##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937065_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1006907 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22813030399034 33.0 31.0 34.0 31.0 34.0 2 32.35159751595728 34.0 31.0 34.0 31.0 34.0 3 32.44330012602951 34.0 31.0 34.0 31.0 34.0 4 36.09406529103482 37.0 35.0 37.0 35.0 37.0 5 36.01231593384493 37.0 35.0 37.0 35.0 37.0 6 36.074114093953064 37.0 35.0 37.0 35.0 37.0 7 36.01915171907634 37.0 35.0 37.0 35.0 37.0 8 35.990371504021724 37.0 35.0 37.0 35.0 37.0 9 37.659887159390095 39.0 37.0 39.0 35.0 39.0 10-11 37.582511592431075 39.0 37.0 39.0 35.0 39.0 12-13 37.50805188562598 39.0 37.0 39.0 35.0 39.0 14-15 38.66680736155375 40.0 38.0 41.0 34.0 41.0 16-17 38.59902652380011 40.0 38.0 41.0 34.0 41.0 18-19 38.793197385657265 40.0 38.0 41.0 35.0 41.0 20-21 38.83550665553025 40.0 38.0 41.0 35.0 41.0 22-23 38.74419186677618 40.0 38.0 41.0 35.0 41.0 24-25 38.62433918922006 40.0 38.0 41.0 34.0 41.0 26-27 38.427649723360744 40.0 38.0 41.0 34.0 41.0 28-29 38.34898059105757 40.0 38.0 41.0 34.0 41.0 30-31 38.30244352258947 40.0 38.0 41.0 34.0 41.0 32-33 38.084950248632694 40.0 37.0 41.0 34.0 41.0 34-35 37.91747698645456 40.0 37.0 41.0 33.0 41.0 36-37 37.72768587367056 40.0 37.0 41.0 33.0 41.0 38-39 37.55291749883554 40.0 36.5 41.0 33.0 41.0 40-41 37.378825949169084 40.0 36.0 41.0 33.0 41.0 42-43 37.101154823633166 40.0 36.0 41.0 32.0 41.0 44-45 36.78973877428601 40.0 36.0 41.0 31.0 41.0 46-47 36.348101661821794 39.0 35.5 41.0 30.5 41.0 48-49 35.91906551449141 39.0 35.0 41.0 29.5 41.0 50-51 35.617603214596784 39.0 35.0 40.0 28.0 41.0 52-53 35.398183248303965 39.0 35.0 40.0 27.5 41.0 54-55 35.12886195050784 38.5 35.0 40.0 27.0 41.0 56-57 34.85464596035185 38.0 34.5 40.0 26.0 41.0 58-59 34.52827768602265 38.0 34.0 40.0 26.0 41.0 60-61 34.192946319769355 38.0 34.0 40.0 25.0 41.0 62-63 33.84585269543265 37.0 33.0 40.0 24.0 41.0 64-65 33.46626550416275 36.5 33.0 39.0 23.0 40.5 66-67 33.06490966891679 36.0 33.0 39.0 22.0 40.0 68-69 32.76073559921621 36.0 32.0 39.0 22.0 40.0 70-71 32.29187799866323 35.0 32.0 38.0 20.5 40.0 72-73 31.78945423956731 35.0 31.0 37.5 18.5 39.5 74-75 31.272740680122396 35.0 31.0 37.0 17.5 39.0 76-77 29.838296386855987 33.5 29.5 35.5 14.0 37.5 78-79 30.57149170678126 34.0 30.5 36.0 13.5 38.0 80-81 30.31627598179375 34.0 30.0 35.5 8.0 37.0 82-83 29.99576872541357 34.0 30.0 35.0 7.0 37.0 84-85 29.633422947700232 34.0 30.0 35.0 3.5 36.0 86-87 29.308856726589447 34.0 29.5 35.0 2.0 36.0 88-89 28.908815312635625 34.0 29.0 35.0 2.0 35.5 90-91 28.45743003077742 34.0 29.0 35.0 2.0 35.0 92-93 27.992224703969683 33.0 28.5 35.0 2.0 35.0 94-95 27.41360175269414 33.0 27.0 35.0 2.0 35.0 96-97 26.755735633976126 33.0 25.5 34.5 2.0 35.0 98-99 25.994531769071024 32.5 24.5 34.0 2.0 35.0 100-101 22.483868420817416 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2110504599952776 1101 2 0.2021513339804315 1101 3 0.09463104809053391 1101 4 0.18612742309237973 1101 5 0.2850051369630222 1101 6 0.22755124444290686 1101 7 0.2715227182847215 1101 8 0.25784035849741826 1101 9 0.3271280724975014 1101 10-11 0.3702660280818719 1101 12-13 0.2799841814369657 1101 14-15 0.4141439123224302 1101 16-17 0.40250357766171163 1101 18-19 0.31008733122970966 1101 20-21 0.2927558313105507 1101 22-23 0.3034716975269447 1101 24-25 0.4991214296405886 1101 26-27 