##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937060_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1404198 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.124602086030603 31.0 31.0 33.0 30.0 34.0 2 31.27953109176911 31.0 31.0 34.0 30.0 34.0 3 31.345107313925816 31.0 31.0 34.0 28.0 34.0 4 35.1781123459797 37.0 35.0 37.0 32.0 37.0 5 34.88861898393247 35.0 35.0 37.0 32.0 37.0 6 35.0721899618145 36.0 35.0 37.0 32.0 37.0 7 35.03256307158962 36.0 35.0 37.0 32.0 37.0 8 34.97981481244098 36.0 35.0 37.0 32.0 37.0 9 36.46843749955491 38.0 35.0 39.0 32.0 39.0 10-11 36.29385029746517 38.0 35.0 39.0 32.0 39.0 12-13 36.12298835349431 38.0 35.0 39.0 32.0 39.0 14-15 36.961664238234206 38.0 36.0 40.0 31.0 41.0 16-17 36.924894138860765 38.0 36.0 40.0 31.0 41.0 18-19 37.078288104669 38.5 36.0 40.0 31.0 41.0 20-21 37.067391493222466 39.0 36.0 40.0 31.0 41.0 22-23 36.91172220726706 39.0 36.0 40.0 31.0 41.0 24-25 36.75118216946613 38.5 35.5 40.0 30.5 41.0 26-27 36.41108127201434 38.0 35.0 40.0 30.0 41.0 28-29 36.14844523350696 38.0 35.0 40.0 30.0 41.0 30-31 36.05303133888526 38.0 35.0 40.0 30.0 41.0 32-33 35.85134503823535 38.0 34.0 40.0 29.0 41.0 34-35 35.6133647106747 38.0 34.0 40.0 28.5 41.0 36-37 35.359117802475154 38.0 34.0 40.0 27.0 41.0 38-39 35.08723093182016 38.0 34.0 40.0 27.0 41.0 40-41 34.58832657502717 37.5 33.0 40.0 25.0 41.0 42-43 34.245095421016124 37.0 33.0 40.0 24.0 41.0 44-45 33.88114745926144 37.0 33.0 40.0 24.0 41.0 46-47 33.34190477411305 37.0 32.0 39.5 22.5 40.0 48-49 32.813043103607896 37.0 31.0 39.0 19.0 40.0 50-51 32.37286087859405 36.0 31.0 39.0 17.0 40.0 52-53 31.950698548210436 36.0 30.0 39.0 14.5 40.0 54-55 31.377874060495742 35.0 29.5 39.0 9.0 40.0 56-57 31.02092511170077 35.0 29.0 38.0 8.5 40.0 58-59 30.531994419590397 34.5 28.0 38.0 8.0 40.0 60-61 29.949158523228206 34.0 27.5 38.0 7.0 40.0 62-63 29.404162375961224 34.0 26.5 38.0 3.5 39.5 64-65 28.913711955151623 33.5 26.0 37.0 2.0 39.0 66-67 28.352834856622785 33.0 26.0 36.5 2.0 39.0 68-69 27.711062115171792 32.0 24.5 36.0 2.0 39.0 70-71 27.099027701221623 32.0 23.0 35.5 2.0 38.5 72-73 26.528374915788227 31.0 22.0 35.0 2.0 38.0 74-75 25.881468639038083 31.0 20.5 35.0 2.0 37.0 76-77 24.983598110807733 29.5 20.5 34.0 2.0 36.0 78-79 25.581127803913695 31.0 21.0 34.0 2.0 36.0 80-81 25.30574320715455 31.0 20.0 34.0 2.0 36.0 82-83 24.74849878720807 31.0 18.5 34.0 2.0 35.5 84-85 24.037865742580465 30.0 15.0 34.0 2.0 35.0 86-87 23.349412262373257 30.0 7.5 34.0 2.0 35.0 88-89 22.633191686642483 29.0 4.0 34.0 2.0 35.0 90-91 21.755215432581444 29.0 2.0 33.5 2.0 35.0 92-93 20.787554176832614 27.5 2.0 33.0 2.0 34.5 94-95 19.696992518149152 26.5 2.0 32.5 2.0 34.0 96-97 18.360990757713658 25.0 2.0 32.0 2.0 34.0 98-99 16.898196693058956 20.5 2.0 32.0 2.0 34.0 100-101 13.993196116217229 2.0 2.0 28.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19967496665786655 1101 2 0.299468049713596 1101 3 0.1718699036130431 1101 4 0.18284007205722475 1101 5 0.0811236683959109 1101 6 0.16472944467357564 1101 7 0.07244658485956279 1101 8 0.20192281329197215 1101 9 0.298150840493399 1101 10-11 0.32268528099300653 1101 12-13 0.3776004225218159 1101 14-15 0.47399680975274094 1101 16-17 0.5261332913256567 1101 18-19 0.3029985871941179 1101 20-21 0.2740259238297398 1101 22-23 0.3519031778706889 1101 24-25 0.30663909206758433 1101 26-27 0.34998882381701435 1101 