FastQCFastQC Report
Thu 26 May 2016
SRR937061_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937061_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405193
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81730.5816282887831067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59150.42093861839619184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39520.2812425054778952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38100.049.1463471
GTATCAA67100.040.8670541
ATCAACG82600.032.9129563
TATCAAC83150.032.7524152
TCAACGC83150.032.6380964
CAACGCA83600.032.5192645
AACGCAG85500.031.789826
ACGCAGA97900.027.6652667
CGCAGAG98750.027.4271378
GCAGAGT104450.025.517339
GTACATG60200.025.1044481
GAGTACT60450.024.08793412-13
TACATGG62700.023.5745722
GTATAGG3700.023.1201571
GTACTTT65200.022.0776414-15
ACATGGG63300.021.6241843
AGTACTT62450.021.45270312-13
AGAGTAC91950.021.39091910-11
CAGAGTA102550.021.28779410-11
CATGGGG30850.018.7955744
Produced by FastQC (version 0.11.5)