##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937061_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1405193 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.21860271151365 31.0 31.0 33.0 30.0 34.0 2 31.377135382826417 31.0 31.0 34.0 30.0 34.0 3 31.42766509653834 31.0 31.0 34.0 30.0 34.0 4 35.247134023582525 37.0 35.0 37.0 33.0 37.0 5 35.07886888135651 35.0 35.0 37.0 32.0 37.0 6 35.18259698133993 37.0 35.0 37.0 32.0 37.0 7 35.118568765998695 37.0 35.0 37.0 32.0 37.0 8 35.07346392986586 37.0 35.0 37.0 32.0 37.0 9 36.56505974624126 38.0 35.0 39.0 32.0 39.0 10-11 36.38781220800274 38.0 35.0 39.0 32.0 39.0 12-13 36.22074263108341 38.0 35.0 39.0 32.0 39.0 14-15 37.07989934478751 38.0 36.0 40.0 32.0 41.0 16-17 36.90853569580833 38.0 36.0 40.0 31.0 41.0 18-19 37.10569971527043 38.5 36.0 40.0 31.0 41.0 20-21 37.13299027251061 39.0 36.0 40.0 31.0 41.0 22-23 36.98718894842203 39.0 36.0 40.0 31.0 41.0 24-25 36.801873835124425 38.5 36.0 40.0 31.0 41.0 26-27 36.45991938473932 38.0 35.0 40.0 30.0 41.0 28-29 36.26836455917443 38.0 35.0 40.0 30.0 41.0 30-31 36.19325708283489 38.0 35.0 40.0 30.0 41.0 32-33 35.925548661287095 38.0 34.5 40.0 29.5 41.0 34-35 35.67207956487116 38.0 34.0 40.0 29.0 41.0 36-37 35.396049510636615 38.0 34.0 40.0 27.0 41.0 38-39 35.12832934692956 38.0 34.0 40.0 27.0 41.0 40-41 34.803084344997444 38.0 33.0 40.0 26.0 41.0 42-43 34.38458097926762 37.0 33.0 40.0 24.5 41.0 44-45 33.96289228597068 37.0 33.0 40.0 24.0 41.0 46-47 33.42055575283965 37.0 32.0 39.5 22.5 40.5 48-49 32.87374296626869 37.0 31.0 39.0 19.5 40.0 50-51 32.40314141900792 36.0 31.0 39.0 17.0 40.0 52-53 31.989110036841915 36.0 30.0 39.0 14.5 40.0 54-55 31.533723481400777 35.5 30.0 39.0 11.5 40.0 56-57 31.05664061805033 35.0 29.0 38.0 8.5 40.0 58-59 30.49793800566897 34.5 28.0 38.0 8.0 40.0 60-61 29.966131342811984 34.0 27.5 38.0 7.0 40.0 62-63 29.389628684458295 34.0 26.5 38.0 2.0 39.5 64-65 28.802453114981358 33.0 26.0 37.0 2.0 39.0 66-67 28.173528476159504 33.0 25.5 36.5 2.0 39.0 68-69 27.646043283733977 32.0 24.5 36.0 2.0 39.0 70-71 26.96834420609838 31.5 22.5 35.5 2.0 38.5 72-73 26.249187122338355 31.0 21.5 35.0 2.0 38.0 74-75 25.522031849005796 31.0 20.0 34.5 2.0 37.0 76-77 24.681136327892325 29.5 20.0 33.5 2.0 36.0 78-79 25.29529431188456 31.0 20.0 34.0 2.0 36.0 80-81 24.937638815450974 31.0 18.5 34.0 2.0 36.0 82-83 24.346849151682367 30.5 16.5 34.0 2.0 35.5 84-85 23.693248543082696 30.0 10.0 34.0 2.0 35.0 86-87 22.980920770314114 29.5 6.0 34.0 2.0 35.0 88-89 22.209808901695354 29.0 2.0 34.0 2.0 35.0 90-91 21.30321172963429 28.5 2.0 33.0 2.0 35.0 92-93 20.316544417741902 26.5 2.0 33.0 2.0 34.0 94-95 19.15354011868832 25.5 2.0 32.0 2.0 34.0 96-97 17.843137561886515 24.0 2.0 32.0 2.0 34.0 98-99 16.390321827677766 10.5 2.0 32.0 2.0 34.0 100-101 13.346125407684212 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09358911886464583 1101 2 0.1594938102139416 1101 3 0.10827963831294696 1101 4 0.21568565760917124 1101 5 0.2559667205181668 1101 6 0.17615884829626793 1101 7 0.21219765549668068 1101 8 0.1946396263658059 1101 9 0.17389733726572842 1101 10-11 0.16413819603314295 1101 12-13 0.28366186264676685 1101 14-15 0.2739356312852266 1101 