##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937061_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1405193 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2869008029502 33.0 31.0 34.0 31.0 34.0 2 32.44522709691836 34.0 31.0 34.0 31.0 34.0 3 32.46093170119692 34.0 31.0 34.0 31.0 34.0 4 36.01266374085268 37.0 35.0 37.0 35.0 37.0 5 36.00783664592693 37.0 35.0 37.0 35.0 37.0 6 36.11007811738316 37.0 37.0 37.0 35.0 37.0 7 36.073304521158306 37.0 37.0 37.0 35.0 37.0 8 36.048660219628196 37.0 37.0 37.0 35.0 37.0 9 37.752441835392005 39.0 38.0 39.0 35.0 39.0 10-11 37.652251327753554 39.0 38.0 39.0 35.0 39.0 12-13 37.5796388823457 39.0 37.5 39.0 35.0 39.0 14-15 38.872276619652965 40.0 38.0 41.0 36.0 41.0 16-17 38.793203851712896 40.0 38.0 41.0 36.0 41.0 18-19 38.746752937141025 40.0 38.0 41.0 35.0 41.0 20-21 38.65259932265532 40.0 38.0 41.0 35.0 41.0 22-23 38.52507342407769 40.0 38.0 41.0 34.5 41.0 24-25 38.65015730935181 40.0 38.0 41.0 35.0 41.0 26-27 38.61226322647494 40.0 38.0 41.0 35.0 41.0 28-29 38.52055731846088 40.0 38.0 41.0 35.0 41.0 30-31 38.340851754883495 40.0 38.0 41.0 34.0 41.0 32-33 38.135000672505484 40.0 38.0 41.0 34.0 41.0 34-35 37.95645793851806 40.0 38.0 41.0 34.0 41.0 36-37 37.778917913767 40.0 37.5 41.0 33.0 41.0 38-39 37.621793589919676 40.0 37.0 41.0 33.0 41.0 40-41 37.430200335469934 40.0 37.0 41.0 33.0 41.0 42-43 37.187088179346176 40.0 37.0 41.0 32.5 41.0 44-45 36.915179978835646 39.5 36.0 41.0 31.5 41.0 46-47 36.541667585876105 39.0 36.0 41.0 31.0 41.0 48-49 36.2740481200803 39.0 36.0 41.0 30.0 41.0 50-51 35.598265149342474 38.5 35.0 40.0 29.0 40.5 52-53 35.79492105354923 38.5 35.5 40.0 30.0 40.5 54-55 36.183708216593736 39.0 36.0 40.5 30.0 41.0 56-57 36.086056861939966 39.0 35.5 41.0 30.0 41.0 58-59 35.84610903982585 39.0 35.0 41.0 29.5 41.0 60-61 35.57425670352756 38.5 35.0 40.0 29.0 41.0 62-63 35.23860708102019 38.0 35.0 40.0 28.0 41.0 64-65 34.90694267620178 37.5 35.0 40.0 28.0 41.0 66-67 34.53593492139514 37.0 34.0 40.0 27.5 41.0 68-69 34.13943315971542 36.5 34.0 39.0 26.0 41.0 70-71 33.75009091277853 36.0 34.0 39.0 26.0 40.5 72-73 33.34409223501683 35.5 34.0 38.5 26.0 40.0 74-75 32.90512121822412 35.0 33.0 37.5 25.5 39.5 76-77 32.47773152869392 35.0 33.0 37.0 24.5 39.0 78-79 32.06328347778562 35.0 32.5 36.5 23.5 39.0 80-81 31.632865022811814 35.0 32.0 36.0 21.5 38.5 82-83 31.221085644463074 35.0 31.5 35.5 20.0 37.0 84-85 30.85376136943466 34.5 31.0 35.0 18.5 37.0 86-87 30.518496391598877 34.0 31.0 35.0 17.5 36.0 88-89 30.186873618072394 34.0 31.0 35.0 13.0 36.0 90-91 29.82650176879617 34.0 30.5 35.0 7.0 36.0 92-93 29.476284040697614 34.0 30.0 35.0 2.0 35.0 94-95 29.090554464760356 34.0 30.0 35.0 2.0 35.0 96-97 28.613554508170765 34.0 29.0 35.0 2.0 35.0 98-99 27.99632328085893 34.0 29.0 35.0 2.0 35.0 100-101 25.367338863771735 31.5 23.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3193726813438502 1101 2 0.27952800677834944 1101 3 0.21244578165946137 1101 4 0.28105163748828943 1101 5 0.30110678873009533 1101 6 0.1716996084319149 1101 7 0.15535973852198026 1101 8 0.07975102509487897 1101 9 0.24803296450958356 1101 10-11 0.24023464933465277 1101 12-13 0.3446929006432242 1101 14-15 0.3028408780144005 1101 16-17 0.451663840380057 1101 18-19 0.5265807817140171 1101 20-21 0.5282607992749533 1101 22-23 0.5075328401029466 1101 24-25 0.5142145416808432 1101 