FastQCFastQC Report
Thu 26 May 2016
SRR937064_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937064_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019755
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88840.871189648494001No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68500.6717299743565857No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59220.5807277238160147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49660.4869797157160298No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28750.28193046368980784No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24670.24192085353834988No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24370.23897897043897798No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22590.2215237973827047No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17650.17308078901304727No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16290.1597442522958946No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC16230.15915587567602021No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15860.15552755318679487No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14690.14405420909924443No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14610.14326970693941193No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14030.13758206628062625No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG12200.1196365793744576No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA12100.11865595167466696No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT12090.1185578889046879No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC11580.11355668763575563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAG2800.032.231613
GTATTAC1606.205846E-826.7340371
CGTATAG2600.025.591561
GTCGGGA1551.4099751E-624.5169262
GTACATG57650.023.2483351
GAGTACT61500.023.17375812-13
ACATATA4000.022.5632342
GTACTTT64900.021.99362814-15
GTATAGG5500.021.6032641
AGTACTT68000.021.55244612-13
ACATGGG58750.021.2634773
GTGGTAC3401.8189894E-1220.9678731
GGACAAT5900.020.9328826
GGTACTC2751.2587407E-920.726933
CCCTATA4150.020.6030792
TACATGG63650.020.2991682
CTTACTA5400.020.2351159
ACTTTTT72550.019.9022116-17
GTATGGT2651.8419087E-819.7282661
CCTATAC4600.019.6192423
Produced by FastQC (version 0.11.5)