##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937064_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1019755 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1447141715412 33.0 31.0 34.0 31.0 34.0 2 32.273840285166536 33.0 31.0 34.0 31.0 34.0 3 32.39032708836927 34.0 31.0 34.0 31.0 34.0 4 36.062660148761225 37.0 35.0 37.0 35.0 37.0 5 35.9152443479071 37.0 35.0 37.0 35.0 37.0 6 36.01562924427926 37.0 35.0 37.0 35.0 37.0 7 35.97114601056136 37.0 35.0 37.0 35.0 37.0 8 35.92360910218631 37.0 35.0 37.0 35.0 37.0 9 37.604053914910935 39.0 37.0 39.0 35.0 39.0 10-11 37.52202734970655 39.0 37.0 39.0 35.0 39.0 12-13 37.43696378051591 39.0 37.0 39.0 34.5 39.0 14-15 38.57643649700174 40.0 38.0 41.0 34.0 41.0 16-17 38.58447274100151 40.0 38.0 41.0 34.5 41.0 18-19 38.75236061603032 40.0 38.0 41.0 35.0 41.0 20-21 38.77153384881663 40.0 38.0 41.0 35.0 41.0 22-23 38.671833920892766 40.0 38.0 41.0 34.0 41.0 24-25 38.5648386131963 40.0 38.0 41.0 34.0 41.0 26-27 38.33825526719653 40.0 38.0 41.0 34.0 41.0 28-29 38.20076194772274 40.0 38.0 41.0 34.0 41.0 30-31 38.13257988438399 40.0 37.0 41.0 34.0 41.0 32-33 37.955054890635495 40.0 37.0 41.0 33.0 41.0 34-35 37.81074915053126 40.0 37.0 41.0 33.0 41.0 36-37 37.62572725801786 40.0 36.5 41.0 33.0 41.0 38-39 37.44253276522302 40.0 36.0 41.0 33.0 41.0 40-41 37.133321238925035 40.0 36.0 41.0 32.0 41.0 42-43 36.90628827512491 39.0 36.0 41.0 31.0 41.0 44-45 36.62388858108075 39.0 35.0 41.0 31.0 41.0 46-47 36.16266456158587 39.0 35.0 41.0 30.0 41.0 48-49 35.73214840819608 39.0 35.0 40.0 28.5 41.0 50-51 35.445653122563755 39.0 35.0 40.0 27.5 41.0 52-53 35.211086976773835 38.5 35.0 40.0 27.0 41.0 54-55 34.83211702810969 38.0 34.0 40.0 26.0 41.0 56-57 34.644439595785265 38.0 34.0 40.0 26.0 41.0 58-59 34.347992409941604 38.0 34.0 40.0 25.0 41.0 60-61 33.962650832798076 37.5 33.5 40.0 24.0 41.0 62-63 33.61292026025859 37.0 33.0 39.5 23.0 40.5 64-65 33.28617216880525 36.0 33.0 39.0 22.0 40.0 66-67 32.956478271741744 36.0 32.5 39.0 22.0 40.0 68-69 32.49920863344627 35.5 32.0 38.5 20.5 40.0 70-71 32.041017695426845 35.0 31.0 38.0 19.5 40.0 72-73 31.632365617231592 35.0 31.0 37.0 18.0 39.0 74-75 31.13893435187864 35.0 31.0 37.0 16.0 39.0 76-77 29.68459875166094 33.5 28.5 35.0 13.0 37.5 78-79 30.358118371569642 34.0 30.0 36.0 11.0 38.0 80-81 30.194534961829067 34.0 30.0 35.5 7.5 37.0 82-83 29.84318586327108 34.0 30.0 35.0 6.0 37.0 84-85 29.404877642178757 34.0 29.0 35.0 2.0 36.0 86-87 29.050604802133847 34.0 29.0 35.0 2.0 36.0 88-89 28.662211511588566 34.0 29.0 35.0 2.0 35.5 90-91 28.189276836102792 33.0 28.5 35.0 2.0 35.0 92-93 27.728827512490746 33.0 27.0 35.0 2.0 35.0 94-95 27.18669484336924 33.0 26.5 34.5 2.0 35.0 96-97 26.42741050546455 32.0 25.0 34.0 2.0 35.0 98-99 25.614815323288436 32.0 24.0 34.0 2.0 35.0 100-101 22.486998837956175 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09403998589486662 1101 2 0.2201612856052506 1101 3 0.18846893737430293 1101 4 0.10448478658681637 1101 5 0.06650383253616354 1101 6 0.1228451700605433 1101 7 0.005624057739524346 1101 8 0.1521634970066046 1101 9 0.10514656841247927 1101 10-11 0.15152982210958754 1101 12-13 0.2084930849966753 1101 14-15 0.30307652046217726 1101 16-17 0.2921796023489023 1101 18-19 0.10026142292982598 1101 20-21 -0.029735794552571804 1101 22-23 0.04666613817579446 