0.3585721230475727 1101 28-29 0.33995574413001606 1101 30-31 0.46995841432127605 1101 32-33 0.45189183544407996 1101 34-35 0.4295365664870303 1101 36-37 0.34076903099393263 1101 38-39 0.3464170700677798 1101 40-41 0.2738368494150265 1101 42-43 0.2950927852597971 1101 44-45 0.09928129190046775 1101 46-47 0.21243454425157182 1101 48-49 0.09472537772391831 1101 50-51 0.045499503902497906 1101 52-53 0.12919206987599807 1101 54-55 -0.04384172139830156 1101 56-57 -0.02413186779941867 1101 58-59 0.017969040662720204 1101 60-61 0.2287186741989018 1101 62-63 0.0014673955261272909 1101 64-65 0.001790597378935388 1101 66-67 -0.2714670404430919 1101 68-69 -0.33067459802931154 1101 70-71 -0.14100318638343623 1101 72-73 -0.33368762737485724 1101 74-75 -0.02111492295949091 1101 76-77 -0.1715950032115856 1101 78-79 -0.38956374134449234 1101 80-81 -0.04133346073067301 1101 82-83 0.061014849278148375 1101 84-85 -0.10896927685683622 1101 86-87 -0.3614152897690204 1101 88-89 -0.2874777401187636 1101 90-91 0.03752402162016111 1101 92-93 -0.03434551937582597 1101 94-95 -0.12890926677672 1101 96-97 -0.08911842154459393 1101 98-99 -0.04860911640175658 1101 100-101 0.6739265405749606 1102 1 0.28480328552227263 1102 2 0.23783996153154163 1102 3 0.3088704525207575 1102 4 0.1775613514447798 1102 5 0.2676226701246307 1102 6 0.09783108113182948 1102 7 0.09042114450060978 1102 8 0.21499247761137497 1102 9 0.2375573022769899 1102 10-11 0.2832347902069188 1102 12-13 0.21448201989316118 1102 14-15 0.40964434663378313 1102 16-17 0.4916850947725777 1102 18-19 0.31115746437680514 1102 20-21 0.30904688750067777 1102 22-23 0.23141731724042103 1102 24-25 0.25856300788046127 1102 26-27 0.1831328678101798 1102 28-29 0.1557489067927591 1102 30-31 0.23258661126582325 1102 32-33 0.2706600934175043 1102 34-35 0.25620002901234784 1102 36-37 0.20746423454452412 1102 38-39 0.30754307542970594 1102 40-41 0.25580854783522966 1102 42-43 0.3371508953671096 1102 44-45 0.3828427951434108 1102 46-47 0.33891927576704717 1102 48-49 0.4241361411356337 1102 50-51 0.3833787127799724 1102 52-53 0.22448899114674248 1102 54-55 0.23980614346703533 1102 56-57 0.13626419411060198 1102 58-59 0.18950450024422594 1102 60-61 0.16079338075713423 1102 62-63 0.39000833469000185 1102 64-65 0.286781448053425 1102 66-67 0.32461464513551874 1102 68-69 0.1250356574759266 1102 70-71 0.11306269376665767 1102 72-73 0.25279039546966686 1102 74-75 0.5302201776580588 1102 76-77 0.25810162968643624 1102 78-79 0.2916479747986216 1102 80-81 0.3722485940751632 1102 82-83 0.29866451146652295 1102 84-85 0.1635654743090491 1102 86-87 0.15851547073894068 1102 88-89 0.26105123357974236 1102 90-91 0.526096751874018 1102 92-93 0.5802964053118345 1102 94-95 0.4520253412040951 1102 96-97 0.5917778869341142 1102 98-99 0.7604353235368606 1102 100-101 1.26805319928134 1103 1 0.14717130965500047 1103 2 0.17301916141661877 1103 3 0.11546745029422567 1103 4 0.17075606522772802 1103 5 0.029994285437425106 1103 6 0.10384630773528869 1103 7 0.024576858068087404 1103 8 0.043782968166574676 1103 9 0.12375280611955475 1103 10-11 0.20626682353307046 1103 12-13 0.16572277260450363 1103 14-15 0.1449205083805296 1103 16-17 0.3218671676017095 1103 18-19 0.1594929524181694 1103 20-21 0.08585504666177712 1103 22-23 0.3471969624318305 1103 24-25 0.10673689281906462 1103 26-27 0.21193413190555788 1103 28-29 