16-17 0.3359976068657957 1101 18-19 0.2715491182862593 1101 20-21 0.2528223691039244 1101 22-23 0.38462739156757664 1101 24-25 0.23214106321800188 1101 26-27 0.34976406988918285 1101 28-29 0.44914708041267914 1101 30-31 0.5419832336829629 1101 32-33 0.3917157776653326 1101 34-35 0.3496502534016699 1101 36-37 0.3204591710333844 1101 38-39 0.31666798787343 1101 40-41 0.37247905230386635 1101 42-43 0.40018189941820737 1101 44-45 0.4521499022966893 1101 46-47 0.26227439751394854 1101 48-49 0.4208337729970424 1101 50-51 0.31194761585992126 1101 52-53 0.22177342016269463 1101 54-55 0.28536968943132734 1101 56-57 0.374791837557062 1101 58-59 0.32570540459594355 1101 60-61 0.3140851261808173 1101 62-63 0.305013527914479 1101 64-65 0.4121423661584558 1101 66-67 0.30181237206131684 1101 68-69 0.07632164203237934 1101 70-71 0.19039422310544296 1101 72-73 0.2590201426670795 1101 74-75 0.4651466336356016 1101 76-77 0.3698743088697434 1101 78-79 0.41941308699096425 1101 80-81 0.5881379625993084 1101 82-83 0.43974259948373273 1101 84-85 0.23048680016438183 1101 86-87 -0.031282007805199186 1101 88-89 0.22897121699724465 1101 90-91 0.399580980643222 1101 92-93 0.3849370497901816 1101 94-95 0.22984564486020886 1101 96-97 -0.06453358387635433 1101 98-99 0.37309899818925274 1101 100-101 0.7191517819297779 1102 1 0.2025453478208732 1102 2 0.351008961729093 1102 3 0.4087413987747084 1102 4 0.2619386638621748 1102 5 0.3601321846836285 1102 6 0.29718818932560964 1102 7 0.29598793928695954 1102 8 0.32334573507191067 1102 9 0.319053662422057 1102 10-11 0.4209010627960126 1102 12-13 0.402392021376933 1102 14-15 0.5503377476873439 1102 16-17 0.6665411374093253 1102 18-19 0.45876412550126133 1102 20-21 0.5660931723747282 1102 22-23 0.5417173386575271 1102 24-25 0.5446470757240078 1102 26-27 0.5000815302066428 1102 28-29 0.48158575285135186 1102 30-31 0.5855426372423622 1102 32-33 0.7060544020039643 1102 34-35 0.7381687719201864 1102 36-37 0.5413586419328595 1102 38-39 0.8110066122120543 1102 40-41 0.708751298576118 1102 42-43 0.7822213414576495 1102 44-45 0.8087203688671565 1102 46-47 0.9708843061238497 1102 48-49 1.04129072345399 1102 50-51 1.0807932147055226 1102 52-53 1.00352907191834 1102 54-55 0.7060382800999179 1102 56-57 0.9576297403949674 1102 58-59 0.9663980452885852 1102 60-61 1.0615310136267055 1102 62-63 1.0537148266157814 1102 64-65 1.0791601631762546 1102 66-67 1.0578056380545817 1102 68-69 0.9449171206278528 1102 70-71 0.7821162048274246 1102 72-73 0.972464106111044 1102 74-75 1.0715439400329103 1102 76-77 1.0234866224820571 1102 78-79 1.2225395901174672 1102 80-81 1.154443878905223 1102 82-83 0.9869335566746891 1102 84-85 1.1730505327281158 1102 86-87 1.314235067711877 1102 88-89 1.2685383165643458 1102 90-91 1.3385275895898303 1102 92-93 0.9298480647011935 1102 94-95 0.9278879729025356 1102 96-97 1.1641388547960858 1102 98-99 1.1138349289251863 1102 100-101 1.2085601513381476 1103 1 0.18220069782992 1103 2 0.22123014461908852 1103 3 0.29551997925103635 1103 4 0.26797644173275614 1103 5 0.2958925443668221 1103 6 0.18503106456397944 1103 7 0.09357705970566599 1103 8 0.15764342023776123 1103 9 0.28438237443887004 1103 10-11 0.29577279342336027 