1101 24-25 0.19977755929414087 1101 26-27 0.02078582563162712 1101 28-29 0.01792394154747967 1101 30-31 0.07587357655829408 1101 32-33 0.1169018408378335 1101 34-35 0.06033633686185169 1101 36-37 0.030454253909894646 1101 38-39 0.01927373424712897 1101 40-41 0.08239179524945683 1101 42-43 -0.1159303629489159 1101 44-45 0.009457036716653988 1101 46-47 -0.21778853201625736 1101 48-49 0.016164795793464748 1101 50-51 0.03782720086176994 1101 52-53 0.08465314519416012 1101 54-55 -0.06026210189310888 1101 56-57 -0.00124953922446025 1101 58-59 0.04176136573482836 1101 60-61 -0.09015383154103063 1101 62-63 0.026974943379798333 1101 64-65 -0.0381691279424885 1101 66-67 -0.11710796738878315 1101 68-69 -0.2856462773702617 1101 70-71 -0.2914552133949684 1101 72-73 -0.3061856446095277 1101 74-75 -0.28390968232188385 1101 76-77 -0.3705824561087141 1101 78-79 -0.36603895617479765 1101 80-81 -0.2873258592450121 1101 82-83 0.022560500510145687 1101 84-85 -0.32179781152690623 1101 86-87 -0.5479255890135413 1101 88-89 -0.3684535769654609 1101 90-91 -0.34677926544027216 1101 92-93 -0.47722193645795485 1101 94-95 -0.7448570916463098 1101 96-97 -0.7466847110868642 1101 98-99 -0.3246061480318403 1101 100-101 0.11444448936540752 1102 1 -0.1295608714521137 1102 2 -0.01880037193225803 1102 3 0.08068136890562272 1102 4 0.11008532639787916 1102 5 -0.009970724898145988 1102 6 0.07005482315255307 1102 7 0.18024499923352977 1102 8 0.22670108532128808 1102 9 0.0815139574733621 1102 10-11 0.20858110416087072 1102 12-13 0.2905721514410615 1102 14-15 0.2051682883434225 1102 16-17 0.15317031442321394 1102 18-19 0.21518362662911983 1102 20-21 0.21303232484093826 1102 22-23 -0.06588446911165136 1102 24-25 -0.012304682509466147 1102 26-27 0.041993255972180066 1102 28-29 -1.0019118253978831E-4 1102 30-31 0.04195081717114135 1102 32-33 -0.13459653179057085 1102 34-35 0.1183957954631012 1102 36-37 -0.05614374561380231 1102 38-39 0.10552641345646663 1102 40-41 0.177432281079426 1102 42-43 0.12119950924288503 1102 44-45 0.19269114007459365 1102 46-47 0.04622274050894504 1102 48-49 0.26616876499247866 1102 50-51 0.07945417553828804 1102 52-53 0.047902331508360874 1102 54-55 0.2975929429588362 1102 56-57 0.11114230010235815 1102 58-59 -0.02741581930924042 1102 60-61 0.03039471176293773 1102 62-63 -0.07318977837934426 1102 64-65 0.11594296223771039 1102 66-67 0.08147304396312194 1102 68-69 0.03731553893521067 1102 70-71 -0.10741734723635687 1102 72-73 -0.14189454355372177 1102 74-75 -0.13345322443508678 1102 76-77 -0.10306836545220932 1102 78-79 -0.5742214599455338 1102 80-81 -0.18680787877347171 1102 82-83 -0.2383170893922113 1102 84-85 -0.27492157115221616 1102 86-87 -0.30132917716944974 1102 88-89 -0.08544174875239108 1102 90-91 0.07256661056194957 1102 92-93 -0.09115977880136938 1102 94-95 0.008711218268750542 1102 96-97 0.4194738981057995 1102 98-99 0.38114720594132834 1102 100-101 0.3843262072347464 1103 1 0.07099098407999094 1103 2 0.1261503963130508 1103 3 0.18447619690243755 1103 4 0.21758218525589967 1103 5 0.028724038222790682 1103 6 0.004938334004890521 1103 7 0.1343596899234285 1103 8 0.002042504871099027 1103 9 0.15136556417775182 1103 10-11 0.06443060855846738 1103 12-13 0.039346079552025515 1103 14-15 0.11330035591287668 1103 16-17 0.17012878565198974 1103 18-19 0.19873389721899315 1103 