##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937062_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1354462 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.20478463035508 31.0 31.0 33.0 30.0 34.0 2 31.299810552086363 31.0 31.0 34.0 30.0 34.0 3 31.39110657958658 31.0 31.0 34.0 28.0 34.0 4 35.243982481605244 37.0 35.0 37.0 33.0 37.0 5 35.07883942111333 35.0 35.0 37.0 32.0 37.0 6 35.18013129936462 37.0 35.0 37.0 32.0 37.0 7 35.12507401462721 37.0 35.0 37.0 32.0 37.0 8 35.09529023331773 37.0 35.0 37.0 32.0 37.0 9 36.59249133604339 38.0 35.0 39.0 32.0 39.0 10-11 36.410901893150196 38.0 35.0 39.0 32.0 39.0 12-13 36.26761289722414 38.0 35.0 39.0 32.0 39.0 14-15 37.14451235988902 38.5 36.0 40.0 32.0 41.0 16-17 36.992701899351914 38.0 36.0 40.0 31.0 41.0 18-19 37.0096189483352 38.5 36.0 40.0 31.0 41.0 20-21 37.138339429234634 39.0 36.0 40.0 31.0 41.0 22-23 37.02668181167135 39.0 36.0 40.0 31.0 41.0 24-25 36.872148867963816 39.0 36.0 40.0 31.0 41.0 26-27 36.55818398744299 38.0 35.0 40.0 30.0 41.0 28-29 36.28629153125004 38.0 35.0 40.0 30.0 41.0 30-31 36.1559109816296 38.0 35.0 40.0 30.0 41.0 32-33 35.99535535142366 38.0 35.0 40.0 30.0 41.0 34-35 35.775038723862316 38.0 34.0 40.0 29.0 41.0 36-37 35.50107016660489 38.0 34.0 40.0 27.5 41.0 38-39 35.25549775482811 38.0 34.0 40.0 27.0 41.0 40-41 34.88729768719979 38.0 33.0 40.0 26.0 41.0 42-43 34.49807340479098 37.5 33.0 40.0 25.0 41.0 44-45 34.10620785226902 37.0 33.0 40.0 24.0 41.0 46-47 33.59458441801985 37.0 32.5 40.0 23.0 41.0 48-49 33.05907474702133 37.0 31.0 39.0 20.0 40.0 50-51 32.68055582216408 36.5 31.0 39.0 18.5 40.0 52-53 32.30467484506764 36.0 31.0 39.0 16.5 40.0 54-55 31.873588184829103 36.0 30.0 39.0 13.5 40.0 56-57 31.473819125232012 35.0 29.5 39.0 11.0 40.0 58-59 30.934323369721703 35.0 28.5 38.0 8.0 40.0 60-61 30.416823063326987 34.0 28.0 38.0 7.5 40.0 62-63 29.915862165199172 34.0 28.0 38.0 6.5 40.0 64-65 29.331821416916828 34.0 26.5 37.5 2.0 39.5 66-67 28.705939332369603 33.0 26.0 37.0 2.0 39.0 68-69 28.096729919333285 32.5 25.5 36.0 2.0 39.0 70-71 27.517638368592102 32.0 25.0 36.0 2.0 38.5 72-73 26.919355803263585 31.5 23.5 35.0 2.0 38.0 74-75 26.262069736913993 31.0 22.0 35.0 2.0 37.5 76-77 25.31356693654012 30.0 21.5 34.0 2.0 36.0 78-79 25.938007489320484 31.0 22.0 34.0 2.0 36.0 80-81 25.548089204422126 31.0 20.0 34.0 2.0 36.0 82-83 25.085978787149436 31.0 19.5 34.0 2.0 36.0 84-85 24.473924702206485 30.5 18.0 34.0 2.0 35.0 86-87 23.798506713366635 30.0 11.5 34.0 2.0 35.0 88-89 23.072175151462353 29.5 6.5 34.0 2.0 35.0 90-91 22.221620466281077 29.0 2.0 34.0 2.0 35.0 92-93 21.328036888447222 29.0 2.0 33.5 2.0 35.0 94-95 20.297745894679956 27.0 2.0 33.0 2.0 34.5 96-97 19.061773235424837 25.5 2.0 32.5 2.0 34.0 98-99 17.662192811610808 24.0 2.0 32.0 2.0 34.0 100-101 14.710281277732413 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38991158187255337 1101 2 0.3856178210500687 1101 3 0.4736790910505455 1101 4 0.3547891561560803 1101 5 0.5082317135492005 1101 6 0.5035672738883079 1101 7 0.3538350937958725 1101 8 0.35781673371521094 1101 9 0.5101004630642407 1101 10-11 0.4960084034753294 1101 12-13 0.4766242912023202 1101 14-15 0.5291705373285112 1101 16-17 0.4476772740931949 1101 18-19 0.46039521485572976 1101 20-21 0.4742713749586187 1101 22-23 0.5190216148554399 1101 