FastQCFastQC Report
Thu 26 May 2016
SRR937062_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937062_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354462
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68310.5043330857565587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58650.4330132554475504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39330.2903735948295338No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15100.11148337864037529No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15060.11118805843205641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48300.040.0362241
GTATCAA75650.034.7313841
TATCAAC97600.026.8647542
ATCAACG98600.026.6876563
TCAACGC99250.026.5118984
CAACGCA101100.026.1207225
AACGCAG104150.025.4004526
ACGCAGA119250.021.9451587
GAGTACT67350.021.8261212-13
CGCAGAG119800.021.7254738
AGTACTT68900.021.33511412-13
GTACTTT69850.020.77410714-15
GCAGAGT124850.020.352179
AGAGTAC104500.020.2250310-11
TGCACCG3353.45608E-1119.8492815
ACCGTGC1801.2345743E-418.4701778
ATACGTC1559.630505E-418.3857385
TACTTTT79300.018.32845314-15
TCGCGCT1203.094332E-617.8118460-61
CAGAGTA122200.017.68421210-11
Produced by FastQC (version 0.11.5)