28-29 0.4412433565165088 1101 30-31 0.47334495751486827 1101 32-33 0.3802717816650798 1101 34-35 0.06778849009069177 1101 36-37 0.27782067584610814 1101 38-39 0.43221792174576734 1101 40-41 0.5379391039435504 1101 42-43 0.7116027144337949 1101 44-45 0.6296826586728272 1101 46-47 0.5819496074395332 1101 48-49 0.650607930981451 1101 50-51 0.5677578025052412 1101 52-53 0.9489968202423853 1101 54-55 0.847690309437926 1101 56-57 1.0019733343597537 1101 58-59 0.8722023037658673 1101 60-61 0.884778538556688 1101 62-63 0.9025187323574926 1101 64-65 0.9053920530718358 1101 66-67 1.0014665583139752 1101 68-69 0.9838267743498292 1101 70-71 0.6477820084145485 1101 72-73 0.6313880672797971 1101 74-75 0.6763372027400045 1101 76-77 0.6745973071092131 1101 78-79 0.8597532079203845 1101 80-81 0.9874891232451262 1101 82-83 1.291251649007986 1101 84-85 1.0983959113813384 1101 86-87 0.8042847126583084 1101 88-89 0.9428136619785441 1101 90-91 1.1424685017015612 1101 92-93 0.646915980917047 1101 94-95 0.7382641872182525 1101 96-97 0.7957543234961868 1101 98-99 0.7061957328434474 1101 100-101 0.9221568173124943 1102 1 -0.021709866125927846 1102 2 0.0456236009590576 1102 3 0.148564924532387 1102 4 0.12496968885920268 1102 5 0.031188781582024205 1102 6 0.09342634971915942 1102 7 0.08594025364125457 1102 8 0.2927127609243456 1102 9 0.2624411067647685 1102 10-11 0.2679666530802294 1102 12-13 0.316978660863775 1102 14-15 0.4592784589173533 1102 16-17 0.3116878617171679 1102 18-19 0.3271154030207768 1102 20-21 0.23183119384165707 1102 22-23 0.15107333109448717 1102 24-25 0.2328894037796445 1102 26-27 0.4783463090626441 1102 28-29 0.37998154609285706 1102 30-31 0.26222861271976683 1102 32-33 0.02474131700629556 1102 34-35 0.3513342494212708 1102 36-37 0.4074887371356226 1102 38-39 0.44208464752227883 1102 40-41 0.4645731678375995 1102 42-43 0.5463662288833717 1102 44-45 0.5958487388590257 1102 46-47 0.4091835438779796 1102 48-49 0.592517271260192 1102 50-51 0.45268894877439436 1102 52-53 0.7280539258919987 1102 54-55 1.0629087849581431 1102 56-57 0.7642658726656997 1102 58-59 0.6763487668730122 1102 60-61 0.8825716171635456 1102 62-63 0.9710108772135513 1102 64-65 0.9202614110835299 1102 66-67 0.9139987685602975 1102 68-69 0.7783558813294071 1102 70-71 0.45666788023854465 1102 72-73 0.6354264702057364 1102 74-75 0.7136844638300239 1102 76-77 0.8319537117530302 1102 78-79 0.6407041375150193 1102 80-81 0.8630093360405553 1102 82-83 1.011927163679232 1102 84-85 0.6294881504437093 1102 86-87 0.6032332039719321 1102 88-89 0.6436712164935194 1102 90-91 0.7086359672746951 1102 92-93 0.6250407211569957 1102 94-95 0.6060165354491147 1102 96-97 0.946650668845443 1102 98-99 1.3291335843853815 1102 100-101 1.046758518567657 1103 1 0.11171032302674533 1103 2 0.20405315691577997 1103 3 0.15861181532113022 1103 4 0.21767720614521124 1103 5 -0.10683987516150495 1103 6 0.03265679580610481 1103 7 0.10720176345652987 1103 8 0.016782608898274987 1103 9 0.16789826357074844 1103 10-11 0.09957933289447851 1103 12-13 0.24617186680858083 1103 14-15 0.27412001800769303 1103 16-17 0.25607317229768256 1103 18-19 0.2515701414882514 1103 20-21 0.10984872395734868 1103 22-23 0.16115314761865562 1103 24-25 0.10840883564040382 1103 26-27 0.13067097952144735 1103 28-29 0.1809307155695592 1103 30-31 0.2957067057519964 1103 32-33 0.1007271021715539 1103 34-35 0.24393504190966553 