24-25 0.573990309719072 1101 26-27 0.48587337899023453 1101 28-29 0.538432339618538 1101 30-31 0.46505163602741817 1101 32-33 0.49656601677627776 1101 34-35 0.6084452102511406 1101 36-37 0.7511369231682608 1101 38-39 0.7521295321254655 1101 40-41 0.8379800884373267 1101 42-43 0.6550578760766328 1101 44-45 0.6764903015426071 1101 46-47 0.8056597504081822 1101 48-49 0.7769307658751785 1101 50-51 0.7030080322535568 1101 52-53 0.793141204269908 1101 54-55 0.8152541258156276 1101 56-57 0.8221609881749465 1101 58-59 0.8628880295431749 1101 60-61 0.9553625839915476 1101 62-63 0.8701122672562178 1101 64-65 0.8901729904204565 1101 66-67 0.9970961467332131 1101 68-69 0.8965490237271503 1101 70-71 0.9803286049064361 1101 72-73 1.0488909874653913 1101 74-75 0.9332763230919205 1101 76-77 0.8231828151807186 1101 78-79 1.1579767610284861 1101 80-81 1.368865618889192 1101 82-83 1.3431043601846255 1101 84-85 1.012004070544851 1101 86-87 1.3788841211209437 1101 88-89 1.4756503343964162 1101 90-91 1.3638528574267816 1101 92-93 1.4425989083458006 1101 94-95 1.20698565542747 1101 96-97 1.0803180969711335 1101 98-99 1.3220319534956815 1101 100-101 1.4966248516909584 1102 1 0.2621041011579486 1102 2 0.28354614492855745 1102 3 0.2719755592438169 1102 4 0.2573481878618509 1102 5 0.3429968728801782 1102 6 0.33595954811295314 1102 7 0.32515413468450305 1102 8 0.30848373598050216 1102 9 0.36604871818188656 1102 10-11 0.3816309988512572 1102 12-13 0.3163369948088146 1102 14-15 0.36493571602094477 1102 16-17 0.3664152941903893 1102 18-19 0.302848532994922 1102 20-21 0.4335401203084075 1102 22-23 0.37692523904399167 1102 24-25 0.40993153939299987 1102 26-27 0.33196388551366596 1102 28-29 0.4477427121053523 1102 30-31 0.614469547589799 1102 32-33 0.43697946690531353 1102 34-35 0.4168406155003055 1102 36-37 0.34275686879021805 1102 38-39 0.27342146697138503 1102 40-41 0.5950784142242966 1102 42-43 0.738281448376469 1102 44-45 0.6660577942060044 1102 46-47 0.6477974767643815 1102 48-49 0.8653802725728568 1102 50-51 0.8745726870802315 1102 52-53 0.6759162821997791 1102 54-55 0.5466993480990112 1102 56-57 0.6993118388344861 1102 58-59 0.6615054830687583 1102 60-61 0.7118991508729415 1102 62-63 0.7046692909020891 1102 64-65 0.6245308676300816 1102 66-67 0.5474700994857216 1102 68-69 0.5391568186505786 1102 70-71 0.712179726349806 1102 72-73 0.6842964579073616 1102 74-75 0.6898063900549083 1102 76-77 0.7124608540308692 1102 78-79 0.6954810136346765 1102 80-81 0.7508818659192826 1102 82-83 0.7011982798566976 1102 84-85 0.5358976323065221 1102 86-87 0.6352276265960874 1102 88-89 0.5283583110409644 1102 90-91 0.4476267100528837 1102 92-93 0.5872052207346456 1102 94-95 1.0604362823238596 1102 96-97 0.8734328855301712 1102 98-99 0.9713440685138117 1102 100-101 1.0566072051039672 1103 1 0.2887870552274734 1103 2 0.2652144403355088 1103 3 0.2976854573312089 1103 4 0.3753127278590611 1103 5 0.35431754857471986 1103 6 0.3054025189352316 1103 7 0.252046498219336 1103 8 0.27944562345781776 1103 9 0.371046624087235 1103 10-11 0.27059651609264534 1103 12-13 0.39567456286688696 1103 14-15 0.6174960957501128 1103 16-17 0.42750631750463697 1103 18-19 0.44123957026980776 1103 20-21 0.4314641744756571 1103 22-23 0.443777116748862 1103 24-25 0.473439845968457 1103 26-27 0.5422745095538701 1103 28-29 