1103 12-13 0.31335358928382817 1103 14-15 0.43477198440454146 1103 16-17 0.5031525431525594 1103 18-19 0.4617599078539598 1103 20-21 0.4316370818414583 1103 22-23 0.3454671472626387 1103 24-25 0.3386595477107548 1103 26-27 0.4607399286142382 1103 28-29 0.5630933877673456 1103 30-31 0.46920043710595394 1103 32-33 0.4410582299016781 1103 34-35 0.47658249110561 1103 36-37 0.17098657027461428 1103 38-39 0.3639969659098057 1103 40-41 0.50993460615814 1103 42-43 0.6666154896460057 1103 44-45 0.46175707492636775 1103 46-47 0.23680696867030804 1103 48-49 0.4973900260806232 1103 50-51 0.861807259610238 1103 52-53 0.8819998615788514 1103 54-55 0.6695009267779994 1103 56-57 0.5726618251376614 1103 58-59 0.5645738780303304 1103 60-61 0.3958337098586604 1103 62-63 0.8329035906920055 1103 64-65 0.6225125664000402 1103 66-67 0.49358409325425967 1103 68-69 0.2859585871998682 1103 70-71 0.18984981098546783 1103 72-73 0.5601132201267163 1103 74-75 1.0169678623057052 1103 76-77 0.682921485582515 1103 78-79 0.9264036788690149 1103 80-81 0.9579154341452671 1103 82-83 0.9992476948409177 1103 84-85 0.8025133742809878 1103 86-87 0.7141415278112646 1103 88-89 0.9091656742013505 1103 90-91 0.8521788472282275 1103 92-93 0.7515288597903513 1103 94-95 0.7591435233542292 1103 96-97 0.4460901082585913 1103 98-99 0.36022960605941634 1103 100-101 0.56001586604145 1104 1 0.21856422256315255 1104 2 0.2902084856094689 1104 3 0.23542223566830955 1104 4 0.19153927601598042 1104 5 0.08435802976053708 1104 6 0.20831621024085933 1104 7 0.11770948015743699 1104 8 0.12251309253714027 1104 9 0.24394210731050237 1104 10-11 0.3225856193741805 1104 12-13 0.3338657958294249 1104 14-15 0.24013121662974868 1104 16-17 0.4136238419388363 1104 18-19 0.3582739773515442 1104 20-21 0.24028805550577914 1104 22-23 0.22366262741132203 1104 24-25 0.3334781028529079 1104 26-27 0.19578412548370494 1104 28-29 0.1010396323052376 1104 30-31 0.37999187594692074 1104 32-33 0.329549063370969 1104 34-35 0.28914531310949343 1104 36-37 0.4765624452217665 1104 38-39 0.5619285980276558 1104 40-41 0.4570636953991496 1104 42-43 0.5045965548966862 1104 44-45 0.46603511086274807 1104 46-47 0.2872728820868957 1104 48-49 0.26979895281328226 1104 50-51 0.5860013399136506 1104 52-53 0.6030587620863379 1104 54-55 0.24339539107405272 1104 56-57 0.40324809367289305 1104 58-59 0.4315662104567508 1104 60-61 0.48043225423007385 1104 62-63 0.5211424344689206 1104 64-65 -0.0071862563616562625 1104 66-67 0.17112324243601407 1104 68-69 0.16769972809131417 1104 70-71 -0.014822742749821316 1104 72-73 0.06408383390098393 1104 74-75 0.2486483958607728 1104 76-77 0.20107825071198349 1104 78-79 0.4785268652536807 1104 80-81 0.5693566257328655 1104 82-83 0.7577454398269978 1104 84-85 0.8728170073882033 1104 86-87 0.66087197690198 1104 88-89 0.4139217274796714 1104 90-91 0.5722422437300168 1104 92-93 0.49487608632496105 1104 94-95 0.5697070049343331 1104 96-97 0.21330004821259507 1104 98-99 0.18858103388405922 1104 100-101 0.4005821718708056 1105 1 0.2966003755671771 1105 2 0.36508665137725416 1105 3 0.2572782534189457 1105 4 0.24739262824231645 1105 5 0.2115773167327859 1105 6 0.1225297158080707 1105 7 0.23025099167082175 