26-27 0.49595316470788475 1101 28-29 0.4406082394458579 1101 30-31 0.28261422250932355 1101 32-33 0.3316354506471981 1101 34-35 0.4802618770848994 1101 36-37 0.4278879786338976 1101 38-39 0.36602837011958655 1101 40-41 0.17951149774220454 1101 42-43 0.12756537039384597 1101 44-45 0.11473744374166728 1101 46-47 0.0922029354052043 1101 48-49 0.12398603369170047 1101 50-51 0.11485852793487084 1101 52-53 0.11600820893381325 1101 54-55 0.16762427132174196 1101 56-57 0.10207015992374124 1101 58-59 0.09541681111399214 1101 60-61 0.28861752444040434 1101 62-63 0.2354666005154371 1101 64-65 0.24333595611121694 1101 66-67 0.1457268648601655 1101 68-69 0.14567533279757328 1101 70-71 0.16039521278716506 1101 72-73 0.06729201748677838 1101 74-75 0.07903076817168397 1101 76-77 0.20608569673890997 1101 78-79 0.07809186677548041 1101 80-81 0.2287851414447779 1101 82-83 0.17054102072184918 1101 84-85 0.08389329329925843 1101 86-87 0.14602726040845582 1101 88-89 0.15152533493317932 1101 90-91 0.12060837076240105 1101 92-93 0.13073135150938242 1101 94-95 0.21354698125585614 1101 96-97 0.17963985162991847 1101 98-99 0.047931978617391735 1101 100-101 0.35552053684122953 1102 1 0.30977191174308416 1102 2 0.36548279273313256 1102 3 0.27982432903800714 1102 4 0.262138698575356 1102 5 0.26323281085611683 1102 6 0.21082418755649002 1102 7 0.24263246579470632 1102 8 0.24214640749026017 1102 9 0.3859742824508956 1102 10-11 0.3130485021485043 1102 12-13 0.38338457933490133 1102 14-15 0.3532497284232434 1102 16-17 0.45710395582016616 1102 18-19 0.46664812178135406 1102 20-21 0.4539991350132837 1102 22-23 0.46422355679366234 1102 24-25 0.22253103999734947 1102 26-27 0.4098059785606978 1102 28-29 0.3068756757132931 1102 30-31 0.2980831979783005 1102 32-33 0.5391101981219464 1102 34-35 0.36348939031240945 1102 36-37 0.3216301623760742 1102 38-39 0.4757301498213664 1102 40-41 0.4705648887956002 1102 42-43 0.5036981265266078 1102 44-45 0.40691637592060204 1102 46-47 0.5966040898063554 1102 48-49 0.45943095913663257 1102 50-51 0.4362245693009186 1102 52-53 0.4499187428443392 1102 54-55 0.6489999326974001 1102 56-57 0.6102952681488532 1102 58-59 0.6119295576267447 1102 60-61 0.5492620650849531 1102 62-63 0.6186412036900393 1102 64-65 0.5698150325902915 1102 66-67 0.5061020452353517 1102 68-69 0.5882631153853524 1102 70-71 0.5491301815221377 1102 72-73 0.3953872555820155 1102 74-75 0.35930974845067 1102 76-77 0.4743492850025035 1102 78-79 0.5272164459000592 1102 80-81 0.562782255441892 1102 82-83 0.7239946041754308 1102 84-85 0.4976450970510591 1102 86-87 0.3284082127894088 1102 88-89 0.39493081833866484 1102 90-91 0.37836209851613134 1102 92-93 0.42831672909476026 1102 94-95 0.5457595834684632 1102 96-97 0.4662680382581037 1102 98-99 0.2946852253706389 1102 100-101 0.4451597864804775 1103 1 0.18039787741727764 1103 2 0.26423002620705205 1103 3 0.2111613093138942 1103 4 0.21892911073553734 1103 5 0.26381966237543963 1103 6 0.2650918063257208 1103 7 0.16024413601088128 1103 8 0.1583352133253655 1103 9 0.25192380987952845 1103 10-11 0.30951194224537915 1103 12-13 0.3073088008796958 1103 14-15 0.318667108470855 1103 16-17 0.29211961540174514 1103 18-19 0.3851152923385541 1103 20-21 0.5326458687725548 1103 22-23 0.4567272802941744 1103 24-25 0.20733623480253982 1103 26-27 0.3240499339622076 1103 28-29 0.35862913126096174 1103 30-31 