0.5362935986054254 1103 30-31 0.5277509939111766 1103 32-33 0.5869285106020783 1103 34-35 0.42928023577113805 1103 36-37 0.25093221431583146 1103 38-39 0.39946502414017004 1103 40-41 0.43481176617181205 1103 42-43 0.3207161824264517 1103 44-45 0.4915624710173958 1103 46-47 0.6295390654841952 1103 48-49 0.41307258026517246 1103 50-51 0.5365676612530592 1103 52-53 0.6698008277812448 1103 54-55 0.5738974494531845 1103 56-57 0.37778663989549344 1103 58-59 0.3770680964988884 1103 60-61 0.3705505539163596 1103 62-63 0.4412768575641053 1103 64-65 0.3834307699056687 1103 66-67 0.20605449150023958 1103 68-69 0.29647218930533015 1103 70-71 0.4192633505296186 1103 72-73 0.6475960530490603 1103 74-75 0.5744894837307442 1103 76-77 0.7438638974484313 1103 78-79 0.8469084881565259 1103 80-81 1.1932007267317921 1103 82-83 1.1270059023612333 1103 84-85 0.995149342483824 1103 86-87 0.8348527836531865 1103 88-89 0.7482640767815738 1103 90-91 0.6103113393981339 1103 92-93 0.7004217501217731 1103 94-95 0.8904558272072869 1103 96-97 1.2468553342248505 1103 98-99 1.6817314760246305 1103 100-101 1.6027205654278536 1104 1 0.28719634661235816 1104 2 0.208457604110869 1104 3 0.2144099501274468 1104 4 0.2548305167446827 1104 5 0.10264745154443489 1104 6 0.28663156921456334 1104 7 0.32705090868685005 1104 8 0.31896341234343595 1104 9 0.30935888518691 1104 10-11 0.33160210268481904 1104 12-13 0.3365257830962278 1104 14-15 0.4450232936330849 1104 16-17 0.4900864409977288 1104 18-19 0.35174677403340837 1104 20-21 0.42468381575763203 1104 22-23 0.4048856142496007 1104 24-25 0.4010581935133004 1104 26-27 0.4376038244612488 1104 28-29 0.4823447600285391 1104 30-31 0.45749371663978167 1104 32-33 0.40445423832922245 1104 34-35 0.5537921440334159 1104 36-37 0.5076302443070091 1104 38-39 0.5800001608646639 1104 40-41 0.6813216162159179 1104 42-43 0.6102751356754936 1104 44-45 0.7481399482299835 1104 46-47 0.7560151019440582 1104 48-49 0.908826770209302 1104 50-51 1.00259530018279 1104 52-53 1.051198945981767 1104 54-55 0.9535240298707208 1104 56-57 0.9322838465994074 1104 58-59 0.7860286963224681 1104 60-61 0.9639818976387922 1104 62-63 1.012111906079248 1104 64-65 0.8577177443249546 1104 66-67 0.743578749071542 1104 68-69 0.8401652207666679 1104 70-71 0.8604629976922169 1104 72-73 1.0127665911260983 1104 74-75 1.225549466088875 1104 76-77 1.1555428154137353 1104 78-79 1.1563689409645228 1104 80-81 1.2416247196750447 1104 82-83 1.2726777635785211 1104 84-85 1.0256580164032592 1104 86-87 1.1170771294338415 1104 88-89 1.0661426419094795 1104 90-91 1.0318138386630515 1104 92-93 1.1759171926386784 1104 94-95 1.2529492115060243 1104 96-97 1.48720376115811 1104 98-99 1.3989073066626787 1104 100-101 1.4587129206175025 1105 1 0.20246976456785148 1105 2 0.20705219215944837 1105 3 0.19070374177591987 1105 4 0.16061851943440786 1105 5 0.30153555079647987 1105 6 0.17401632384110854 1105 7 0.22815452355935406 1105 8 0.2140839877656191 1105 9 0.13948569095005325 1105 10-11 0.22247329875093413 1105 12-13 0.3207727404271594 1105 14-15 0.26253437599149976 1105 16-17 0.3005613810048047 1105 18-19 0.3689077781540391 1105 20-21 0.22533122944908257 1105 22-23 0.03513208717549787 1105 24-25 0.24555111387009987 1105 26-27 0.2855898786954896 1105 28-29 0.3112505145690392 1105 30-31 0.2201935735490892 1105 