##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937063_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1407645 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.111172206060477 31.0 31.0 33.0 30.0 34.0 2 31.105488954956684 31.0 31.0 34.0 28.0 34.0 3 31.247182350663696 31.0 31.0 34.0 28.0 34.0 4 35.1381321284841 35.0 35.0 37.0 32.0 37.0 5 34.985666130309845 35.0 35.0 37.0 32.0 37.0 6 35.099940681066606 36.0 35.0 37.0 32.0 37.0 7 35.02677166473081 36.0 35.0 37.0 32.0 37.0 8 35.004876939853446 36.0 35.0 37.0 32.0 37.0 9 36.48773447850843 38.0 35.0 39.0 32.0 39.0 10-11 36.33147633103516 38.0 35.0 39.0 32.0 39.0 12-13 36.17271648746666 38.0 35.0 39.0 32.0 39.0 14-15 37.02249963591673 38.0 36.0 40.0 31.0 41.0 16-17 36.86492546060974 38.0 36.0 40.0 31.0 41.0 18-19 36.91304235087682 38.5 36.0 40.0 31.0 41.0 20-21 37.026729395550724 39.0 36.0 40.0 31.0 41.0 22-23 36.9156960739391 39.0 36.0 40.0 31.0 41.0 24-25 36.73750412923713 38.5 35.5 40.0 30.5 41.0 26-27 36.434414927059024 38.0 35.0 40.0 30.0 41.0 28-29 36.14047895598677 38.0 35.0 40.0 29.5 41.0 30-31 35.89035197084492 38.0 34.0 40.0 29.0 41.0 32-33 35.78796536058452 38.0 34.0 40.0 29.0 41.0 34-35 35.5864014009214 38.0 34.0 40.0 28.5 41.0 36-37 35.31220300572943 38.0 34.0 40.0 27.0 41.0 38-39 35.03316638783216 38.0 33.5 40.0 26.5 41.0 40-41 34.70133911604133 38.0 33.0 40.0 25.5 41.0 42-43 34.319104248585404 37.0 33.0 40.0 24.5 41.0 44-45 33.804945138866685 37.0 33.0 40.0 23.5 41.0 46-47 33.364077235382496 37.0 32.0 40.0 22.0 41.0 48-49 32.86248095222872 37.0 31.0 39.0 19.0 40.0 50-51 32.42753250997232 36.0 31.0 39.0 16.5 40.0 52-53 32.02427742790263 36.0 30.0 39.0 14.5 40.0 54-55 31.638051852562256 35.5 30.0 39.0 12.0 40.0 56-57 31.219340458709404 35.0 29.0 38.5 8.5 40.0 58-59 30.678211480877636 35.0 28.0 38.0 8.0 40.0 60-61 30.15198292183043 34.0 28.0 38.0 7.0 40.0 62-63 29.60342806602517 34.0 27.0 38.0 3.5 40.0 64-65 29.050684298953215 34.0 26.0 37.0 2.0 39.0 66-67 28.503112290385715 33.0 26.0 37.0 2.0 39.0 68-69 27.883870578164238 32.5 25.0 36.0 2.0 39.0 70-71 27.235073118577482 32.0 23.5 36.0 2.0 38.5 72-73 26.674213313726117 31.0 22.5 35.0 2.0 38.0 74-75 26.04765903335003 31.0 21.5 35.0 2.0 37.5 76-77 25.18692390481975 29.5 21.0 34.0 2.0 36.0 78-79 25.82426641660362 31.0 22.0 34.0 2.0 36.0 80-81 25.528974279736723 31.0 20.5 34.0 2.0 36.0 82-83 24.995396211402735 31.0 19.0 34.0 2.0 36.0 84-85 24.37637117312959 30.5 17.5 34.0 2.0 35.0 86-87 23.712031087383536 30.0 9.5 34.0 2.0 35.0 88-89 23.021401702844113 29.5 4.5 34.0 2.0 35.0 90-91 22.19499341098075 29.0 2.0 34.0 2.0 35.0 92-93 21.269465667835284 29.0 2.0 33.5 2.0 35.0 94-95 20.280484426115958 27.0 2.0 33.0 2.0 34.5 96-97 19.077701764294265 25.5 2.0 32.5 2.0 34.0 98-99 17.708933360328775 24.0 2.0 32.0 2.0 34.0 100-101 14.892010769760843 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38341912886788165 1101 2 0.35292687356032104 1101 3 0.3086181283859659 1101 4 0.44053195708519155 1101 5 0.4658762599492974 1101 6 0.3165101744951997 1101 7 0.4634440787666634 1101 8 0.46161678111882054 1101 9 0.5680306634425492 1101 10-11 0.5824097207517696 1101 12-13 0.5066607502063789 1101 14-15 0.564104314774788 1101 16-17 0.6058775924633792 1101 18-19 0.6350536946254124 1101 20-21 0.7452254833041394 1101 22-23 0.5604305420672091 1101 24-25 