##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937063_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1407645 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.246217618788826 33.0 31.0 34.0 31.0 34.0 2 32.376862063943676 34.0 31.0 34.0 31.0 34.0 3 32.39800731008173 34.0 31.0 34.0 31.0 34.0 4 35.948745599920436 37.0 35.0 37.0 35.0 37.0 5 35.971978730432745 37.0 35.0 37.0 35.0 37.0 6 36.04355856767864 37.0 37.0 37.0 35.0 37.0 7 36.00365930330445 37.0 37.0 37.0 35.0 37.0 8 35.98481861548899 37.0 36.0 37.0 35.0 37.0 9 37.691784505326275 39.0 38.0 39.0 35.0 39.0 10-11 37.583463160100735 39.0 37.5 39.0 35.0 39.0 12-13 37.50661494908162 39.0 37.0 39.0 35.0 39.0 14-15 38.78269378998256 40.0 38.0 41.0 35.5 41.0 16-17 38.7140830962352 40.0 38.0 41.0 35.0 41.0 18-19 38.67016612853383 40.0 38.0 41.0 35.0 41.0 20-21 38.620120484923405 40.0 38.0 41.0 35.0 41.0 22-23 38.488026455533884 40.0 38.0 41.0 34.5 41.0 24-25 38.34128704325309 40.0 38.0 41.0 34.0 41.0 26-27 38.30173907483776 40.0 38.0 41.0 34.0 41.0 28-29 38.372341748097 40.0 38.0 41.0 34.0 41.0 30-31 38.23554056598077 40.0 38.0 41.0 34.0 41.0 32-33 38.04698876492297 40.0 38.0 41.0 34.0 41.0 34-35 37.86304181807203 40.0 38.0 41.0 33.5 41.0 36-37 37.71678832376061 40.0 37.0 41.0 33.0 41.0 38-39 37.555199286751986 40.0 37.0 41.0 33.0 41.0 40-41 37.34035285885291 40.0 37.0 41.0 33.0 41.0 42-43 37.13390414486607 40.0 37.0 41.0 32.0 41.0 44-45 36.86985852256784 39.5 36.0 41.0 31.5 41.0 46-47 36.49609596169489 39.0 36.0 41.0 30.5 41.0 48-49 36.13319729051004 39.0 36.0 40.5 30.0 41.0 50-51 35.50949173974972 38.5 35.0 39.5 29.0 40.5 52-53 35.82666545897581 39.0 35.5 40.0 30.0 40.5 54-55 36.12433355000728 39.0 36.0 41.0 30.0 41.0 56-57 36.00300999186585 39.0 35.5 41.0 30.0 41.0 58-59 35.76910549179658 39.0 35.0 41.0 29.0 41.0 60-61 35.49028661345723 38.5 35.0 40.0 28.5 41.0 62-63 35.1881262676314 38.0 35.0 40.0 28.0 41.0 64-65 34.87509244163124 37.5 35.0 40.0 28.0 41.0 66-67 34.503781848406376 37.0 34.0 40.0 27.5 41.0 68-69 34.08176564403667 36.5 34.0 39.0 26.0 41.0 70-71 33.719995808602306 36.0 34.0 39.0 26.0 41.0 72-73 33.34286805266953 35.5 34.0 38.5 26.0 40.0 74-75 32.91481836684676 35.0 33.0 37.5 25.5 40.0 76-77 32.48646356148035 35.0 33.0 37.0 24.5 39.0 78-79 32.072613833743596 35.0 32.5 36.5 23.5 39.0 80-81 31.660228253572456 35.0 32.0 36.0 21.5 38.5 82-83 31.261890249317123 35.0 32.0 36.0 20.0 37.0 84-85 30.92826742538069 35.0 31.5 35.0 19.5 37.0 86-87 30.60668883134597 34.0 31.0 35.0 18.0 36.0 88-89 30.265374792650135 34.0 31.0 35.0 14.0 36.0 90-91 29.952401351192947 34.0 31.0 35.0 7.5 36.0 92-93 29.6190335631498 34.0 30.0 35.0 3.5 35.0 94-95 29.259362978591902 34.0 30.0 35.0 2.0 35.0 96-97 28.86164515911327 34.0 29.5 35.0 2.0 35.0 98-99 28.305518792024976 34.0 29.0 35.0 2.0 35.0 100-101 26.093138895104946 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32789901227098284 1101 2 0.29888002404087644 1101 3 0.3904254057253951 1101 4 0.27101153477299533 1101 5 0.23150137083096922 1101 6 0.24863915689292782 1101 7 0.28691012846266517 1101 8 0.18428804226100937 1101 9 0.36247601551862374 1101 10-11 0.35527756381602416 1101 12-13 0.4738282990444205 1101 14-15 0.5963910487542492 1101 16-17 0.5658835483850524 1101 18-19 0.6339173913249354 1101 20-21 0.5931622179280325 1101 22-23 0.6325846100188315 1101 24-25 0.6863145589527733 1101 26-27 0.6641346677389492 