32-33 0.27916542776664244 1105 34-35 0.29562529837060225 1105 36-37 0.24059384700565545 1105 38-39 0.20260100156233563 1105 40-41 0.3785686340974692 1105 42-43 0.5217904310828914 1105 44-45 0.7667205458965469 1105 46-47 0.7555445598247275 1105 48-49 0.7906995055361321 1105 50-51 0.6573701952549271 1105 52-53 0.47178074191340613 1105 54-55 0.20907684117264935 1105 56-57 0.10005418125316723 1105 58-59 0.34090828024404374 1105 60-61 0.29698103399562115 1105 62-63 0.422993013327158 1105 64-65 0.26713990011853284 1105 66-67 0.1632538790163771 1105 68-69 0.4485047950638794 1105 70-71 0.24684133713903833 1105 72-73 0.28887761548338275 1105 74-75 0.2537531520287004 1105 76-77 0.4777438626209154 1105 78-79 0.6023450330269746 1105 80-81 0.6925981506967211 1105 82-83 0.7344535860313322 1105 84-85 0.592549888915503 1105 86-87 0.5275079312197626 1105 88-89 0.10729476102212487 1105 90-91 0.14854684544791397 1105 92-93 0.4933202399287211 1105 94-95 0.2915676055121459 1105 96-97 0.3521988055672125 1105 98-99 0.04152236082362393 1105 100-101 0.009079889293706955 1106 1 0.1569479386074697 1106 2 0.17336525670697966 1106 3 0.2488654994868078 1106 4 0.23048559727108398 1106 5 0.19213143475460726 1106 6 0.2933762188754585 1106 7 0.1771866498536525 1106 8 0.1346065382972128 1106 9 0.299017178087297 1106 10-11 0.2689467850705256 1106 12-13 0.027129088301812487 1106 14-15 0.3265088997265764 1106 16-17 0.2630096679309162 1106 18-19 0.32721953063096265 1106 20-21 0.08937558480063501 1106 22-23 0.29916328090401834 1106 24-25 0.1353491170541261 1106 26-27 0.17678877639251311 1106 28-29 0.11087094154436272 1106 30-31 0.05653902046290682 1106 32-33 0.149791788042549 1106 34-35 0.20452027100566994 1106 36-37 0.0699324345316441 1106 38-39 0.05979943757962758 1106 40-41 0.12785011518240452 1106 42-43 0.060149913196895 1106 44-45 0.1510235714672632 1106 46-47 0.16447362992877856 1106 48-49 0.2201138235407214 1106 50-51 -0.04670946461600067 1106 52-53 -0.03730128943649191 1106 54-55 0.17762601773582531 1106 56-57 0.16501616768987404 1106 58-59 0.23948433584201467 1106 60-61 0.1511187107100156 1106 62-63 0.1210459455560482 1106 64-65 0.367093902632881 1106 66-67 0.21263076481952226 1106 68-69 0.37615098881456177 1106 70-71 0.34974562315679236 1106 72-73 0.54566019876901 1106 74-75 0.34848938935254026 1106 76-77 0.3121761005503281 1106 78-79 0.39926715679464664 1106 80-81 0.8155346997396258 1106 82-83 0.8277848371458916 1106 84-85 0.5867408736129001 1106 86-87 0.5377774069268533 1106 88-89 0.35969752441740255 1106 90-91 0.1631131137083841 1106 92-93 0.5691690367483808 1106 94-95 0.8044939560020801 1106 96-97 1.0479273133358085 1106 98-99 0.9699076172144245 1106 100-101 0.8217412342547323 1107 1 0.1659571080190254 1107 2 0.019888892423175264 1107 3 -0.0821769646812669 1107 4 0.12657492590737007 1107 5 0.10704031293242622 1107 6 0.1663143730385741 1107 7 0.10781197574293344 1107 8 0.11198441725080954 1107 9 0.07429410449037022 1107 10-11 0.05242022429932547 1107 12-13 0.1914968313039367 1107 14-15 0.09938219397439241 1107 16-17 0.10552343476587822 1107 18-19 0.1787831632949306 1107 20-21 0.027223253023088034 1107 22-23 0.0506048243156485 1107 24-25 0.21135857897117916 1107 26-27 0.1149772771110591 1107 28-29 0.06601204365421154 1107 30-31 0.18422275842885227 1107 32-33 0.10296674448466092 