0.6729918461972204 1101 26-27 0.7409894248955595 1101 28-29 0.7942095690749511 1101 30-31 0.6415341199873339 1101 32-33 0.7830215801030249 1101 34-35 0.9241551369988983 1101 36-37 1.0141183060214374 1101 38-39 0.9434379506933794 1101 40-41 0.9200625632540635 1101 42-43 1.240334832284539 1101 44-45 1.3877453302351839 1101 46-47 1.6464729649093286 1101 48-49 1.3165687867060072 1101 50-51 1.195657691090716 1101 52-53 0.9497105268223578 1101 54-55 1.1814683374315003 1101 56-57 1.3100332532802348 1101 58-59 1.3127703944786013 1101 60-61 1.5078639292953433 1101 62-63 1.6689158351517897 1101 64-65 1.3913387591598294 1101 66-67 1.2876884790530916 1101 68-69 1.3163515431396142 1101 70-71 1.4162668087787296 1101 72-73 1.5280997563056395 1101 74-75 1.2264497803748284 1101 76-77 1.046417263336263 1101 78-79 1.3010896231231825 1101 80-81 1.635807994597105 1101 82-83 1.6110902887103613 1101 84-85 1.7083111167433565 1101 86-87 1.3863417671675649 1101 88-89 1.588499537619949 1101 90-91 1.828622469735432 1101 92-93 1.8346197371718844 1101 94-95 1.638338146514048 1101 96-97 1.6901332273158722 1101 98-99 1.5194355847455867 1101 100-101 1.283081224829262 1102 1 0.21797258123203633 1102 2 0.032164331404203494 1102 3 0.2073143039556733 1102 4 0.25573285004391977 1102 5 0.32076762118796864 1102 6 0.32495505181577755 1102 7 0.42185231838180925 1102 8 0.3889421375968638 1102 9 0.44097121478312573 1102 10-11 0.3546881764155998 1102 12-13 0.359020753210217 1102 14-15 0.47656437740022994 1102 16-17 0.5789468877906003 1102 18-19 0.5383210497471822 1102 20-21 0.4405212360610733 1102 22-23 0.41247764541284226 1102 24-25 0.5053951090010713 1102 26-27 0.43917978394507173 1102 28-29 0.4174789495108726 1102 30-31 0.2820072925395465 1102 32-33 0.594403959414791 1102 34-35 0.4333998996275241 1102 36-37 0.6622319193184154 1102 38-39 0.7873573431901661 1102 40-41 0.7161723102490853 1102 42-43 0.7971508677662271 1102 44-45 0.8685725459061118 1102 46-47 0.9271456650399443 1102 48-49 0.8772056114685398 1102 50-51 0.943171561079204 1102 52-53 1.1704751739398134 1102 54-55 1.1478970486490105 1102 56-57 1.0859956757380154 1102 58-59 1.0766770775601735 1102 60-61 1.0858331194882744 1102 62-63 1.3239655805083608 1102 64-65 1.1845712515705884 1102 66-67 1.1551791717105324 1102 68-69 1.1368842462740716 1102 70-71 0.9960582127993227 1102 72-73 1.1287706700636662 1102 74-75 0.7683345286214518 1102 76-77 0.9741533717292583 1102 78-79 1.3301682417223937 1102 80-81 1.1832097327262652 1102 82-83 1.3423871383228203 1102 84-85 1.2520155187295323 1102 86-87 1.0609924004764721 1102 88-89 1.31479056160968 1102 90-91 1.5504592248635696 1102 92-93 1.618758227698418 1102 94-95 1.5822798971136791 1102 96-97 1.7876984697391514 1102 98-99 1.7125544092441523 1102 100-101 1.273357156815118 1103 1 0.030434061414297275 1103 2 -0.17688234838047734 1103 3 0.07861209673300706 1103 4 0.20922347288043142 1103 5 0.2468149149147152 1103 6 0.2513490981215014 1103 7 0.27222145550226884 1103 8 0.3250755375890293 1103 9 0.26943200146023116 1103 10-11 0.18293046146759195 1103 12-13 0.3337508621403984 1103 14-15 0.26643112703417415 1103 16-17 0.33706262002635157 1103 18-19 0.36950716919394466 1103 20-21 0.2610666826178374 1103 22-23 0.3844928122756457 1103 24-25 0.48101436516928686 1103 26-27 0.5191786914369416 1103 28-29 