FastQCFastQC Report
Thu 26 May 2016
SRR937060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1404198
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82730.5891619273065479No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58350.41553968884729936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40360.2874238533312254No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14080.10027075953676048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34000.048.368041
GTATCAA62400.042.6544881
ATCAACG81150.032.392493
TATCAAC82150.032.2296372
CAACGCA82500.031.863575
TCAACGC82650.031.8057444
AACGCAG85200.030.8527096
ACGCAGA96350.027.1294697
CGCAGAG97200.026.5969628
GTACATG56900.024.7252521
GCAGAGT101700.024.530529
TGTACCG1201.7022951E-423.7680345
TACATGG59050.023.5860632
AGAGTAC90300.022.39134810-11
ACATGGG60250.022.2484533
GAGTACT58950.022.08692212-13
AGTACTT61300.021.5890312-13
GTGGTAT17200.021.553981
GTGTAGC7600.021.2631021
CATGGGA32150.020.8478554
Produced by FastQC (version 0.11.5)