1103 36-37 0.1298646127898877 1103 38-39 0.19694488345220407 1103 40-41 0.4309863631099091 1103 42-43 0.3875347518802954 1103 44-45 0.3588016096425477 1103 46-47 0.2788592253839326 1103 48-49 0.2206542990881175 1103 50-51 0.17790268221217787 1103 52-53 0.00962573237950437 1103 54-55 0.2712580134740996 1103 56-57 0.12230599664812658 1103 58-59 0.17508339679909923 1103 60-61 -0.02727771921258082 1103 62-63 0.45972586523539505 1103 64-65 0.1466877198815162 1103 66-67 0.4806245619002141 1103 68-69 0.3669158913206658 1103 70-71 0.21653964017587768 1103 72-73 0.4293037026273687 1103 74-75 0.2767849327379146 1103 76-77 0.6605562413578063 1103 78-79 0.49803522281538193 1103 80-81 0.4678522575751245 1103 82-83 0.6209093610746947 1103 84-85 0.10950308056167302 1103 86-87 0.19459388837307756 1103 88-89 0.6627956757734665 1103 90-91 0.3464911962220931 1103 92-93 0.5965293050097635 1103 94-95 0.688061223619691 1103 96-97 0.7333171652065822 1103 98-99 0.642360930911714 1103 100-101 0.8293953022909886 1104 1 0.12212918665352035 1104 2 0.16349200057658209 1104 3 0.07525036089838366 1104 4 0.13646701506240788 1104 5 -0.08586154246089706 1104 6 0.13215272527840938 1104 7 0.1449402654460954 1104 8 0.1138038825024168 1104 9 0.08571789490326864 1104 10-11 0.2361951003893168 1104 12-13 0.242089036139717 1104 14-15 0.3127408191775345 1104 16-17 0.27408648763351806 1104 18-19 0.2296690855413388 1104 20-21 0.2705519621007966 1104 22-23 0.21314201166725155 1104 24-25 0.11195912317854351 1104 26-27 0.23919377869468406 1104 28-29 0.4227000242125811 1104 30-31 0.39695668817631713 1104 32-33 0.4714017271065245 1104 34-35 0.32735625364740883 1104 36-37 0.5426804477753322 1104 38-39 0.49904271672225775 1104 40-41 0.4662326335504332 1104 42-43 0.5007115913510631 1104 44-45 0.4766676407725967 1104 46-47 0.41461554208364504 1104 48-49 0.642667487879045 1104 50-51 0.5934743839599079 1104 52-53 0.662423429262283 1104 54-55 0.9916482638924045 1104 56-57 0.8415380096963112 1104 58-59 0.3805536517167738 1104 60-61 0.44986335023244806 1104 62-63 0.7179020719816478 1104 64-65 0.8810365760562 1104 66-67 0.6607137760563724 1104 68-69 0.3979896951198256 1104 70-71 0.6028478332552751 1104 72-73 0.7167275999291469 1104 74-75 0.6696875212841924 1104 76-77 1.115852497034755 1104 78-79 1.2862306925078784 1104 80-81 0.9043056257786048 1104 82-83 1.0692157684649146 1104 84-85 1.0260432023259902 1104 86-87 0.8606770424344674 1104 88-89 0.7278415131492046 1104 90-91 0.7586707915596165 1104 92-93 0.7507699652929425 1104 94-95 0.9619694223233068 1104 96-97 1.2082155777710817 1104 98-99 1.119776180978505 1104 100-101 1.2350947440873625 1105 1 0.03755365516290965 1105 2 0.14871708231017067 1105 3 0.25802813444235895 1105 4 0.0999307665008402 1105 5 0.018286865925084328 1105 6 0.14120946756928987 1105 7 0.1549242108605 1105 8 0.018198444265578928 1105 9 0.1519557618788312 1105 10-11 0.06201999363393895 1105 12-13 -0.017773928319186894 1105 14-15 0.09069726349326857 1105 16-17 0.0983764151592581 1105 18-19 0.16100493121410153 1105 20-21 0.09606662334141447 1105 22-23 0.052325615667690784 1105 24-25 -0.12783624436223562 1105 26-27 -0.053065726407474756 1105 28-29 -0.007715604113599284 1105 30-31 0.2829996138705084 1105 32-33 0.27791602762702183 1105 34-35 0.33069863038655 1105 36-37 0.22093556580955465 1105 38-39 0.3989857508865242 1105 40-41 0.4137608751414632 1105 42-43 