0.5391217136039259 1103 30-31 0.563815310361683 1103 32-33 0.6664370576609571 1103 34-35 0.6498759315430149 1103 36-37 0.5137187991270835 1103 38-39 0.5809902770851068 1103 40-41 0.5882618745402226 1103 42-43 0.6393704286492081 1103 44-45 0.9239478640100387 1103 46-47 0.9861553539245094 1103 48-49 0.7852351305630876 1103 50-51 0.5568948287710072 1103 52-53 0.6520355367950117 1103 54-55 0.7573770504065287 1103 56-57 0.8173304119151119 1103 58-59 0.8086020056268275 1103 60-61 0.7922248264015934 1103 62-63 0.6788145946979043 1103 64-65 0.35673004762663396 1103 66-67 0.8438876846050931 1103 68-69 1.0373653654773953 1103 70-71 0.9318436727036783 1103 72-73 0.9932708654088707 1103 74-75 0.8369918361351765 1103 76-77 0.876550536433168 1103 78-79 0.931709153182922 1103 80-81 1.129008267102865 1103 82-83 1.1536023279857943 1103 84-85 1.1392826334036954 1103 86-87 0.7675515461351203 1103 88-89 1.1978037227700646 1103 90-91 1.7155469995788941 1103 92-93 1.632414579267536 1103 94-95 1.420854102759332 1103 96-97 1.7778195837649342 1103 98-99 1.7000493474877523 1103 100-101 1.6491223578187988 1104 1 0.13130516144264348 1104 2 0.06143104495883378 1104 3 0.14614191051949987 1104 4 0.17354954726064875 1104 5 0.17064657493044422 1104 6 0.09732370781969024 1104 7 0.27080019378615816 1104 8 0.3119134739888523 1104 9 0.3143293768721449 1104 10-11 0.26461773514641607 1104 12-13 0.3380672391031325 1104 14-15 0.37655453821621876 1104 16-17 0.24933209791790745 1104 18-19 0.3623174833466578 1104 20-21 0.2740789878196779 1104 22-23 0.33179421083309535 1104 24-25 0.27408834389397185 1104 26-27 0.1985321712833823 1104 28-29 0.3182553340116101 1104 30-31 0.2915907792305319 1104 32-33 0.4521998121379909 1104 34-35 0.28504747706251266 1104 36-37 0.27251702485884977 1104 38-39 0.05849124233731118 1104 40-41 0.339524398767729 1104 42-43 0.5080820189872028 1104 44-45 0.38349834509686787 1104 46-47 0.5207011209889458 1104 48-49 0.617891183945062 1104 50-51 0.5530533815468885 1104 52-53 0.39252186997826755 1104 54-55 0.43652465254047 1104 56-57 0.536241594602398 1104 58-59 0.3936330283836931 1104 60-61 0.4870140037758226 1104 62-63 0.30790857883570055 1104 64-65 0.32681973591110136 1104 66-67 0.5279436422956216 1104 68-69 0.43886763141495777 1104 70-71 0.4857134040621247 1104 72-73 0.55517175164508 1104 74-75 0.5401963451777618 1104 76-77 0.5090639505526617 1104 78-79 0.6900725344004606 1104 80-81 0.8047874276605427 1104 82-83 0.7400174133705271 1104 84-85 0.8028303489307227 1104 86-87 0.8286215458400932 1104 88-89 0.857566110387129 1104 90-91 1.0755940884936948 1104 92-93 1.3135728562570783 1104 94-95 0.916054779013102 1104 96-97 1.0653037581183433 1104 98-99 0.676204090563914 1104 100-101 0.35220212164307085 1105 1 0.12434450744104453 1105 2 0.009417277641624366 1105 3 0.1078225174053209 1105 4 0.17818277901408663 1105 5 0.025572389190529066 1105 6 0.15592530932393345 1105 7 0.16574537846704374 1105 8 0.2200005571330692 1105 9 0.24798703642822062 1105 10-11 0.3543092672737629 1105 12-13 0.43779383790578663 1105 14-15 0.44898998365485454 1105 16-17 0.4344834006259859 1105 18-19 0.3286789375600634 1105 20-21 0.2763932398026441 1105 22-23 0.2878528029790175 1105 24-25 0.24263943911972063 1105 26-27 0.43638986217792564 1105 28-29 0.4611891828088588 1105 30-31 0.49258354706645235 1105 32-33 