20-21 0.14718495559867506 1103 22-23 0.1580091508799697 1103 24-25 0.1039820360031598 1103 26-27 -0.08719965531211216 1103 28-29 -0.21462294290503792 1103 30-31 0.0011155608293194064 1103 32-33 0.14985707374768964 1103 34-35 0.0850943525908292 1103 36-37 0.07024554247613679 1103 38-39 -0.006815194972475069 1103 40-41 0.004578728098827867 1103 42-43 -0.05874645490293062 1103 44-45 -0.09952662934504986 1103 46-47 -0.21173892524070226 1103 48-49 -0.09578317758826671 1103 50-51 0.032458174848464694 1103 52-53 -0.0520377198996087 1103 54-55 0.05486191504578386 1103 56-57 -0.17160646602421536 1103 58-59 -0.2365054219239795 1103 60-61 -0.4368265478144693 1103 62-63 -0.24950721028021405 1103 64-65 -0.12107898880152845 1103 66-67 0.07716003019137219 1103 68-69 0.10921155687051254 1103 70-71 0.1615847140097486 1103 72-73 0.15626142133118748 1103 74-75 -0.11291754681069222 1103 76-77 -0.009708287929644399 1103 78-79 0.0010965550169785843 1103 80-81 -0.0037959136914729186 1103 82-83 0.039302787261505046 1103 84-85 -0.3057663535716735 1103 86-87 -0.494734421439432 1103 88-89 -0.2402926574252291 1103 90-91 -0.5735790234560021 1103 92-93 -0.6650168547882629 1103 94-95 -0.2428909694442538 1103 96-97 -0.4451420613590962 1103 98-99 -0.46352024361562627 1103 100-101 0.18038520321901075 1104 1 -0.0015992512155165173 1104 2 0.09183583806610329 1104 3 0.0287596549002771 1104 4 0.08691572491502342 1104 5 -0.1138686696182063 1104 6 0.07199561800617005 1104 7 0.09290923082050995 1104 8 -0.019180177285704758 1104 9 0.2966477959948719 1104 10-11 0.23555052028691392 1104 12-13 0.16835114070126878 1104 14-15 0.17514048482164668 1104 16-17 0.13089478697404644 1104 18-19 0.14279633992574503 1104 20-21 0.174521721340966 1104 22-23 0.14681247250094742 1104 24-25 0.06719703476688466 1104 26-27 0.19842350184254087 1104 28-29 0.028319369412784567 1104 30-31 0.09322923400590355 1104 32-33 0.04324242584994664 1104 34-35 0.0760254089396355 1104 36-37 0.2593662582023697 1104 38-39 0.29572976078241453 1104 40-41 0.4358196768162159 1104 42-43 0.09916930698588544 1104 44-45 -0.039969820509817566 1104 46-47 -0.05869466667635237 1104 48-49 0.10918790035746895 1104 50-51 0.20116272920998313 1104 52-53 0.270799349444367 1104 54-55 0.2219891704025443 1104 56-57 0.10872720987720186 1104 58-59 -0.09332106926834172 1104 60-61 -0.06686309775568589 1104 62-63 0.02571443576228205 1104 64-65 0.09025468878189713 1104 66-67 -0.09263151908545098 1104 68-69 -0.009034567372374624 1104 70-71 0.034364258825902994 1104 72-73 0.0875662861905937 1104 74-75 -0.13101632752618286 1104 76-77 0.18499247519544681 1104 78-79 0.30499131877114394 1104 80-81 0.2326109731046273 1104 82-83 0.294387484559703 1104 84-85 0.09756384562803078 1104 86-87 -0.2549736191909311 1104 88-89 -0.10298522582811742 1104 90-91 -0.36792294948820725 1104 92-93 -0.4713429549448591 1104 94-95 0.09014282705684451 1104 96-97 0.2543591404325731 1104 98-99 -0.20594323727952002 1104 100-101 0.2818184386036613 1105 1 -0.030880454741605945 1105 2 0.03920657324295007 1105 3 0.056326954510169 1105 4 -9.701297738544667E-4 1105 5 -0.0914439438816288 1105 6 0.07230253440162926 1105 7 -0.006411845973246955 1105 8 0.03810800945051085 1105 9 0.09152763736392444 1105 10-11 0.07040579110184098 1105 12-13 0.10358488426228263 1105 14-15 0.14295004147401613 1105 16-17 -0.0376367991198876 1105 18-19 0.06562454002488494 1105 20-21 -0.07639838125187737 1105 22-23 -0.05024940649984444 1105 24-25 -0.2732538564545308 1105 26-27 -0.15029128575148576 1105 28-29 -0.20571946930523666 1105 30-31 -0.19014641528176668 1105 32-33 -0.06326747780762076 1105 34-35 -0.1555408561206093 1105 36-37 -0.07166936910112298 1105 38-39 -0.14657081899644453 1105 40-41 -0.18568042017281527 1105 42-43 -0.16438609530305826 1105 44-45 -0.1605552031854245 1105 46-47 -0.3419052190827614 1105 48-49 -0.26472463709107075 1105 50-51 -0.20742575711456368 1105 52-53 -0.055204055948230746 1105 54-55 0.028852545189714363 1105 56-57 -0.2203845861440854 1105 58-59 -0.22664132704363738 1105 60-61 -0.09172461897472317 1105 62-63 -0.06529963842354647 1105 64-65 -0.13752857306693045 1105 66-67 -0.2162287344226499 1105 68-69 -0.01962016557915547 1105 70-71 -0.44750784497391294 1105 72-73 -0.2607980477103027 1105 74-75 -0.17084562683432836 1105 76-77 -0.18694924517440015 1105 78-79 -0.25654283214378637 1105 80-81 -0.3902573157933631 1105 82-83 -0.4114603073233951 1105 84-85 -0.7106513406986714 1105 86-87 -0.35512061145336205 1105 88-89 -0.2663319939381239 1105 90-91 -0.16931700513835324 1105 92-93 0.027998381779497095 1105 94-95 -0.039806092054305964 1105 96-97 0.19894564387029234 1105 98-99 0.12371902536677482 1105 100-101 0.25090307769670517 1106 1 -0.056263957576838663 1106 2 0.1100869883121618 1106 3 0.049465456312319134 1106 4 0.11023719821806566 1106 5 -0.07906257608163259 1106 6 0.13659969457518883 1106 7 0.22202315023879038 1106 8 0.16556731751543197 1106 9 0.08014353763712023 1106 10-11 0.11313725876638614 1106 12-13 0.28109400981612964 1106 14-15 0.4059124602623214 1106 16-17 0.35465687153371306 1106 18-19 0.08644411806845653 1106 20-21 0.06549376503247117 1106 22-23 0.16913717262271888 1106 24-25 0.23699201695853844 1106 26-27 0.33284744562435264 1106 28-29 0.3530835335185216 1106 30-31 0.37099272932669436 1106 32-33 0.18887972483707216 1106 34-35 0.15188263844997607 1106 36-37 0.10438655504773209 1106 38-39 0.23792469283807804 1106 40-41 0.3503231611766182 1106 42-43 0.5344467520993206 1106 44-45 0.40288977948176097 1106 46-47 0.2858787061680488 1106 48-49 0.3233797730193544 1106 50-51 0.21514271323599132 1106 52-53 0.17925403537618223 1106 54-55 0.4152163276456875 1106 56-57 0.3563871487442256 1106 58-59 0.2590796491036613 1106 60-61 0.23695602107669345 1106 62-63 0.08187224339192767 1106 64-65 0.13857097008144592 1106 66-67 0.024292856982114586 1106 68-69 -0.026321880494556638 1106 70-71 0.07882976608235737 1106 72-73 -0.17439064625480682 1106 74-75 -0.060900035796883856 1106 76-77 0.01760228263844965 1106 78-79 -0.06910739974176039 1106 80-81 -0.14930045268814496 1106 82-83 0.06909112865998424 1106 84-85 0.06181175533199834 1106 86-87 -0.12255237067648395 1106 88-89 0.04028891104132981 1106 90-91 -0.07199704829820419 1106 92-93 -0.26323144443322377 1106 94-95 -0.06333096636271307 1106 96-97 0.024697842038296614 1106 98-99 -0.14904848536579607 1106 100-101 0.5087570229204239 1107 1 0.1723730589335517 1107 2 0.25029299469994015 1107 3 0.1551616335063244 1107 4 0.06899881646864259 1107 5 0.047171748617167 1107 6 0.027098384699513645 1107 7 0.11556731350505345 1107 8 0.1864989647560975 1107 9 0.2347576882000766 1107 10-11 0.21129737898397138 1107 12-13 0.18061292465517198 