0.29977318316997525 1103 30-31 0.3304193340985435 1103 32-33 0.3699382662250841 1103 34-35 0.2817692310521309 1103 36-37 0.22561764975676368 1103 38-39 0.27411816700480074 1103 40-41 0.42563545295625005 1103 42-43 0.4144779800471383 1103 44-45 0.40418338266047016 1103 46-47 0.35029433861270576 1103 48-49 0.3102239095763366 1103 50-51 0.32822716498137083 1103 52-53 0.3505214554145013 1103 54-55 0.24345119122973813 1103 56-57 0.25749345504573995 1103 58-59 0.1385007654169641 1103 60-61 0.1827175350342216 1103 62-63 0.07768340471800883 1103 64-65 0.15140293767492352 1103 66-67 0.1650674929413114 1103 68-69 0.18020516117484675 1103 70-71 0.18717567229140286 1103 72-73 0.33143723882238874 1103 74-75 0.39219317198399395 1103 76-77 0.14361480931726334 1103 78-79 0.330199292761705 1103 80-81 0.48405100734816386 1103 82-83 0.4912864320296215 1103 84-85 0.28789212804746356 1103 86-87 0.3248319973495839 1103 88-89 0.37502631079011195 1103 90-91 0.20526970310578818 1103 92-93 0.2764107484849987 1103 94-95 0.23373546114950727 1103 96-97 0.05205943545095337 1103 98-99 0.19640458303553388 1103 100-101 0.4500990945625283 1104 1 0.13865257571093537 1104 2 0.1691207284066607 1104 3 0.06823443756026393 1104 4 0.03990755016371139 1104 5 0.09219610378291065 1104 6 0.1026743542949049 1104 7 0.0868081050819427 1104 8 0.15114740587127784 1104 9 0.08851457239360627 1104 10-11 0.11911728435138969 1104 12-13 0.06715491009524754 1104 14-15 0.04921674774293194 1104 16-17 0.22173439237441528 1104 18-19 0.3584177673637754 1104 20-21 0.22368508052205271 1104 22-23 0.26631659516204564 1104 24-25 0.17947503999009484 1104 26-27 0.07327317093836427 1104 28-29 0.01664688917411894 1104 30-31 0.14572760805521767 1104 32-33 0.15382451230414773 1104 34-35 0.1332375484939874 1104 36-37 0.15630274915641706 1104 38-39 0.04982410882605848 1104 40-41 0.08480473358111595 1104 42-43 0.18762189662205486 1104 44-45 -0.09543837239606745 1104 46-47 0.030701596760984273 1104 48-49 -0.2082295965898311 1104 50-51 -0.1797488696959917 1104 52-53 -0.28841301905872996 1104 54-55 -0.2242430552924901 1104 56-57 -0.16198364945027066 1104 58-59 -0.11031776010288752 1104 60-61 0.026634802391946266 1104 62-63 -0.019242511206009283 1104 64-65 -0.1090762649120336 1104 66-67 -0.16406879295301025 1104 68-69 -0.052013864286145406 1104 70-71 -0.04886042456699613 1104 72-73 -0.07180995506752197 1104 74-75 -0.018234616988220154 1104 76-77 -0.025328251402733315 1104 78-79 -0.0015725900498750889 1104 80-81 0.27972689730571787 1104 82-83 0.18004623870058367 1104 84-85 0.20868919487477555 1104 86-87 0.09282416586160025 1104 88-89 0.1373006621476378 1104 90-91 0.19573221790667716 1104 92-93 0.0904215643861761 1104 94-95 0.2662454842077331 1104 96-97 0.3404880830478447 1104 98-99 0.25912919301912396 1104 100-101 0.3890216244585929 1105 1 0.2214364753835838 1105 2 0.2104532720214678 1105 3 0.17811821416449902 1105 4 0.051194732251758523 1105 5 0.09436487419825568 1105 6 0.1605111875708971 1105 7 0.2240542099844589 1105 8 0.13426362572040063 1105 9 0.246329416501041 1105 10-11 0.30075668326682603 1105 12-13 0.16618064806237953 1105 14-15 0.3052885955778848 1105 16-17 0.26874244679405734 1105 18-19 0.27587149257719545 1105 20-21 0.1708188633329044 1105 22-23 0.2804588225229665 1105 24-25 0.1913545109732624 1105 26-27 0.3153916499972311 1105 28-29 0.3662879691969607 1105 30-31 