0.46854140964388336 1105 34-35 0.5540011771640891 1105 36-37 0.5470701138601228 1105 38-39 0.6353019174113399 1105 40-41 0.07401354106603009 1105 42-43 0.26775101983009364 1105 44-45 0.46026511928693026 1105 46-47 0.2897892253352765 1105 48-49 0.3809540608172668 1105 50-51 0.4506353208583107 1105 52-53 0.5193707725912446 1105 54-55 0.3971361040522794 1105 56-57 0.6576120045961353 1105 58-59 0.6257714471954223 1105 60-61 0.7930970453371309 1105 62-63 0.6944518097246295 1105 64-65 0.4268198709907445 1105 66-67 0.38431447103624805 1105 68-69 0.08744004220836388 1105 70-71 -0.032482836941571946 1105 72-73 0.5481368038398529 1105 74-75 0.4969499220867064 1105 76-77 0.3880947270985118 1105 78-79 0.6279683177543269 1105 80-81 0.9505285691375569 1105 82-83 1.0865956433949542 1105 84-85 1.083266831781014 1105 86-87 1.0497823797677768 1105 88-89 1.178125974983299 1105 90-91 1.6899396816554209 1105 92-93 1.4874737132023306 1105 94-95 1.2518658106973852 1105 96-97 1.3560221656914493 1105 98-99 1.4229702839115106 1105 100-101 1.1835703838704799 1106 1 0.39654634764603003 1106 2 0.23092321754378986 1106 3 0.297293749643849 1106 4 0.2805878570480971 1106 5 0.25366172066036796 1106 6 0.2791870144252613 1106 7 0.33981508496599844 1106 8 0.29466134213191 1106 9 0.4161430462115874 1106 10-11 0.39937973081906364 1106 12-13 0.4079953287047573 1106 14-15 0.5351615128884859 1106 16-17 0.6719011446278458 1106 18-19 0.505867820625518 1106 20-21 0.38644375808822673 1106 22-23 0.45963244425551864 1106 24-25 0.4718228481576858 1106 26-27 0.4425597899673548 1106 28-29 0.6009268371298404 1106 30-31 0.40243461275478154 1106 32-33 0.5756088741209382 1106 34-35 0.36213796961692424 1106 36-37 0.2713429126022646 1106 38-39 0.31986326728321757 1106 40-41 0.32219590188596925 1106 42-43 0.193257658544276 1106 44-45 0.4985199818538959 1106 46-47 0.416195584524651 1106 48-49 0.4777613634028697 1106 50-51 0.250036963066556 1106 52-53 0.3301157353212716 1106 54-55 0.60517680985242 1106 56-57 0.3493572876150033 1106 58-59 0.44653489932679946 1106 60-61 0.5868994562385943 1106 62-63 0.4474115697991756 1106 64-65 0.42543243796023233 1106 66-67 0.6138574770926191 1106 68-69 0.8560815807594935 1106 70-71 0.8065101458210684 1106 72-73 1.1078095268773573 1106 74-75 0.9482878559324988 1106 76-77 0.769500503944542 1106 78-79 1.0530673161237658 1106 80-81 1.0185935877725427 1106 82-83 1.1644542213124929 1106 84-85 0.9288199123773317 1106 86-87 1.0070929725933055 1106 88-89 1.2522619230382688 1106 90-91 1.567855765978738 1106 92-93 1.7702261590444017 1106 94-95 1.6281840904551288 1106 96-97 1.781913151016397 1106 98-99 1.539660244312163 1106 100-101 1.2533635063955213 1107 1 0.13793686096636648 1107 2 0.06368349843343069 1107 3 0.16040623858597058 1107 4 0.23281391527711293 1107 5 0.1673143819879357 1107 6 0.17593658581021998 1107 7 0.07167233854170263 1107 8 0.043892575055807015 1107 9 0.3386247860363838 1107 10-11 0.14462719240028576 1107 12-13 0.18861938438165993 1107 14-15 0.10520053838619248 1107 16-17 0.17319775406755866 1107 18-19 0.2943071530037642 1107 20-21 0.15830625681608268 1107 22-23 0.3355714830618126 1107 24-25 0.3210317696134979 1107 26-27 0.4070448256265635 1107 28-29 0.34179714095724734 1107 30-31 0.43661108472452526 1107 32-33 0.48434811960188995 1107 34-35 0.3523408865478004 