##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937062_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1354462 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30778050620837 33.0 31.0 34.0 31.0 34.0 2 32.45895565914732 34.0 31.0 34.0 31.0 34.0 3 32.48192197344776 34.0 31.0 34.0 31.0 34.0 4 36.03747170463254 37.0 35.0 37.0 35.0 37.0 5 36.04307540558539 37.0 35.0 37.0 35.0 37.0 6 36.12192073310288 37.0 37.0 37.0 35.0 37.0 7 36.086911999007725 37.0 37.0 37.0 35.0 37.0 8 36.05059573469023 37.0 37.0 37.0 35.0 37.0 9 37.78752227821821 39.0 38.0 39.0 35.0 39.0 10-11 37.64266328623468 39.0 38.0 39.0 35.0 39.0 12-13 37.59141969283745 39.0 38.0 39.0 35.0 39.0 14-15 38.889744784275976 40.0 38.0 41.0 36.0 41.0 16-17 38.828614977755 40.0 38.0 41.0 36.0 41.0 18-19 38.799479793453045 40.0 38.0 41.0 36.0 41.0 20-21 38.73452706683539 40.0 38.0 41.0 35.0 41.0 22-23 38.632376545078415 40.0 38.0 41.0 35.0 41.0 24-25 38.496369776339236 40.0 38.0 41.0 34.5 41.0 26-27 38.58912874632142 40.0 38.0 41.0 35.0 41.0 28-29 38.543427574933816 40.0 38.0 41.0 35.0 41.0 30-31 38.38738259175968 40.0 38.0 41.0 34.5 41.0 32-33 38.1901149681571 40.0 38.0 41.0 34.0 41.0 34-35 38.031861728125264 40.0 38.0 41.0 34.0 41.0 36-37 37.863670593933236 40.0 38.0 41.0 34.0 41.0 38-39 37.70935507972908 40.0 37.0 41.0 33.0 41.0 40-41 37.533611500359555 40.0 37.0 41.0 33.0 41.0 42-43 37.3123627683907 40.0 37.0 41.0 33.0 41.0 44-45 37.044682316668904 40.0 37.0 41.0 32.0 41.0 46-47 36.660218965168454 39.5 36.0 41.0 31.0 41.0 48-49 36.316333717741806 39.0 36.0 41.0 30.0 41.0 50-51 35.69097877976643 38.5 35.5 40.0 29.5 40.5 52-53 35.9303505746193 39.0 36.0 40.0 30.5 40.5 54-55 36.296315437420915 39.0 36.0 41.0 31.0 41.0 56-57 36.189987242167 39.0 36.0 41.0 30.5 41.0 58-59 35.96017053265429 39.0 35.0 41.0 30.0 41.0 60-61 35.69166355349947 39.0 35.0 40.5 29.0 41.0 62-63 35.37829521979945 38.0 35.0 40.0 29.0 41.0 64-65 35.06724662633577 38.0 35.0 40.0 28.0 41.0 66-67 34.70547974029541 37.0 34.5 40.0 28.0 41.0 68-69 34.32509845237445 37.0 34.0 39.0 27.5 41.0 70-71 33.909604329984894 36.0 34.0 39.0 26.0 41.0 72-73 33.52180718248279 36.0 34.0 39.0 26.0 40.0 74-75 33.103862271514444 35.0 33.5 37.5 26.0 40.0 76-77 32.684177924519105 35.0 33.0 37.0 25.0 39.0 78-79 32.25682632661529 35.0 33.0 36.5 24.5 39.0 80-81 31.83668644819862 35.0 32.5 36.0 23.0 38.5 82-83 31.452836255280694 35.0 32.0 36.0 21.5 37.0 84-85 31.121756461237005 35.0 32.0 35.0 20.0 37.0 86-87 30.810795725535307 34.5 31.5 35.0 19.5 36.5 88-89 30.489601406314833 34.0 31.0 35.0 17.5 36.0 90-91 30.16658126990643 34.0 31.0 35.0 12.5 36.0 92-93 29.83128873309107 34.0 31.0 35.0 7.0 35.0 94-95 29.46716925244119 34.0 30.0 35.0 2.0 35.0 96-97 29.05426988723198 34.0 30.0 35.0 2.0 35.0 98-99 28.519334983188898 34.0 29.5 35.0 2.0 35.0 100-101 26.277254363725227 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29734971449545355 1101 2 0.31387107649392476 1101 3 0.35630405528637255 1101 4 0.2670003955960638 1101 5 0.21169954495869092 1101 6 0.24789946147005537 1101 7 0.20378147308568373 1101 8 0.18495543080712906 1101 9 0.22031512765231298 1101 10-11 0.26173462368321765 1101 12-13 0.26348242689640955 1101 14-15 0.5554237401257112 1101 16-17 0.6176292446971416 1101 18-19 0.568364963617185 1101 20-21 