0.38282603284974925 1103 32-33 0.5299996623554577 1103 34-35 0.3862546636243138 1103 36-37 0.5253862348138227 1103 38-39 0.5989525437190011 1103 40-41 0.5487479597439417 1103 42-43 0.425684596648118 1103 44-45 0.3894784226627124 1103 46-47 0.48236779020027853 1103 48-49 0.513795319320927 1103 50-51 0.44237941124353597 1103 52-53 0.5990480701087151 1103 54-55 0.5707469852161537 1103 56-57 0.7160419865965366 1103 58-59 0.6123148529316538 1103 60-61 0.6420507233720016 1103 62-63 0.7391311745980218 1103 64-65 0.7050442192664192 1103 66-67 0.6429962978209218 1103 68-69 0.7418542402304951 1103 70-71 0.6342268533390651 1103 72-73 0.5098582401173104 1103 74-75 0.5651372007411339 1103 76-77 0.45043912228204874 1103 78-79 0.4055217311828372 1103 80-81 0.5298084661015459 1103 82-83 0.4466770557003237 1103 84-85 0.4872950184051632 1103 86-87 0.46979850524079225 1103 88-89 0.501895365045975 1103 90-91 0.5562733191283122 1103 92-93 0.4868951174802234 1103 94-95 0.5796437164844264 1103 96-97 0.4697145381676293 1103 98-99 0.42402292946718845 1103 100-101 0.5619539569402434 1104 1 0.29705866754047605 1104 2 0.2559072618810063 1104 3 0.3208377836684875 1104 4 0.3569182452697959 1104 5 0.3261355818439924 1104 6 0.26036075028454064 1104 7 0.24797917582226603 1104 8 0.23135521286927485 1104 9 0.30415312488506174 1104 10-11 0.2912205428737309 1104 12-13 0.2678096578025304 1104 14-15 0.3804870301286343 1104 16-17 0.3905631207686966 1104 18-19 0.3632203438854873 1104 20-21 0.5323502874069916 1104 22-23 0.3944490759553574 1104 24-25 0.325312230438108 1104 26-27 0.2821194806614358 1104 28-29 0.39355509558419755 1104 30-31 0.23988437552415576 1104 32-33 0.4369481048960182 1104 34-35 0.39908131611710473 1104 36-37 0.3410224013427836 1104 38-39 0.35795374183219053 1104 40-41 0.526307019005813 1104 42-43 0.534413368731208 1104 44-45 0.44334264468729856 1104 46-47 0.4225292284974458 1104 48-49 0.6324624666362197 1104 50-51 0.48763712837305206 1104 52-53 0.6118957729064789 1104 54-55 0.6824096435539175 1104 56-57 0.6943544713569878 1104 58-59 0.7460307755577489 1104 60-61 0.7434811793040623 1104 62-63 0.8595748799428691 1104 64-65 0.9140877811183614 1104 66-67 0.6809280702741418 1104 68-69 0.6443746634117957 1104 70-71 0.7813531358727488 1104 72-73 0.8221162712472037 1104 74-75 0.6877267215484864 1104 76-77 0.7261897849281098 1104 78-79 0.9012436952464569 1104 80-81 0.820300481896286 1104 82-83 0.800527858155494 1104 84-85 0.8550815612960356 1104 86-87 0.8543937166472517 1104 88-89 0.8516084669312072 1104 90-91 0.568273747151185 1104 92-93 0.5468477016682876 1104 94-95 0.8473163245996709 1104 96-97 0.8463957120027992 1104 98-99 0.6954652963634764 1104 100-101 0.7471865540391605 1105 1 0.310905297373111 1105 2 0.36939342348939874 1105 3 0.274561828674841 1105 4 0.35632417752593426 1105 5 0.376635001506628 1105 6 0.2888119268865097 1105 7 0.20708552950951997 1105 8 0.32134045728833627 1105 9 0.4262244349829274 1105 10-11 0.3302953716101342 1105 12-13 0.33547891676480646 1105 14-15 0.23524295890641156 1105 16-17 0.3974745360229619 1105 18-19 0.39744651801599673 1105 20-21 0.4176068807351285 1105 22-23 0.41208345975423555 1105 24-25 0.16231386343788756 1105 26-27 0.17043382945699648 1105 28-29 0.19269978449042213 1105 30-31 0.12048042044263951 1105 32-33 0.19615020509483827 1105 34-35 0.10461914422739937 1105 36-37 0.2650439745147182 1105 38-39 