1107 36-37 0.35323363338010694 1107 38-39 0.5985836067306778 1107 40-41 0.42418788541463215 1107 42-43 0.4524176904849142 1107 44-45 0.3929301762376767 1107 46-47 0.5896308460385384 1107 48-49 0.5133716407696483 1107 50-51 0.49366880035724137 1107 52-53 0.4022547788247195 1107 54-55 0.38141744490726026 1107 56-57 0.3153369959219141 1107 58-59 0.21948517998158223 1107 60-61 0.2779302460645674 1107 62-63 0.38144145150925013 1107 64-65 0.5687910576240682 1107 66-67 0.2953238885474079 1107 68-69 0.5089617172900205 1107 70-71 0.40688043790558126 1107 72-73 0.293048925015718 1107 74-75 0.4491695503515025 1107 76-77 0.633842863636719 1107 78-79 0.6479728642421669 1107 80-81 0.6597831021653988 1107 82-83 0.5090172688241772 1107 84-85 0.6007736141238915 1107 86-87 0.7651466297169236 1107 88-89 0.7116834297277954 1107 90-91 0.8697753350934043 1107 92-93 1.0435304874692086 1107 94-95 1.1213559983596504 1107 96-97 1.227882995305837 1107 98-99 1.1444934171594987 1107 100-101 0.6232686630846569 1108 1 -0.048379636396745695 1108 2 -0.08990420032464996 1108 3 -0.049477859445627104 1108 4 0.0038146705013204496 1108 5 -0.04612366212210617 1108 6 0.02554931485615697 1108 7 0.04703693357991057 1108 8 -0.07481195317640044 1108 9 -0.016548926191212843 1108 10-11 0.09399582752550373 1108 12-13 -0.16282980600179542 1108 14-15 -0.18793391497604972 1108 16-17 0.026915175695457094 1108 18-19 0.033370581175795166 1108 20-21 -0.1105266936457312 1108 22-23 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-1.031724327759246 2316 68-69 -1.0614190411066282 2316 70-71 -0.9925419992897595 2316 72-73 -0.8993311326934936 2316 74-75 -0.9238711977836402 2316 76-77 -1.225740583732037 2316 78-79 -0.9871700403560801 2316 80-81 -1.0357722397021583 2316 82-83 -1.0441452052614864 2316 84-85 -1.1636790729989954 2316 86-87 -0.9846067511672167 2316 88-89 -1.0836717925790786 2316 90-91 -1.1770063106613513 2316 92-93 -1.398739121188111 2316 94-95 -1.0067975153995796 2316 96-97 -0.774602696750776 2316 98-99 -0.4192059300983715 2316 100-101 -0.23804263109580504 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 22.0 4 52.0 5 88.0 6 231.0 7 632.0 8 1398.0 9 2240.0 10 3672.0 11 5495.0 12 7738.0 13 10332.0 14 13570.0 15 17344.0 16 21781.0 17 26567.0 18 23973.0 19 19943.0 20 19900.0 21 20805.0 22 22499.0 23 24593.0 24 28263.0 25 32266.0 26 38591.0 27 45321.0 28 52848.0 29 61898.0 30 70959.0 31 82499.0 32 94398.0 33 108894.0 34 125121.0 35 140394.0 36 142751.0 37 107721.0 38 31965.0 39 873.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.734488325034675 23.034752063297184 16.258412341131802 19.972347270536346 2 18.3330006230326 24.321004581456364 36.406913459564336 20.939081335946707 3 22.26560529690292 25.907155010425438 30.59103519073042 21.236204501941227 4 16.45515485449446 19.943821022929093 35.189508142683806 28.41151597989264 5 13.808009945830744 38.65781014119528 33.581031879939616 13.953148033034365 6 30.818270877689518 38.665453287384054 16.352132897710277 14.164142937216148 7 27.23605738662802 34.30318013419576 20.692930390830075 17.76783208834614 8 26.39060274429988 35.89044112684661 19.180546231471713 18.538409897381797 9 26.33668289945263 16.72531071399394 20.237559896138585 36.70044649041484 10-11 25.026906641944525 27.738598865481002 26.82213910467483 20.412355387899648 