1105 8 0.24167630830181253 1105 9 0.34319352703172257 1105 10-11 0.26945317064342333 1105 12-13 0.2926636736619983 1105 14-15 0.34217539549814546 1105 16-17 0.11990265285137269 1105 18-19 0.22818595646671724 1105 20-21 0.33082297277566397 1105 22-23 0.3619426502586762 1105 24-25 0.0849765449125428 1105 26-27 0.15917069607433376 1105 28-29 0.24348499454925587 1105 30-31 0.23524164069976194 1105 32-33 0.3072976106968355 1105 34-35 0.4335490851192887 1105 36-37 0.3542860947931956 1105 38-39 0.4711520260823008 1105 40-41 0.34358714505625443 1105 42-43 0.7014691406383307 1105 44-45 0.5930557650146326 1105 46-47 0.5224285113593368 1105 48-49 0.2588535521242008 1105 50-51 0.50303369234868 1105 52-53 0.6166564143745497 1105 54-55 0.7920686702913855 1105 56-57 0.6285434798993101 1105 58-59 0.42468271364036525 1105 60-61 0.5190947139085225 1105 62-63 0.7113653975213836 1105 64-65 0.13625638909798 1105 66-67 0.2580319665359454 1105 68-69 0.21706593378337757 1105 70-71 -0.10340748848150128 1105 72-73 0.1391620055270657 1105 74-75 0.5607664624769093 1105 76-77 0.3355458049103639 1105 78-79 0.45699076534045346 1105 80-81 0.5525648295429555 1105 82-83 0.5040771401605539 1105 84-85 0.6683121614796761 1105 86-87 0.26721147609097073 1105 88-89 0.4262067555079554 1105 90-91 0.3908854442631231 1105 92-93 0.43751330773556774 1105 94-95 0.25255080447811906 1105 96-97 0.12807054949958996 1105 98-99 0.14480721167599242 1105 100-101 0.032824946945225975 1106 1 0.18541549317016148 1106 2 0.1517067621408401 1106 3 0.0812330787276494 1106 4 0.15524207082470554 1106 5 0.27767749991667046 1106 6 0.3232033898580866 1106 7 0.22934707121581965 1106 8 0.19217133140644904 1106 9 0.1576825186671229 1106 10-11 0.14913009240763841 1106 12-13 0.33580254410806987 1106 14-15 0.407764956950075 1106 16-17 0.2579571707204735 1106 18-19 0.08516320913514619 1106 20-21 0.2753608534421659 1106 22-23 0.13027003744860366 1106 24-25 0.06169659354825541 1106 26-27 0.308821526801232 1106 28-29 0.4071885886329696 1106 30-31 0.4583446742994681 1106 32-33 0.4434509625352021 1106 34-35 0.15714374212280546 1106 36-37 0.23602916806941465 1106 38-39 0.4579315228199192 1106 40-41 0.4417161763681676 1106 42-43 0.22335921431184858 1106 44-45 0.05863879370811276 1106 46-47 0.23047287012242634 1106 48-49 0.25399431307057085 1106 50-51 0.3214846686627624 1106 52-53 0.2667252083206826 1106 54-55 0.18210301393580863 1106 56-57 0.40955008465142484 1106 58-59 0.4906949045675617 1106 60-61 0.20043508928882403 1106 62-63 0.49013186607215076 1106 64-65 0.1994578273714147 1106 66-67 0.39352271001718364 1106 68-69 0.2889929375403817 1106 70-71 0.1598180143409138 1106 72-73 -0.0023025455924390315 1106 74-75 0.41230752686324124 1106 76-77 0.36041261663093493 1106 78-79 0.6640848743921914 1106 80-81 0.7522969197856817 1106 82-83 0.8722450747156465 1106 84-85 0.7239099355422276 1106 86-87 0.7317689283685027 1106 88-89 0.599091541646775 1106 90-91 0.5356083753118561 1106 92-93 0.5654293844541485 1106 94-95 0.45873932655484495 1106 96-97 0.5202063748802921 1106 98-99 0.45312907935163693 1106 100-101 0.48864683461910907 1107 1 0.044725128824183 1107 2 0.06656451728465029 1107 3 0.1583348118975323 1107 