0.3208039494831638 1105 32-33 0.22486991993722683 1105 34-35 0.17292888440690746 1105 36-37 0.22159727988272948 1105 38-39 0.30066377586748416 1105 40-41 0.14896699945852987 1105 42-43 0.16223327353413453 1105 44-45 -0.027794192609796653 1105 46-47 1.9613337975954437E-5 1105 48-49 -0.24845115519629246 1105 50-51 -0.02169019408233197 1105 52-53 0.044682984941879056 1105 54-55 0.08319478097332222 1105 56-57 0.13559807400401525 1105 58-59 0.1373281575370413 1105 60-61 -0.04834205578146111 1105 62-63 0.12027698999452241 1105 64-65 -0.03307620107251097 1105 66-67 -0.10998164491270046 1105 68-69 -0.0737370348405193 1105 70-71 -0.3388115518665522 1105 72-73 -0.028609218785042856 1105 74-75 0.3737888121162207 1105 76-77 0.2784982185909186 1105 78-79 0.2861539072529027 1105 80-81 0.24824514654230612 1105 82-83 0.26709497308206664 1105 84-85 0.44803686136292953 1105 86-87 0.2679102605497974 1105 88-89 0.207678414783679 1105 90-91 0.21897246375977275 1105 92-93 0.4757764234604309 1105 94-95 0.3047619955906775 1105 96-97 0.2858662790138986 1105 98-99 0.4680475741907202 1105 100-101 0.6890319097148563 1106 1 0.1156547337640319 1106 2 0.11218168011357221 1106 3 0.1225900561074198 1106 4 0.03822621228080436 1106 5 0.09735837947464887 1106 6 0.2060133776521127 1106 7 0.0997545901323349 1106 8 0.09912860862375794 1106 9 0.13843857298766693 1106 10-11 0.06347407635138325 1106 12-13 0.16558376676714914 1106 14-15 0.30009454546532766 1106 16-17 0.24992476309383704 1106 18-19 0.10997534264352993 1106 20-21 0.26801042765323757 1106 22-23 0.1976888565706716 1106 24-25 0.12116818574210697 1106 26-27 0.24802413307801174 1106 28-29 0.23235135507889737 1106 30-31 0.2037965630771339 1106 32-33 0.18482246059522112 1106 34-35 0.17992813648553607 1106 36-37 0.2860545455476782 1106 38-39 0.23415154617009648 1106 40-41 0.5112452844438735 1106 42-43 0.4227983454237645 1106 44-45 0.18554674125466875 1106 46-47 0.06429626781831388 1106 48-49 0.06785635149587677 1106 50-51 0.22438003409738627 1106 52-53 0.20908101741732565 1106 54-55 -0.04834293446351978 1106 56-57 0.18701038977606288 1106 58-59 0.08139601274965003 1106 60-61 -0.024955409544638485 1106 62-63 0.09343325460947227 1106 64-65 -0.25807406285445467 1106 66-67 -0.17755524965718195 1106 68-69 -0.12485136490496274 1106 70-71 -0.24346840881339915 1106 72-73 -0.19510088519390933 1106 74-75 -0.0993110399372874 1106 76-77 0.021382046929311116 1106 78-79 0.2670568903855468 1106 80-81 0.3460590687985139 1106 82-83 0.3561572143349352 1106 84-85 0.28914451290799903 1106 86-87 0.20208362544990166 1106 88-89 0.2671973066226023 1106 90-91 0.14573212623067633 1106 92-93 0.19974703069615884 1106 94-95 0.30618459300697864 1106 96-97 0.2127146547870673 1106 98-99 0.3802386926698347 1106 100-101 0.7754309321397592 1107 1 0.16711905543213135 1107 2 0.12312766270995468 1107 3 0.17576899708866023 1107 4 0.04878238330143603 1107 5 0.1445733872203192 1107 6 -0.04850600030422214 1107 7 0.0472449482717181 1107 8 0.17614673394265878 1107 9 0.17397762079934864 1107 10-11 0.12339657371968826 1107 12-13 0.09195534729679622 1107 14-15 0.10284023478367033 1107 16-17 0.16428987128033867 1107 18-19 0.07630674683859695 1107 20-21 0.11148912822002899 1107 22-23 0.15112384124022782 1107 24-25 0.176514074563066 1107 26-27 0.14338041074520902 1107 28-29 0.1455980534024306 1107 30-31 0.01043209385064614 1107 