12-13 26.120619165310377 25.100079068680348 27.813420647510682 20.96588111849859 14-15 22.98814686941665 26.17307630830216 26.548490564027148 24.290286258254035 16-17 21.648036259142042 29.509997193894765 27.057283619094303 21.784682927868886 18-19 21.665902979799593 27.445094466289444 30.29123109875004 20.597771455160924 20-21 22.437640462808414 26.383368848275126 30.65816249870793 20.520828190208533 22-23 22.243925137374834 26.545329255600663 30.540477179970804 20.670268427053696 24-25 22.05590711866454 26.73783969551063 30.568750765930012 20.637502419894822 26-27 21.94360852045717 26.831916761862544 30.41426545725279 20.810209260427502 28-29 21.668043115712297 26.879616654500634 30.62899281227189 20.82334741751518 30-31 21.897244153929503 26.64898709557093 30.55864721084931 20.89512153965026 32-33 21.85360222358384 26.84640044045431 30.428750299704294 20.87124703625756 34-35 22.184558080982995 26.800730170469016 30.26740593052156 20.74730581802643 36-37 22.330803724932437 26.60841605119651 30.206216840627565 20.854563383243487 38-39 22.04022044340881 26.84504135533978 30.280100950719923 20.834637250531483 40-41 22.227830637386713 26.724966556716467 30.194756064725453 20.852446741171363 42-43 22.17607271265104 26.998706329748806 29.738822113591496 21.08639884400866 44-45 22.345071918399416 27.022094474640834 29.448678885252377 21.184154721707372 46-47 22.546238575777274 27.373805185256227 28.940499912975216 21.139456325991283 48-49 22.675209552422025 27.585296840178934 28.507760845612136 21.2317327617869 50-51 22.511089451594295 27.795095919138646 28.296962868452535 21.396851760814524 52-53 22.81923845531418 27.90868255284203 28.050906517935353 21.22117247390844 54-55 22.564607877056595 27.810603385746163 28.09385005900084 21.530938678196403 56-57 22.582477322932032 28.046427915571016 27.99027027065427 21.38082449084268 58-59 22.821163485911093 27.782048148948018 28.047103220159812 21.349685144981077 60-61 22.64877571593285 27.821941563142254 28.33915513849787 21.190127582427028 62-63 22.51014993126818 27.68833761353182 28.47134753435703 21.330164920842968 64-65 22.471002946059475 27.76495887096373 28.271195182301195 21.492843000675595 66-67 22.53867274774535 28.057287171126244 27.904159074198393 21.499881006930014 68-69 22.425469489821655 27.943586628730966 27.939217629444922 21.691726252002457 70-71 22.55464540560163 27.901453741661154 27.889838584840547 21.654062267896666 72-73 22.717517555917862 27.897658855748432 27.753552919947854 21.63127066838585 74-75 22.60994844551543 28.157792101230644 27.449619468655627 21.7826399845983 76-77 22.64857868287159 28.029933832419985 27.627060366372604 21.69442711833582 78-79 22.604922769808386 28.036664138799445 27.58456071054973 21.77385238084244 80-81 22.455566275126067 28.23873509699475 27.53740048678533 21.76829814109386 82-83 22.590815510281793 28.214223299522462 27.513721606610808 21.681239583584937 84-85 22.564061396071157 28.169289247872907 27.406012086405763 21.860637269650177 86-87 22.651762181823347 28.012261744918764 27.418355675852318 21.917620397405567 88-89 22.6691900516683 27.949401584077666 27.321041777224604 22.06036658702943 90-91 22.545836357003743 28.146295564182267 27.351882774296193 21.9559853045178 92-93 22.617918580323874 28.073519957091452 27.183380752959728 22.125180709624942 