0.2543561993870114 1105 40-41 0.25255666659274567 1105 42-43 0.24451700563407286 1105 44-45 0.09898427446501046 1105 46-47 0.08882059813024767 1105 48-49 0.04645948988999038 1105 50-51 0.0641199178002907 1105 52-53 0.10663886835641279 1105 54-55 0.246114026657132 1105 56-57 0.3575111953983381 1105 58-59 0.2588032320507523 1105 60-61 0.14517624039509514 1105 62-63 0.3040135271160551 1105 64-65 0.3115419857266417 1105 66-67 0.27161905618859805 1105 68-69 0.359599731789082 1105 70-71 0.32739567441850426 1105 72-73 0.2816496397303041 1105 74-75 0.2536348305274245 1105 76-77 0.23766838201287044 1105 78-79 0.23535279500956108 1105 80-81 0.33610643685332064 1105 82-83 0.29744081121224397 1105 84-85 0.34706576586770055 1105 86-87 0.2179192386762736 1105 88-89 0.2612062766611025 1105 90-91 0.37475595074890933 1105 92-93 0.32001085152714026 1105 94-95 0.3622215666754123 1105 96-97 0.04132178764071526 1105 98-99 0.1462061876298577 1105 100-101 0.4192762628452762 1106 1 0.16975705175206457 1106 2 0.24943864617647193 1106 3 0.2917036535275841 1106 4 0.251771378172279 1106 5 0.24025833598652468 1106 6 0.17679511769905787 1106 7 0.17761412299329038 1106 8 0.2516639221567587 1106 9 0.3062169956375911 1106 10-11 0.27131189548936163 1106 12-13 0.39522799217949967 1106 14-15 0.41901911993757324 1106 16-17 0.37269038369503704 1106 18-19 0.3737546029045191 1106 20-21 0.4095852533050035 1106 22-23 0.48790945373343675 1106 24-25 0.44541728367023836 1106 26-27 0.42623865196596 1106 28-29 0.5397272460738023 1106 30-31 0.5206205414453251 1106 32-33 0.45671727519266625 1106 34-35 0.4486145392992995 1106 36-37 0.3575545002551266 1106 38-39 0.35661586720291183 1106 40-41 0.36179672013469855 1106 42-43 0.32580685212757743 1106 44-45 0.4486005082364386 1106 46-47 0.6144043467603666 1106 48-49 0.49956794967886253 1106 50-51 0.45064123725751415 1106 52-53 0.5125791456120155 1106 54-55 0.3634457014697503 1106 56-57 0.46898371914958403 1106 58-59 0.42494427830535386 1106 60-61 0.5216664425906359 1106 62-63 0.44766555240450145 1106 64-65 0.4657757464890224 1106 66-67 0.49782781648436014 1106 68-69 0.43023051628004794 1106 70-71 0.5118554419589572 1106 72-73 0.5179191813439772 1106 74-75 0.6327111384789958 1106 76-77 0.6022482989801787 1106 78-79 0.521293965519888 1106 80-81 0.45575224135585657 1106 82-83 0.5860457473112 1106 84-85 0.6243306998868441 1106 86-87 0.6842502015515386 1106 88-89 0.6948204519470096 1106 90-91 0.8260009078593491 1106 92-93 0.6595736366687142 1106 94-95 0.4619811211679483 1106 96-97 0.5341100889368668 1106 98-99 0.6449862949804093 1106 100-101 0.6224407177065814 1107 1 0.14248899857781794 1107 2 0.23870238359978657 1107 3 0.21717090403164008 1107 4 0.21082556726221924 1107 5 0.21956588954213174 1107 6 0.2076190267042719 1107 7 0.12712783970184915 1107 8 0.21054637353728367 1107 9 0.26644962410271233 1107 10-11 0.28065566622625227 1107 12-13 0.2924534189919683 1107 14-15 0.3108764154616992 1107 16-17 0.40454135502227473 1107 18-19 0.3000323845773778 1107 20-21 0.32446513959693846 1107 22-23 0.3500447943796061 1107 24-25 0.4038602989736688 1107 26-27 0.30164028804206566 1107 28-29 0.29030832149887686 1107 30-31 0.41973331256959057 1107 32-33 0.4704267247325902 1107 34-35 0.43136817819119955 1107 36-37 0.4379734269546347 1107 38-39 0.3558099393129197 1107 40-41 0.3552520970122188 1107 42-43 0.4825522136159819 1107 