28.671758229176014 28.98322445682951 21.734504310189212 96-97 20.40675598869876 29.002600440868083 28.94344324777632 21.647200322656843 98-99 20.411387263829084 29.445980536416727 28.272533489297814 21.870098710456375 100-101 20.074393157314915 29.37260945449144 28.537852624803495 22.015144763390147 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2296.0 1 1535.0 2 792.5 3 841.0 4 769.0 5 814.0 6 1332.0 7 2483.0 8 3625.5 9 3917.5 10 3357.0 11 2749.0 12 2600.0 13 2569.0 14 2501.0 15 2377.5 16 2363.0 17 2372.5 18 2325.5 19 2377.0 20 2576.0 21 2799.0 22 3119.0 23 3652.5 24 4078.5 25 4758.5 26 6430.5 27 8036.5 28 8568.0 29 10078.0 30 13024.5 31 15663.0 32 19690.5 33 24530.5 34 29231.5 35 32620.5 36 37046.5 37 41483.5 38 41119.5 39 38957.5 40 36051.0 41 33609.5 42 31267.5 43 30758.0 44 32039.0 45 32026.0 46 30579.5 47 32084.0 48 42322.5 49 40722.5 50 28391.5 51 23292.5 52 21082.0 53 20314.5 54 19496.5 55 18158.0 56 16347.5 57 14468.5 58 13604.0 59 12750.0 60 11200.0 61 9422.5 62 7573.5 63 5924.0 64 4324.5 65 3238.5 66 2383.5 67 1815.5 68 1314.5 69 760.0 70 512.5 71 374.5 72 265.0 73 208.0 74 146.0 75 124.5 76 93.0 77 40.0 78 20.5 79 9.0 80 6.0 81 5.5 82 5.0 83 4.0 84 2.5 85 2.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027541304220008604 2 0.00701426474517073 3 0.009386736644272595 4 0.0 5 0.0056733023674175025 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.157547606743184E-5 18-19 0.003919736181124819 20-21 0.0013409623777532278 22-23 0.0 24-25 0.00587960427168723 26-27 0.005570151415282639 28-29 0.0061890571280918205 30-31 0.02924329493023385 32-33 3.610283324720229E-4 34-35 0.0036102833247202287 36-37 0.015575793772364416 38-39 0.007426868553710184 40-41 0.008355227122923957 42-43 0.008303651646856525 44-45 0.0032492549922482055 46-47 0.001856717138427546 48-49 0.0014956888059555232 50-51 0.003146104040113342 52-53 0.0029913776119110465 54-55 0.0015988397580903872 56-57 7.736321410114776E-4 58-59 0.0011346604734835005 60-61 0.0 62-63 1.5472642820229553E-4 64-65 0.00897413283573314 66-67 8.252076170789095E-4 68-69 0.0012893869016857961 70-71 0.004074462609327116 72-73 0.01428640687067862 74-75 0.007065840221238162 76-77 0.00448706641786657 78-79 0.011965510447644186 80-81 0.0 82-83 0.005982755223822093 84-85 5.157547606743185E-4 86-87 0.0 88-89 0.0011862359495509324 90-91 9.799340452812048E-4 92-93 1.0315095213486368E-4 94-95 6.189057128091821E-4 96-97 2.0630190426972736E-4 98-99 1.5472642820229553E-4 100-101 9.28358569213773E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 969453.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.11580980672332 #Duplication Level Percentage of deduplicated Percentage of total 1 70.27752572833093 24.678522271631735 2 14.214047790625251 9.982755975985443 3 5.462871944910791 5.754995166479162 4 2.802323052360512 3.9362337329475237 5 1.5966241519838016 2.8033375026942045 6 1.0341177055949935 2.1788328400463333 7 0.7041202396155252 1.730802669078226 8 0.49469207918665337 1.3897210372488826 9 0.38850441031666977 1.2278382283578038 >10 2.4848713474267656 17.45672610568984 >50 0.31699288641842405 7.800399465679729 >100 0.20828393786737529 13.620909245398815 >500 0.009427278658654949 2.203735331930914 >1k 0.0047136393293274745 3.067146048309442 >5k 8.838073742489014E-4 2.1680443785220245 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8608 0.8879233959769065 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6575 0.6782175102867287 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5774 0.5955935976267029 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4875 0.5028608916574605 