94-95 22.52338749122203 27.975584727240953 27.17584388376745 22.32518389776957 96-97 22.658624329903937 27.989591044262923 27.035472288961476 22.316312336871658 98-99 22.481679175373742 28.09200213552029 26.87317133643005 22.553147352675918 100-101 22.558782868891008 28.18363101877045 26.723525508689022 22.534060603649518 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 678.0 1 481.5 2 307.0 3 351.5 4 334.5 5 351.0 6 584.5 7 1201.0 8 1942.0 9 2309.5 10 2085.0 11 1753.0 12 1830.0 13 2041.5 14 2260.0 15 2467.5 16 2676.5 17 2922.0 18 3192.5 19 3450.5 20 3709.0 21 4157.5 22 4820.0 23 5592.0 24 6625.0 25 8073.5 26 9990.0 27 12066.0 28 13967.5 29 15971.5 30 18273.0 31 21115.0 32 24017.5 33 26718.0 34 29717.0 35 32041.0 36 34662.5 37 37781.0 38 39592.5 39 40547.0 40 41830.0 41 43778.5 42 45880.0 43 47477.0 44 48950.0 45 50215.0 46 50325.0 47 51467.0 48 54087.5 49 53889.0 50 51127.0 51 48594.0 52 46309.5 53 44460.5 54 42747.0 55 39780.5 56 36090.0 57 32689.0 58 29267.0 59 26055.0 60 22559.0 61 18543.5 62 14828.5 63 11613.0 64 8884.5 65 6685.5 66 4975.0 67 3834.5 68 2696.5 69 1710.0 70 1193.0 71 843.5 72 592.5 73 430.5 74 316.0 75 218.0 76 141.0 77 89.0 78 60.0 79 37.0 80 26.5 81 14.0 82 9.5 83 5.5 84 1.0 85 0.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013426680732713149 2 9.945689431639369E-4 3 0.002131219163922722 4 7.104063879742407E-5 5 0.0014208127759484813 6 4.262438327845444E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.683251103793925E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0015273737341446173 22-23 0.0 24-25 0.003019227148890523 26-27 0.0014918534147459054 28-29 0.002664023954903402 30-31 0.012964916580529893 32-33 5.328047909806805E-4 34-35 0.002024658205726586 36-37 0.0034809913010737793 38-39 0.0019536175669291615 40-41 0.0026995442743021143 42-43 0.0015628940535433295 44-45 5.683251103793925E-4 46-47 0.0 48-49 1.4208127759484813E-4 50-51 7.104063879742407E-5 52-53 4.262438327845444E-4 54-55 0.0026995442743021143 56-57 3.552031939871203E-5 58-59 9.23528304366513E-4 60-61 4.262438327845444E-4 62-63 0.0 64-65 1.4208127759484813E-4 66-67 0.0 68-69 0.0 70-71 1.065609581961361E-4 72-73 0.0 74-75 5.683251103793925E-4 76-77 4.972844715819684E-4 78-79 1.7760159699356016E-4 80-81 0.0030547474682892347 82-83 7.814470267716647E-4 84-85 0.002166739483321434 86-87 0.001065609581961361 88-89 8.524876655690887E-4 90-91 1.065609581961361E-4 92-93 0.0 94-95 1.065609581961361E-4 96-97 7.814470267716647E-4 98-99 0.0018115362893343138 100-101 4.972844715819684E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1407645.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.59815719712466 #Duplication Level Percentage of deduplicated Percentage of total 1 80.77106004135985 50.56119513447438 2 11.43916598018439 14.32141420463166 3 3.414415056111242 6.412082712560423 4 1.4453353744023738 3.6190132387761933 5 0.7889180473494994 2.4692407971816306 6 0.4735180326531442 1.7784813746216839 7 0.31121610372934994 1.3637088208468553 8 0.22369373057381484 1.120225224837673 9 0.1577678047714878 0.888837645935778 >10 0.8914461452492332 10.165141955137763 >50 0.05484806691492573 2.3648899704942874 >100 0.0271394508755825 2.9870130604515635 >500 7.948585211445573E-4 0.3077095170031069 >1k 4.542048692254613E-4 0.6218874022315043 >5k 2.2710243461273065E-4 