0.5104019417157275 1101 22-23 0.6198023928951031 1101 24-25 0.5167073192755467 1101 26-27 0.5599269677689236 1101 28-29 0.5502220206073716 1101 30-31 0.5388961844903477 1101 32-33 0.5800161963901118 1101 34-35 0.594325193196731 1101 36-37 0.5366849168413523 1101 38-39 0.5436786793400827 1101 40-41 0.5577630480271907 1101 42-43 0.5336813076965541 1101 44-45 0.6806636389241945 1101 46-47 0.6710713770198282 1101 48-49 0.8880715618728701 1101 50-51 0.6672006302731361 1101 52-53 0.7962029823774017 1101 54-55 0.7092429644340328 1101 56-57 0.6626421997627432 1101 58-59 0.6037892302744865 1101 60-61 0.7469333458144263 1101 62-63 0.7529662970605031 1101 64-65 0.7730305437780629 1101 66-67 0.8047350858406261 1101 68-69 0.8177496235075594 1101 70-71 0.7524072276887352 1101 72-73 0.8429441439028054 1101 74-75 0.753988378528355 1101 76-77 0.8398420556511397 1101 78-79 0.8810602911753946 1101 80-81 0.7858918278658358 1101 82-83 0.9136299469605724 1101 84-85 0.9185003091529218 1101 86-87 0.8896346861364179 1101 88-89 0.7490139134037435 1101 90-91 0.9724204953992981 1101 92-93 0.9757362967636816 1101 94-95 0.9486626360868762 1101 96-97 0.8858520420362943 1101 98-99 0.9311233894162605 1101 100-101 0.8836665421461127 1102 1 0.3122021663476531 1102 2 0.24464169022451188 1102 3 0.16456254736061737 1102 4 0.18765326399622495 1102 5 0.1759381002619378 1102 6 0.16817478141816622 1102 7 0.1629238157669164 1102 8 0.13757766384718195 1102 9 0.33409163556423493 1102 10-11 0.39942474629656743 1102 12-13 0.2956084810587143 1102 14-15 0.32018726756228233 1102 16-17 0.3573586821361161 1102 18-19 0.2582201122440466 1102 20-21 0.3103045812357834 1102 22-23 0.3330589073346033 1102 24-25 0.397109373802941 1102 26-27 0.22012782907725637 1102 28-29 0.24556157911079168 1102 30-31 0.2189764239086216 1102 32-33 0.17321340944637598 1102 34-35 0.25962627771503577 1102 36-37 0.17683643408826555 1102 38-39 0.15239908050951811 1102 40-41 0.029495732659441387 1102 42-43 0.04477141316118605 1102 44-45 0.06592376012692824 1102 46-47 0.10860619307214137 1102 48-49 0.28882002056243294 1102 50-51 0.3017172714472167 1102 52-53 0.3652718068325882 1102 54-55 0.4218573699127717 1102 56-57 0.4833297804931078 1102 58-59 0.5428815374136136 1102 60-61 0.4771352858326239 1102 62-63 0.41107639738051915 1102 64-65 0.5675819526008041 1102 66-67 0.33779214959238146 1102 68-69 0.218792929559676 1102 70-71 0.3688209046234263 1102 72-73 0.3694844171206526 1102 74-75 0.2962840390721908 1102 76-77 0.45395559124717977 1102 78-79 0.38686900549152625 1102 80-81 0.40164832156092345 1102 82-83 0.2951281366641467 1102 84-85 0.1474628391977575 1102 86-87 0.21332771850048005 1102 88-89 0.2321405401120451 1102 90-91 0.31257505516120787 1102 92-93 0.4872928750959957 1102 94-95 0.40225805903794765 1102 96-97 0.39801684677598104 1102 98-99 0.5190122435774533 1102 100-101 0.4370517549700388 1103 1 0.33567417528245613 1103 2 0.34009192057492754 1103 3 0.39364393783947094 1103 4 0.1488943640939553 1103 5 0.16832816029265274 1103 6 0.056239837958720784 1103 7 0.08554701833566725 1103 8 0.1300347349067934 1103 9 0.17829657762761286 1103 10-11 0.3217477952983856 1103 12-13 0.2649104495076884 1103 14-15 0.27506371597775825 1103 16-17 0.41958330868924065 1103 18-19 0.3210569124674194 1103 