FastQCFastQC Report
Thu 26 May 2016
SRR937059_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937059_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2426084
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87010.3586438062325954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57120.2354411471325807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39890.1644213473235057No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31830.13119908461537194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA116150.030.8148541
GGTATCA78650.028.525131
GTACATG101050.026.2001191
TACATGG99700.025.7931522
ATCAACG140450.025.3121093
TCAACGC140700.025.2006174
CAACGCA141400.025.1410125
TATCAAC141900.025.0900612
AACGCAG146150.024.3239086
ACATGGG103500.023.6032223
ACGCAGA162800.021.6026927
CGCAGAG162600.021.6004818
CATGGGG53750.021.2226314
GAGTACT77450.020.85665312-13
GTGTAGC14350.020.5363921
AGAGTAC127800.020.39536710-11
AGTACTT81600.019.91237412-13
GCAGAGT173100.019.74119
GTACTTT82600.019.32599414-15
TAGCCCT15350.018.2688034
Produced by FastQC (version 0.11.5)