32-33 -0.03938676892932591 1107 34-35 -0.07759573257823149 1107 36-37 0.05164494000205622 1107 38-39 0.024920642570307905 1107 40-41 0.009782111984428354 1107 42-43 -0.017331561122588823 1107 44-45 -0.38025188980797964 1107 46-47 -0.5057769597571777 1107 48-49 -0.3295413804259226 1107 50-51 -0.41991138683017226 1107 52-53 -0.21101266607222868 1107 54-55 -0.025153742541078827 1107 56-57 -0.27195596599135996 1107 58-59 -0.279390229843699 1107 60-61 -0.23446993784045134 1107 62-63 -0.07151542173200198 1107 64-65 -0.13852510426733744 1107 66-67 -0.14125726444979847 1107 68-69 -0.16013344966139442 1107 70-71 -0.08241922727980011 1107 72-73 -0.36134265111389396 1107 74-75 -0.3375700347934334 1107 76-77 -0.3094084707202356 1107 78-79 -0.18346232208474333 1107 80-81 -0.23760975684814412 1107 82-83 -0.18729758269182994 1107 84-85 -0.1288622076642163 1107 86-87 -0.3568707785475027 1107 88-89 -0.18735195075876732 1107 90-91 -0.16941260510732192 1107 92-93 -0.07158848766589543 1107 94-95 0.014447403235855916 1107 96-97 0.15289098936307965 1107 98-99 -0.011027321552766978 1107 100-101 0.5537807604479852 1108 1 0.009428970693150518 1108 2 -0.040015771130505584 1108 3 -0.024508427486530593 1108 4 -0.031356862494959614 1108 5 -0.006010360323323027 1108 6 0.015107810460207816 1108 7 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-0.9076031088502106 2316 84-85 -1.0366511819327364 2316 86-87 -0.9564709769145594 2316 88-89 -1.2136933568130495 2316 90-91 -1.3426857125680733 2316 92-93 -1.5207252331170018 2316 94-95 -1.428020528276388 2316 96-97 -0.9721175741629473 2316 98-99 -1.1338881016260842 2316 100-101 -1.439624046374206 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 2.0 5 31.0 6 54.0 7 119.0 8 297.0 9 555.0 10 1015.0 11 1680.0 12 2569.0 13 3699.0 14 4537.0 15 6543.0 16 8935.0 17 12781.0 18 11225.0 19 6202.0 20 5157.0 21 5131.0 22 5557.0 23 6233.0 24 6958.0 25 8252.0 26 9949.0 27 12501.0 28 15468.0 29 19062.0 30 23737.0 31 29869.0 32 38237.0 33 50052.0 34 68760.0 35 100370.0 36 150844.0 37 225566.0 38 156238.0 39 8720.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.21682462100892 21.377083705867157 15.722218910810435 20.683872762313484 2 14.857633219312778 23.02877529210318 39.64796650099917 22.46562498758487 3 18.273178521842244 28.266405270820794 31.480322811951904 21.980093395385055 4 11.967973113623543 18.899319100927197 39.518464520947376 29.614243264501876 5 11.851751802055007 39.79110264513272 34.9127922566754 13.44435329613688 6 28.03953095966162 40.03031064437928 17.66180987916461 14.268348516794498 7 25.950261543518916 35.255291700226536 22.616785860064535 16.177660896190016 8 25.765835375064427 34.01148268906662 20.825855813893437 19.396826121975515 9 26.408794456687655 17.609372067132316 22.139879849876902 33.84195362630312 10-11 23.473855472307946 27.895972164419046 28.405374023236714 20.224798340036294 12-13 25.510002413331122 26.34861014969605 28.386534208223797 19.75485322874903 14-15 22.65730598754403 27.208371776142187 28.03545908410608 22.098863152207702 16-17 19.8129519409439 30.492140783607624 28.64936880963187 21.045538465816605 18-19 19.645626418023177 29.007397431829368 31.897594953240304 19.44938119690715 20-21 20.920291826977508 27.5919209294288 31.77312895645835 19.71465828713534 22-23 20.72758205273105 27.36420219628298 31.869492284605062 20.0387234663809 24-25 