4 0.2083065443477068 1107 5 0.09855506898886546 1107 6 0.058016641918769096 1107 7 0.22074814640011198 1107 8 0.1767096260828609 1107 9 0.2620873607498737 1107 10-11 0.3988367219081326 1107 12-13 0.2931262544641058 1107 14-15 0.22182064387612144 1107 16-17 0.17422299803236285 1107 18-19 0.20636789428150593 1107 20-21 0.18260620182893206 1107 22-23 0.29505236963961323 1107 24-25 0.26342307097059603 1107 26-27 0.40866823761687954 1107 28-29 0.3573127709313155 1107 30-31 0.3542176223879423 1107 32-33 0.3410650123086896 1107 34-35 0.3278538580758621 1107 36-37 0.6328703981504944 1107 38-39 0.5177445801264966 1107 40-41 0.5212425981262356 1107 42-43 0.5626440559980068 1107 44-45 0.3067222362807911 1107 46-47 0.0147600382348827 1107 48-49 0.386217300145276 1107 50-51 0.25376494885372836 1107 52-53 0.4706812661293647 1107 54-55 0.7215649756266203 1107 56-57 0.7567401724465732 1107 58-59 0.8016242569853844 1107 60-61 0.6629010161731763 1107 62-63 0.7018092158866409 1107 64-65 0.44255715833795506 1107 66-67 0.6724953927443345 1107 68-69 0.5178022746894797 1107 70-71 0.6357228596105529 1107 72-73 0.3897716351832763 1107 74-75 0.48565988944297445 1107 76-77 0.5555603004380849 1107 78-79 0.9575196427445789 1107 80-81 0.8968002153792085 1107 82-83 0.8217998668351179 1107 84-85 0.8582504308691874 1107 86-87 0.5152689358045706 1107 88-89 0.5843824724084996 1107 90-91 0.6081583509852955 1107 92-93 0.6036805067689315 1107 94-95 0.9395321457231809 1107 96-97 0.7181716194170846 1107 98-99 0.40819364210743103 1107 100-101 0.4568310960796804 1108 1 0.0651412902918942 1108 2 0.13130309607694457 1108 3 0.08027234128628535 1108 4 0.17089751263364406 1108 5 0.25427399540257767 1108 6 0.13429843697926458 1108 7 0.16730375651158624 1108 8 0.2925708135537022 1108 9 0.15556785403931173 1108 10-11 0.2909499770298041 1108 12-13 0.15391160734997555 1108 14-15 0.19942135614634537 1108 16-17 0.39445875383412243 1108 18-19 0.4339406791162901 1108 20-21 0.25145929906502573 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2316 72-73 -1.0970754452466913 2316 74-75 -1.2963427187628405 2316 76-77 -1.228201689206255 2316 78-79 -1.8304710356204303 2316 80-81 -1.8444935473049284 2316 82-83 -1.965581201294615 2316 84-85 -1.6656979914749535 2316 86-87 -1.8344544246139804 2316 88-89 -2.445133466198346 2316 90-91 -1.8601264836096973 2316 92-93 -1.5246501643424857 2316 94-95 -1.3446503978176523 2316 96-97 -0.89109136952505 2316 98-99 -0.7007105256202681 2316 100-101 -0.6060089159581921 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 18.0 4 43.0 5 105.0 6 225.0 7 544.0 8 1265.0 9 2212.0 10 3377.0 11 5029.0 12 7678.0 13 10063.0 14 13148.0 15 17286.0 16 21882.0 17 26691.0 18 25010.0 19 20877.0 20 21220.0 21 22816.0 22 24108.0 23 26943.0 24 30248.0 25 34713.0 26 40345.0 27 47241.0 28 55834.0 29 64746.0 30 74929.0 31 85104.0 32 97127.0 33 112151.0 34 126696.0 35 137656.0 36 133017.0 37 91584.0 38 22804.0 39 458.