1101 28-29 0.568934285282424 1101 30-31 0.5199432102426087 1101 32-33 0.48577621167088836 1101 34-35 0.560559705243115 1101 36-37 0.5100151283565069 1101 38-39 0.5640330901922397 1101 40-41 0.6254351553578843 1101 42-43 0.6036050445695622 1101 44-45 0.5937259310277128 1101 46-47 0.5315066350849236 1101 48-49 0.6416622949076611 1101 50-51 0.5279260493740239 1101 52-53 0.6916685436014731 1101 54-55 0.7050305384100781 1101 56-57 0.6128909683749768 1101 58-59 0.5704247025753659 1101 60-61 0.6900895249025538 1101 62-63 0.7504907193392185 1101 64-65 0.7466581569315878 1101 66-67 0.6544962451462766 1101 68-69 0.6145497587341779 1101 70-71 0.6650631259771984 1101 72-73 0.7414464182470155 1101 74-75 0.7601520300001141 1101 76-77 0.765301247397332 1101 78-79 0.8154668207509133 1101 80-81 0.9352862071924086 1101 82-83 0.8095649376717766 1101 84-85 0.978162641201525 1101 86-87 1.077928050305733 1101 88-89 1.0512965817687423 1101 90-91 1.1214204566794024 1101 92-93 0.9544434304770562 1101 94-95 1.1385181743284605 1101 96-97 1.0838869293776376 1101 98-99 1.1810492059385886 1101 100-101 1.2527707112474644 1102 1 0.334673005416775 1102 2 0.3090304889528781 1102 3 0.33451194353050084 1102 4 0.2154001629753779 1102 5 0.17131199257966045 1102 6 0.14915982192417232 1102 7 0.23783323934519984 1102 8 0.15996777410031626 1102 9 0.19618463880564008 1102 10-11 0.29574356812153013 1102 12-13 0.32206912027517376 1102 14-15 0.42728499738863945 1102 16-17 0.5331232386257696 1102 18-19 0.5118512523019163 1102 20-21 0.45303750181336255 1102 22-23 0.4584047484683964 1102 24-25 0.5925888797898438 1102 26-27 0.46894501641157404 1102 28-29 0.5163386972813839 1102 30-31 0.5118024144989519 1102 32-33 0.5741905613629896 1102 34-35 0.31311040636591514 1102 36-37 0.3557487699623749 1102 38-39 0.3798594980131398 1102 40-41 0.40204044438614517 1102 42-43 0.3844114381905044 1102 44-45 0.3739560203690999 1102 46-47 0.24909279058353917 1102 48-49 0.3479058377965316 1102 50-51 0.11806874299632852 1102 52-53 0.2855799713796756 1102 54-55 0.43072477741535664 1102 56-57 0.4141464924762204 1102 58-59 0.2518413643115025 1102 60-61 0.39015130836232714 1102 62-63 0.46330913740957413 1102 64-65 0.5404060804156643 1102 66-67 0.2754524437454009 1102 68-69 0.40344489297382324 1102 70-71 0.4479625338572646 1102 72-73 0.42659967081051775 1102 74-75 0.38847815336677627 1102 76-77 0.3471270681046832 1102 78-79 0.38113450310883223 1102 80-81 0.27395770351271054 1102 82-83 0.439619689422873 1102 84-85 0.5547991633047538 1102 86-87 0.5705828730566083 1102 88-89 0.45151003622648034 1102 90-91 0.4035435889528962 1102 92-93 0.4021735425747792 1102 94-95 0.4540168908709532 1102 96-97 0.2602380301017462 1102 98-99 0.3542548117350819 1102 100-101 0.7836103860138692 1103 1 0.19116612558435264 1103 2 0.24855897669600324 1103 3 0.18400469913287054 1103 4 0.22832797787600612 1103 5 0.13490000301548832 1103 6 0.0940582246060444 1103 7 0.08405975190488846 1103 8 0.018648879291035314 1103 9 0.1481522815649825 1103 10-11 0.14818520503120425 1103 12-13 -0.1022179884852008 1103 14-15 0.1053967752199867 1103 16-17 0.21648172735452675 1103 18-19 0.2794916960027152 1103 20-21 0.011758032297301213 1103 22-23 -0.051034165483521576 1103 24-25 0.2672928041502729 1103 26-27 0.19190009651498485 1103 28-29 0.12984316647087724 1103 30-31 0.3134272828339064 1103 32-33 0.20573299283482527 1103 