44-45 0.5172924051201591 1107 46-47 0.46714988388156087 1107 48-49 0.5519052821991863 1107 50-51 0.5449776462892828 1107 52-53 0.5936756395424183 1107 54-55 0.612006468645113 1107 56-57 0.5704174988593209 1107 58-59 0.6906478328156069 1107 60-61 0.5915318214723513 1107 62-63 0.597456244858023 1107 64-65 0.6485859348906047 1107 66-67 0.5726006446751271 1107 68-69 0.5925276090616123 1107 70-71 0.648602212758874 1107 72-73 0.756253056447818 1107 74-75 0.68127732335941 1107 76-77 0.5048068013423688 1107 78-79 0.5189641752948475 1107 80-81 0.5657174901418323 1107 82-83 0.6198591318046631 1107 84-85 0.30530488824026136 1107 86-87 0.3648584292396251 1107 88-89 0.461651809765538 1107 90-91 0.4206012972259181 1107 92-93 0.28276711530985565 1107 94-95 0.4038633098074804 1107 96-97 0.19711431616320851 1107 98-99 0.22868912996277757 1107 100-101 0.2508435145171468 1108 1 -0.03877583033370513 1108 2 0.08345775967749347 1108 3 0.028090104486793166 1108 4 0.22868687869005555 1108 5 0.27568055049935936 1108 6 0.12513975331490457 1108 7 0.018909305756743322 1108 8 0.18701235977657404 1108 9 0.1718626476771803 1108 10-11 0.08007284570001616 1108 12-13 0.13497311572043458 1108 14-15 0.25691058973557546 1108 16-17 0.26312146111312273 1108 18-19 0.3401176254195022 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2316 72-73 -1.5971511151381712 2316 74-75 -1.736085765126667 2316 76-77 -2.0854618129904168 2316 78-79 -2.0420161396961625 2316 80-81 -2.120854108194475 2316 82-83 -1.9599959507242168 2316 84-85 -1.8819534861838854 2316 86-87 -2.0711903704455388 2316 88-89 -1.964596926643626 2316 90-91 -1.9877063784614393 2316 92-93 -2.4224979108107867 2316 94-95 -1.9278649737924596 2316 96-97 -2.0033895768540546 2316 98-99 -2.2672982457037385 2316 100-101 -1.9897634720791437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2709.0 3 724.0 4 696.0 5 633.0 6 705.0 7 852.0 8 1031.0 9 1201.0 10 1524.0 11 2127.0 12 3083.0 13 4097.0 14 4783.0 15 6475.0 16 8931.0 17 13189.0 18 11367.0 19 5766.0 20 5434.0 21 5296.0 22 5734.0 23 6113.0 24 7024.0 25 7938.0 26 9281.0 27 11238.0 28 13601.0 29 17034.0 30 21752.0 31 29047.0 32 39223.0 33 56442.0 34 85363.0 35 137121.0 36 225180.0 37 335393.0 38 276951.0 39 40121.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.95420297777806 18.74468368315143 14.792297951487429 23.508815387583077 2 14.903241877513256 19.906764883811963 41.153624426177004 24.036368812497777 3 18.552249396984102 24.716712419244978 30.23833807466223 26.49270010910869 4 12.373720657602812 16.76172539238861 37.8596376593417 33.00491629066688 5 13.6214084065803 36.82533579685225 34.43550884837611 15.117746948191343 6 30.886390996767172 36.30652738651807 18.032593149472717 14.774488467242042 7 27.53744186741714 33.03589095345265 21.2802620396903 18.146405139439906 8 25.76861604616146 34.20013028295607 20.341441014925586 19.689812655956885 9 26.108881085579117 16.506776327450478 20.748823512243945 36.63551907472646 10-11 24.167546623474934 26.44664365432805 27.85866736992032 21.527142352276694 12-13 25.439659320976933 24.037462163385833 28.33286111720167 22.190017398435565 14-15 22.499916365523234 25.01304341946142 27.07319191158519 25.413848303430154 16-17 21.49671770248427 28.488904674222276 27.41798526791847 22.596392355374988 18-19 21.346120174862953 26.737632972855245 30.30695679529903 21.609290056982775 20-21 