1107 14-15 0.3974151117475131 1107 16-17 0.25709188305065567 1107 18-19 0.251782845179811 1107 20-21 0.3203527056296309 1107 22-23 0.17519443220463415 1107 24-25 0.23164803667453526 1107 26-27 0.29064630769732247 1107 28-29 0.36752829367408424 1107 30-31 0.378402339117514 1107 32-33 0.2410975303583669 1107 34-35 0.2695234366588153 1107 36-37 0.32546310392812217 1107 38-39 0.29370889484892615 1107 40-41 0.29792227575338615 1107 42-43 0.2351980143742196 1107 44-45 0.3495476192560929 1107 46-47 0.12263783062625322 1107 48-49 0.011510727388028386 1107 50-51 -0.12421272833807961 1107 52-53 0.03270614118562776 1107 54-55 0.28126530713390707 1107 56-57 0.06271526893837631 1107 58-59 -0.181775778312236 1107 60-61 -0.01675854808014776 1107 62-63 -0.1807961535018734 1107 64-65 0.046002354175271876 1107 66-67 0.18804196233165982 1107 68-69 -0.12421934207950613 1107 70-71 -0.17158301806986032 1107 72-73 0.10501613580590075 1107 74-75 -0.006268993061642192 1107 76-77 -0.11296493307263589 1107 78-79 0.23373849189642115 1107 80-81 0.21933898535908014 1107 82-83 0.22181735927426516 1107 84-85 0.2898165359143974 1107 86-87 0.3333683357092454 1107 88-89 0.38201755635746437 1107 90-91 0.41103217711269835 1107 92-93 0.2848023750626858 1107 94-95 0.6209870439151466 1107 96-97 0.5222309013864539 1107 98-99 0.2273681869153208 1107 100-101 0.5439566971846617 1108 1 0.09099761174758214 1108 2 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2316 84-85 -1.1854702136861484 2316 86-87 -1.3938348332780457 2316 88-89 -2.0218105270329367 2316 90-91 -1.8692320319719862 2316 92-93 -1.7683656611543128 2316 94-95 -2.1221428541294394 2316 96-97 -1.8075905815322102 2316 98-99 -2.3387340157781296 2316 100-101 -2.9356398561136707 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 10.0 5 32.0 6 71.0 7 166.0 8 314.0 9 603.0 10 1053.0 11 1822.0 12 2796.0 13 3854.0 14 4851.0 15 6748.0 16 9330.0 17 13343.0 18 11402.0 19 6404.0 20 5383.0 21 5502.0 22 5870.0 23 6520.0 24 7539.0 25 8673.0 26 10665.0 27 13222.0 28 16382.0 29 20302.0 30 25178.0 31 31691.0 32 40080.0 33 52802.0 34 72456.0 35 104521.0 36 155609.0 37 226426.0 38 141972.0 39 6161.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.19741739944974 21.33367336429577 15.794961329651844 20.67394790660265 2 14.883017594922556 23.043248872736402 39.651533843405 22.422199688936047 3 18.311645011032116 28.161412110811472 31.511841137533708 22.015101740622704 4 11.955616790307475 18.884143740408234 39.64099219910665 29.51924727017764 5 11.840839442006423 39.77650837235529 34.90774473510015 13.474907450538135 6 28.00074527705184 40.0355967855024 17.72023672352673 14.24342121391903 7 25.898014219171365 35.24785486638882 22.565040451090955 16.28909046334886 8 25.722354879358278 34.07612612833475 20.801663144578843 19.39985584772813 9 26.520121519503725 17.605030232781633 22.19741231708824 33.6774359306264 10-11 23.507509156611146 27.832714720692714 28.410647655564325 20.24912846713181 12-13 25.5501579164928 26.322014309328033 28.422369860481357 19.705457913697817 14-15 22.61919938806731 27.177411446279372 28.090112972188443 22.113276193464873 16-17 19.75896171139146 30.489480316350498 28.69954057592265 21.052017396335394 18-19 19.58958507906606 28.95326641814134 32.05305033545036 19.404098167342244 20-21 20.844367540724623 27.522847612468016 