20-21 0.3253492552550554 1103 22-23 0.43269383683341545 1103 24-25 0.5303363736633386 1103 26-27 0.5104949936730989 1103 28-29 0.5073890257113689 1103 30-31 0.48861903175449584 1103 32-33 0.4346841619243875 1103 34-35 0.4346388422829506 1103 36-37 0.3548721733036757 1103 38-39 0.5853594323174178 1103 40-41 0.5813657591846422 1103 42-43 0.6592520776872206 1103 44-45 0.6160843073836588 1103 46-47 0.6603784951801899 1103 48-49 0.8995292206182697 1103 50-51 0.9351376260472435 1103 52-53 0.9387947720706151 1103 54-55 0.9140596594562567 1103 56-57 0.9833109336412278 1103 58-59 0.9148004261473801 1103 60-61 0.9643536301017903 1103 62-63 1.0862306623532945 1103 64-65 0.9996753492223363 1103 66-67 0.913772185890025 1103 68-69 0.9554912403233189 1103 70-71 0.7405478346806689 1103 72-73 0.7875934496489236 1103 74-75 0.786972298181503 1103 76-77 0.7927905765885157 1103 78-79 0.815902231793352 1103 80-81 0.8899925907218851 1103 82-83 0.9039526515322187 1103 84-85 0.7939580227800569 1103 86-87 0.7957575663296019 1103 88-89 0.7806963128329727 1103 90-91 0.867714661471414 1103 92-93 0.9101240910669581 1103 94-95 0.8671393936742717 1103 96-97 0.8622196947447094 1103 98-99 0.9342823817876997 1103 100-101 0.9928907888943748 1104 1 0.3302022552589321 1104 2 0.297201594200331 1104 3 0.31338225010056675 1104 4 0.2981181218122728 1104 5 0.294135275846898 1104 6 0.26945065830567927 1104 7 0.28923710948533454 1104 8 0.1782293835395521 1104 9 0.27398020009159296 1104 10-11 0.42198302023222567 1104 12-13 0.3936902201633714 1104 14-15 0.4361016459983347 1104 16-17 0.5076735762576021 1104 18-19 0.3797455334612394 1104 20-21 0.4177323778660522 1104 22-23 0.3308576121936042 1104 24-25 0.46033196319582714 1104 26-27 0.29298543766015683 1104 28-29 0.32135844646997924 1104 30-31 0.5391629894140024 1104 32-33 0.5995459672757519 1104 34-35 0.5620557784781894 1104 36-37 0.5534490624539288 1104 38-39 0.6820324696593758 1104 40-41 0.5906674351622954 1104 42-43 0.8077598695131485 1104 44-45 0.8289040662781062 1104 46-47 0.808330568099926 1104 48-49 0.8042572417885125 1104 50-51 0.8236027833539197 1104 52-53 0.7185904204093418 1104 54-55 0.813011438344823 1104 56-57 0.7284285461081481 1104 58-59 0.8256986913634918 1104 60-61 0.798721463092086 1104 62-63 0.9330374838763831 1104 64-65 0.8619820237298299 1104 66-67 0.63343846051513 1104 68-69 0.5931301700498679 1104 70-71 0.5600979669946966 1104 72-73 0.6637607533831229 1104 74-75 0.6735938800691841 1104 76-77 0.5462380920251633 1104 78-79 0.5793085828665667 1104 80-81 0.6534680391194136 1104 82-83 0.74334694732757 1104 84-85 0.7240035976109844 1104 86-87 0.706955743336362 1104 88-89 0.6157786415598174 1104 90-91 0.6899419475637387 1104 92-93 0.6693772519020058 1104 94-95 0.5875995524511062 1104 96-97 0.7761876453474734 1104 98-99 0.8881459513260737 1104 100-101 0.9197725883651167 1105 1 0.19408922317028754 1105 2 0.29590931631756234 1105 3 0.3562106655543644 1105 4 0.15642040966995552 1105 5 0.2031133405850838 1105 6 0.1940460048305539 1105 7 0.2231292082657177 1105 8 0.30786732265111283 1105 9 0.20168625547311336 1105 10-11 0.33260136962210396 1105 12-13 0.3078566770274236 1105 14-15 0.4187416215231323 1105 16-17 0.28531318529175564 1105 18-19 0.19950288478555223 1105 20-21 0.3692623966367421 1105 