22.073576810034577 25.811313207662657 30.47408883066914 21.641021151633623 22-23 21.840344746599865 25.79140837953441 30.451465742407958 21.91678113145777 24-25 21.621453324380692 26.053581359997697 30.36732526954895 21.95764004607267 26-27 21.46339380547505 26.2161945783229 30.258416551468926 22.06199506473312 28-29 21.271118983662753 26.20271591170707 30.450121996479414 22.07604310815076 30-31 21.41090227463416 26.01777834076885 30.41781449238645 22.153504892210535 32-33 21.319907855811568 26.222486797149706 30.279022964170654 22.17858238286807 34-35 21.699411290623882 26.328385612647377 29.978668403083564 21.993534693645177 36-37 21.71160829864652 26.02589110534994 30.085084397659255 22.177416198344286 38-39 21.550941633222234 26.226033960495776 30.09618261994895 22.12684178633304 40-41 21.65293308463677 26.199497150925176 30.084515080846547 22.063054683591506 42-43 21.531561856627523 26.2545785525547 29.84611366552495 22.367745925292827 44-45 21.630943222745916 26.469709143156848 29.62194517052106 22.27740246357618 46-47 21.7930561851646 26.53251190405873 29.27113926699037 22.403292643786298 48-49 21.89371746210562 26.756730502704794 28.922347428832502 22.427204606357083 50-51 21.783093140942206 26.880862628834617 28.828175204402527 22.507869025820654 52-53 22.037418560545408 26.98021968637582 28.578728067834486 22.40363368524429 54-55 21.78955125470271 26.89127001042932 28.734138524530955 22.585040210337016 56-57 21.787053708039483 27.174439043830372 28.58905201431835 22.449455233811797 58-59 21.97891310435526 26.877198775535327 28.61839237595321 22.5254957441562 60-61 21.9290426735299 26.7501370832039 28.983427966112252 22.33739227715395 62-63 21.740815200953616 26.861387371679683 29.09000658280997 22.307790844556727 64-65 21.733226822850412 26.873519987617268 28.861090997076893 22.53216219245543 66-67 21.657713478036218 27.167020381670586 28.551028193292083 22.62423794700111 68-69 21.560555000017793 27.07222900334153 28.77324213815216 22.593973858488518 70-71 21.7304080724048 26.946345185487296 28.611333837983192 22.711912904124713 72-73 21.80140560326743 26.96524852502047 28.566720960100554 22.666624911611546 74-75 21.68152552530324 27.176337617562446 28.43655293459492 22.70558392253939 76-77 21.781129512910503 27.070789685264096 28.39773369935249 22.750347102472908 78-79 21.848085453933987 27.094221040700226 28.356243115943965 22.701450389421822 80-81 21.68687264555762 27.314397058074967 28.310973806088814 22.6877564902786 82-83 21.72925287352231 27.296522160663834 28.347269984745253 22.6269549810686 84-85 21.770957702922235 27.114044281059996 28.15681822630209 22.958179789715675 86-87 21.737979313294765 27.046126954280147 28.43461154956703 22.78128218285806 88-89 21.825276935882258 27.079594623303503 28.178904369802503 22.916224071011737 90-91 21.693923845030895 27.16393667821867 28.291449310331817 22.850690166418616 92-93 21.806891834129317 27.126902895686456 28.241244446532427 22.824960823651804 94-95 21.70970129602084 27.03644658273253 28.19475186289651 23.059100258350117 96-97 21.70156491387155 27.024578183592407 28.19926895002048 23.07458795251556 98-99 21.63414380670502 27.00187492259004 28.04475612481831 23.31922514588663 100-101 21.675043950931574 26.928617422402002 28.13927470907313 23.257063917593293 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 537.0 1 401.0 2 324.5 3 383.5 4 