34-35 0.2866304437961915 1103 36-37 0.3340458585469648 1103 38-39 0.19577514451075473 1103 40-41 0.25101158673400903 1103 42-43 0.48325314207567516 1103 44-45 0.4924308077753352 1103 46-47 0.37300963409006016 1103 48-49 0.43968542665722055 1103 50-51 0.3652285187471591 1103 52-53 0.35699170562945426 1103 54-55 0.3273346178260468 1103 56-57 0.36454276417349263 1103 58-59 0.2936802692383509 1103 60-61 0.4612213369456768 1103 62-63 0.48791075788412996 1103 64-65 0.497786388431706 1103 66-67 0.36510846993709123 1103 68-69 0.43810708827390243 1103 70-71 0.3569147473143488 1103 72-73 0.34422752691769887 1103 74-75 0.3349051767927733 1103 76-77 0.28687732312221215 1103 78-79 0.39977809489555227 1103 80-81 0.4167764219769303 1103 82-83 0.4201448414536948 1103 84-85 0.37748450077695495 1103 86-87 0.4620656440847206 1103 88-89 0.5413047040743244 1103 90-91 0.45116774003252047 1103 92-93 0.4180573638357039 1103 94-95 0.4157912784429598 1103 96-97 0.06429877592854183 1103 98-99 0.265775606795998 1103 100-101 0.5779065435915456 1104 1 0.31598766616535556 1104 2 0.33915479064412324 1104 3 0.2668554063645061 1104 4 0.23253917120992895 1104 5 0.24173321897928446 1104 6 0.1616592297510664 1104 7 0.14476609845416277 1104 8 0.14785221592097741 1104 9 0.268750442903837 1104 10-11 0.27715540599606925 1104 12-13 0.31485979224285643 1104 14-15 0.42983407487403724 1104 16-17 0.4288102748327489 1104 18-19 0.4182705910358706 1104 20-21 0.29938619277673695 1104 22-23 0.4051395363003607 1104 24-25 0.3729609831522893 1104 26-27 0.32036362024351206 1104 28-29 0.47831268053533904 1104 30-31 0.3201545455325885 1104 32-33 0.4588461717021559 1104 34-35 0.3545133282391433 1104 36-37 0.4533054940728647 1104 38-39 0.4765464868462672 1104 40-41 0.39280072177824366 1104 42-43 0.567655307832247 1104 44-45 0.40301349942439657 1104 46-47 0.37506167689723924 1104 48-49 0.3599251298854611 1104 50-51 0.4329016772960941 1104 52-53 0.47765207923657726 1104 54-55 0.48747348587061623 1104 56-57 0.47541272257465295 1104 58-59 0.472124295513737 1104 60-61 0.4751475852636915 1104 62-63 0.5282082736263973 1104 64-65 0.5317770553087442 1104 66-67 0.49989455593881615 1104 68-69 0.3171157710541834 1104 70-71 0.31843453530520094 1104 72-73 0.4771934230501458 1104 74-75 0.4294553179568936 1104 76-77 0.5477865971114539 1104 78-79 0.564419976707633 1104 80-81 0.49910313515528415 1104 82-83 0.6179731202005705 1104 84-85 0.6367249467042306 1104 86-87 0.6134027588977808 1104 88-89 0.5783270966427132 1104 90-91 0.5551927455161803 1104 92-93 0.437406020627936 1104 94-95 0.42940101382917106 1104 96-97 0.4117119641597782 1104 98-99 0.592494557422544 1104 100-101 0.736049167461303 1105 1 0.3430596528086767 1105 2 0.31527811295495667 1105 3 0.28408076243444214 1105 4 0.28849523790685083 1105 5 0.2992244658184191 1105 6 0.2804205764410739 1105 7 0.3213739514442864 1105 8 0.3811638014317964 1105 9 0.3882878221426367 1105 10-11 0.3839071593298371 1105 12-13 0.343751977075037 1105 14-15 0.4203595887170053 1105 16-17 0.5216896865338825 1105 18-19 0.4386561353527938 1105 20-21 0.4669892990682101 1105 22-23 0.5047313931591688 1105 24-25 0.4502187718445043 1105 26-27 0.4459059650099846 1105 28-29 0.47062293416087186 1105 30-31 0.48688132264036454 1105 32-33 0.469724810747735 1105 34-35 0.4171156510686913 1105 36-37 0.46362220180375147 1105 38-39 0.4908135987354356 1105 40-41 