1107 34-35 -0.03797060409447539 1107 36-37 -0.036289030285033164 1107 38-39 0.007327766101454358 1107 40-41 0.0997021837049985 1107 42-43 -0.15929661552587504 1107 44-45 -0.15299519975528142 1107 46-47 0.24995899829494306 1107 48-49 0.27884765625301355 1107 50-51 0.21734033762484728 1107 52-53 0.14729002950896586 1107 54-55 -0.11428684106032705 1107 56-57 -0.21718296496772993 1107 58-59 -0.06743278336033498 1107 60-61 -0.13369516676833015 1107 62-63 0.16888204073479685 1107 64-65 0.2114262906223523 1107 66-67 -0.08655149154247255 1107 68-69 0.21156577433172785 1107 70-71 -0.14973041046862434 1107 72-73 0.0655683774771525 1107 74-75 0.10994861027049652 1107 76-77 0.19534686017439284 1107 78-79 0.32715564861085156 1107 80-81 0.4937430399616751 1107 82-83 0.6057261071284721 1107 84-85 0.39258335304213077 1107 86-87 0.5041608943554756 1107 88-89 0.09089005662479721 1107 90-91 0.10074966926535112 1107 92-93 0.44843166853482685 1107 94-95 0.288342565329188 1107 96-97 0.032069699926342565 1107 98-99 0.02497495706766273 1107 100-101 0.0715943456230228 1108 1 0.07606938870709712 1108 2 0.0719856499503102 1108 3 0.06664261610370303 1108 4 0.07854827021077426 1108 5 0.09818068621355991 1108 6 0.1957424804490273 1108 7 0.2490314075741864 1108 8 0.15993581622645792 1108 9 0.02768211917518215 1108 10-11 0.14674789518179665 1108 12-13 0.13424635315080735 1108 14-15 0.15315897006225043 1108 16-17 0.11776103544124084 1108 18-19 0.09906796904095216 1108 20-21 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-0.9481586514162252 2316 78-79 -0.8100919333175796 2316 80-81 -0.8179583270588431 2316 82-83 -0.763670036735757 2316 84-85 -1.3412681276178589 2316 86-87 -0.8054456726979033 2316 88-89 -0.5982927431962999 2316 90-91 -0.5974053246343196 2316 92-93 -0.33509745329216756 2316 94-95 -0.6277787231325256 2316 96-97 -0.6505144039343591 2316 98-99 -0.8474521813169211 2316 100-101 -1.2293941808974171 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 12.0 4 43.0 5 84.0 6 199.0 7 501.0 8 1146.0 9 1986.0 10 3221.0 11 4830.0 12 6709.0 13 9246.0 14 11811.0 15 15655.0 16 19790.0 17 24303.0 18 22456.0 19 18756.0 20 18400.0 21 19639.0 22 21477.0 23 23414.0 24 27046.0 25 30878.0 26 36516.0 27 43014.0 28 50499.0 29 59008.0 30 68572.0 31 79322.0 32 92022.0 33 106746.0 34 123886.0 35 139173.0 36 140008.0 37 103860.0 38 29498.0 39 732.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.72564327511291 22.75270942072009 16.237915053125025 20.28373225104197 2 18.381976711132246 23.99088587332516 36.410433991616834 21.216703423925765 3 21.94238283268901 25.918274526945012 30.545309025183332 21.59403361518265 4 16.19048744076984 19.777815841271295 35.36134642389377 28.6703502940651 5 13.856849423289619 38.62468122557047 33.602553444092834 13.915915907047074 6 30.959008078484295 38.45120793348208 16.383110046645825 14.206673941387798 7 27.31512585808978 34.18272347249314 20.733545865443254 17.76860480397383 8 26.309339058607772 35.88022402991003 19.262703567911096 18.547733343571103 9 26.284753651265223 16.745246452096847 20.250328174581494 36.71967172205643 10-11 25.017460807316855 27.736326305204578 26.86863861813768 20.37757426934089 12-13 26.09911761980684 25.087534787810505 27.774432865016834 21.03891472736582 14-15 22.97310666522944 26.19235534108746 26.456740757584928 