317.0 5 302.5 6 571.5 7 1353.0 8 2458.5 9 2931.5 10 2389.5 11 1835.5 12 1816.0 13 1930.5 14 2071.0 15 2177.0 16 2220.0 17 2304.0 18 2489.5 19 2716.0 20 3010.0 21 3367.5 22 3871.5 23 4633.0 24 5583.0 25 6983.5 26 8874.0 27 10962.0 28 12850.5 29 14686.0 30 16874.5 31 19689.0 32 22594.5 33 25205.0 34 28387.5 35 30955.0 36 33495.5 37 36452.0 38 38622.0 39 39730.5 40 40787.5 41 42992.0 42 44982.5 43 46838.0 44 48907.5 45 50396.0 46 50581.0 47 51941.0 48 55375.0 49 55332.5 50 52283.0 51 50280.5 52 48347.5 53 47088.0 54 45712.0 55 42340.0 56 38568.0 57 34437.5 58 30618.0 59 27150.0 60 23392.0 61 19140.0 62 14940.5 63 11857.0 64 9238.5 65 7063.0 66 5002.5 67 3593.5 68 2603.5 69 1639.5 70 1102.5 71 773.5 72 540.0 73 375.5 74 291.0 75 206.0 76 122.5 77 69.5 78 40.5 79 29.0 80 25.0 81 19.5 82 16.5 83 12.0 84 11.5 85 10.0 86 6.5 87 6.5 88 6.0 89 4.0 90 2.0 91 0.5 92 2.0 93 3.0 94 1.5 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022772672508331596 2 0.010176538027160682 3 0.012240311473228233 4 0.019356771632081857 5 0.04967289190879829 6 0.03857121406098664 7 0.01530038934153529 8 0.03970984768640322 9 0.026757890197289625 10-11 0.02095797516782392 12-13 0.033376198145023496 14-15 0.019214442428904785 16-17 0.016510187568540405 18-19 0.014731072528827 20-21 0.012204729172433966 22-23 0.007329953963619233 24-25 0.0015300389341535291 26-27 3.558230079426812E-5 28-29 0.002099355746861819 30-31 0.0 32-33 2.8465840635414496E-4 34-35 3.558230079426812E-5 36-37 0.0 38-39 1.0674690238280437E-4 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 3.202407071484131E-4 50-51 0.0 52-53 5.693168127082899E-4 54-55 0.0013877097309764567 56-57 2.1349380476560874E-4 58-59 5.337345119140217E-4 60-61 0.0017435327389191378 62-63 0.0012809628285936523 64-65 7.472283166796305E-4 66-67 0.007614612369973378 68-69 0.010532361035103362 70-71 0.018396049510636617 72-73 0.012347058375611038 74-75 0.013877097309764564 76-77 0.024800863653604878 78-79 0.01604761765821492 80-81 0.03803747954907262 82-83 0.02732720700999792 84-85 0.013556856602616154 86-87 0.009678385816040929 88-89 0.009215815905715442 90-91 0.01722183358442577 92-93 0.02330640702024562 94-95 0.008753245995389956 96-97 0.016189946861391993 98-99 0.02384014153215964 100-101 0.03568904769665092 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1405193.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.316020296374894 #Duplication Level Percentage of deduplicated Percentage of total 1 78.87626966623934 47.57502682091231 2 12.417353598547672 14.979307033545302 3 3.816491977377692 6.905868227053944 4 1.6502485824178303 3.9814570796471096 5 0.8749897933777718 2.6387951068247277 6 0.5328890448831235 1.9285047868131784 7 0.34700058257753935 1.4650785927120542 8 0.23713510416790035 1.144243660477924 9 0.1836180498297392 0.9967599018280192 >10 0.964041997782759 10.614462811690654 >50 0.06908794578507158 2.8630125239087074 >100 0.029459626673195065 3.1433581266189137 >500 8.248510318939531E-4 0.3109483223825091 >1k 3.535075850974085E-4 0.45188787056182683 >5k 2.356717233982723E-4 1.0012891350228086 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8173 0.5816282887831067 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5915 0.42093861839619184 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3952 0.2812425054778952 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 