FastQCFastQC Report
Thu 26 May 2016
SRR937065_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937065_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006907
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85440.8485391401589224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66440.6598424680730196No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT58720.5831720307833792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48060.4773032663393938No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29160.2895997346328906No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24240.24073722796643582No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23070.2291174855274618No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21720.21571009040556874No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18210.1808508630886467No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16830.16714552585293377No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15710.1560223536036595No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15390.15284430438958116No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15200.15095733766872213No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14290.1419197602161868No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13840.13745062850888912No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG12370.12285146493171664No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA11850.11768713495883928No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT11270.11192692075832228No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC10870.10795435924072432No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC10190.10120100466080781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAG2400.031.6634223
TAGACGC650.00584665929.2277764
CGTTTAA700.00837699227.1522121
GTTCTAG1658.380084E-825.9180181
GGACAAT5050.025.395966
CATGGGG18800.025.0107354
TGTATAC950.001227182124.9974382
GTACATG56850.024.4895291
ATGGGTA3300.024.469625
GTATAGG4900.024.2430441
CTCCCGC3150.024.126917
GAGTACT61150.023.99999212-13
CGTATAG2252.8012437E-923.2302251
ACATGGG57000.022.581023
TAGGACA5950.022.350654
TACATGG60900.022.3047752
GTACTTT65950.022.28922514-15
ACATATA4050.022.2816682
AGTACTT66100.021.59196312-13
GTGGTAC4000.021.3823681
Produced by FastQC (version 0.11.5)