FastQCFastQC Report
Thu 26 May 2016
SRR937062_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937062_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354462
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83090.613453902730383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58890.43478517669746364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39060.2883801834233814No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13790.10181164181793213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35200.046.7047921
GTATCAA63700.042.6662641
TATCAAC79600.034.0852972
ATCAACG79600.033.9062163
TCAACGC80650.033.4647834
CAACGCA81600.033.1346365
AACGCAG84000.032.2421236
ACGCAGA95350.028.2038047
CGCAGAG96350.027.5658918
CCCGTAC1056.890463E-527.152263
GCAGAGT101400.025.818189
TCGCGCA1301.0001626E-525.5838959
GTACATG53250.024.716571
GTATAGG4100.024.3367981
GAGTACT55850.023.77554512-13
TACATGG57300.022.8875082
AGAGTAC87000.022.17229710-11
ACATGGG57250.021.7455233
GTACTTT62500.021.3214514-15
CAGAGTA96200.021.31128510-11
Produced by FastQC (version 0.11.5)