22-23 0.3556510230104948 1105 24-25 0.4067754105024406 1105 26-27 0.23756137611522377 1105 28-29 0.26133174045602203 1105 30-31 0.12489518991944237 1105 32-33 0.22684091575991516 1105 34-35 0.1755931461830329 1105 36-37 0.1558680600542104 1105 38-39 0.10282858294641528 1105 40-41 0.04525908491196873 1105 42-43 0.3254806482579653 1105 44-45 0.3535539083684398 1105 46-47 0.28987664872148855 1105 48-49 0.3835388882557069 1105 50-51 0.23954480772076892 1105 52-53 0.30930832777962536 1105 54-55 0.21859309086707412 1105 56-57 0.4629733169240211 1105 58-59 0.10004992325008999 1105 60-61 0.11011262971148028 1105 62-63 0.23881210169037814 1105 64-65 0.33786056554323096 1105 66-67 0.3552558079514512 1105 68-69 0.30966814007412324 1105 70-71 0.2239981604380148 1105 72-73 0.2979595888989337 1105 74-75 0.2520697251488073 1105 76-77 0.3529738713914128 1105 78-79 0.3495412566229774 1105 80-81 0.30985358090412873 1105 82-83 0.3287015031249787 1105 84-85 0.14381225762558358 1105 86-87 0.23293578712403118 1105 88-89 0.18795769932830098 1105 90-91 0.2947004302287297 1105 92-93 0.25635281175630453 1105 94-95 0.3571833784222278 1105 96-97 0.27470160545417244 1105 98-99 0.4395959795299156 1105 100-101 0.2451358124629941 1106 1 0.16262953439152739 1106 2 0.20179041366958472 1106 3 0.061514765536330174 1106 4 0.16334901536648516 1106 5 0.12020125306642626 1106 6 0.07669820144064943 1106 7 0.18122921546027726 1106 8 0.23737012358770215 1106 9 0.18781745094060653 1106 10-11 0.24081848457387878 1106 12-13 0.29782673603991583 1106 14-15 0.3680161808218685 1106 16-17 0.37887374188520084 1106 18-19 0.2860079930978614 1106 20-21 0.44015137561871853 1106 22-23 0.2559339979055508 1106 24-25 0.4151872560995571 1106 26-27 0.2832920490731041 1106 28-29 0.17161874226216156 1106 30-31 0.3767950156472821 1106 32-33 0.2882395462009768 1106 34-35 0.3242183873800073 1106 36-37 0.1894765713280222 1106 38-39 0.3061775340571202 1106 40-41 0.2728810012647429 1106 42-43 0.3011048791469335 1106 44-45 0.29231672944299447 1106 46-47 0.20313166471490973 1106 48-49 0.14717165305921043 1106 50-51 0.27732122311523 1106 52-53 0.3374953414857842 1106 54-55 0.43083994699519224 1106 56-57 0.29235912944454867 1106 58-59 0.3731892328922797 1106 60-61 0.46695201938042175 1106 62-63 0.42430062806254654 1106 64-65 0.3701832612618432 1106 66-67 0.5285768230058281 1106 68-69 0.5048626814785067 1106 70-71 0.346282254412003 1106 72-73 0.5259570888725094 1106 74-75 0.6146848850880957 1106 76-77 0.7163292873822442 1106 78-79 0.4912888678395362 1106 80-81 0.5464153421611506 1106 82-83 0.5652610480465157 1106 84-85 0.7810249054679481 1106 86-87 0.6673527152898693 1106 88-89 0.5068062005229059 1106 90-91 0.6610016970519972 1106 92-93 0.7132178086244494 1106 94-95 0.6817080852777586 1106 96-97 0.4358169200254842 1106 98-99 0.1669775087263865 1106 100-101 0.33976293788609624 1107 1 0.2462148887683 1107 2 0.14939123268499088 1107 3 0.15965417620132882 1107 4 0.18234661173433153 1107 5 0.19559899695878613 1107 6 0.18812753592704468 1107 7 0.1934737500585939 1107 8 0.2806202412257832 1107 9 0.207751064909651 1107 10-11 0.36378906869464345 1107 12-13 0.31641100941809697 1107 14-15 0.22946141234633188 1107 16-17 0.3691345804857491 1107 18-19 0.2553394272650493 1107 20-21 