FastQCFastQC Report
Thu 26 May 2016
SRR937063_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937063_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407645
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85030.6040585516944968No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58790.41764791549005603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41380.29396616334374076No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14780.10499806414259276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32200.049.4429631
GTATCAA62700.047.1452981
TACGGTA651.3254573E-436.5519374
TATCAAC81700.036.1231042
ATCAACG81850.035.7034533
TCAACGC82200.035.493624
CAACGCA83250.035.1030275
AACGCAG85200.034.2438476
ACGCAGA98450.029.3455057
CGCAGAG98500.029.1376468
CTACGGT700.00837711827.1528663
GCAGAGT104250.027.120319
GTACATG57700.025.8624321
TACACCG1551.4064881E-624.525175
TACATGG59750.024.1797162
ACATGGG59150.023.6181743
AGTACTT66600.023.25477612-13
AGAGTAC93550.023.15946210-11
GTGGTAT16100.023.0241851
TGGTATC16300.022.4501192
Produced by FastQC (version 0.11.5)