20.980474051898685 27.977425248699205 31.711129000778215 19.33097169862389 26-27 20.490122172992827 27.517030821839576 32.59936177249663 19.39348523267097 28-29 19.723508482698175 27.910398474529558 32.64561365176867 19.720479391003597 30-31 20.27225930630637 27.767559101414186 32.11240566070529 19.847775931574155 32-33 20.130097466332227 28.282766083285622 31.868267749107247 19.718868701274904 34-35 20.77053789476089 27.51604666568015 31.961491974929164 19.751923464629805 36-37 20.974899707092067 27.27028479312198 31.829428059765537 19.92538744002042 38-39 20.21249967718802 28.269936371275495 31.874355617623745 19.64320833391274 40-41 20.098173827761297 28.16188501694579 32.10333825774975 19.63660289754317 42-43 20.038643092162435 28.54310278903613 30.9841921845811 20.434061934220342 44-45 20.391356897906164 28.10358851413288 30.877528907833597 20.62752568012736 46-47 21.09966734680399 28.62679628504987 30.235737351456866 20.03779901668927 48-49 20.916427351590304 28.856109523953773 29.922785195455404 20.304677929000515 50-51 20.579902455691133 28.88065397693441 30.136533228978834 20.402910338395618 52-53 20.850535352321515 29.336274353043528 29.555758376890818 20.257431917744142 54-55 20.695807713584532 28.899970502734707 29.548761363151062 20.8554604205297 56-57 21.072314805756662 29.31202038796526 29.047338356498393 20.56832644977969 58-59 21.34015688677526 28.539499028954584 29.38813959019947 20.73220449407069 60-61 20.85011356763258 29.271349672108844 29.80055398360693 20.077982776651645 62-63 20.78565143030811 29.08478555806946 30.079326927851923 20.050236083770507 64-65 20.719093223107993 29.261143283341955 29.64533963911265 20.374423854437403 66-67 20.702571390831345 29.306565682147053 28.919133924660862 21.071729002360744 68-69 20.541154411100177 29.11005330205591 29.5504134956396 20.798378791204318 70-71 21.106627589515114 29.272457858152322 29.245543486573105 20.375371065759456 72-73 21.066344756765023 28.683334210607335 29.498255548923584 20.752065483704058 74-75 20.65041756587252 30.20532184203705 28.562965596624117 20.581294995466315 76-77 20.895623925546253 29.192318655049572 29.12900595586286 20.78305146354132 78-79 20.81724206253634 29.234029853414462 28.975460491326483 20.973267592722717 80-81 21.098455486293638 29.95124078036601 28.407471944454514 20.542831788885838 82-83 21.059184492403535 29.40531434746679 28.860013667281166 20.675487492848514 84-85 20.580501271578473 29.381880267515676 28.615506553061397 21.422111907844453 86-87 20.567631224931556 29.63276733754209 28.739403407694255 21.060198029832097 88-89 20.86887951804836 29.090302371820208 28.62059616289902 21.420221947232413 90-91 20.709584928371626 28.760942637217155 29.0725927191271 21.456879715284114 92-93 20.98635772040403 29.334799209261238 28.812854023685418 20.865989046649318 94-95 20.628181464713954 28.60280472913821 28.945984557652572 21.823029248495267 96-97 20.443230133451717 28.873968224646205 28.953173215106847 21.729628426795223 98-99 20.495535758623088 29.325795270585665 28.293408416013666 21.885260554777584 100-101 20.18081158640169 29.213197478013058 28.568945714315507 22.037045221269743 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2300.0 1 1526.0 2 829.5 3 877.0 4 769.0 5 808.0 6 1310.5 7 2492.5 8 3665.5 9 3999.0 10 3372.0 11 2731.5 12 2644.0 13 2596.5 14 2509.5 15 2389.0 16 2349.5 17 2342.0 