0.30887109176121186 1107 22-23 0.2553070187243094 1107 24-25 0.3961362456838131 1107 26-27 0.1835553040234359 1107 28-29 0.2747078546987396 1107 30-31 0.34321029715203366 1107 32-33 0.3572797466308373 1107 34-35 0.401947272934045 1107 36-37 0.3471790042107301 1107 38-39 0.18431719907473365 1107 40-41 0.32594022726974714 1107 42-43 0.47017433012682375 1107 44-45 0.4337774764766067 1107 46-47 0.2658232240235776 1107 48-49 0.3596044085881971 1107 50-51 0.39230767883879736 1107 52-53 0.5551817501541265 1107 54-55 0.6054746330604814 1107 56-57 0.6076902329533453 1107 58-59 0.6362953869536554 1107 60-61 0.4975379206153079 1107 62-63 0.5559459079140083 1107 64-65 0.5595945621482628 1107 66-67 0.4196808405052863 1107 68-69 0.3886235391555104 1107 70-71 0.4013445072130608 1107 72-73 0.4925758524644763 1107 74-75 0.5659356640429678 1107 76-77 0.44630601411851956 1107 78-79 0.41810907869785296 1107 80-81 0.5979001255163148 1107 82-83 0.5977024515642135 1107 84-85 0.4490108143334055 1107 86-87 0.30094633612643307 1107 88-89 0.3740277797982543 1107 90-91 0.44920482304555875 1107 92-93 0.46749411186362977 1107 94-95 0.4758795952420236 1107 96-97 0.3166997779314009 1107 98-99 0.5501902365309412 1107 100-101 0.6139474198334227 1108 1 0.05410670625710168 1108 2 0.11225457544816209 1108 3 0.12996715447631146 1108 4 0.13596468582515797 1108 5 0.11528369859318133 1108 6 0.028919018269988328 1108 7 0.061926951491201976 1108 8 0.11940747721799028 1108 9 0.22374136342147466 1108 10-11 0.1835856805824747 1108 12-13 0.12972696481777035 1108 14-15 0.2898484970452344 1108 16-17 0.3016634207718809 1108 18-19 0.25342583991753287 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-2.1515708901413646 2316 78-79 -2.2529063135941385 2316 80-81 -2.407476984116112 2316 82-83 -2.4592721522189045 2316 84-85 -2.482166259396859 2316 86-87 -2.846759424345283 2316 88-89 -2.7984425628848513 2316 90-91 -2.56613787527586 2316 92-93 -2.7396720208344156 2316 94-95 -3.3344946087492033 2316 96-97 -3.2985513394473784 2316 98-99 -3.1600835631045605 2316 100-101 -3.153067011010794 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2683.0 3 744.0 4 650.0 5 644.0 6 689.0 7 785.0 8 921.0 9 1128.0 10 1421.0 11 1951.0 12 2804.0 13 3968.0 14 4521.0 15 6066.0 16 8593.0 17 12745.0 18 10856.0 19 5695.0 20 5091.0 21 5079.0 22 5285.0 23 5526.0 24 6171.0 25 6995.0 26 8069.0 27 9936.0 28 11888.0 29 14826.0 30 19325.0 31 25316.0 32 35047.0 33 50279.0 34 77725.0 35 126313.0 36 211876.0 37 330368.0 38 286907.0 39 45557.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.03767365842383 18.711942116197434 14.824120417452288 23.426263807926453 2 14.88707578646682 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22.054914317326872 25.865413094554878 30.52420763562207 21.555464952496177 22-23 21.757849467606466 25.732828060722806 30.568365223035908 21.940957248634817 24-25 21.57531945372582 25.97228016673251 30.532724392936387 21.91967598660528 26-27 21.394252423743456 26.232029594406008 30.379602897416397 21.994115084434142 28-29 21.251694612387876 26.195532425798866 30.551683093453995 22.001089868359262 30-31 21.348739795181874 26.06474745509474 30.515493528687937 22.071019221035446 32-33 21.216832144457793 26.164183699914574 30.41497060237263 22.204013553255002 34-35 