0.47766995866066964 1105 44-45 0.5805099451136826 1105 46-47 0.4416549444059683 1105 48-49 0.5403579265096354 1105 50-51 0.39268759495323025 1105 52-53 0.6196974647805149 1105 54-55 0.8219523103084434 1105 56-57 0.5658735352934663 1105 58-59 0.633141564544438 1105 60-61 0.5430028092030739 1105 62-63 0.6627931827709865 1105 64-65 0.6265532949256745 1105 66-67 0.6011171602207597 1105 68-69 0.36688901610230573 1105 70-71 0.3064546534058117 1105 72-73 0.38856056619165713 1105 74-75 0.438929843915318 1105 76-77 0.3952651583109379 1105 78-79 0.7487119896777514 1105 80-81 0.5842744819147896 1105 82-83 0.7425878284054228 1105 84-85 0.33876182539124855 1105 86-87 0.4981326827507857 1105 88-89 0.8419687863845375 1105 90-91 0.6871440563561286 1105 92-93 0.8867309668755361 1105 94-95 0.9901802124273829 1105 96-97 0.8808312685882171 1105 98-99 0.9578988994872581 1105 100-101 0.8771721109321042 1106 1 0.11050665684689776 1106 2 0.2042506881575754 1106 3 0.16745611737315969 1106 4 0.3378475672832977 1106 5 0.11553888525985911 1106 6 0.07584477163337766 1106 7 0.09959298746205292 1106 8 0.31886140219904036 1106 9 0.3019805589092641 1106 10-11 0.27561152921249743 1106 12-13 0.29861131392500084 1106 14-15 0.3900816893004162 1106 16-17 0.41247363057016884 1106 18-19 0.23891284927200473 1106 20-21 0.3876044122199147 1106 22-23 0.3301576921116194 1106 24-25 0.3538104994906561 1106 26-27 0.48349746043228237 1106 28-29 0.2969083168011366 1106 30-31 0.3244462270382158 1106 32-33 0.11760391659158387 1106 34-35 0.15387162800907106 1106 36-37 0.19397242151846683 1106 38-39 0.4904912829037045 1106 40-41 0.6605351890097069 1106 42-43 0.7553207197706371 1106 44-45 0.48869496825987824 1106 46-47 0.33283846039004317 1106 48-49 0.2181529800891795 1106 50-51 0.3542604864803849 1106 52-53 0.3947842543870337 1106 54-55 0.7268125282736513 1106 56-57 0.6701263986942969 1106 58-59 0.6042053640573428 1106 60-61 0.39182622809882517 1106 62-63 0.38334308282307106 1106 64-65 0.2551189904789233 1106 66-67 0.2901910636601528 1106 68-69 0.30794771806409926 1106 70-71 0.20559290479382497 1106 72-73 0.0892680068203795 1106 74-75 0.12297181513418565 1106 76-77 0.33367803784710404 1106 78-79 0.3626129010204231 1106 80-81 0.5622071970031257 1106 82-83 0.7504462076604597 1106 84-85 0.5577158051419495 1106 86-87 0.5877470094941408 1106 88-89 0.744587946822012 1106 90-91 0.6216557206305815 1106 92-93 0.7215920689688495 1106 94-95 0.9665152804016373 1106 96-97 1.135115145545214 1106 98-99 1.1270436575055385 1106 100-101 1.0569844271128925 1107 1 0.23472238087235553 1107 2 0.33232687298330177 1107 3 0.35942773255605687 1107 4 0.2796502165231516 1107 5 0.19693261134433016 1107 6 0.20794654721883887 1107 7 0.198110178879098 1107 8 0.2606066943512033 1107 9 0.24409264415724152 1107 10-11 0.251723215518183 1107 12-13 0.35011925733365246 1107 14-15 0.4037980705577269 1107 16-17 0.5325218324437344 1107 18-19 0.38107988041799246 1107 20-21 0.45224676337826963 1107 22-23 0.3382607507422506 1107 24-25 0.5317484427750898 1107 26-27 0.42368205526839375 1107 28-29 0.5522131167491153 1107 30-31 0.5890476245053051 1107 32-33 0.4414794802071711 1107 34-35 0.7186372786393775 1107 36-37 0.612077642913178 1107 38-39 0.5219660761076526 1107 40-41 0.7526351452429552 1107 42-43 0.7340081777118215 1107 44-45 0.9336659572641182 1107 46-47 0.75448137196733 1107 48-49 1.0579504328499567 1107 