24.37779723609817 16-17 21.724463929022715 29.49411998790663 26.95015694429114 21.831259138779508 18-19 21.68517694745566 27.54204267116549 30.082894802416522 20.68988557896233 20-21 22.52969840156521 26.532651629812836 30.331093801912218 20.606556166709737 22-23 22.29641732289278 26.617579526040597 30.227241517296164 20.858761633770456 24-25 22.077102719209453 26.93186877446192 30.258357753722866 20.732670752605763 26-27 22.01322083880633 26.940726252734652 30.15700207549301 20.889050832966007 28-29 21.71798931817435 26.986653809392138 30.366598454690475 20.928758417743037 30-31 21.901717212669844 26.827450884822756 30.2842524959143 20.986579406593098 32-33 21.88127788479073 27.017875059894852 30.109076921600312 20.991770133714102 34-35 22.151637982590206 26.952030773547154 29.985048840454514 20.911282403408126 36-37 22.345510274455595 26.746480235637947 29.889993287951317 21.018016201955135 38-39 22.107786367645264 26.96121121684676 29.97374800968861 20.95725440581937 40-41 22.255161218749432 26.88854588030986 29.858334831585946 20.99795806935476 42-43 22.214879840695755 27.129160407696606 29.502960558982476 21.152999192625163 44-45 22.373988589958365 27.14879642297336 29.208606482031808 21.268608505036468 46-47 22.574138491769485 27.4286855504649 28.70692458847429 21.290251369291326 48-49 22.693614352378066 27.733772674769085 28.257157971326404 21.31545500152645 50-51 22.4715397092949 27.987066155455686 28.074224526756993 21.467169608492423 52-53 22.86661336014902 28.030257724461414 27.776645691068797 21.326483224320768 54-55 22.591051718409684 27.878437742257006 27.91487319576212 21.615637343571194 56-57 22.599820886839023 28.158456821250827 27.80968082270972 21.432041469200435 58-59 22.847255881808984 27.874752252110913 27.806754247014382 21.471237619065725 60-61 22.60787774329503 27.922536004161064 28.20275807027369 21.266828182270224 62-63 22.535671925706268 27.751324050185254 28.3179329940349 21.395071030073577 64-65 22.494579657166316 27.920349294164858 28.088986284216087 21.496084764452743 66-67 22.472319083792495 28.162217515114957 27.755115902877396 21.610347498215145 68-69 22.410982448990442 28.030229199705857 27.85643197396438 21.70235637733932 70-71 22.53673886463914 27.9422839524403 27.77486961246995 21.74610757045061 72-73 22.577340330681945 28.000642387614473 27.620120023111188 21.801897258592394 74-75 22.54058296526987 28.34660872159045 27.28893387852503 21.823874434614652 76-77 22.63281389589889 28.196843801082977 27.421674613522896 21.748667689495235 78-79 22.568911049801876 28.1205963215198 27.391420079131002 21.919072549547323 80-81 22.664772186131604 28.280769988947636 27.313521762531366 21.740936062389395 82-83 22.549792325091435 28.328105313014873 27.365243884655975 21.756858477237714 84-85 22.51049778416002 28.26149311253071 27.244661588353964 21.983347514955305 86-87 22.626622230819322 28.149811511877044 27.285224686997495 21.938341570306143 88-89 22.64067918493673 28.087145528765618 27.124280241308814 22.147895044988843 90-91 22.542384268778616 28.23361164986899 27.17953299228545 22.044471089066953 92-93 22.57180457858589 28.183350160488608 27.054374168255556 22.190471092669945 94-95 22.480377774447216 28.154266758905216 26.992622146554456 22.372733320093115 96-97 22.52885927644254 28.14722171669089 