FastQCFastQC Report
Thu 26 May 2016
SRR937064_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937064_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019755
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94930.9309098754112507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66590.6529999852905845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47440.4652097807806777No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44780.43912508396624683No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34180.33517854778843936No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25990.2548651391755863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20420.2002441762972479No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19980.19592941441816908No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18060.17710136258218886No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15910.15601786703669018No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14400.1412103887698516No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT14280.1400336355301028No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13610.13346342994150556No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12890.12640291050301297No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12750.1250300317233061No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC12740.12493196895332702No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA12720.1247358434133689No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12210.11973464214443666No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC12200.1196365793744576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGG500.001610870238.0233085
GGTATGG1657.6397555E-1131.6798698
GGGTATG2600.031.070647
GTACATG56150.027.2549081
ATGGGTA4850.025.4795365
GAGTACT58100.025.35480112-13
ACATGGG56800.025.2707733
TACATGG58950.025.1553692
CATGGGT7300.024.7424074
CATGGGG18700.024.401184
GTTCTAG1951.5599653E-824.3727171
CTGTACG1000.001647155723.7657344
GTACTTT62500.023.42005714-15
GGTAAGG1252.2463134E-422.8139843
ATGGGGA10850.022.7789385
GGTTCGT1050.002190738522.6284779
AGTACTT63000.021.7233412-13
GTATCAA136350.020.9835971
ACTTTTT72550.020.66809816-17
CATGGGA33700.020.4512234
Produced by FastQC (version 0.11.5)