0.5896870684682511 1105 42-43 0.5340042671786804 1105 44-45 0.5325835814447615 1105 46-47 0.5688742863117469 1105 48-49 0.6606681354615489 1105 50-51 0.4765074122374102 1105 52-53 0.45797300145230935 1105 54-55 0.5831456691899035 1105 56-57 0.6245973818497603 1105 58-59 0.5991150317518219 1105 60-61 0.43724389975071887 1105 62-63 0.3903628749793526 1105 64-65 0.4961102886831412 1105 66-67 0.5836415706787079 1105 68-69 0.675392235857835 1105 70-71 0.6205972279287693 1105 72-73 0.652589273943839 1105 74-75 0.629312741393754 1105 76-77 0.5076804380200528 1105 78-79 0.6248934443641616 1105 80-81 0.686776712496485 1105 82-83 0.7222046250958591 1105 84-85 0.7273819605796099 1105 86-87 0.8775449221172131 1105 88-89 0.8009258533696801 1105 90-91 0.6582946468972359 1105 92-93 0.7258469854207767 1105 94-95 0.7842852910990494 1105 96-97 0.942283864352131 1105 98-99 0.8552599213811334 1105 100-101 0.8698512946955184 1106 1 0.3967182249027843 1106 2 0.275224537366789 1106 3 0.2814602173610652 1106 4 0.19389210489963205 1106 5 0.22595111385010114 1106 6 0.1679657080506658 1106 7 0.2262030336711618 1106 8 0.1292318773693708 1106 9 0.1766351317163668 1106 10-11 0.25459272303819347 1106 12-13 0.2921825617477509 1106 14-15 0.41392469470348914 1106 16-17 0.49679742005357497 1106 18-19 0.4748793475587192 1106 20-21 0.4967579631725627 1106 22-23 0.5076627639954125 1106 24-25 0.6007101240457615 1106 26-27 0.6864734586042118 1106 28-29 0.6166797417546377 1106 30-31 0.570090908011899 1106 32-33 0.6831143402143098 1106 34-35 0.6554869823055824 1106 36-37 0.6508936320390148 1106 38-39 0.6103221009483448 1106 40-41 0.6423170766210688 1106 42-43 0.6457035450729265 1106 44-45 0.8342308851950477 1106 46-47 0.7734071013595027 1106 48-49 0.9189075659297501 1106 50-51 0.9046593456173326 1106 52-53 0.8185614594071211 1106 54-55 0.8653594474335478 1106 56-57 0.9695135244074109 1106 58-59 0.8937960531820579 1106 60-61 1.0059640546540507 1106 62-63 0.9863316031414797 1106 64-65 0.8593098286318934 1106 66-67 0.9954303838629599 1106 68-69 0.9524027762724572 1106 70-71 1.021606865711206 1106 72-73 0.9685069014770278 1106 74-75 0.972760650781126 1106 76-77 1.0324467727510154 1106 78-79 1.0180982254030653 1106 80-81 1.0327832929763368 1106 82-83 1.1026873877327112 1106 84-85 1.248367431113035 1106 86-87 1.3048116936056822 1106 88-89 1.2797525771059952 1106 90-91 1.291803411164647 1106 92-93 1.3154638034967157 1106 94-95 1.282837439946011 1106 96-97 1.1487871837092243 1106 98-99 1.300789708243471 1106 100-101 1.64890076476307 1107 1 0.23622204916670597 1107 2 0.3304340694522452 1107 3 0.3800475491443436 1107 4 0.30693805086673365 1107 5 0.2840587882122634 1107 6 0.26657809130689003 1107 7 0.27645981126888586 1107 8 0.3139105530858757 1107 9 0.2725333444503377 1107 10-11 0.3461169094524692 1107 12-13 0.41881912750395855 1107 14-15 0.4184946313702369 1107 16-17 0.5652080998096309 1107 18-19 0.5093484129039112 1107 20-21 0.5812577557150505 1107 22-23 0.640566503710609 1107 24-25 0.6561370950878214 1107 26-27 0.6175601836721256 1107 28-29 0.5355724666503221 1107 30-31 0.6107936801139573 1107 32-33 0.6520557980534605 1107 34-35 0.6092670147159325 1107 36-37 0.583229603605588 1107 38-39 0.7369116951927666 1107 40-41 0.8731159054647009 1107 42-43 0.8007156010942893 1107 44-45 0.7618961270591882 1107 46-47 1.0018193859755442 1107 48-49 0.8591662615308735 1107 50-51 