50-51 0.7290906985408654 1107 52-53 0.9534364431992657 1107 54-55 0.9203430141137581 1107 56-57 0.913109288746238 1107 58-59 0.856883428588084 1107 60-61 0.9050673127250057 1107 62-63 0.891071676390748 1107 64-65 0.9741355685039288 1107 66-67 1.0404999516706255 1107 68-69 0.6724180344925585 1107 70-71 0.527625369636123 1107 72-73 0.8477389545421339 1107 74-75 0.7320067255073432 1107 76-77 0.46464710228358896 1107 78-79 0.831314928392537 1107 80-81 0.8167541161968508 1107 82-83 1.0575667430322504 1107 84-85 0.7331736937143027 1107 86-87 1.1989404826228949 1107 88-89 1.2293194535452123 1107 90-91 0.9328003854350335 1107 92-93 1.0837365006916322 1107 94-95 1.3767152703184848 1107 96-97 1.1545853194942843 1107 98-99 1.1552220621601563 1107 100-101 1.0393153387837089 1108 1 0.0720600003712093 1108 2 0.12295803123466698 1108 3 0.10139765330094619 1108 4 0.15983001569813382 1108 5 -0.026950405434163827 1108 6 0.10207598885008196 1108 7 0.09484792351148741 1108 8 0.10104827567697328 1108 9 0.2589109470848783 1108 10-11 0.07821496092211788 1108 12-13 0.059794935498239 1108 14-15 0.03622584308398302 1108 16-17 0.07025149060165603 1108 18-19 0.1868102536247136 1108 20-21 0.07780554035076648 1108 22-23 0.01000343842031981 1108 24-25 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-1.696048736949404 2316 82-83 -1.8522580319301127 2316 84-85 -1.455842907816919 2316 86-87 -1.6587765999496398 2316 88-89 -2.148716085317158 2316 90-91 -1.8267683858541162 2316 92-93 -2.0328182477894323 2316 94-95 -2.050324350187438 2316 96-97 -1.9328396679591364 2316 98-99 -2.479314675301268 2316 100-101 -2.545363748999659 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 30.0 4 41.0 5 103.0 6 224.0 7 578.0 8 1249.0 9 2139.0 10 3450.0 11 5419.0 12 7492.0 13 10478.0 14 13584.0 15 17432.0 16 21869.0 17 26394.0 18 24362.0 19 20326.0 20 20040.0 21 21355.0 22 23164.0 23 25544.0 24 29396.0 25 33623.0 26 39808.0 27 47152.0 28 54232.0 29 63289.0 30 73308.0 31 84178.0 32 97255.0 33 112266.0 34 128398.0 35 140772.0 36 138131.0 37 94474.0 38 22263.0 39 376.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.68677659460807 22.910146372734072 16.24851312368674 20.154563908971117 2 18.590399602327636 24.0564005381111 36.2315558432698 21.121644016291462 3 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20.601486624827526 22-23 22.265442370122646 26.60736992145953 30.377113198766114 20.750074509651714 24-25 22.103450915739828 26.841868191149004 30.35506171696544 20.69961917614572 26-27 22.027737590670245 26.932254091399667 30.21217487166139 20.827833446268702 28-29 21.684813455661846 27.03157035658102 30.40866060816253 20.8749555795946 30-31 21.916175639048056 26.77150943100617 30.370574520117533 20.941740409828245 32-33 21.84426401708592 27.00611096591148 30.200570147920843 20.94905486908176 34-35 22.183196386834336 26.874771221722295 30.090948712361076 20.851083679082294 36-37 22.324167841729693 26.660114800096853 30.05690153684001 20.95881582133345 38-39 22.065356889009806 26.91798197571978 30.083448115740552 20.933213019529866 40-41 22.240627435787783 26.881020145756473 29.90222487459866 20.976127543857086 42-43 22.176430959166726 27.05480281270875 29.63552860778893 21.133237620335592 44-45 22.386472765962143 27.116674779294392 29.27464562297325 21.22220683177021 46-47 22.514912448119777 27.465788251782513 28.76742278143507 21.251876518662637 