21.646956235385154 26.249650203528013 30.135215256811527 21.968178304275305 36-37 21.635283789660523 26.042508828626087 30.216568062191328 22.105639319522062 38-39 21.45080620033698 26.207749978218786 30.259388442517526 22.08205537892671 40-41 21.724020178788646 26.11665377650866 30.240864446606135 21.918461598096563 42-43 21.40349219205395 26.271216161291 29.939350490227746 22.385941156427304 44-45 21.493758716644184 26.46345488091467 29.737221556514864 22.305564845926277 46-47 21.71156408241195 26.53326339050212 29.357014469823127 22.3981580572628 48-49 21.827322720673276 26.743593255277137 29.01499241574911 22.41409160830048 50-51 21.79905707985657 26.844650750013198 28.94261299850599 22.41367917162424 52-53 21.934341612772112 26.945713643372677 28.709532017222138 22.41041272663307 54-55 21.73941453214846 26.842138682402002 28.81824773110534 22.6001990543442 56-57 21.761183041952815 27.17763757119603 28.624282501699255 22.436896885151906 58-59 21.889503847511264 26.89765516671072 28.697368984525784 22.51547200125223 60-61 21.939036019941057 26.749743315970008 29.054010897949222 22.257209766139713 62-63 21.714430224594864 26.82475250631771 29.134053617016566 22.32676365207086 64-65 21.671749590123536 26.873793490190838 29.011353236538127 22.4431036831475 66-67 21.575231707100958 27.16226733299072 28.637092629676232 22.62540833023209 68-69 21.498494490258953 27.056788455112546 28.811339599059956 22.63337745556854 70-71 21.662606147892475 26.90121069791328 28.744190270777963 22.691992883416283 72-73 21.757667273378402 26.903518066595534 28.684444674166265 22.6543699858598 74-75 21.665746728373296 27.20789805483817 28.45762345787267 22.668731758915865 76-77 21.72756582085739 27.035362419011584 28.534875786992526 22.702195973138505 78-79 21.73424005601041 27.051388665277237 28.48155954724918 22.73281173146317 80-81 21.647487932980034 27.21504404388437 28.473736138435452 22.66373188470014 82-83 21.678290175510245 27.20436488081765 28.443877598119165 22.673467345552943 84-85 21.687159352632467 27.171780423165163 28.238296056114837 22.90276416808753 86-87 21.640304822666756 27.053233202631755 28.53152714088449 22.774934833817003 88-89 21.718362901290405 26.994775609666043 28.372057819047797 22.914803669995752 90-91 21.612526034061283 27.103963823131206 28.438591367601866 22.84491877520564 92-93 21.73763419261408 27.054936907224413 28.362394091500214 22.84503480866129 94-95 21.649572842066945 26.981192054740603 28.30783874220366 23.06139636098879 96-97 21.683936101858894 26.997850860025256 28.286485528385636 23.031727509730214 98-99 21.584998646427213 27.015835508959984 28.103088694287926 23.296077150324876 100-101 21.559729937359652 26.9491638104243 28.284629476421223 23.206476775794822 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 553.0 1 402.5 2 307.5 3 395.5 4 344.5 5 311.5 6 619.5 7 1389.0 8 2401.0 9 2824.5 10 2325.5 11 1861.5 12 1850.0 13 1872.0 14 1972.0 15 2142.0 16 2255.5 17 2363.0 18 2440.5 19 2587.5 20 2869.0 21 3294.5 22 3943.5 23 4713.5 24 5560.5 25 6865.0 26 8616.0 27 10557.0 28 12435.0 29 14330.5 30 16571.5 31 19181.0 32 21799.5 33 24512.0 34 27604.0 35 29968.0 36 32403.0 37 35159.5 38 36829.5 39 38006.0 40 39565.5 41 41757.0 42 43821.5 43 45290.0 44 46900.0 45 48598.0 46 48836.0 47 49790.5 48 53266.5 49 53465.5 50 50314.0 51 48303.5 52 46512.0 53 45134.5 54 43506.0 55 40542.5 56 37231.0 57 33287.5 58 29595.5 59 26060.5 60 22204.5 61 18133.0 62 14280.0 63 11179.0 64 8541.5 65 6550.0 66 4757.5 67 3506.0 68 2475.0 69 1476.5 70 999.5 71 726.0 72 520.5 73 359.0 74 259.0 75 192.5 76 119.5 77 63.5 78 42.5 79 31.0 80 18.5 81 16.0 82 14.0 83 10.5 84 8.0 85 7.5 86 5.0 87 2.0 88 3.0 89 4.0 90 4.0 91 3.5 92 3.5 93 3.5 94 2.5 95 1.