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.87055927714154 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21.68321961189535 27.54168837194738 30.034699983205094 20.740392032952172 20-21 22.53790352051108 26.478859106612358 30.321464268443997 20.661773104432566 22-23 22.318975897612532 26.605532988132413 30.22996473029636 20.845526383958692 24-25 22.18208138767719 26.88941271254536 30.20555096498139 20.722954934796054 26-27 22.048119973269053 26.939150089708086 30.089323776806566 20.923406160216295 28-29 21.750281634311637 26.982879907428952 30.319792825316988 20.947045632942427 30-31 21.979873473528468 26.75989682317429 30.254301910707 21.005927792590242 32-33 21.915823334329676 26.97193873920036 30.05006618557581 21.062171740894144 34-35 22.230611738031715 26.87079283770984 29.98570303154086 20.912892392717584 36-37 22.405691934101785 26.624751970711902 29.902475026261925 21.067081068924384 38-39 22.064628858256718 26.94095646155133 30.030479938933365 20.96393474125859 40-41 22.311032702641732 26.84259705360962 29.847093995987695 20.99927624776096 42-43 22.256088665400412 27.001735704632747 29.512351684074716 21.22982394589213 44-45 22.383900289853422 27.1069169857806 29.17866086722607 21.33052185713991 46-47 22.574681102184293 27.381356918256337 28.65620095196989 21.387761027589477 48-49 22.700970282345473 27.66977094390809 28.303009341298274 21.326249432448165 50-51 22.557484455053274 27.852374069384872 28.058050599273088 21.53209087628877 52-53 22.87390415409129 27.940539128788718 27.791484870761522 21.39407184635847 54-55 22.702053934186715 27.846745950808344 27.854004926139925 21.59719518886502 56-57 22.64022440316849 28.112987265767806 27.746869639138044 21.49991869192566 58-59 22.8765860844441 27.869505191468768 27.76549790419801 21.488410819889125 60-61 22.63612568425787 27.86783397334988 28.15075307506839 21.345287267323858 62-63 22.56589854273633 27.744445634525118 28.26336429954876 21.42629152318979 64-65 22.543415744314128 27.80500614506335 28.063511560333705 21.588066550288822 66-67 22.468174714554518 28.106879442516647 27.746069520314954 21.678876322613885 68-69 22.494206293319365 27.93326304620341 27.845160987298144 21.72736967317908 70-71 22.59118563204935 27.880318791217938 27.68297794664467 21.845517630088036 72-73 22.689232012969036 27.930789578371083 27.542230853697674 21.8377475549622 74-75 22.56629516372484 28.23153118468424 27.26589870572939 21.936274945861527 76-77 22.691117874911132 28.071019425801296 27.447226110576985 21.79063658871059 78-79 22.66344856101502 28.03744303643427 27.378378984247153 21.920729418303566 80-81 22.530938328300152 28.202013344250126 27.36542685623743 21.901621471212295 82-83 22.604386406921233 28.2389179740767 27.334299834598752 21.822395784403316 84-85 22.532639677179446 28.17758688790597 27.23197033694751 22.05780309796707 86-87 22.646875779493914 27.99488456284469 27.291867785733782 22.066371871927615 88-89 22.640144549641615 28.008663584762793 27.113412259676966 22.23777960591862 90-91 22.579715425573553 28.156059055527166 27.14985774125276 22.114367777646518 92-93 22.58928593667549 28.11266515109517 26.987159392267458 22.310889519961886 94-95 22.51911303287164 28.09699450538111 26.931674154368828 22.452218307378416 96-97 22.551656579508737 28.08614903753604 26.85359310745074 22.508601275504486 98-99 22.389370422940654 28.147271493520194 26.67570944086264 22.78764864267651 100-101 22.646973007208988 28.27397006810467 