48-49 22.665579450184183 27.67028854559277 28.38486039178962 21.279271612433437 50-51 22.482366882783925 27.870224245393736 28.194364524105243 21.453044347717096 52-53 22.815746416534772 27.948304808635566 27.89147499363513 21.34447378119453 54-55 22.64641453698125 27.759724768159476 27.98896594354927 21.60489475131 56-57 22.611586986432094 28.072897230870904 27.833400750322607 21.4821150323744 58-59 22.84567925192753 27.837220671907865 27.855166890935056 21.46193318522955 60-61 22.6652271479369 27.799387967152196 28.248584754157786 21.286800130753118 62-63 22.480056231386172 27.73472829330337 28.38606094012383 21.399154535186632 64-65 22.47566490872182 27.830862703332475 28.181027370457056 21.51244501748865 66-67 22.452440790003113 28.097766908774197 27.88173596724676 21.568056333975928 68-69 22.43327594586944 28.002221909667263 27.86381545331052 21.700686691152775 70-71 22.520745026321187 27.957964437911624 27.777184316674642 21.744106219092547 72-73 22.642518539988586 27.90720943572988 27.68864996978695 21.761622054494588 74-75 22.525306081898876 28.238122690565206 27.406200938141957 21.83037028939397 76-77 22.623305260369264 28.144463957362138 27.516525447265984 21.715705335002614 78-79 22.625687582075436 28.06883796848873 27.426050209514912 21.879424239920922 80-81 22.44855156736385 28.270626197769076 27.45046372491139 21.830358509955683 82-83 22.529345052957297 28.254251983784336 27.439124482133032 21.77727848112534 84-85 22.494389008117643 28.238057747548627 27.27691611411349 21.990637130220232 86-87 22.638549973988663 28.050189592546054 27.356340338649428 21.954920094815858 88-89 22.56876924846377 28.065964923775283 27.17711275090881 22.188153076852135 90-91 22.50350560719111 28.200713726668752 27.243357150334823 22.052423515805323 92-93 22.562523233902912 28.101200827803485 27.1654709663452 22.1708049719484 94-95 22.464673785320873 27.98903715857735 27.075170310739654 22.47111874536212 96-97 22.529076236837223 28.046288393397933 26.982192307130802 22.44244306263404 98-99 22.379267799820894 28.130544779294866 26.780088912310813 22.710098508573434 100-101 22.445042345239756 28.297388069267864 26.617608099292678 22.6399614861997 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 559.0 1 400.5 2 271.5 3 320.5 4 321.5 5 351.5 6 554.0 7 1036.0 8 1666.5 9 2014.0 10 1909.0 11 1705.0 12 1725.5 13 1910.5 14 2143.5 15 2368.0 16 2572.5 17 2810.0 18 3033.5 19 3248.5 20 3573.0 21 4055.0 22 4625.0 23 5395.5 24 6546.5 25 7996.5 26 9806.0 27 11877.5 28 13785.5 29 15754.5 30 18312.0 31 21200.5 32 23831.5 33 26471.0 34 29517.0 35 31909.5 36 34584.0 37 37495.5 38 39535.0 39 40756.5 40 41843.0 41 44009.0 42 46206.5 43 47638.5 44 48885.0 45 50247.5 46 50811.5 47 51766.5 48 54196.5 49 54168.0 50 51291.0 51 48751.0 52 46537.5 53 44729.5 54 42936.5 55 39881.0 56 36303.5 57 32748.0 58 29147.0 59 25741.0 60 22338.0 61 18496.0 62 14582.5 63 11403.0 64 8729.0 65 6531.0 66 4873.0 67 3672.0 68 2604.0 69 1713.5 70 1139.0 71 770.5 72 577.5 73 408.5 74 283.0 75 214.0 76 146.0 77 87.0 78 45.0 79 30.5 80 19.0 81 8.0 82 8.0 83 5.0 84 3.0 85 2.