1.0674690238280436E-4 16-17 0.0 0.0 0.0 3.558230079426812E-5 2.134938047656087E-4 18-19 0.0 0.0 0.0 7.116460158853624E-5 2.134938047656087E-4 20-21 0.0 0.0 0.0 7.116460158853624E-5 2.134938047656087E-4 22-23 0.0 0.0 0.0 3.558230079426812E-4 2.8465840635414496E-4 24-25 0.0 0.0 0.0 7.116460158853624E-4 2.8465840635414496E-4 26-27 0.0 0.0 0.0 0.001174215926210848 2.8465840635414496E-4 28-29 0.0 0.0 0.0 0.0025263433563930365 2.8465840635414496E-4 30-31 0.0 0.0 0.0 0.005942244232642776 3.202407071484131E-4 32-33 0.0 7.116460158853624E-5 0.0 0.009500474312069587 3.558230079426812E-4 34-35 0.0 7.116460158853624E-5 0.0 0.014624325626444197 3.914053087369493E-4 36-37 0.0 7.116460158853624E-5 0.0 0.021811950386886356 4.269876095312174E-4 38-39 0.0 7.116460158853624E-5 0.0 0.03134800699975021 4.981522111197537E-4 40-41 0.0 7.116460158853624E-5 0.0 0.04632815563413709 4.981522111197537E-4 42-43 0.0 7.116460158853624E-5 0.0 0.06120155736614116 4.981522111197537E-4 44-45 0.0 7.116460158853624E-5 0.0 0.08023808829107461 4.981522111197537E-4 46-47 0.0 7.116460158853624E-5 0.0 0.11098119617732227 4.981522111197537E-4 48-49 0.0 7.116460158853624E-5 0.0 0.13720535186269786 6.404814142968262E-4 50-51 0.0 7.116460158853624E-5 0.0 0.16374974825522187 6.404814142968262E-4 52-53 0.0 7.116460158853624E-5 0.0 0.1909346260620427 6.760637150910943E-4 54-55 0.0 7.116460158853624E-5 0.0 0.220432353420491 7.116460158853624E-4 56-57 0.0 7.116460158853624E-5 0.0 0.2671163320625708 7.116460158853624E-4 58-59 0.0 7.116460158853624E-5 0.0 0.31276842398161675 7.116460158853624E-4 60-61 0.0 7.116460158853624E-5 0.0 0.3583493512990742 7.828106174738986E-4 62-63 0.0 7.116460158853624E-5 0.0 0.40585172285942217 7.828106174738986E-4 64-65 0.0 7.116460158853624E-5 0.0 0.45541786786583766 7.828106174738986E-4 66-67 0.0 7.116460158853624E-5 0.0 0.5153384624033852 7.828106174738986E-4 68-69 0.0 7.116460158853624E-5 0.0 0.5870723808046296 7.828106174738986E-4 70-71 0.0 7.116460158853624E-5 0.0 0.6712245221830738 7.828106174738986E-4 72-73 0.0 7.116460158853624E-5 0.0 0.7566576263901115 8.539752190624348E-4 74-75 0.0 7.116460158853624E-5 0.0 0.8497409252679169 8.539752190624348E-4 76-77 0.0 7.116460158853624E-5 0.0 0.9568080683578697 8.539752190624348E-4 78-79 0.0 7.116460158853624E-5 0.0 1.0735180149630692 8.539752190624348E-4 80-81 0.0 7.116460158853624E-5 0.0 1.2114705951424467 9.607221214452393E-4 82-83 0.0 7.116460158853624E-5 0.0 1.3536218868155476 0.0011386336254165799 84-85 0.0 7.116460158853624E-5 0.0 1.50520248819913 0.0011386336254165799 86-87 0.0 7.116460158853624E-5 0.0 1.6625118400105894 0.001174215926210848 88-89 0.0 7.116460158853624E-5 0.0 1.8314566041817741 0.001209798227005116 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3810 0.0 49.146347 1 GTATCAA 6710 0.0 40.867054 1 ATCAACG 8260 0.0 32.912956 3 TATCAAC 8315 0.0 32.752415 2 TCAACGC 8315 0.0 32.638096 4 CAACGCA 8360 0.0 32.519264 5 AACGCAG 8550 0.0 31.78982 6 ACGCAGA 9790 0.0 27.665266 7 CGCAGAG 9875 0.0 27.427137 8 GCAGAGT 10445 0.0 25.51733 9 GTACATG 6020 0.0 25.104448 1 GAGTACT 6045 0.0 24.087934 12-13 TACATGG 6270 0.0 23.574572 2 GTATAGG 370 0.0 23.120157 1 GTACTTT 6520 0.0 22.07764 14-15 ACATGGG 6330 0.0 21.624184 3 AGTACTT 6245 0.0 21.452703 12-13 AGAGTAC 9195 0.0 21.390919 10-11 CAGAGTA 10255 0.0 21.287794 10-11 CATGGGG 3085 0.0 18.795574 4 >>END_MODULE