18 2325.0 19 2444.5 20 2631.0 21 2902.0 22 3267.0 23 3755.0 24 4200.5 25 4940.0 26 6660.0 27 8290.0 28 8852.5 29 10461.0 30 13635.0 31 16221.0 32 20080.0 33 25258.0 34 30247.0 35 33798.0 36 38480.5 37 43054.5 38 42911.5 39 40545.5 40 37272.0 41 34804.5 42 32397.5 43 31998.5 44 33244.5 45 33266.0 46 31778.0 47 33429.5 48 44142.5 49 42287.5 50 29562.0 51 24399.0 52 22070.0 53 21204.5 54 20440.5 55 18971.0 56 17079.0 57 15230.0 58 14270.5 59 13531.0 60 11830.0 61 9746.0 62 7919.5 63 6268.5 64 4606.0 65 3408.5 66 2500.5 67 1864.0 68 1325.5 69 805.5 70 565.5 71 402.0 72 283.5 73 220.5 74 158.0 75 119.0 76 85.0 77 44.5 78 18.5 79 14.5 80 12.5 81 6.0 82 1.5 83 1.5 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0285031288887653 2 0.007051296693736362 3 4.965701896997439E-4 4 2.9794211381984633E-4 5 9.931403793994877E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.01370533723571293 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.4759913278982073E-4 20-21 8.938263414595389E-4 22-23 0.0023835369105587706 24-25 0.01176871349588393 26-27 0.005561586124637132 28-29 4.4691317072976947E-4 30-31 0.0035753053658381558 32-33 0.004618102764207618 34-35 0.0 36-37 0.01052728802163457 38-39 0.012811510894253392 40-41 0.0019366237398290012 42-43 0.0 44-45 0.0 46-47 2.4828509484987193E-4 48-49 0.004022218536567926 50-51 0.008193408130045774 52-53 0.0 54-55 0.003376677289958258 56-57 0.0038235904606880276 58-59 2.4828509484987193E-4 60-61 0.0017876526829190779 62-63 0.006058156314336875 64-65 0.0 66-67 0.0019862807587989755 68-69 3.9725615175979507E-4 70-71 9.931403793994877E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0020359377777689498 80-81 0.0020359377777689498 82-83 0.012215626666613699 84-85 0.008689978319745518 86-87 0.0038235904606880276 88-89 9.931403793994877E-5 90-91 8.441693224895645E-4 92-93 4.9657018969974384E-5 94-95 4.9657018969974384E-5 96-97 0.0025821649864386682 98-99 0.001688338644979129 100-101 2.4828509484987193E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1006907.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.78485410922309 #Duplication Level Percentage of deduplicated Percentage of total 1 69.92967300384396 24.324934733443886 2 14.362753756470505 9.99212588051045 3 5.541505678342434 5.782813996997187 4 2.8238851012835986 3.9291372507743465 5 1.630313738555434 2.8355112773956423 6 1.047107186823242 2.1854082438219287 7 0.6856393795856356 1.6694906053297196 8 0.5003638973413314 1.3924068136432393 9 0.3870524289112805 1.2117206045069402 >10 2.542280518240324 17.60881865484251 >50 0.3230739876328077 7.854606788184791 >100 0.20918181680288578 13.415376267120527 >500 0.011446337444328455 2.593011647284442 >1k 0.0048646934138395935 3.108329163522122 >5k 8.584753083246341E-4 2.096308072622278 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8544 0.8485391401589224 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6644 0.6598424680730196 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5872 0.5831720307833792 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4806 0.4773032663393938 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2916 0.2895997346328906 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2424 0.24073722796643582 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2307 0.2291174855274618 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2172 