FastQCFastQC Report
Thu 26 May 2016
SRR937060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1404198
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72330.5150982981032589No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61950.44117709895613016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40930.2914831099317903No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15800.1125197443665352No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15210.10831805770981015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53550.038.854761
GTATCAA82600.033.758851
TATCAAC101700.027.1345672
ATCAACG104800.026.241283
TCAACGC104800.026.241284
CAACGCA106650.025.7860855
AACGCAG110100.024.9816346
ACGGTAT1201.7092926E-423.753668
GAGTACT71750.021.84888812-13
ACGCAGA125400.021.7442257
CGCAGAG126100.021.5873878
GTACTTT75150.020.57409114-15
AGTACTT74500.020.27720812-13
GCAGAGT130450.020.2120069
AGAGTAC105950.020.04320110-11
TACTTTT83400.017.85542314-15
ACTTTTT87950.017.7958316-17
CAGAGTA127200.017.68452510-11
GAGTACA55300.017.4381541
AGTACAT50000.017.3839652
Produced by FastQC (version 0.11.5)