FastQCFastQC Report
Thu 26 May 2016
SRR937061_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937061_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405193
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71520.5089692305612112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60690.4318979670408264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40800.29035157448122784No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16050.11421918554960066No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14640.10418497672561705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55450.038.295171
GTATCAA84000.031.726461
TATCAAC105800.025.0931972
ATCAACG106850.024.7577133
TCAACGC107350.024.5981584
CAACGCA109550.024.1101785
AACGCAG112400.023.5833726
GAGTACT74200.021.21824512-13
AGTACTT75850.021.0384412-13
ACGCAGA128250.020.3724547
CGCAGAG129100.019.9071488
GTACTTT78500.019.7840714-15
AGAGTAC110500.019.47484210-11
GCAGAGT134650.018.7690949
TACTTTT86650.018.30692714-15
GTACATG59400.017.3544671
TAGGACG1650.001465472717.2701914
CAGAGTA131900.017.12551910-11
ACTTTTT93300.016.82422616-17
TATACGC2002.7639748E-416.6225593
Produced by FastQC (version 0.11.5)