31.95738544568228 19.675399401125084 22-23 20.613501324361085 27.391376403915913 31.906903446673013 20.08821882504999 24-25 20.95366511399853 27.895268448149057 31.877469968129446 19.27359646972297 26-27 20.532776335434335 27.53854489202037 32.63355569120939 19.295123081335912 28-29 19.649876514396357 27.893821503116683 32.76102238332854 19.695279599158425 30-31 20.20504925202622 27.684149624174438 32.21195287103275 19.898848252766594 32-33 20.143044302841666 28.20018357368542 31.92549379556246 19.731278327910456 34-35 20.712622149437856 27.57544704365264 31.952723938593092 19.759206868316408 36-37 20.90647124325008 27.166286910955744 31.968566327803956 19.958675517990223 38-39 20.196044298754252 28.17712636717361 31.96036943415378 19.666459899918358 40-41 20.112194495301285 28.10452004271648 32.14875846656082 19.634526995421414 42-43 19.988183436217522 28.513368407117394 31.124142563654996 20.374305593010085 44-45 20.40516031221546 27.985931770163997 30.97628866838703 20.632619249233507 46-47 21.144787583261625 28.56766715453014 30.333536814079885 19.954008448128345 48-49 20.90003035044219 28.789870503145607 30.06306910143569 20.247030044976512 50-51 20.568059905818576 28.818691933386546 30.21322227169046 20.400025889104416 52-53 20.870066192694285 29.19519490071096 29.655552831576365 20.279186075018387 54-55 20.666532647547694 28.86507053164731 29.614024937362405 20.85437188344259 56-57 21.025780325677704 29.24396702918998 29.169193837119963 20.56105880801235 58-59 21.332988152044514 28.461668206253666 29.449602551203526 20.755741090498297 60-61 20.86030742496655 29.255385134376453 29.839118941953668 20.045188498703332 62-63 20.754249795293966 28.990002500600635 30.151801167927587 20.10394653617781 64-65 20.672299727336068 29.205342271441086 29.669474044462707 20.452883956760136 66-67 20.729292820334297 29.13204642291531 29.05227235953734 21.086388397213057 68-69 20.485469787090913 29.02639802030783 29.63100432506059 20.85712786754067 70-71 21.098610250335376 29.166895480469762 29.264468223646535 20.47002604554833 72-73 21.000046579929936 28.705445684124335 29.55874096900964 20.735766766936095 74-75 20.578999807791128 30.182578815444177 28.634807065441237 20.603614311323458 76-77 20.836358982832614 29.163420375610382 29.208921567810258 20.791299073746742 78-79 20.775423068135126 29.25566289091574 29.014869286974392 20.95404475397474 80-81 20.970440301529912 29.94200442664894 28.507266117699437 20.58028915412172 82-83 20.99296545684815 29.35807526034478 28.9910257724048 20.65793351040227 84-85 20.552137161999493 29.399835348330463 28.625364496530203 21.42266299313984 86-87 20.539503722473697 29.56454118256204 28.780281872455266 21.115673222509002 88-89 20.89599515164115 28.976200971535015 28.673080477951686 21.45472339887215 90-91 20.716359071920294 28.70015886402416 29.146988448035778 21.43649361601977 92-93 20.949492770322284 29.21368367892288 28.942589151315758 20.894234399439082 94-95 20.58533667400503 28.49704095591588 29.06379473500988 21.85382763506921 96-97 20.450001127724065 28.79182507921036 29.005945557404587 21.75222823566099 98-99 20.523912980589838 29.215802508361122 28.332007027216 21.92827748383304 100-101 20.023084530667663 29.16883431455664 28.703711421792327 22.104369732983372 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2320.0 1 