26.385044015400055 22.694012909286286 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 580.0 1 404.5 2 269.5 3 319.5 4 302.5 5 332.5 6 532.5 7 1045.0 8 1684.0 9 2025.0 10 1900.0 11 1693.5 12 1791.5 13 1924.0 14 2157.5 15 2411.0 16 2537.5 17 2766.0 18 2965.0 19 3180.0 20 3469.0 21 3875.5 22 4535.5 23 5314.5 24 6330.5 25 7768.0 26 9647.5 27 11648.5 28 13617.5 29 15689.0 30 18085.5 31 20965.5 32 23723.5 33 26281.0 34 29293.5 35 31641.0 36 34196.0 37 37215.5 38 39211.5 39 40489.0 40 41712.0 41 43806.5 42 45716.0 43 47066.0 44 48859.5 45 50434.0 46 50695.0 47 51394.0 48 53996.5 49 54105.0 50 51501.5 51 49059.5 52 46694.0 53 45175.5 54 43357.5 55 40333.0 56 36758.0 57 33125.0 58 29700.0 59 26420.0 60 22848.5 61 18862.0 62 15110.0 63 11814.0 64 9002.0 65 6761.5 66 5032.0 67 3880.5 68 2759.0 69 1778.0 70 1209.5 71 826.5 72 585.0 73 418.0 74 292.5 75 207.5 76 141.0 77 90.0 78 55.5 79 28.5 80 21.0 81 17.5 82 8.0 83 4.5 84 1.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023199660117862814 2 0.0028465840635414495 3 0.0 4 7.116460158853624E-5 5 7.116460158853624E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.009963044222395074 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.558230079426812E-4 20-21 9.607221214452392E-4 22-23 0.001494456633359261 24-25 0.009963044222395074 26-27 0.005693168127082899 28-29 1.4232920317707248E-4 30-31 0.0023128495516274274 32-33 0.00377172388419242 34-35 0.0 36-37 0.00832625838585874 38-39 0.012133564570845428 40-41 0.0011386336254165799 42-43 0.0 44-45 0.0 46-47 1.0674690238280437E-4 48-49 0.0024907610555987684 50-51 0.005266180517551681 52-53 0.0 54-55 0.0025619256571873046 56-57 0.0030956601691013263 58-59 2.1349380476560874E-4 60-61 0.001494456633359261 62-63 0.004234293794517906 64-65 0.0 66-67 0.001494456633359261 68-69 3.202407071484131E-4 70-71 7.116460158853624E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0013165451293879202 80-81 0.0013165451293879202 82-83 0.00889557519856703 84-85 0.005550838923905827 86-87 0.0024551787548045003 88-89 7.116460158853624E-5 90-91 4.981522111197536E-4 92-93 1.779115039713406E-4 94-95 0.0 96-97 0.0016723681373306016 98-99 0.0013877097309764567 100-101 2.1349380476560874E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1405193.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.58638383972328 #Duplication Level Percentage of deduplicated Percentage of total 1 80.73224023849383 50.52738975807127 2 11.454113954494572 14.337431449998558 3 3.4254171213283695 6.43153412299852 4 1.4609107882802594 3.657324934036041 5 0.7810571090111139 2.444177001265708 6 0.47490611246027226 1.7833593745361636 7 0.3088815346182071 1.353224480063252 8 0.2212935839452745 1.1079972148853579 9 0.16490548097636393 0.9288753956694809 >10 0.8900928257151353 10.152312471409514 >50 0.05757021211856973 2.4873805606476243 >100 0.027243210733562836 3.0114983115123084 >500 6.839139122455538E-4 0.2506156554501189 >1k 4.5594260816370254E-4 0.5836997702513159 >5k 2.2797130408185127E-4 0.9431794992046746 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7152 0.5089692305612112 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6069 0.4318979670408264 