26.876383622852053 22.44753538401452 98-99 22.40383621517062 28.233797971147172 26.70872524843849 22.653640565243713 100-101 22.491412581836574 28.355405597464657 26.513259047474904 22.639922773223866 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 585.0 1 439.0 2 305.5 3 328.0 4 307.0 5 323.0 6 542.0 7 1052.5 8 1655.5 9 1981.0 10 1867.5 11 1670.5 12 1685.5 13 1864.0 14 2107.5 15 2321.5 16 2509.5 17 2689.0 18 2969.0 19 3190.0 20 3409.0 21 3859.0 22 4490.5 23 5188.5 24 6153.5 25 7508.5 26 9318.5 27 11452.0 28 13384.5 29 15332.0 30 17574.0 31 20407.0 32 23174.5 33 25603.5 34 28465.0 35 30691.0 36 33269.5 37 36118.0 38 37888.5 39 39000.0 40 40111.0 41 42158.0 42 44359.0 43 45690.0 44 47072.0 45 48507.0 46 48576.0 47 49505.0 48 52142.0 49 52353.5 50 49610.5 51 46809.5 52 44661.5 53 43199.0 54 41718.0 55 38865.0 56 35275.0 57 31661.5 58 28273.0 59 25193.5 60 21693.0 61 17855.0 62 14337.5 63 11226.5 64 8569.5 65 6522.0 66 4770.5 67 3504.5 68 2566.0 69 1670.0 70 1072.0 71 750.0 72 553.5 73 399.0 74 279.5 75 211.0 76 146.5 77 86.5 78 47.5 79 28.0 80 16.5 81 12.5 82 8.5 83 2.5 84 1.5 85 2.5 86 1.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022001355519756183 2 0.005980234218457218 3 0.005463423853899186 4 0.0 5 0.004134482916464249 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.691502603985937E-5 14-15 0.0 16-17 3.691502603985937E-5 18-19 0.0025471367967502966 20-21 8.85960624956625E-4 22-23 0.0 24-25 0.004503633176862843 26-27 0.006275554426776093 28-29 0.007789070494410327 30-31 0.02576668817582184 32-33 3.691502603985937E-4 34-35 0.0038391627081453745 36-37 0.01336323942642909 38-39 0.006533959609055108 40-41 0.007862900546490047 42-43 0.0053526787757796086 44-45 0.003027032135268468 46-47 0.001513516067634234 48-49 7.752155468370467E-4 50-51 0.0022887316144712807 52-53 0.0022887316144712807 54-55 8.85960624956625E-4 56-57 8.121305728769061E-4 58-59 7.752155468370467E-4 60-61 3.691502603985937E-5 62-63 3.691502603985937E-5 64-65 0.005426508827859327 66-67 6.644704687174686E-4 68-69 7.383005207971874E-4 70-71 0.002805541979029312 72-73 0.010299292265120764 74-75 0.005611083958058624 76-77 0.0035438424998265 78-79 0.009265671536004702 80-81 7.383005207971874E-5 82-83 0.004540548202902702 84-85 7.01385494757328E-4 86-87 0.0 88-89 0.001107450781195781 90-91 6.644704687174686E-4 92-93 3.322352343587343E-4 94-95 2.2149015623915624E-4 96-97 1.1074507811957812E-4 98-99 1.4766010415943748E-4 100-101 7.01385494757328E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1354462.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.982304087034734 #Duplication Level Percentage of deduplicated Percentage of total 1 80.97209771845047 50.99809281068538 2 11.278917345506784 14.207444040544775 3 3.4235449180693553 6.468682412563996 4 1.4545502407193278 3.664437022834171 5 0.7704741176539689 2.4263117584636023 6 0.46303615483650534 1.7497850344322448 7 0.3035291579500442 1.3381876007703895 8 0.21836033361277246 1.100226954571679 9 0.15089080122650136 0.855310529410544 >10 0.8811958945522808 10.106045713150651 >50 0.055434057224239754 2.3736215631305244 >100 0.026676584123051864 2.9606764876448364 >500 7.050960407534285E-4 0.2971673693265436 >1k 3.5254802037671426E-4 0.5143252925156043 >5k 2.3503201358447616E-4 0.9396854099551176 