FastQCFastQC Report
Thu 26 May 2016
SRR937063_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937063_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407645
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77650.5516305602619979No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65730.4669501188154684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44910.3190435088392315No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16520.11735913529334456No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14730.10464286094860566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56750.037.839391
GTATCAA82900.030.8318311
TATCAAC107250.023.7841052
ATCAACG107050.023.6510493
TCAACGC108650.023.2573854
CAACGCA109100.023.2049945
AACGCAG112850.022.4759836
GCACCGT3700.021.823596
AGTACTT76900.021.37119112-13
CCGTCCA2056.8336885E-720.8529579
GAGTACT74900.020.70524412-13
CGATCGT1301.4881152E-820.1011924-25
AGAGTAC110050.019.89710410-11
ACGCAGA127950.019.7121167
GTACTTT79150.019.53345514-15
CGCAGAG129100.019.4261478
TACTTTT85800.018.9052514-15
GCAGAGT134650.018.4490629
GTACCGT1300.007565416418.2686166
TGGTATC34000.018.0227322
Produced by FastQC (version 0.11.5)