FastQCFastQC Report
Thu 26 May 2016
SRR937065_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937065_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006907
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94750.9410005094810147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65100.6465343869890665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48730.48395730688137034No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42980.42685173506589985No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33040.3281335813535907No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25980.2580178705679869No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19860.19723767934873826No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19080.18949118438942225No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17520.17399819447079024No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15050.1494676270996229No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15020.14916968498580305No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT13610.13516640563627028No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12980.1289096212460535No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12820.12732059663901435No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC12410.12324872108347644No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC12140.12056724205909781No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11810.1172898788070795No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA11760.11679330861737976No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT11720.11639605246561997No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAG502.8165548E-547.5290263
GTATGGT1601.8189894E-1235.6449971
CATGGGT5950.026.3606364
AGGCTTA5050.026.3487996
GAGTACT55200.025.69289812-13
ATGGGTA3950.025.2685975
TGGGTAT2850.025.0115476
GTACATG51450.024.6639821
ATATAGG7550.023.9218962
GTACTTT60350.023.53448314-15
TACATGG54550.022.6536162
CTTACTA6100.022.5901539
GGGTATG2554.8748916E-1022.3621547
CTCCCGC2554.8748916E-1022.3621547
ACATGGG52450.022.2919733
CATATAG2154.391586E-822.1065243
ATGGGAT14050.021.9885185
AGTACTT61550.021.72992312-13
GGTACTC2858.367351E-1121.6799073
ACTTTTT67750.021.03560816-17
Produced by FastQC (version 0.11.5)