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6831 0.5043330857565587 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5865 0.4330132554475504 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3933 0.2903735948295338 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1510 0.11148337864037529 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1506 0.11118805843205641 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.691502603985937E-5 0.0 14-15 0.0 0.0 0.0 7.383005207971874E-5 0.0 16-17 0.0 0.0 0.0 7.383005207971874E-5 0.0 18-19 0.0 0.0 0.0 1.8457513019929685E-4 0.0 20-21 0.0 0.0 0.0 2.584051822790156E-4 0.0 22-23 0.0 0.0 0.0 7.383005207971874E-4 3.691502603985937E-5 24-25 0.0 0.0 0.0 0.001292025911395078 7.383005207971874E-5 26-27 0.0 0.0 0.0 0.0020672414582321246 7.383005207971874E-5 28-29 0.0 0.0 0.0 0.0042452279945838275 7.383005207971874E-5 30-31 0.0 0.0 0.0 0.008342795885008218 7.383005207971874E-5 32-33 0.0 0.0 0.0 0.014286115077425575 7.383005207971874E-5 34-35 0.0 0.0 0.0 0.01982336898340448 7.383005207971874E-5 36-37 0.0 0.0 0.0 0.02846148507673157 7.383005207971874E-5 38-39 0.0 0.0 0.0 0.040052803253247414 7.383005207971874E-5 40-41 0.0 0.0 0.0 0.057956590882579206 7.383005207971874E-5 42-43 0.0 0.0 0.0 0.07656176400666834 7.383005207971874E-5 44-45 0.0 0.0 0.0 0.10133174647941397 7.383005207971874E-5 46-47 0.0 0.0 0.0 0.13558889064440344 7.383005207971874E-5 48-49 0.0 0.0 0.0 0.16512091147629096 7.383005207971874E-5 50-51 0.0 0.0 0.0 0.19801219967780564 7.383005207971874E-5 52-53 0.0 0.0 0.0 0.227581135535733 7.383005207971874E-5 54-55 0.0 0.0 0.0 0.26032476363308826 7.383005207971874E-5 56-57 0.0 0.0 0.0 0.3095324933442208 7.383005207971874E-5 58-59 0.0 0.0 0.0 0.35685755672732056 7.383005207971874E-5 60-61 0.0 0.0 0.0 0.4008602677668329 7.383005207971874E-5 62-63 0.0 0.0 0.0 0.44715171042081653 7.383005207971874E-5 64-65 0.0 0.0 0.0 0.49687625049650713 7.383005207971874E-5 66-67 0.0 0.0 0.0 0.5542791159884884 7.383005207971874E-5 68-69 0.0 0.0 0.0 0.6250083058808589 7.383005207971874E-5 70-71 0.0 0.0 0.0 0.7088423300173796 7.383005207971874E-5 72-73 0.0 0.0 0.0 0.7931193344663785 1.4766010415943748E-4 74-75 0.0 0.0 0.0 0.8840410436025521 2.214901562391562E-4 76-77 0.0 0.0 0.0 0.9865909859412814 2.214901562391562E-4 78-79 0.0 0.0 0.0 1.1049774744511105 2.214901562391562E-4 80-81 0.0 0.0 0.0 1.231374523611589 2.214901562391562E-4 82-83 0.0 0.0 0.0 1.3676647997507496 2.214901562391562E-4 84-85 0.0 0.0 0.0 1.5131469173738354 2.214901562391562E-4 86-87 0.0 0.0 0.0 1.656044983174131 2.214901562391562E-4 88-89 0.0 0.0 0.0 1.809094681135388 2.214901562391562E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4830 0.0 40.036224 1 GTATCAA 7565 0.0 34.731384 1 TATCAAC 9760 0.0 26.864754 2 ATCAACG 9860 0.0 26.687656 3 TCAACGC 9925 0.0 26.511898 4 CAACGCA 10110 0.0 26.120722 5 AACGCAG 10415 0.0 25.400452 6 ACGCAGA 11925 0.0 21.945158 7 GAGTACT 6735 0.0 21.82612 12-13 CGCAGAG 11980 0.0 21.725473 8 AGTACTT 6890 0.0 21.335114 12-13 GTACTTT 6985 0.0 20.774107 14-15 GCAGAGT 12485 0.0 20.35217 9 AGAGTAC 10450 0.0 20.22503 10-11 TGCACCG 335 3.45608E-11 19.849281 5 ACCGTGC 180 1.2345743E-4 18.470177 8 ATACGTC 155 9.630505E-4 18.385738 5 TACTTTT 7930 0.0 18.328453 14-15 TCGCGCT 120 3.094332E-6 17.81184 60-61 CAGAGTA 12220 0.0 17.684212 10-11 >>END_MODULE