1546.5 2 829.0 3 883.5 4 753.5 5 811.0 6 1363.0 7 2535.0 8 3738.5 9 4085.5 10 3484.0 11 2784.0 12 2651.5 13 2636.5 14 2552.5 15 2500.0 16 2441.5 17 2416.0 18 2467.5 19 2529.5 20 2713.5 21 2989.0 22 3350.0 23 3845.5 24 4201.5 25 5006.5 26 6804.0 27 8452.0 28 9060.5 29 10656.5 30 13678.0 31 16268.5 32 20158.0 33 25290.0 34 30289.5 35 34083.5 36 39019.5 37 43823.0 38 43685.5 39 41058.0 40 37991.5 41 35553.5 42 32968.0 43 32307.5 44 33458.0 45 33505.5 46 32003.5 47 33953.0 48 45143.5 49 43159.5 50 29958.5 51 24617.5 52 22258.5 53 21473.5 54 20571.0 55 19053.0 56 17053.5 57 15162.0 58 14380.0 59 13646.5 60 11967.5 61 9927.0 62 8061.5 63 6326.5 64 4567.0 65 3361.0 66 2514.5 67 1980.5 68 1440.5 69 827.5 70 585.5 71 413.0 72 272.0 73 220.5 74 155.5 75 124.0 76 86.0 77 40.0 78 24.0 79 15.0 80 10.0 81 7.0 82 4.5 83 3.5 84 1.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023535064794975264 2 0.002843820329392844 3 4.90313849895318E-4 4 0.0 5 0.002941883099371908 6 0.0 7 4.90313849895318E-4 8 0.0 9 9.80627699790636E-5 10-11 0.0 12-13 0.006913425283523984 14-15 0.003432196949267226 16-17 0.0 18-19 6.374080048639134E-4 20-21 9.31596314801104E-4 22-23 0.0017651298596231448 24-25 4.90313849895318E-4 26-27 2.45156924947659E-4 28-29 4.90313849895318E-5 30-31 0.0 32-33 9.80627699790636E-5 34-35 0.0 36-37 0.0020102867845708037 38-39 0.008580492373168064 40-41 7.845021598325088E-4 42-43 0.0 44-45 0.0010296590847801677 46-47 2.45156924947659E-4 48-49 4.90313849895318E-5 50-51 0.0020593181695603355 52-53 4.90313849895318E-4 54-55 0.0 56-57 4.412824649057862E-4 58-59 9.80627699790636E-5 60-61 0.0025986634044451854 62-63 0.0 64-65 1.470941549685954E-4 66-67 0.0 68-69 4.90313849895318E-5 70-71 2.941883099371908E-4 72-73 2.45156924947659E-4 74-75 0.0030399458693509715 76-77 1.470941549685954E-4 78-79 1.961255399581272E-4 80-81 0.0021573809395393994 82-83 1.470941549685954E-4 84-85 0.0025986634044451854 86-87 2.941883099371908E-4 88-89 0.002794788944403313 90-91 0.001470941549685954 92-93 0.0 94-95 0.0 96-97 1.961255399581272E-4 98-99 5.393452348848498E-4 100-101 0.002402537864487058 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1019755.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.99322394796313 #Duplication Level Percentage of deduplicated Percentage of total 1 70.04113821199839 24.509652350226975 2 14.426415877357194 10.096536031256225 3 5.536512216031121 5.812212355986319 4 2.7879482485330476 3.90237189664994 5 1.5667136310602945 2.7412180477009684 6 1.0273973957119173 2.1571168291020735 7 0.6927257732448453 1.696849568237796 8 0.4871932481653387 1.363876995118822 9 0.3758729625365464 1.1837706078623151 >10 2.514558742778763 17.473350255198675 >50 0.32035178704259065 7.865183153015192 >100 0.2053746523141415 13.221194503101247 >500 0.012147331566830018 2.6977389488860926 >1k 0.0048024334101421 3.1381356882517046 >5k 8.474882488486059E-4 2.1407927694055413 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8884 0.871189648494001 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6850 0.6717299743565857 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5922 0.5807277238160147 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4966 0.4869797157160298 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2875 0.28193046368980784 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2467 