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01766132696386122 2 0.0019228057581623104 3 2.848601123203423E-4 4 0.0 5 0.002350095926642824 6 0.0 7 1.4243005616017116E-4 8 0.0 9 7.121502808008558E-5 10-11 0.0 12-13 0.0049138369375259046 14-15 0.002741778581083294 16-17 0.0 18-19 2.1364508424025672E-4 20-21 7.477577948408985E-4 22-23 0.0012462629914014975 24-25 3.9168265444047064E-4 26-27 1.7803757020021393E-4 28-29 7.121502808008558E-5 30-31 0.0 32-33 7.121502808008558E-5 34-35 0.0 36-37 0.0012818705054415402 38-39 0.006551782583367872 40-41 4.628976825205562E-4 42-43 0.0 44-45 6.053277386807273E-4 46-47 1.4243005616017116E-4 48-49 3.560751404004279E-5 50-51 0.0015667306177618826 52-53 3.9168265444047064E-4 54-55 0.0 56-57 1.4243005616017116E-4 58-59 1.7803757020021393E-4 60-61 0.0017803757020021392 62-63 0.0 64-65 1.0682254212012836E-4 66-67 7.121502808008558E-5 68-69 3.560751404004279E-5 70-71 4.2729016848051343E-4 72-73 2.4925259828029953E-4 74-75 0.00281299360916338 76-77 0.0 78-79 1.4243005616017116E-4 80-81 0.0017803757020021392 82-83 3.560751404004279E-5 84-85 0.0024569184687629523 86-87 1.7803757020021393E-4 88-89 0.001958413272202353 90-91 0.001353085533521626 92-93 0.0 94-95 0.0 96-97 1.0682254212012836E-4 98-99 3.9168265444047064E-4 100-101 0.0018515907300822249 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1404198.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.877896818176836 #Duplication Level Percentage of deduplicated Percentage of total 1 80.74880873340861 50.77315263729963 2 11.488769551939763 14.447793329093606 3 3.4420822004673717 6.492926683220116 4 1.4557058192182517 3.661268811936993 5 0.7667436924728971 2.4105615390649366 6 0.47099438199910304 1.776908169196834 7 0.2929937818691339 1.2895982949314369 8 0.20539173545774583 1.0331680279534732 9 0.1717794836503429 0.9721019383601361 >10 0.8729106091712848 10.002431656559803 >50 0.055996994828674485 2.4159918685502544 >100 0.026573849176539117 2.956192977596483 >500 5.678028819545665E-4 0.2203542933238477 >1k 4.542423055636532E-4 0.5887301193956982 >5k 2.271211527818266E-4 0.9588196535167295 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7233 0.5150982981032589 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6195 0.44117709895613016 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4093 0.2914831099317903 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1580 0.1125197443665352 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1521 0.10831805770981015 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.121502808008557E-5 2 0.0 0.0 0.0 0.0 1.4243005616017114E-4 3 0.0 0.0 0.0 0.0 1.4243005616017114E-4 4 0.0 0.0 0.0 0.0 1.4243005616017114E-4 5 0.0 0.0 0.0 0.0 1.4243005616017114E-4 6 0.0 0.0 0.0 0.0 1.4243005616017114E-4 7 0.0 0.0 0.0 0.0 1.4243005616017114E-4 8 0.0 0.0 0.0 0.0 1.4243005616017114E-4 9 0.0 0.0 0.0 0.0 1.4243005616017114E-4 10-11 0.0 0.0 0.0 3.5607514040042784E-5 1.4243005616017114E-4 12-13 0.0 0.0 0.0 2.1364508424025672E-4 1.4243005616017114E-4 14-15 0.0 3.5607514040042784E-5 0.0 2.1364508424025672E-4 1.7803757020021393E-4 16-17 0.0 7.121502808008557E-5 0.0 2.492525982802995E-4 2.1364508424025672E-4 18-19 0.0 7.121502808008557E-5 0.0 3.2046762636038506E-4 2.1364508424025672E-4 20-21 0.0 7.121502808008557E-5 0.0 4.628976825205562E-4 2.1364508424025672E-4 22-23 0.0 7.121502808008557E-5 0.0 8.545803369610268E-4 2.1364508424025672E-4 24-25 0.0 7.121502808008557E-5 0.0 0.001495515589681797 2.1364508424025672E-4 26-27 0.0 7.121502808008557E-5 0.0 0.001958413272202353 2.1364508424025672E-4 28-29 0.0 7.121502808008557E-5 0.0 0.003489536375924193 2.1364508424025672E-4 30-31 0.0 7.121502808008557E-5 