0.8737317365455652 1107 52-53 0.9322558079513925 1107 54-55 0.9248602185492061 1107 56-57 0.8195205701341806 1107 58-59 0.8860823523749275 1107 60-61 0.9044106676166237 1107 62-63 0.8180015578139646 1107 64-65 0.7497865503335106 1107 66-67 0.8504259998551973 1107 68-69 0.9125771971528636 1107 70-71 1.0044342767197634 1107 72-73 0.9186348988466264 1107 74-75 1.0666136456064308 1107 76-77 1.0119478178671457 1107 78-79 1.1390318624855027 1107 80-81 1.109645321989067 1107 82-83 1.0358203092824532 1107 84-85 1.186052479297981 1107 86-87 1.171887012424822 1107 88-89 1.1199586254709963 1107 90-91 1.1622438473734213 1107 92-93 1.0920680763385668 1107 94-95 1.0420814334639417 1107 96-97 1.2212254748856246 1107 98-99 1.2091066015476777 1107 100-101 1.5347226096474813 1108 1 0.1532808624113997 1108 2 0.1882178041148208 1108 3 0.06893313075866558 1108 4 0.12813413666464157 1108 5 0.16376193520721216 1108 6 0.06926628008650226 1108 7 0.09407977613880547 1108 8 0.0956179226839069 1108 9 0.061682081827200363 1108 10-11 0.10273983338549186 1108 12-13 0.026928342049465925 1108 14-15 0.27683742810490486 1108 16-17 0.3005934880336767 1108 18-19 0.3174385229636627 1108 20-21 0.25061027995919716 1108 22-23 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2265.0 12 3133.0 13 4016.0 14 4828.0 15 6660.0 16 9069.0 17 13199.0 18 11168.0 19 6364.0 20 5461.0 21 5575.0 22 5807.0 23 6150.0 24 6804.0 25 7865.0 26 9436.0 27 11297.0 28 13425.0 29 17019.0 30 21545.0 31 28401.0 32 38900.0 33 55656.0 34 84198.0 35 134459.0 36 220476.0 37 332690.0 38 285058.0 39 45171.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.887635386117864 18.70037856225949 14.794207414588934 23.61777863703371 2 14.840258294239856 19.893306447828515 41.07125843786306 24.19517682006857 3 18.481977454223887 24.750379957035353 30.22993620702647 26.537706381714294 4 12.348700484926592 16.787009258982664 37.7648141588892 33.09947609720154 5 13.560075319924794 36.86879030170636 34.36969340125779 15.20144097711105 6 30.823097910914317 36.36362989926111 17.988365288140802 14.824906901683768 7 27.45655203800097 33.01689563955969 21.30294109281224 18.223611229627103 8 25.807575506558937 34.22139204963591 20.221188693694046 19.749843750111097 9 26.01807219490548 16.548162413646455 20.731962296730476 36.70180309471759 10-11 24.058785903415135 26.59090044447826 27.80817568357955 21.542137968527054 12-13 25.45134535309364 24.118736755589005 28.348629691156603 22.081288200160753 14-15 22.4868636173124 25.06102938902356 27.011552480297645 25.440554513366386 16-17 21.43119225608451 28.530302911790155 27.384615275218888 22.653889556906446 18-19 21.27352353597759 26.867024037196867 30.320673695585715 21.538778731239823 20-21 22.047574331783444 25.859826111928985 30.489484043910615 21.60311551237696 22-23 21.76040553710848 25.852030991087823 30.410058241577794 21.97750523022591 24-25 21.5210099503796 26.06826732324909 30.40345985197042 22.00726287440089 26-27 21.36688290227179 26.30819934994275 30.235338351743092 22.089579396042367 28-29 21.20381631801513 26.297713749137223 30.455942240586975 22.04252769226067 30-31 21.346864832364158 26.11851989782792 30.39029507376489 22.144320196043036 32-33 21.18696861201681 26.279871000986148 30.27500244316314 22.2581579438339 34-35 21.639793400103407 26.37233110707198 29.973970687262693 22.013904805561925 36-37 21.580669383299284 