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.032411392862996526 2 0.027169459165336498 3 0.020229434269842934 4 0.0362505555711419 5 0.055520199163948486 6 0.0551510489035499 7 0.054412748382752715 8 0.055520199163948486 9 0.05197635666412199 10-11 0.056000094502466666 12-13 0.05548328413790863 14-15 0.049724540075690576 16-17 0.042784515180197005 18-19 0.03735800635233768 20-21 0.042156959737519394 22-23 0.025212962785223947 24-25 0.014544520259704593 26-27 0.011222167916117248 28-29 0.012551108853552186 30-31 0.010225462213041044 32-33 0.007456835260051592 34-35 0.00760449536421103 36-37 0.004503633176862843 38-39 0.006201724374696373 40-41 0.005131188619540453 42-43 0.002990117109228609 44-45 9.597906770363436E-4 46-47 0.0016611761717936716 48-49 7.752155468370467E-4 50-51 0.0038760777341852342 52-53 0.0031377772133880464 54-55 0.004282143020623687 56-57 0.01406462492118642 58-59 0.005906404166377499 60-61 0.012588023879592044 62-63 0.00993014200472217 64-65 0.006681619713214545 66-67 0.012255788645233311 68-69 0.0198971990354842 70-71 0.011849723358794858 72-73 0.012329618697313029 74-75 0.01509824565030248 76-77 0.018900493332407998 78-79 0.03159926229011962 80-81 0.02643115864453931 82-83 0.028166164868412696 84-85 0.017645382447052778 86-87 0.01712857208249475 88-89 0.017497722342893342 90-91 0.016722506796056295 92-93 0.025471367967502965 94-95 0.016279526483577982 96-97 0.031894582498438497 98-99 0.04706665820082069 100-101 0.05182869655996255 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1354462.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.90409218782688 #Duplication Level Percentage of deduplicated Percentage of total 1 79.41698868804299 48.368196003361746 2 12.198346526200416 14.858584427415359 3 3.6635203818448696 6.6937014920358795 4 1.569915594508002 3.8245713638008967 5 0.8319550683570421 2.533473408967355 6 0.5192724601620488 1.8975490670585446 7 0.3338995737275293 1.423509529384428 8 0.2405225967293089 1.171904832356587 9 0.17643943622261477 0.9671295320343298 >10 0.9496447184774387 10.49100802798692 >50 0.06782173848677314 2.786946265494994 >100 0.03021700032722292 3.1382395638664495 >500 8.494598662861613E-4 0.3266732463816992 >1k 3.6405422840835483E-4 0.46916782082149927 >5k 2.427028189389032E-4 1.0493454190333407 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8309 0.613453902730383 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5889 0.43478517669746364 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3906 0.2883801834233814 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1379 0.10181164181793213 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 7.383005207971874E-5 7 0.0 0.0 0.0 0.0 7.383005207971874E-5 8 0.0 0.0 0.0 0.0 7.383005207971874E-5 9 0.0 0.0 0.0 0.0 7.383005207971874E-5 10-11 0.0 0.0 0.0 0.0 