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2887 0.29779679881335147 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2342 0.24157952989985074 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2270 0.23415266134614054 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2169 0.2237344151805193 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1740 0.17948265671466282 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1662 0.17143688244814345 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1564 0.1613280891389268 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1524 0.15720205105353224 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1434 0.14791846536139452 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1410 0.1454428425101578 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1294 0.1334773320625136 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1200 0.12378114256183642 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1181 0.121821274471274 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1094 0.11284714163554087 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1002 0.1033572540391334 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.0630190426972736E-4 0.0 10-11 0.0 0.0 0.0 3.0945285640459106E-4 0.0 12-13 0.0 0.0 0.0 4.1260380853945473E-4 0.0 14-15 0.0 0.0 0.0 6.189057128091821E-4 0.0 16-17 0.0 0.0 0.0 7.220566649440458E-4 0.0 18-19 0.0 0.0 0.0 7.220566649440458E-4 0.0 20-21 0.0 0.0 0.0 7.736321410114777E-4 0.0 22-23 0.0 0.0 0.0 0.0013409623777532278 0.0 24-25 0.0 0.0 0.0 0.0023724718991018643 0.0 26-27 0.0 0.0 0.0 0.0033008304683156374 0.0 28-29 0.0 0.0 0.0 0.00479651927427116 0.0 30-31 0.0 0.0 0.0 0.008303651646856525 0.0 32-33 0.0 0.0 0.0 0.012842293540790528 0.0 34-35 0.0 0.0 0.0 0.01650415234157819 0.0 36-37 0.0 0.0 0.0 0.021403822567984215 0.0 38-39 0.0 0.0 0.0 0.025684587081581056 0.0 40-41 0.0 0.0 0.0 0.03166734230540315 0.0 42-43 0.0 0.0 0.0 0.04048674871293399 0.0 44-45 0.0 0.0 0.0 0.05317431582552223 0.0 46-47 0.0 0.0 0.0 0.09025708311800573 0.0 48-49 0.0 0.0 0.0 0.1657120046046585 0.0 50-51 0.0 0.0 0.0 0.21140787640040312 0.0 52-53 0.0 0.0 0.0 0.25504072915345044 0.0 54-55 0.0 0.0 0.0 0.31352731901391817 0.0 56-57 0.0 0.0 0.0 0.35499400177213336 0.0 58-59 0.0 0.0 0.0 0.41203647830271295 0.0 60-61 0.0 0.0 0.0 0.5078637128360013 0.0 62-63 0.0 0.0 0.0 0.5697027086408522 0.0 64-65 0.0 0.0 0.0 0.647323800122337 0.0 66-67 0.0 0.0 0.0 0.7761593393387818 0.0 68-69 0.0 0.0 0.0 0.9260376727907387 0.0 70-71 0.0 0.0 0.0 1.0501798436850471 0.0 72-73 0.0 0.0 0.0 1.2010381111822852 0.0 74-75 0.0 0.0 0.0 1.3312661882525507 0.0 76-77 0.0 0.0 0.0 1.4246693754106698 0.0 78-79 0.0 0.0 0.0 1.5505135370152034 0.0 80-81 0.0 0.0 0.0 1.722569325176156 0.0 82-83 0.0 0.0 0.0 1.9143269451948677 0.0 84-85 0.0 0.0 0.0 2.1658605419757326 0.0 86-87 0.0 0.0 0.0 2.5902751345346293 0.0 88-89 0.0 0.0 0.0 3.11541663185322 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGAACG 30 0.0039502457 31.66891 36-37 TAGTGCG 65 0.0058408664 29.233597 5 ACCGTCC 70 0.008395423 27.139881 8 ATGGGGA 1100 0.0 26.343456 5 CATGGGG 1810 0.0 25.983175 4 GTACATG 5610 0.0 25.243282 1 GTATGGT 325 0.0 24.857533 1 GAGTACT 5895 0.0 23.687582 12-13 ACATGGG 5700 0.0 23.668955 3 TACATGG 5955 0.0 23.136444 2 GTATAGG 720 0.0 22.440828 1 ATGGGAT 1325 0.0 22.228565 5 GTACTTT 6260 0.0 22.155834 14-15 ATGGGTA 415 0.0 21.74909 5 AGTACTT 6305 0.0 21.657581 12-13 TATAGGG 375 0.0 21.537638 2 TCGAAAT 100 5.389029E-7 21.375412 20-21 GTAAGGT 290 1.05501385E-10 21.295162 4 TAGGACA 515 0.0 21.215643 4 AGGGGTC 485 0.0 20.568996 5 >>END_MODULE