26.17647403734795 30.06485732348314 22.177999255869622 38-39 21.409361725112596 26.296581373349625 30.13840530423368 22.1556515973041 40-41 21.637370577199125 26.230256281401676 30.132282870183953 22.000090271215246 42-43 21.421661655685153 26.32531576291305 29.903760723855825 22.34926185754597 44-45 21.552412469986486 26.565402187304226 29.579919186937154 22.302266155772134 46-47 21.721472967676902 26.61014124722368 29.27832201723477 22.39006376786465 48-49 21.8557584613023 26.828212679587693 28.89364883534502 22.422380023764987 50-51 21.77303515693697 27.02026706553 28.769853606024164 22.436844171508866 52-53 21.940235769494937 27.08595456372126 28.55971821647487 22.414091450308934 54-55 21.766692891169733 26.977373726174967 28.668356690197648 22.58757669245765 56-57 21.710949004713466 27.3281631121396 28.52276991568067 22.43811796746627 58-59 21.93335989148354 27.018842900960248 28.554797151762774 22.493000055793438 60-61 21.892111929990275 26.91491036453686 28.93622586139561 22.256751844077257 62-63 21.70107558827564 26.914547841396043 29.05396136752453 22.330415202803795 64-65 21.648537238909412 27.01377713329171 28.826696220482457 22.510989407316416 66-67 21.60648467839067 27.303359086435965 28.488494621630544 22.601661613542824 68-69 21.53021597664257 27.186880776458345 28.74327294023329 22.539630306665796 70-71 21.65665900563368 27.053264015728978 28.59308545882885 22.696991519808492 72-73 21.74268307370056 27.111182321784533 28.52804577504193 22.618088829472978 74-75 21.597757383898784 27.349932671775797 28.355912270790924 22.696397673534502 76-77 21.683795935525296 27.19728490161819 28.384909471254417 22.734009691602093 78-79 21.738408824926957 27.20337968061919 28.361226644660505 22.696984849793356 80-81 21.63456516038435 27.393541986609826 28.268643521555255 22.70324933145057 82-83 21.619848480163707 27.393694622527814 28.3455536494445 22.640903247863978 84-85 21.63116194085788 27.296019459331127 28.17682856831113 22.895990031499863 86-87 21.68088611351508 27.160879076354966 28.40148064692115 22.756754163208804 88-89 21.745060921961233 27.19723429404289 28.125700980261463 22.932003803734414 90-91 21.61866688654916 27.30432353434574 28.25857328484545 22.818436294259644 92-93 21.732701596994396 27.268545999338876 28.197667599105714 22.801084804561015 94-95 21.620122931764765 27.180133567082837 28.189777866879375 23.009965634273026 96-97 21.64145544544248 27.155206584364482 28.205698822631053 22.997639147561987 98-99 21.533699768254863 27.183878759991053 28.02327479312026 23.259146678633826 100-101 21.555350426232025 27.09967364999264 28.124806654155044 23.220169269620293 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 651.0 1 478.5 2 369.5 3 416.0 4 363.0 5 393.5 6 665.0 7 1489.0 8 2513.5 9 2847.5 10 2371.5 11 1893.0 12 1835.5 13 1944.5 14 2136.0 15 2266.5 16 2324.5 17 2405.0 18 2562.5 19 2800.5 20 3086.0 21 3426.5 22 3987.0 23 4765.0 24 5755.0 25 7126.0 26 8965.5 27 11118.0 28 13019.5 29 15021.0 30 17371.5 31 20097.0 32 22939.5 33 25674.5 34 29019.0 35 31360.5 36 33713.0 37 36566.0 38 38220.0 39 39496.5 40 41131.0 41 43409.0 42 45357.0 43 46984.0 44 49061.0 45 50561.5 46 50607.5 47 51734.0 48 55107.0 49 54991.0 50 51960.5 51 49899.0 52 47729.0 53 46140.0 54 44694.0 55 42167.0 56 38492.5 57 34483.0 58 30770.0 59 27134.0 60 23522.5 61 19061.0 62 14938.0 63 11885.0 64 9114.0 65 6965.5 66 4990.0 67 