0.21571009040556874 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1821 0.1808508630886467 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1683 0.16714552585293377 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1571 0.1560223536036595 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1539 0.15284430438958116 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1520 0.15095733766872213 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1429 0.1419197602161868 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1384 0.13745062850888912 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1237 0.12285146493171664 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1185 0.11768713495883928 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1127 0.11192692075832228 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1087 0.10795435924072432 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1019 0.10120100466080781 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.931403793994877E-5 0.0 12-13 0.0 0.0 0.0 9.931403793994877E-5 0.0 14-15 0.0 0.0 0.0 9.931403793994877E-5 0.0 16-17 0.0 0.0 0.0 9.931403793994877E-5 0.0 18-19 0.0 0.0 0.0 9.931403793994877E-5 0.0 20-21 0.0 0.0 0.0 2.4828509484987193E-4 0.0 22-23 0.0 0.0 0.0 6.455412466096671E-4 0.0 24-25 0.0 0.0 0.0 8.441693224895645E-4 0.0 26-27 0.0 0.0 0.0 0.0019366237398290012 0.0 28-29 0.0 0.0 0.0 0.0038235904606880276 0.0 30-31 0.0 0.0 0.0 0.0073492388075562095 0.0 32-33 0.0 0.0 0.0 0.012215626666613699 0.0 34-35 0.0 0.0 0.0 0.015542646937601982 0.0 36-37 0.0 0.0 0.0 0.020309720758719525 0.0 38-39 0.0 0.0 0.0 0.024828509484987195 0.0 40-41 0.0 0.0 0.0 0.02989352541992458 0.0 42-43 0.0 0.0 0.0 0.03734207826542074 0.0 44-45 0.0 0.0 0.0 0.049756333007914336 0.0 46-47 0.0 0.0 0.0 0.08173545322457784 0.0 48-49 0.0 0.0 0.0 0.14782894547361375 0.0 50-51 0.0 0.0 0.0 0.1943079152295098 0.0 52-53 0.0 0.0 0.0 0.23701295154368773 0.0 54-55 0.0 0.0 0.0 0.28920247848113084 0.0 56-57 0.0 0.0 0.0 0.33319859728852813 0.0 58-59 0.0 0.0 0.0 0.385835037396701 0.0 60-61 0.0 0.0 0.0 0.47328104780282587 0.0 62-63 0.0 0.0 0.0 0.5372392882361529 0.0 64-65 0.0 0.0 0.0 0.6104833912168651 0.0 66-67 0.0 0.0 0.0 0.7409820370699578 0.0 68-69 0.0 0.0 0.0 0.8901020650367908 0.0 70-71 0.0 0.0 0.0 1.0124073027598377 0.0 72-73 0.0 0.0 0.0 1.161775615821521 0.0 74-75 0.0 0.0 0.0 1.2877554729483458 0.0 76-77 0.0 0.0 0.0 1.3811106686118977 0.0 78-79 0.0 0.0 0.0 1.5067925836249028 0.0 80-81 0.0 0.0 0.0 1.68699790546694 0.0 82-83 0.0 0.0 0.0 1.8820506759809992 0.0 84-85 0.0 0.0 0.0 2.126611494408123 0.0 86-87 0.0 0.0 0.0 2.529826488444315 0.0 88-89 0.0 0.0 0.0 3.0373708793364234 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATAG 240 0.0 31.663422 3 TAGACGC 65 0.005846659 29.227776 4 CGTTTAA 70 0.008376992 27.152212 1 GTTCTAG 165 8.380084E-8 25.918018 1 GGACAAT 505 0.0 25.39596 6 CATGGGG 1880 0.0 25.010735 4 TGTATAC 95 0.0012271821 24.997438 2 GTACATG 5685 0.0 24.489529 1 ATGGGTA 330 0.0 24.46962 5 GTATAGG 490 0.0 24.243044 1 CTCCCGC 315 0.0 24.12691 7 GAGTACT 6115 0.0 23.999992 12-13 CGTATAG 225 2.8012437E-9 23.230225 1 ACATGGG 5700 0.0 22.58102 3 TAGGACA 595 0.0 22.35065 4 TACATGG 6090 0.0 22.304775 2 GTACTTT 6595 0.0 22.289225 14-15 ACATATA 405 0.0 22.281668 2 AGTACTT 6610 0.0 21.591963 12-13 GTGGTAC 400 0.0 21.382368 1 >>END_MODULE