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4080 0.29035157448122784 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1605 0.11421918554960066 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1464 0.10418497672561705 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 7.116460158853624E-5 16-17 0.0 0.0 0.0 7.116460158853624E-5 7.116460158853624E-5 18-19 0.0 0.0 0.0 1.4232920317707248E-4 1.4232920317707248E-4 20-21 0.0 0.0 0.0 1.779115039713406E-4 1.4232920317707248E-4 22-23 0.0 0.0 0.0 4.981522111197537E-4 1.4232920317707248E-4 24-25 0.0 0.0 0.0 8.539752190624348E-4 1.4232920317707248E-4 26-27 0.0 0.0 0.0 0.0013521274301821885 1.4232920317707248E-4 28-29 0.0 0.0 0.0 0.0029177486651299858 1.4232920317707248E-4 30-31 0.0 0.0 0.0 0.006653890248528138 1.4232920317707248E-4 32-33 0.0 0.0 0.0 0.010247702628749219 1.4232920317707248E-4 34-35 0.0 0.0 0.0 0.015335971642329558 1.4232920317707248E-4 36-37 0.0 0.0 0.0 0.02255917870356599 1.4232920317707248E-4 38-39 0.0 0.0 0.0 0.03273571673072667 1.4232920317707248E-4 40-41 0.0 0.0 0.0 0.04849867598258745 1.4232920317707248E-4 42-43 0.0 0.0 0.0 0.06404814142968261 1.4232920317707248E-4 44-45 0.0 0.0 0.0 0.0826932670458791 1.4232920317707248E-4 46-47 0.0 0.0 0.0 0.11311613422497835 1.4232920317707248E-4 48-49 0.0 0.0 0.0 0.1388421376992342 1.4232920317707248E-4 50-51 0.0 0.0 0.0 0.1647460526774614 1.4232920317707248E-4 52-53 0.0 0.0 0.0 0.19054322075330576 1.779115039713406E-4 54-55 0.0 0.0 0.0 0.21847532687680626 2.134938047656087E-4 56-57 0.0 0.0 0.0 0.26273970906487576 2.134938047656087E-4 58-59 0.0 0.0 0.0 0.3059366222291173 2.134938047656087E-4 60-61 0.0 0.0 0.0 0.34696301504490845 2.134938047656087E-4 62-63 0.0 0.0 0.0 0.39072924502185824 2.134938047656087E-4 64-65 0.0 0.0 0.0 0.43655924844487554 2.134938047656087E-4 66-67 0.0 0.0 0.0 0.4906443456521631 2.4907610555987685E-4 68-69 0.0 0.0 0.0 0.5549771454881999 2.8465840635414496E-4 70-71 0.0 0.0 0.0 0.6312655983911106 2.8465840635414496E-4 72-73 0.0 0.0 0.0 0.7091552548297636 3.558230079426812E-4 74-75 0.0 0.0 0.0 0.794019042224093 3.558230079426812E-4 76-77 0.0 0.0 0.0 0.8919415340099189 3.558230079426812E-4 78-79 0.0 0.0 0.0 0.9991510063030488 3.558230079426812E-4 80-81 0.0 0.0 0.0 1.1248276927084038 3.558230079426812E-4 82-83 0.0 0.0 0.0 1.251607430438381 3.558230079426812E-4 84-85 0.0 0.0 0.0 1.3857171221319775 3.558230079426812E-4 86-87 0.0 0.0 0.0 1.5210721943533736 3.558230079426812E-4 88-89 0.0 0.0 0.0 1.6655007532773078 3.558230079426812E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5545 0.0 38.29517 1 GTATCAA 8400 0.0 31.72646 1 TATCAAC 10580 0.0 25.093197 2 ATCAACG 10685 0.0 24.757713 3 TCAACGC 10735 0.0 24.598158 4 CAACGCA 10955 0.0 24.110178 5 AACGCAG 11240 0.0 23.583372 6 GAGTACT 7420 0.0 21.218245 12-13 AGTACTT 7585 0.0 21.03844 12-13 ACGCAGA 12825 0.0 20.372454 7 CGCAGAG 12910 0.0 19.907148 8 GTACTTT 7850 0.0 19.78407 14-15 AGAGTAC 11050 0.0 19.474842 10-11 GCAGAGT 13465 0.0 18.769094 9 TACTTTT 8665 0.0 18.306927 14-15 GTACATG 5940 0.0 17.354467 1 TAGGACG 165 0.0014654727 17.270191 4 CAGAGTA 13190 0.0 17.125519 10-11 ACTTTTT 9330 0.0 16.824226 16-17 TATACGC 200 2.7639748E-4 16.622559 3 >>END_MODULE