3680.5 68 2628.0 69 1631.0 70 1106.5 71 747.5 72 515.5 73 385.0 74 301.0 75 205.0 76 119.0 77 69.0 78 46.5 79 35.5 80 29.0 81 18.0 82 16.0 83 15.0 84 16.0 85 13.5 86 9.5 87 7.5 88 7.5 89 8.5 90 5.0 91 2.0 92 2.0 93 2.0 94 2.5 95 2.0 96 1.5 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.052783194626486084 2 0.08432523825254236 3 0.06443385938926363 4 0.11785641976492653 5 0.09782295962405294 6 0.09491029343335855 7 0.09697047195848385 8 0.08837455466399555 9 0.10705824266771806 10-11 0.09871096760902073 12-13 0.08208745813042351 14-15 0.08173225493643639 16-17 0.09434196832297916 18-19 0.07636868670723086 20-21 0.06237368086413833 22-23 0.06521530641603529 24-25 0.05427504804123199 26-27 0.04916012204781745 28-29 0.059958299145025915 30-31 0.04312166775003641 32-33 0.04670922000930632 34-35 0.0410259689055124 36-37 0.045004244678168145 38-39 0.043903114776808076 40-41 0.054949934109807516 42-43 0.05221486951610669 44-45 0.04052868443393043 46-47 0.030654035641088484 48-49 0.03786466047902703 50-51 0.02220019962419502 52-53 0.03306941736020091 54-55 0.04006692028174717 56-57 0.04354791158282095 58-59 0.047561707674875414 60-61 0.045501529149750114 62-63 0.049302203325412304 64-65 0.0351295958853262 66-67 0.05395536516664358 68-69 0.04553704946914883 70-71 0.021773955791410476 72-73 0.01708527363078049 74-75 0.025574629967072667 76-77 0.03910787165798195 78-79 0.05445264963822554 80-81 0.03509407556592749 82-83 0.05445264963822554 84-85 0.057791559661704475 86-87 0.05878612860486841 88-89 0.08045352343808276 90-91 0.05917685211825424 92-93 0.06677820046957862 94-95 0.07182208582419573 96-97 0.08251370196320805 98-99 0.1118534857865442 100-101 0.10556638925297217 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1407645.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.27051065682423 #Duplication Level Percentage of deduplicated Percentage of total 1 78.81260558083353 47.500759845517116 2 12.462587393475339 15.022530126201172 3 3.775703374434332 6.8269071139755475 4 1.6947778337460897 4.085805019589727 5 0.8903115854689857 2.682976694995128 6 0.5319936630562027 1.923811784315509 7 0.350731937067101 1.4797155065483918 8 0.24537637957286143 1.1831167759983272 9 0.1735741697481716 0.9415263462800935 >10 0.9609827064030843 10.462571897414872 >50 0.06966914809341657 2.8576349764059135 >100 0.030272748149875874 3.2204859872443823 >500 8.245299714254462E-4 0.31882791050234355 >1k 3.533699877537627E-4 0.4723139809779763 >5k 2.355799918358418E-4 1.021016034033557 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8503 0.6040585516944968 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5879 0.41764791549005603 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4138 0.29396616334374076 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1478 0.10499806414259276 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 7.104063879742407E-5 0.0 0.0 0.0 6 0.0 7.104063879742407E-5 0.0 0.0 0.0 7 0.0 7.104063879742407E-5 0.0 0.0 0.0 8 0.0 7.104063879742407E-5 0.0 0.0 7.104063879742407E-5 9 0.0 7.104063879742407E-5 0.0 0.0 1.4208127759484813E-4 10-11 0.0 7.104063879742407E-5 0.0 0.0 1.4208127759484813E-4 12-13 0.0 7.104063879742407E-5 0.0 0.0 1.4208127759484813E-4 14-15 0.0 7.104063879742407E-5 0.0 0.0 2.1312191639227221E-4 16-17 0.0 7.104063879742407E-5 0.0 0.0 3.552031939871203E-4 18-19 0.0 7.104063879742407E-5 