0.24192085353834988 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2437 0.23897897043897798 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2259 0.2215237973827047 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1765 0.17308078901304727 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1629 0.1597442522958946 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1623 0.15915587567602021 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1586 0.15552755318679487 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1469 0.14405420909924443 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1461 0.14326970693941193 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1403 0.13758206628062625 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1220 0.1196365793744576 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1210 0.11865595167466696 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1209 0.1185578889046879 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1158 0.11355668763575563 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.470941549685954E-4 0.0 18-19 0.0 0.0 0.0 1.9612553995812718E-4 0.0 20-21 0.0 0.0 0.0 1.9612553995812718E-4 0.0 22-23 0.0 0.0 0.0 2.45156924947659E-4 0.0 24-25 0.0 0.0 0.0 5.883766198743816E-4 0.0 26-27 0.0 0.0 0.0 0.001421910164696422 0.0 28-29 0.0 0.0 0.0 0.0033831655642776945 0.0 30-31 0.0 0.0 0.0 0.006668268358576325 0.0 32-33 0.0 0.0 0.0 0.010443685002770275 0.0 34-35 0.0 0.0 0.0 0.014856509651828134 0.0 36-37 0.0 0.0 0.0 0.02044608754063476 0.0 38-39 0.0 0.0 0.0 0.025006006344661217 0.0 40-41 0.0 0.0 0.0 0.031183960853342227 0.0 42-43 0.0 0.0 0.0 0.04084314369627999 0.0 44-45 0.0 0.0 0.0 0.05579771611808719 0.0 46-47 0.0 0.0 0.0 0.09070806223063382 0.0 48-49 0.0 0.0 0.0 0.16577511264960704 0.0 50-51 0.0 0.0 0.0 0.21470843486915975 0.0 52-53 0.0 0.0 0.0 0.2549141705605758 0.0 54-55 0.0 0.0 0.0 0.3058577795646994 0.0 56-57 0.0 0.0 0.0 0.3452299817112934 0.0 58-59 0.0 0.0 0.0 0.39749743811013427 0.0 60-61 0.0 0.0 0.0 0.4905099754352761 0.0 62-63 0.0 0.0 0.0 0.5560649371662801 0.0 64-65 0.0 0.0 0.0 0.6289255752607243 0.0 66-67 0.0 0.0 0.0 0.7612122519624811 0.0 68-69 0.0 0.0 0.0 0.9066393398414325 0.0 70-71 0.0 0.0 0.0 1.0281391118454923 0.0 72-73 0.0 0.0 0.0 1.1705752852400821 0.0 74-75 0.0 0.0 0.0 1.295115003113493 0.0 76-77 0.0 0.0 0.0 1.385087594569284 0.0 78-79 0.0 0.0 0.0 1.5028609813141391 0.0 80-81 0.0 0.0 0.0 1.675353393707312 0.0 82-83 0.0 0.0 0.0 1.8659874185466117 0.0 84-85 0.0 0.0 0.0 2.1057018597604324 0.0 86-87 0.0 0.0 0.0 2.5175654936724996 0.0 88-89 0.0 0.0 0.0 3.028717682188369 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATAG 280 0.0 32.23161 3 GTATTAC 160 6.205846E-8 26.734037 1 CGTATAG 260 0.0 25.59156 1 GTCGGGA 155 1.4099751E-6 24.516926 2 GTACATG 5765 0.0 23.248335 1 GAGTACT 6150 0.0 23.173758 12-13 ACATATA 400 0.0 22.563234 2 GTACTTT 6490 0.0 21.993628 14-15 GTATAGG 550 0.0 21.603264 1 AGTACTT 6800 0.0 21.552446 12-13 ACATGGG 5875 0.0 21.263477 3 GTGGTAC 340 1.8189894E-12 20.967873 1 GGACAAT 590 0.0 20.932882 6 GGTACTC 275 1.2587407E-9 20.72693 3 CCCTATA 415 0.0 20.603079 2 TACATGG 6365 0.0 20.299168 2 CTTACTA 540 0.0 20.235115 9 ACTTTTT 7255 0.0 19.90221 16-17 GTATGGT 265 1.8419087E-8 19.728266 1 CCTATAC 460 0.0 19.619242 3 >>END_MODULE