0.0 0.0056972022464068465 2.1364508424025672E-4 32-33 0.0 7.121502808008557E-5 0.0 0.010860291782213049 2.1364508424025672E-4 34-35 0.0 7.121502808008557E-5 0.0 0.016165811374179426 2.1364508424025672E-4 36-37 0.0 7.121502808008557E-5 0.0 0.024747222257829736 2.1364508424025672E-4 38-39 3.5607514040042784E-5 7.121502808008557E-5 0.0 0.03849172267728625 2.1364508424025672E-4 40-41 7.121502808008557E-5 7.121502808008557E-5 0.0 0.056722769865788156 2.1364508424025672E-4 42-43 7.121502808008557E-5 7.121502808008557E-5 0.0 0.07417045174540912 2.1364508424025672E-4 44-45 7.121502808008557E-5 7.121502808008557E-5 0.0 0.09471598734651382 2.1364508424025672E-4 46-47 7.121502808008557E-5 7.121502808008557E-5 0.0 0.1252316268788305 2.1364508424025672E-4 48-49 7.121502808008557E-5 7.121502808008557E-5 0.0 0.15350399302662443 2.1364508424025672E-4 50-51 7.121502808008557E-5 7.121502808008557E-5 0.0 0.18302262216581994 2.1364508424025672E-4 52-53 7.121502808008557E-5 7.121502808008557E-5 0.0 0.2102267628924126 2.492525982802995E-4 54-55 7.121502808008557E-5 7.121502808008557E-5 0.0 0.2415969827616903 2.8486011232034227E-4 56-57 7.121502808008557E-5 7.121502808008557E-5 0.0 0.29069974462290926 2.8486011232034227E-4 58-59 7.121502808008557E-5 7.121502808008557E-5 0.0 0.3373811955294054 2.8486011232034227E-4 60-61 7.121502808008557E-5 7.121502808008557E-5 0.0 0.38046628751785716 3.2046762636038506E-4 62-63 7.121502808008557E-5 7.121502808008557E-5 0.0 0.42372941707650913 3.5607514040042785E-4 64-65 7.121502808008557E-5 7.121502808008557E-5 0.0 0.4723336737411675 3.5607514040042785E-4 66-67 7.121502808008557E-5 7.121502808008557E-5 0.0 0.5302670990843172 4.272901684805134E-4 68-69 7.121502808008557E-5 7.121502808008557E-5 0.0 0.5968531503391972 5.341127106006417E-4 70-71 7.121502808008557E-5 7.121502808008557E-5 0.0 0.6738722032078097 5.697202246406845E-4 72-73 7.121502808008557E-5 7.121502808008557E-5 0.0 0.7553778028454676 7.121502808008557E-4 74-75 7.121502808008557E-5 7.121502808008557E-5 0.0 0.8481353769197791 7.833653088809413E-4 76-77 7.121502808008557E-5 7.121502808008557E-5 0.0 0.9535692259923458 7.833653088809413E-4 78-79 7.121502808008557E-5 7.121502808008557E-5 0.0 1.0698277593330854 7.833653088809413E-4 80-81 7.121502808008557E-5 7.121502808008557E-5 0.0 1.195878359034837 7.833653088809413E-4 82-83 7.121502808008557E-5 7.121502808008557E-5 0.0 1.3214660610540678 7.833653088809413E-4 84-85 7.121502808008557E-5 7.121502808008557E-5 0.0 1.4582701299959124 7.833653088809413E-4 86-87 7.121502808008557E-5 7.121502808008557E-5 0.0 1.5955014891062371 7.833653088809413E-4 88-89 7.121502808008557E-5 7.121502808008557E-5 0.0 1.744376505307656 7.833653088809413E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5355 0.0 38.85476 1 GTATCAA 8260 0.0 33.75885 1 TATCAAC 10170 0.0 27.134567 2 ATCAACG 10480 0.0 26.24128 3 TCAACGC 10480 0.0 26.24128 4 CAACGCA 10665 0.0 25.786085 5 AACGCAG 11010 0.0 24.981634 6 ACGGTAT 120 1.7092926E-4 23.75366 8 GAGTACT 7175 0.0 21.848888 12-13 ACGCAGA 12540 0.0 21.744225 7 CGCAGAG 12610 0.0 21.587387 8 GTACTTT 7515 0.0 20.574091 14-15 AGTACTT 7450 0.0 20.277208 12-13 GCAGAGT 13045 0.0 20.212006 9 AGAGTAC 10595 0.0 20.043201 10-11 TACTTTT 8340 0.0 17.855423 14-15 ACTTTTT 8795 0.0 17.79583 16-17 CAGAGTA 12720 0.0 17.684525 10-11 GAGTACA 5530 0.0 17.438154 1 AGTACAT 5000 0.0 17.383965 2 >>END_MODULE