0.0 0.0 4.972844715819684E-4 20-21 0.0 7.104063879742407E-5 0.0 3.552031939871203E-5 5.328047909806805E-4 22-23 0.0 7.104063879742407E-5 0.0 4.262438327845444E-4 6.038454297781046E-4 24-25 0.0 7.104063879742407E-5 0.0 9.590486237652249E-4 6.393657491768166E-4 26-27 0.0 7.104063879742407E-5 0.0 0.0015984143729420414 6.748860685755287E-4 28-29 0.0 7.104063879742407E-5 0.0 0.003338910023478931 7.104063879742406E-4 30-31 0.0 7.104063879742407E-5 0.0 0.006358137172369454 7.459267073729527E-4 32-33 0.0 7.104063879742407E-5 0.0 0.010904738055404595 7.814470267716647E-4 34-35 0.0 7.104063879742407E-5 0.0 0.015451338938439734 8.524876655690887E-4 36-37 0.0 7.104063879742407E-5 0.0 0.022164679304796306 8.524876655690887E-4 38-39 0.0 7.104063879742407E-5 0.0 0.03193276713944212 8.524876655690887E-4 40-41 0.0 7.104063879742407E-5 0.0 0.04823659374345094 8.524876655690887E-4 42-43 0.0 7.104063879742407E-5 0.0 0.06329720916850484 8.524876655690887E-4 44-45 0.0 7.104063879742407E-5 0.0 0.08166121429763896 8.524876655690887E-4 46-47 0.0 7.104063879742407E-5 0.0 0.10989986821961503 0.0010300892625626488 48-49 0.0 7.104063879742407E-5 0.0 0.13597178265826965 0.001065609581961361 50-51 0.0 7.104063879742407E-5 0.0 0.16328690827587922 0.001065609581961361 52-53 0.0 7.104063879742407E-5 0.0 0.18616199396864974 0.001136650220758785 54-55 0.0 7.104063879742407E-5 0.0 0.21155902233872886 0.0012432111789549213 56-57 0.0 7.104063879742407E-5 0.0 0.2564922263780996 0.0013142518177523452 58-59 0.0 7.104063879742407E-5 0.0 0.29801547975519393 0.0013497721371510572 60-61 0.0 7.104063879742407E-5 0.0 0.33786927812054884 0.0013497721371510572 62-63 0.0 7.104063879742407E-5 0.0 0.38209207577194537 0.0013497721371510572 64-65 0.0 7.104063879742407E-5 0.0 0.43203364484653445 0.0013497721371510572 66-67 0.0 7.104063879742407E-5 0.0 0.48602453033257675 0.0013497721371510572 68-69 0.0 7.104063879742407E-5 0.0 0.5493217395010817 0.0013497721371510572 70-71 0.0 7.104063879742407E-5 0.0 0.626613954512679 0.0013852924565497691 72-73 0.0 7.104063879742407E-5 0.0 0.7029826412199098 0.0017049753311381775 74-75 0.0 7.104063879742407E-5 0.0 0.7895456595945711 0.0017049753311381775 76-77 0.0 7.104063879742407E-5 0.0 0.8889315132721674 0.0017049753311381775 78-79 0.0 7.104063879742407E-5 0.0 0.9973750483964352 0.0017049753311381775 80-81 0.0 7.104063879742407E-5 0.0 1.116439159020918 0.0017049753311381775 82-83 0.0 7.104063879742407E-5 0.0 1.2423231709699534 0.0017049753311381775 84-85 0.0 7.104063879742407E-5 0.0 1.3805682540697406 0.0017049753311381775 86-87 0.0 7.104063879742407E-5 0.0 1.5304640019323053 0.0017049753311381775 88-89 0.0 7.104063879742407E-5 0.0 1.6926142599874257 0.0017049753311381775 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3220 0.0 49.442963 1 GTATCAA 6270 0.0 47.145298 1 TACGGTA 65 1.3254573E-4 36.551937 4 TATCAAC 8170 0.0 36.123104 2 ATCAACG 8185 0.0 35.703453 3 TCAACGC 8220 0.0 35.49362 4 CAACGCA 8325 0.0 35.103027 5 AACGCAG 8520 0.0 34.243847 6 ACGCAGA 9845 0.0 29.345505 7 CGCAGAG 9850 0.0 29.137646 8 CTACGGT 70 0.008377118 27.152866 3 GCAGAGT 10425 0.0 27.12031 9 GTACATG 5770 0.0 25.862432 1 TACACCG 155 1.4064881E-6 24.52517 5 TACATGG 5975 0.0 24.179716 2 ACATGGG 5915 0.0 23.618174 3 AGTACTT 6660 0.0 23.254776 12-13 AGAGTAC 9355 0.0 23.159462 10-11 GTGGTAT 1610 0.0 23.024185 1 TGGTATC 1630 0.0 22.450119 2 >>END_MODULE