7.383005207971874E-5 12-13 0.0 0.0 0.0 0.0 7.383005207971874E-5 14-15 0.0 0.0 0.0 0.0 7.383005207971874E-5 16-17 0.0 0.0 0.0 0.0 7.383005207971874E-5 18-19 0.0 0.0 0.0 3.691502603985937E-5 7.383005207971874E-5 20-21 0.0 0.0 0.0 1.1074507811957812E-4 7.383005207971874E-5 22-23 0.0 0.0 0.0 4.7989533851817176E-4 7.383005207971874E-5 24-25 0.0 0.0 0.0 9.597906770363435E-4 7.383005207971874E-5 26-27 0.0 0.0 0.0 0.001698091197833531 1.1074507811957812E-4 28-29 0.0 0.0 0.0 0.0037653326560656555 1.4766010415943748E-4 30-31 0.0 0.0 0.0 0.007567580338171171 1.4766010415943748E-4 32-33 0.0 0.0 0.0 0.013252494348309513 2.214901562391562E-4 34-35 0.0 0.0 0.0 0.01875283322824856 2.214901562391562E-4 36-37 0.0 0.0 0.0 0.027132544139296638 2.214901562391562E-4 38-39 0.0 0.0 0.0 0.038317797029374025 2.214901562391562E-4 40-41 0.0 0.0 0.0 0.05585243439830723 2.214901562391562E-4 42-43 0.0 0.0 0.0 0.07364547694951944 2.584051822790156E-4 44-45 0.0 0.0 0.0 0.09826779931810564 2.9532020831887497E-4 46-47 0.0 0.0 0.0 0.13222962327477625 3.322352343587343E-4 48-49 0.0 0.0 0.0 0.16161398400250432 4.429803124783124E-4 50-51 0.0 0.0 0.0 0.1958711281674938 4.429803124783124E-4 52-53 0.0 0.0 0.0 0.22591995936393933 5.537253905978905E-4 54-55 7.383005207971874E-5 0.0 0.0 0.2602878486070484 6.644704687174686E-4 56-57 7.383005207971874E-5 0.0 0.0 0.3112674995680942 6.644704687174686E-4 58-59 7.383005207971874E-5 0.0 0.0 0.36224715052913997 6.644704687174686E-4 60-61 7.383005207971874E-5 0.0 0.0 0.4098306190945187 6.644704687174686E-4 62-63 7.383005207971874E-5 0.0 0.0 0.4601088845608072 6.644704687174686E-4 64-65 7.383005207971874E-5 0.0 0.0 0.5149276982299984 6.644704687174686E-4 66-67 7.383005207971874E-5 0.0 0.0 0.5781631378362775 6.644704687174686E-4 68-69 7.383005207971874E-5 0.0 0.0 0.6555001173897828 6.644704687174686E-4 70-71 7.383005207971874E-5 0.0 0.0 0.7479722576196306 6.644704687174686E-4 72-73 7.383005207971874E-5 0.0 0.0 0.8409242931879963 6.644704687174686E-4 74-75 7.383005207971874E-5 0.0 0.0 0.9411855039122545 6.644704687174686E-4 76-77 7.383005207971874E-5 0.0 0.0 1.054145483594224 6.644704687174686E-4 78-79 7.383005207971874E-5 0.0 0.0 1.1829420094472933 6.644704687174686E-4 80-81 7.383005207971874E-5 0.0 0.0 1.3230714482945996 7.01385494757328E-4 82-83 7.383005207971874E-5 0.0 0.0 1.4753459307090195 7.383005207971874E-4 84-85 7.383005207971874E-5 0.0 0.0 1.640097691924912 7.383005207971874E-4 86-87 7.383005207971874E-5 7.383005207971874E-5 0.0 1.803889662463768 7.383005207971874E-4 88-89 7.383005207971874E-5 7.383005207971874E-5 0.0 1.978719225788542 7.752155468370467E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3520 0.0 46.704792 1 GTATCAA 6370 0.0 42.666264 1 TATCAAC 7960 0.0 34.085297 2 ATCAACG 7960 0.0 33.906216 3 TCAACGC 8065 0.0 33.464783 4 CAACGCA 8160 0.0 33.134636 5 AACGCAG 8400 0.0 32.242123 6 ACGCAGA 9535 0.0 28.203804 7 CGCAGAG 9635 0.0 27.565891 8 CCCGTAC 105 6.890463E-5 27.15226 3 GCAGAGT 10140 0.0 25.81818 9 TCGCGCA 130 1.0001626E-5 25.583895 9 GTACATG 5325 0.0 24.71657 1 GTATAGG 410 0.0 24.336798 1 GAGTACT 5585 0.0 23.775545 12-13 TACATGG 5730 0.0 22.887508 2 AGAGTAC 8700 0.0 22.172297 10-11 ACATGGG 5725 0.0 21.745523 3 GTACTTT 6250 0.0 21.32145 14-15 CAGAGTA 9620 0.0 21.311285 10-11 >>END_MODULE