1.019158940815557 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7765 0.5516305602619979 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6573 0.4669501188154684 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4491 0.3190435088392315 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1652 0.11735913529334456 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1473 0.10464286094860566 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 3.552031939871203E-5 18-19 0.0 0.0 0.0 0.0 7.104063879742407E-5 20-21 0.0 0.0 0.0 3.552031939871203E-5 7.104063879742407E-5 22-23 0.0 0.0 0.0 4.972844715819684E-4 7.104063879742407E-5 24-25 0.0 0.0 0.0 9.590486237652249E-4 7.104063879742407E-5 26-27 0.0 0.0 0.0 0.0015984143729420414 1.4208127759484813E-4 28-29 0.0 0.0 0.0 0.003552031939871203 1.4208127759484813E-4 30-31 0.0 0.0 0.0 0.006642299727559151 1.4208127759484813E-4 32-33 0.0 0.0 0.0 0.011473063165783986 1.4208127759484813E-4 34-35 0.0 0.0 0.0 0.015913103090622993 1.4208127759484813E-4 36-37 0.0 0.0 0.0 0.022768524734574414 1.4208127759484813E-4 38-39 0.0 0.0 0.0 0.03264317352741636 1.4208127759484813E-4 40-41 0.0 0.0 0.0 0.049515325241804575 1.4208127759484813E-4 42-43 0.0 0.0 0.0 0.0649666641802443 1.4208127759484813E-4 44-45 0.0 0.0 0.0 0.08325962867058101 1.4208127759484813E-4 46-47 0.0 0.0 0.0 0.11117859971796866 1.4208127759484813E-4 48-49 0.0 0.0 0.0 0.13703739224023104 1.4208127759484813E-4 50-51 0.0 0.0 0.0 0.1638552333862586 1.4208127759484813E-4 52-53 0.0 0.0 0.0 0.18633959556564333 1.7760159699356016E-4 54-55 0.0 0.0 0.0 0.21127485978353916 2.131219163922722E-4 56-57 0.0 0.0 0.0 0.2546451697693666 2.486422357909842E-4 58-59 0.0 0.0 0.0 0.29478313068991113 2.8416255518969627E-4 60-61 0.0 0.0 0.0 0.33154666126757815 2.8416255518969627E-4 62-63 0.0 0.0 0.0 0.37186222378511624 2.8416255518969627E-4 64-65 0.0 0.0 0.0 0.41835832187803035 2.8416255518969627E-4 66-67 0.0 0.0 0.0 0.4678026064810375 2.8416255518969627E-4 68-69 0.0 0.0 0.0 0.5258783286979316 2.8416255518969627E-4 70-71 0.0 0.0 0.0 0.597878016119121 2.8416255518969627E-4 72-73 0.0 0.0 0.0 0.6678885656539824 2.8416255518969627E-4 74-75 0.0 0.0 0.0 0.7488038532442484 2.8416255518969627E-4 76-77 0.0 0.0 0.0 0.84087252112571 2.8416255518969627E-4 78-79 0.0 0.0 0.0 0.9410753421494766 2.8416255518969627E-4 80-81 0.0 0.0 0.0 1.050833129091497 2.8416255518969627E-4 82-83 0.0 0.0 0.0 1.1643205495703817 2.8416255518969627E-4 84-85 0.0 0.0 0.0 1.2884285455494817 2.8416255518969627E-4 86-87 0.0 0.0 0.0 1.4206351743514878 2.8416255518969627E-4 88-89 0.0 0.0 0.0 1.5606207531018117 3.552031939871203E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5675 0.0 37.83939 1 GTATCAA 8290 0.0 30.831831 1 TATCAAC 10725 0.0 23.784105 2 ATCAACG 10705 0.0 23.651049 3 TCAACGC 10865 0.0 23.257385 4 CAACGCA 10910 0.0 23.204994 5 AACGCAG 11285 0.0 22.475983 6 GCACCGT 370 0.0 21.82359 6 AGTACTT 7690 0.0 21.371191 12-13 CCGTCCA 205 6.8336885E-7 20.852957 9 GAGTACT 7490 0.0 20.705244 12-13 CGATCGT 130 1.4881152E-8 20.10119 24-25 AGAGTAC 11005 0.0 19.897104 10-11 ACGCAGA 12795 0.0 19.712116 7 GTACTTT 7915 0.0 19.533455 14-15 CGCAGAG 12910 0.0 19.426147 8 TACTTTT 8580 0.0 18.90525 14-15 GCAGAGT 13465 0.0 18.449062 9 GTACCGT 130 0.0075654164 18.268616 6 TGGTATC 3400 0.0 18.022732 2 >>END_MODULE